1994

Managing early HIV infection : AHCPR clinical practice guideline. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1994 1 videocassette (30 min.) : sd., col. ; 1/2 in. + 1 booklet (NCME telecourse ; no. 666) Unique Identifier : AIDSLINE AV/9441209; Oleske JM; A co-chair of the panel that developed the AHCPR HIV guideline discusses selected elements of adult and pediatric care. Topics include disclosure of HIV status, CD4 lymphocyte counts, prevention and treatment of opportunistic infections, antiretroviral therapy, and systems for case management.
[Bibliografia sobre los aspectos epidemiologicos, sociales, legales y eticos del SIDA.]: Rio Piedras, P.R. : Institute of Caribbean Studies, c1990 v, 338 p (Serie bibliografica ; num. 1 = Bibliographical series ; no. 1) Unique Identifier : AIDSLINE CAT/9413402; Cunningham I
[Igrejas e AIDS.]: Rio de Janeiro : ISER, 1990- v. ill Unique Identifier : AIDSLINE CAT/9407460
[Als je het nou van hard werken kreeg: : tien jaar AIDS en homocultuur.]: [Netherlands] : Veen, c1991 158 p. : ill (Homostudies Utrecht) Unique Identifier : AIDSLINE CAT/9403732; Kerkhof Mv
[AIDS e enfermagem obstetrica.]: Sao Paulo, Brasil : E.P.U., c1991 vii, 61 p. : ill Unique Identifier : AIDSLINE CAT/9408141; Marin Hd
[Le:depistage de l'infection par le VIH.]: [Paris : Le Haut Comite], c1992 iii, 45 p (Collection Avis et rapports du HCSP) Unique Identifier : AIDSLINE CAT/9415265
[AIDS und niedergelassene Arzte/Arztinnen in Wien : eine empirische Untersuchung zur ambulanten Versorgung von HIV-positiven und AIDSkranken Patient/innen.]: Wien : Institut fur Soziologie, Universitat Wien, Grund- und Integrativwissenschaftliche Fakultat, 1992 57 p (Schriftenreihe des Instituts fur Soziologie, Universitat Wien, Grund- und Integrativwissenschaftliche Fakultat ; 27) Unique Identifier : AIDSLINE CAT/9420416; Edlinger J
[Un:sang impur : l'affaire des hemophiles contamines.]: [S.l.] : J.C. Lattes, c1992 211 p Unique Identifier : AIDSLINE CAT/9412310; Paugam S
[Partikulare Arzneistofftrager als System fur Drug-Targeting : zur Therapie der HIV-Infektion und AIDS.]: Frankfurt/Main : VAS, c1993 155 p. : ill Unique Identifier : AIDSLINE CAT/9420513; Schafer V
AIDS and the heterosexual population.: Switzerland ; USA : Harwood Academic, c1993 xix, 281 p. : ill Unique Identifier : AIDSLINE CAT/9432615; Sherr L
The:Changing face of AIDS : implications for social work practice.: Westport, Conn. : Auburn House, 1993 xxix, 251 p. : ill Unique Identifier : AIDSLINE CAT/9431245; Lynch VJ; Lloyd GA; Fimbres M
[Migrants et perception du Sida : Le Maitre des infideles.]: Paris : L'Harmattan, c1993 143 p (Sante, societes et cultures) Unique Identifier : AIDSLINE CAT/9418253; Moumen-Marcoux R
[Netzwerk AIDS : 10 Jahre AIDS-Hilfe und ihre psychosozialen Angebote.]: Berlin : Deutsche AIDS-Hilfe, [1993] 175 p. : ill., ports Unique Identifier : AIDSLINE CAT/9423000; Biniasz T
Could it be HIV? : the clinical recognition of HIV infection.: Kingsgrove, NSW : Australasian Medical Pub. Co., 1993 76 p. : ill Unique Identifier : AIDSLINE CAT/9422520; Stewart G
[Dokumentation AIDS im Strafvollzug : Ergebnisse der Befragung von Menschen mit HIV/AIDS in bundesdeutschen Strafvollzugsanstalten.] 1. Aufl.: Berlin : Deutsche AIDS-Hilfe, [1993] 80 p. : port (AIDS Info) Unique Identifier : AIDSLINE CAT/9422739; Gahner M
HIV prevention.: Austin, TX : The Bureau, v Unique Identifier : AIDSLINE CAT/9432578
[Rechtliche Bedeutung des Status HIV-positiv : Leitfaden fur Sozialversicherungsrecht, Privatversicherungsrecht, Arbeitsrecht und Strafrecht.]: Basel : Verlag Helbing & Lichtenhahn, c1993 98 p Unique Identifier : AIDSLINE CAT/9420539; Keller M
