Growth suppression and toxicity induced by caffeic acid phenethyl ester (CAPE) in type 5 adenovirus transformed rat embryo cells correlates directly with transformation progression (Meeting abstract).

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Growth suppression and toxicity induced by caffeic acid phenethyl ester (CAPE) in type 5 adenovirus transformed rat embryo cells correlates directly with transformation progression (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 35:A979 1994. Unique Identifier : AIDSLINE ICDB/94602665
Su ZZ; Lin J; Grunberger D; Fisher PB; Columbia University, College of Physicians and Surgeons, New; York, NY 10032

Abstract: The active component of the honeybee hive product propolis, CAPE, induces a selective growth suppressive and toxic effect toward cloned rat embryo fibroblast (CREF) cells transformed by adenovirus type 5 (Ad5) or the Ad5 E1A transforming gene versus untransformed CREF cells. The present study was conducted to determine if CAPE-induced growth suppression/toxicity was a direct result of expression of the Ad5 E1A and E1B transforming genes or a consequence of the action of these genes resulting in the transformed state. For this investigation we used somatic cell hybrids and 5-azacytidine treated Ad5-transformed rat embryo cells that display different stages of expression of the transformed phenotype. This series of cell lines has permitted us to determine if expression of the transformed state and the stage of transformation progression regulates CAPE sensitivity. Evidence is presented indicating that sensitivity to CAPE is directly determined by the state of expression of the transformed progression phenotype, as opposed to simply the expression of the Ad5 E1A and E1B transforming genes. These results provide further evidence that CAPE may represent a unique compound that can specifically target progressed transformed cells for growth suppression and toxicity. An understanding of the mechanism underlying this selective effect of CAPE could result in the identification of important biochemical pathways mediating cellular transformation and progression of the transformed state.

Keywords: Adenoviridae/GENETICS Adenovirus E1A Proteins/GENETICS Adenovirus E1B Proteins/GENETICS Animal Azacitidine/PHARMACOLOGY Caffeic Acids/*TOXICITY Cell Line, Transformed Cell Transformation, Viral Cytotoxins/*TOXICITY Hybrid Cells Phenotype Phenylethyl Alcohol/*ANALOGS & DERIVATIVES/TOXICITY Rats ABSTRACT
941230
M94C4320

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