Characterisation of HIV-1 LTR DNA-binding proteins. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Characterisation of HIV-1 LTR DNA-binding proteins.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 57). Unique Identifier : AIDSLINE ASHM5/94348916
Peters A; Deacon NJ; AIDS Molecular Biology Laboratory, Macfarlane Burnet Centre for; Medical Research, Fairfield, Vic., Australia.

Abstract: An investigation into the characterisation of mammalian nuclear proteins which bind to the HIV-1 LTR U3 region has been carried out using a series of overlapping 40-mer oligonucleotides. These oligonucleotides were synthesised to correspond to the HXB2 sequence from n.t. -210 to n.t. -454. Two oligonucleotides have shown a number of specific binding interactions in mobility shift assays, U.V. cross-linked mobility shift assays and south-western analysis. Oligonucleotide 7 (n.t. -210 to n.t. -250) has at least 4 specific interactions and oligonucleotide 13(n.t. -410 to n.t. 454) has at least 2 specific interactions. The region of oligonucleotide 7 is thought to bind ILF (Li et al, 1991) and possibly members of the ets family. No protein binding has been reported in the region of oligonucleotide 13. Reference: Li P, et al. 1991 P.N.A.S. USA 88; 7739-7743.
Keywords: DNA-Binding Proteins/*GENETICS Human HIV-1/*GENETICS Oligonucleotides/GENETICS Repetitive Sequences, Nucleic Acid/*GENETICS ABSTRACTKWDdna-bindingproteins/KWDgeneticshumanhiv-1/KWDgeneticsoligonucleotides/geneticsrepetitivesequences,nucleicacid/KWDgeneticsabstract
941230
M94C4311

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