HIV regulatory and accessory gene mRNAs.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

HIV regulatory and accessory gene mRNAs.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:108 (poster no. 66). Unique Identifier : AIDSLINE ASHM5/94348926
Smith J; Penschow J; Deacon NJ; AIDS Molecular Biology Lab, Macfarlane Burnet Centre for Medical; Research, Fairfield, Vic.

Abstract: The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1 specific oligonucleotide primer pairs in a polymerase chain reaction procedure on cDNA reverse transcribed from cytoplasmic RNA extracted from lymphocytic cell lines infected with HIV-1 228200. The amplified products were analysed by hybridisation with spliced junction-specific oligonucleotide probes to determine splice donor/splice acceptor combination utilisation and the temporal expression of different mRNA species. This work has led to the identification of two novel splice acceptor sites which are used in the early production of a series of distinct RNAs upon acute infection. These RNAs have also been demonstrated in infected cells by in-situ hybridisation with the splice junction specific probes. Work is currently being carried out to determine the function of these RNAs.

Keywords: Cell Line, Transformed Genes, Regulator/*GENETICS Human HIV-1/*GENETICS In Situ Hybridization RNA Probes RNA Splicing/*GENETICS RNA, Messenger/*GENETICS ABSTRACT
941230
M94C4301

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