Induced expression of HIV-1 genomic RNA in latently infected cells followed tat, but preceded rev, mRNA expression. NLM AIDSLINE Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Induced expression of HIV-1 genomic RNA in latently infected cells followed tat, but preceded rev, mRNA expression.

Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:109 (poster no. 68). Unique Identifier : AIDSLINE ASHM5/94348928
Peng H; Haase AT; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.

Abstract: The kinetics of individual viral RNA synthesis of HIV-1 regulatory and structural genes in ACH2 cells after PMA induction were analysed at the single cell level using in situ hybridisation with splice-junction probes. Before PMA stimulation, only a small fraction (< 23%) of the cells expressed tat, nef, env or gag/genomic RNA, no cells expressed rev. One hour after induction, the percentage of tat, nef, env and gag/genomic RNA expressing cells increased to 85%, 99%, 49% and 74%, respectively, only 12% of the cells expressed rev mRNA. Seven hours after induction, 96% of the cells expressed tat mRNA; 100% expressed env and gag/genomic RNA; 38% expressed rev mRNA. Thereafter the expression level of env and gag/genomic RNA, but not tat and rev mRNA, on individual cells continued to increase. Our results showed that in the PMA-ACH-2 system, induced expression of HIV-I env and gag/genomic RNA followed the enhanced expression of tat and nef mRNA but preceded the induced expression of rev mRNA.
Keywords: Cell Line Gene Expression Regulation, Viral/PHYSIOLOGY Genes, env/GENETICS Genes, gag/GENETICS Genes, rev/*GENETICS Genes, tat/*GENETICS Human HIV-1/*GENETICS RNA, Messenger/*GENETICS RNA, Viral/*GENETICS Virus Latency/*GENETICS ABSTRACTKWDcelllinegeneexpressionregulation,viral/physiologygenes,env/geneticsgenes,gag/geneticsgenes,rev/KWDgeneticsgenes,tat/KWDgeneticshumanhiv-1/KWDgeneticsrna,messenger/KWDgeneticsrna,viral/KWDgeneticsviruslatency/KWDgeneticsabstract
941230
M94C4299

Copyright © 1994 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1994. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1994. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .