Role the immunodeficiency virus nef gene and the production in CNS tropism. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

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Role the immunodeficiency virus nef gene and the production in CNS tropism.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 56). Unique Identifier : AIDSLINE ASHM5/94348917
Smith K; Deacon NJ; McPhee DA; Brew B; AIDS Molecular Biology Unit, Macfarlane Burnet Centre, Vic.,; Australia.

Abstract: Particular strains of HIV may dominate the CNS of infected individuals and it is possible that the HIV Nef protein plays a role in differentiating CNS and peripheral blood isolates. To address this, 6 pairs of coincident isolates from the CSF and peripheral blood of patients with AIDS or ARC were analysed for their ability to replicate in primary blood mononuclear cells and primary macrophages. Peripheral blood isolates appeared to have enhanced replication in all the cell types examined but the replicative abilities of paired isolates were very similar. CSF isolates were less likely to induce CPE and syncytia in PBMC and macrophage cultures. Isolates were examined for their ability to express the Nef protein by flow cytometry and immunoblotting and the nef gene of isolates was PCR amplified and sequenced. Most isolates appeared to express Nef and sequence analysis revealed little difference in nef between CSF and blood isolates. No differences between Nef of CSF and peripheral blood isolates were apparent by parameters used in this study.
Keywords: Acquired Immunodeficiency Syndrome/MICROBIOLOGY AIDS Dementia Complex/*MICROBIOLOGY AIDS-Related Complex/MICROBIOLOGY Brain/MICROBIOLOGY Cerebrospinal Fluid/MICROBIOLOGY Cytopathogenic Effect, Viral/GENETICS Flow Cytometry Genes, nef/*GENETICS Human HIV/*GENETICS Immunoblotting Polymerase Chain Reaction Sequence Analysis Virus Replication/GENETICS ABSTRACTKWDacquiredimmunodeficiencysyndrome/microbiologyaidsdementiacomplex/KWDmicrobiologyaids-relatedcomplex/microbiologybrain/microbiologycerebrospinalfluid/microbiologycytopathogeniceffect,viral/geneticsflowcytometrygenes,nef/KWDgeneticshumanhiv/KWDgeneticsimmunoblottingpolymerasechainreactionsequenceanalysisvirusreplication/geneticsabstract
941230
M94C4310

Copyright © 1994 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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