Characterization of cells of the myeloid-monocytic lineage (ML-1, HL-60, THP-1, U-937) chronically infected with the human immunodeficiency virus-1. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Characterization of cells of the myeloid-monocytic lineage (ML-1, HL-60, THP-1, U-937) chronically infected with the human immunodeficiency virus-1.

Pathobiology. 1993;61(3-4):145-53. Unique Identifier : AIDSLINE MED/94030597
Ushijima H; Kunisada T; Ami Y; Tsuchie H; Takahashi I; Schacke H; Muller WE; Division of AIDS Virus, National Institute of Health, Tokyo,; Japan.

Abstract: The myeloid-monocytic cells ML-1, HL-60, THP-1 and U-937 were chronically infected (for more than 2 years) with the lymphotropic HIV-1 strain HTLV-IIIB. Reinfection experiments revealed that viruses obtained from chronically infected ML-1/HIV-1 and HL-60/HIV-1 cells show a low infectivity if tested with uninfected ML-1 and HL-60 cells in contrast to virus preparations from chronically infected THP-1/HIV-1 and U-937/HIV-1 with their corresponding uninfected cell lines. Analyses of selected cell surface markers showed a differential expression of CD4, CD8, CD11c, CD14, CD15, CD20, HLA-DR and HLA-DQ in non- or chronically infected cells. During chronical infection, the myeloid-monocytic cells lost their reactivity with peroxidase and esterase. In chronically infected cells, the steady-state levels for TNF-alpha mRNA remained unchanged while those for IL-6 decreased. The half-lives of transcripts of both TNF-alpha (t1/2: 70 min) and IL-6 (t1/2: 100 min) were nearly the same in uninfected and chronically infected HL-60 cells.
Keywords: Bone Marrow/*MICROBIOLOGY Cell Line Flow Cytometry Fluorescent Antibody Technique Gene Expression Human HIV Infections/IMMUNOLOGY/*PATHOLOGY HIV-1/*GROWTH & DEVELOPMENT Immunophenotyping Interleukin-6/GENETICS Monocytes/*MICROBIOLOGY RNA, Messenger/METABOLISM Support, Non-U.S. Gov't Time Factors Tumor Necrosis Factor/GENETICS JOURNAL ARTICLEKWDbonemarrow/KWDmicrobiologycelllineflowcytometryfluorescentantibodytechniquegeneexpressionhumanhivinfections/immunology/KWDpathologyhiv-1/KWDgrowth&developmentimmunophenotypinginterleukin-6/geneticsmonocytes/KWDmicrobiologyrna,messenger/metabolismsupport,non-uKWDsKWDgov'ttimefactorstumornecrosisfactor/geneticsjournalarticle
940228
M9420784

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