AIDS : crisis in professional ethics.: Philadelphia : Temple University Press, 1994 vi, 276 p Unique Identifier : AIDSLINE CAT/9433456; Cohen ED; Davis M
AIDS, ethics & religion : embracing a world of suffering.: Maryknoll, N.Y. : Orbis Books, c1994 ix, 284 p Unique Identifier : AIDSLINE CAT/9433075; Overberg KR
HIV, perinatal infections, and therapy : the role of the placenta.: Rochester, N.Y. : University of Rochester Press, 1994 xvii, 614 p. : ill., port (Trophoblast research ; v. 8) Unique Identifier : AIDSLINE CAT/9441204; Miller RK; Thiede HA
H-2K(b) gene and retrovirus interactions in the regulation of BL6 melanoma cell sensitivity to tumor necrosis factor-alpha.: Diss Abstr Int [B]; 54(6):2980 1993. Unique Identifier : AIDSLINE ICDB/94605777; Kim M; Univ. of Pittsburgh; The effect of major histocompatibility complex (MHC) class I gene expression on the sensitivity of BL6 melanoma cells to the cytotoxic effects of TNF-alpha was studied. Experiments were performed using B16F10BL6 melanoma line (hereafter referred as BL6) that is a highly invasive and metastatic cell line. BL6 melanoma
Adoptive immunotherapy of cancer: a new approach for cancer treatment.: Diss Abstr Int [B]; 54(6):2983 1993. Unique Identifier : AIDSLINE ICDB/94605782; Qin YC; Limburgs Universitair Centrum, Belgium; The conventional treatments of cancer are surgery, chemotherapy and radiotherapy. However, the therapeutic efficacy of these treatments are still far from satisfactory in most cases. With the development of immunology and increasing understanding in tumor immunology, immunotherapy has moved onto the stage of cancer tr
The endothelium in HIV-associated Kaposi's sarcoma.: Diss Abstr Int [B]; 54(6):3004 1993. Unique Identifier : AIDSLINE ICDB/94605793; Corbeil J; Univ. of New South Wales, Australia; The aim of this project was to culture Kaposi s sarcoma-derived (KS) cells in order to investigate its etiology and attempt to elucidate the role of the vascular endothelial cell (EC) in this pathological process. The establishment of five cell cultures derived from pleural or peritoneal fluid of HIV-infected individu
Interleukin-6 and the lung.: Lung Biol Health Dis; 61:229-80 1993. Unique Identifier : AIDSLINE ICDB/94606665; Zitnik RJ; Elias JA; Dept. of Internal Medicine, Yale Univ. Sch. of Medicine, New; Haven, CT; Interleukin-6 (IL6) is a pleiotropic cytokine produced by a wide variety of cells, including many of the major cells in the lung, and in response to a broad spectrum of stimuli (eg, viral infection, bacterial endotoxin, agonists of cAMP, PKC, and calcium 2nd-messenger systems). Regulation of IL6 production is complex
Retroviruses in human cancer (Meeting abstract).: EACR-12: 12th Biennial Meeting of the European Association for Cancer Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier : AIDSLINE ICDB/94697506; Weiss RA; Chester Beatty Laboratories, Inst. of Cancer Res., 237 Fulham; Rd., London SW3 6JB, UK; The retroviruses linked with human cancer fall into two distinct categories. Human T-cell leukemia viruses (HTLV-I, HTLV-II) are oncoviruses. HTLV-I infection accounts for the major burden of adult T-cell lymphoma and leukemia in regions such as Japan , the Caribbean basin and some W
