Primary T-cell response to HIV-1 in vitro.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Primary T-cell response to HIV-1 in vitro.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:105 (poster no. 60). Unique Identifier : AIDSLINE ASHM5/94348920
Todd B; Pope J; NCHVR, Royal Children's Hospital, Brisbane, Qld.

Abstract: HIV-I-specific, MHC-restricted cytotoxic T cells may be detected directly in peripheral blood (PB) of HIV-1-infected patients, and cytotoxic T-cell lines can be established in vitro. CD8+ T cells predominate, and epitopes exist in most viral antigens. Generation of cytotoxic T cells from the PB mononuclear cells from uninfected donors has received less attention. Our aim is to characterise the primary cellular immune response to HIV-1 infection in vitro. The approach is to generate T-cell colonies and amplify T-cell lines and clones. These T-cells are phenotyped (cell markers) and their HIV-1 specificity determined by use of autologous targets infected with HIV-1 or with vaccinia constructs expressing HIV-1 antigens. So far, 92 T-cell lines and 20 clones have been established in response to stimulation with live or inactivated HIV-1. Both CD4+ and CD8+ T cells have been generated. Cytotoxicity tests have so far yielded 6 T-cell lines with putative HIV-1 specificity for detailed study.

Keywords: Cell Line Cytotoxicity Tests, Immunologic Human HIV-1/*IMMUNOLOGY Immunophenotyping In Vitro T-Lymphocytes/*IMMUNOLOGY T-Lymphocytes, Cytotoxic/*IMMUNOLOGY ABSTRACT
941230
M94C4307

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1994. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1994. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .