Lymphocyte subset analysis by Boolean algebra: a phenotypic approach using a cocktail of 5 antibodies and 3 colour immunofluorescence. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Lymphocyte subset analysis by Boolean algebra: a phenotypic approach using a cocktail of 5 antibodies and 3 colour immunofluorescence.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 52). Unique Identifier : AIDSLINE ASHM5/94348912
Hunter S; Peters L; Wotherspoon J; Crowe S; Flow Cytometry Unit, Macfarlane Burnet Centre for Medical; Research, Fairfield Hospital, Victoria, Australia.

Abstract: A rapid method has been developed whereby total CD3+ T-cells, CD4+ T-cells (CD3+CD4+), CD8+ T-cells (CD3+CD8+), putative gamma delta-receptor-T-cells (CD3+CD4-CD8-) and T-cells that are CD3+CD4+CD8+ as well as B-lymphocytes and NK-cells can be enumerated after staining in a single tube. Whole blood specimens are labelled with a mixture of antibodies: FITC-CD4 and CD19, PE-CD8 and CD16, and either peridinin chlorophyll protein (PerCP) or allophycocyanin (APC) labelled CD3 for use on a Becton Dickinson FACScan or FACStar Plus flow cytometer respectively. Data were analysed with LYSYS-II software package and all of the lymphocyte subset values were determined by Boolean algebra. Our study has shown that this new procedure is statistically equivalent to a standard analysis procedure (SimulSET lymphocyte subset analysis), is less time consuming and more cost effective.
Keywords: B-Lymphocyte Subsets/*IMMUNOLOGY Flow Cytometry *Fluorescent Antibody Technique Human Immunophenotyping/*METHODS Leukocyte Count/*METHODS Software T-Lymphocyte Subsets/*IMMUNOLOGY ABSTRACTKWDb-lymphocytesubsets/KWDimmunologyflowcytometryKWDfluorescentantibodytechniquehumanimmunophenotyping/KWDmethodsleukocytecount/KWDmethodssoftwaret-lymphocytesubsets/KWDimmunologyabstract
941230
M94C4315

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