Characterization of dual HIV-1 and HIV-2 serological profiles by polymerase chain reaction. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Characterization of dual HIV-1 and HIV-2 serological profiles by polymerase chain reaction.

AIDS. 1993 Sep;7(9):1185-9. Unique Identifier : AIDSLINE GENBANK/M15390
Leonard G; Chaput A; Courgnaud V; Sangare A; Denis F; Brechot C; Laboratoire de Virologie, CHU Dupuytren, Limoges, Paris, France.

Abstract: OBJECTIVE: To evaluate the frequency of dual HIV-1 and HIV-2 DNA sequences in patients with dual serological profiles. DESIGN: We tested 40 samples from AIDS patients living in Abidjan, Cote d'Ivoire. METHODS: Dual serological reactivity was determined by double Western blot and two enzyme-linked immunosorbent assays with recombinant proteins and synthetic peptides as antigens. The Western blot was considered to show dual reactivity when sera reacted with at least two glycoproteins and one core protein of each virus. HIV DNA sequences were detected by hybridization to radiolabelled probes of polymerase chain reaction (PCR) products amplified using specific primers. RESULTS: Both HIV-1 and HIV-2 DNA sequences were detected in four out of 11 samples with a dual serological profile and in four out of 24 samples with anti-HIV-1 antibodies only. CONCLUSION: These results show that dual HIV-1 and HIV-2 serological profiles are not always due to infection by both viruses, and emphasize the need for a combination of serological and PCR assays for the appraisal of these viral infections.
Keywords: AIDS Serodiagnosis Base Sequence Blotting, Western Cote d'Ivoire/EPIDEMIOLOGY DNA, Viral Enzyme-Linked Immunosorbent Assay Human HIV Infections/EPIDEMIOLOGY/*MICROBIOLOGY HIV-1/*GENETICS/IMMUNOLOGY HIV-2/*GENETICS/IMMUNOLOGY Molecular Sequence Data Polymerase Chain Reaction Support, Non-U.S. Gov't JOURNAL ARTICLEKWDaidsserodiagnosisbasesequenceblotting,westerncoted'ivoire/epidemiologydna,viralenzyme-linkedimmunosorbentassayhumanhivinfections/epidemiology/KWDmicrobiologyhiv-1/KWDgenetics/immunologyhiv-2/KWDgenetics/immunologymolecularsequencedatapolymerasechainreactionsupport,non-uKWDsKWDgov'tjournalarticle
940228
M9420773

Copyright © 1994 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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