Mechanisms of AZT resistance in HIV isolates.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Mechanisms of AZT resistance in HIV isolates.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 53). Unique Identifier : AIDSLINE ASHM5/94348913
Buchanan L; Tachedjian G; Birch C; Virology Department, Fairfield Hospital, Victoria.

Abstract: AIM: To investigate the susceptibility of the reverse transcriptase (RT) and ribonuclease H (RNase H) from AZT-sensitive (AZT-S) and AZT-resistant (AZT-R) HIV isolates to AZT-triphosphate (AZTTP) and AZT-monophosphate (AZTMP). METHODS: Isolates obtained from a patient before and after long-term AZT therapy were examined for susceptibility to AZT. RT and RNase H from these isolates were analysed in in vitro assays to determine their susceptibilities to AZTTP and AZTMP respectively. RESULTS AND CONCLUSIONS: Amino acid changes occurring in the HIV RT after long-term AZT therapy result in phenotypic AZT resistance. However, altered susceptibility cannot be definitely associated with the effects of AZTTP on RT or AZTMP on RNase H using enzyme assays, suggesting that the assay procedures used are not optimal or that alternative mechanisms of action are operating for AZT.

Keywords: Drug Resistance, Microbial/GENETICS Human HIV/*DRUG EFFECTS/GENETICS Mutation/GENETICS Phenotype Ribonuclease H, Calf Thymus/GENETICS RNA-Directed DNA Polymerase/GENETICS Zidovudine/*PHARMACOLOGY ABSTRACT
941230
M94C4314

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