The endothelium in HIV-associated Kaposi's sarcoma.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

The endothelium in HIV-associated Kaposi's sarcoma.

Diss Abstr Int [B]; 54(6):3004 1993. Unique Identifier : AIDSLINE ICDB/94605793
Corbeil J; Univ. of New South Wales, Australia

Abstract: The aim of this project was to culture Kaposi's sarcoma-derived (KS) cells in order to investigate its etiology and attempt to elucidate the role of the vascular endothelial cell (EC) in this pathological process. The establishment of five cell cultures derived from pleural or peritoneal fluid of HIV-infected individuals with KS, and one without, are described. These cells originate from vascular endothelium since specific staining for EC markers and the secretion of endothelin, a vascular EC product were demonstrated. The KS-derived cells secreted large amounts of cytokines (GM-CSF, TNF-alpha, IL-1 beta and especially IL-6). Conditioned media from the KS-derived cells prompted normal capillary EC to proliferate. The KS-derived cells synthesized fibroblast growth factor (FGF)-like molecules in amounts sufficient to induce the proliferation of normal EC and fibroblasts. These data support the existence of a paracrine pathway of EC proliferation in KS and suggest that KS-derived cells could sustain their own growth via an autocrine mechanism. Furthermore, we studied the effect of in vitro infection of vascular EC with HIV-1. Low levels of viral antigen ranging from 30 to 225 pg/mL were demonstrated and the expression was transient. Immunogold electron microscopy using monoclonal antibodies specific for p18, p24 and gp41 showed the presence of HIV. Levels of IL-6 were increased at day 3 of the infection compared to EC inoculated with Hut78 supernatant or DEAE-dextran treated EC. These results are the first description that EC can be productively infected in vitro and that viral infection of EC induces the production of IL-6. The relationship between the HIV-infection of EC and KS cells secretory capability could possibly lead us to a clearer picture of the neopathogenesis of KS. (Full text NOT AVAILABLE FROM UNIVERSITY MICROFILMS INT'L.)

Keywords: Acquired Immunodeficiency Syndrome/*COMPLICATIONS Cell Division Culture Media, Conditioned Cytokines/SECRETION Endothelium, Vascular/MICROBIOLOGY/*PATHOLOGY/SECRETION HIV-1/ISOLATION & PURIF Microscopy, Electron Sarcoma, Kaposi's/*ETIOLOGY/PATHOLOGY Tumor Cells, Cultured THESIS
941230
M94C4341

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