Genetic heterogeneity of the V3 region of the HIV-1 envelope glycoprotein in Brazil. Brazilian Collaborative AIDS Research Group.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Genetic heterogeneity of the V3 region of the HIV-1 envelope glycoprotein in Brazil. Brazilian Collaborative AIDS Research Group.

AIDS. 1993 Sep;7(9):1191-7. Unique Identifier : AIDSLINE MED/94030749
Potts KE; Kalish ML; Lott T; Orloff G; Luo CC; Bernard MA; Alves CB; Badaro R; Suleiman J; Ferreira O; et al; Division of HIV/AIDS, Centers for Disease Control and Prevention,; Atlanta, Georgia.

Abstract: OBJECTIVE: To examine the genetic heterogeneity of the V3 region of HIV-1 gp120 from 22 Brazilian HIV-1 specimens. DESIGN: Genetic heterogeneity was examined by DNA sequencing of the C2 V3 region of the HIV-1 envelope (env) gene from polymerase chain reaction (PCR)-amplified HIV-1 DNA. Deduced amino-acid sequences were compared to determine the extent of amino-acid conservation among the Brazilian specimens. Genetic similarity among and between the Brazilian specimens and other previously published HIV-1 isolates was analyzed by principal co-ordinate and DNA parsimony methods. METHODS: A 282 base pair (bp) region of a 1.5 kilo (k) bp PCR-amplified HIV-1 env fragment was sequenced by a Taq dye-labeled primer cycle sequencing reaction. Nucleotide sequences were used to analyze inter-specimen relationships based on overall nucleotide sequence similarity and DNA parsimony principles. RESULTS: Amino-acid comparison showed that 15 of the 35 (43%) residues of the V3 loop were conserved among the Brazilian specimens. Nine of the 22 (40%) Brazilian specimens contained the North American-European GPGR tetrapeptide motif, while eight (36%) contained the GWGR motif, previously reported in Japanese isolates. Principal co-ordinate analysis demonstrated that 19 of the 20 examined Brazilian HIV-1 specimens were more similar to North American and Haitian isolates than to African isolates. Similar results were also obtained by DNA parsimony analysis. CONCLUSION: The majority of the Brazilian specimens examined are more genetically related to North American and Haitian HIV-1 isolates than to African isolates. This finding and the presence of a GWGR V3 loop motif in some Brazilian isolates may be important for vaccine development.

Keywords: Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Amino Acid Sequence Brazil DNA, Viral Human HIV Envelope Protein gp120/*GENETICS HIV-1/*GENETICS Molecular Sequence Data Peptide Fragments/*GENETICS Polymerase Chain Reaction Sequence Homology, Amino Acid Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
940228
M9420772

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