Subtypes of Epstein-Barr virus in HIV-1-positive and HIV-1-negative patients with Hodgkin's disease (Meeting abstract).: EACR-12: 12th Biennial Meeting of the European Association for Cancer Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier : AIDSLINE ICDB/94697510; De Re V; De Vita S; Marzotto A; Gloghini A; Carbone A; Uccini S; Scarpa A; and Boiocchi M; Div. of Experimental Oncology, Aviano, Italy; Considerable evidence suggests that EBV has a role in the pathogenesis of Hodgkin s disease (HD). In immunocompetent hosts type 2 EBV is considered to be a much less potent transformer of lymphocytes than type 1 virus strain. However, type 2 EBV may be involved in the pathogenesis of non-Hodgkin s lymphomas arising in
Bispecific antibodies recruiting cellular effectors (Meeting abstract).: EACR-12: 12th Biennial Meeting of the European Association for Cancer Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier : AIDSLINE ICDB/94697527; Thielemans K; Lab. of Physiology-Immunology, Vrije Universiteit Brussel, B-1090; Brussels, Belgium; The idea to target T lymphocytes toward tumor cells has been tested extensively and successfully by many laboratories. Our laboratory has evaluated this attractive approach in vivo. We have developed bispecific antibodies by hybrid-hybridoma technique, with dual specificity: the murine CD3 complex and the idiotype (tu
Reconstitution of viral immunity by the adoptive transfer of T-cell clones modified by gene insertion (Meeting abstract).: EACR-12: 12th Biennial Meeting of the European Association for Cancer Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier : AIDSLINE ICDB/94697579; Greenberg P; Watanabe K; Gilbert M; Nelson B; Riddell S; Univ. of Washington, Seattle, WA 98195; The essential role of CD8+ Tc for protection from CMV disease is supported by studies in which we demonstrated that patients who endogenously reconstitute their CMV-specific CD8+ Tc response are protected from the development of CMV disease, whereas patients lac
Molecular Biology of Hematopoiesis, 8th Symposium. July 9-13, 1993, Basel, Switzerland.: Molecular Biology of Hematopoiesis, 8th Symposium. July 9-13, 1993, Basel, Switzerland, 222 p., 1993.. Unique Identifier : AIDSLINE ICDB/94699269; Anonymous; No affiliation given; The 8th symposium on Molecular Biology of Hematopoiesis, held July 9-13, 1993, in Basel, Switzerland , addressed these topics: bone marrow transplantation, immune stimulation and suppression, development of T and B cells, viruses and cytokines, HIV and other viruses, gene regul
Genetically modified T-cell clones as a treatment for human viral diseases (Meeting abstract).: SBT93: Society for Biological Therapy, 8th Annual Scientific Meeting: Biological Therapy of Cancer--VIII. November 10-14, 1993, Nashville, TN, p. 50, 1993.. Unique Identifier : AIDSLINE ICDB/94699586; Riddell SR; Overell RW; Lupton S; Greenberg PD; Fred Hutchinson Cancer Res. Center, 1124 Columbia St., Seattle,; WA 98104; The adoptive transfer of MHC restricted antigen-specific T cells is an effective therapy for viral diseases and malignancies in animal models. Recent advances in the in vitro isolation and expansion of human antigen-specific T cell clones have facilitated the development of clinical trials to define the principles for
Treatment with zidovudine (AZT) is not associated with increased B cell activation in HIV infection (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1137 1994. Unique Identifier : AIDSLINE ICDB/94602823; Widney D; Vander Meyden M; Martinez-Maza O; Dept. of Microbiology and Immunology, UCLA Sch. of Medicine, Los; Angeles, CA 90024; An increased frequency of AIDS-associated lymphoma has been reported to be associated with AZT treatment. B cell hyperactivation, which is often seen in HIV infection, is thought to contribute directly to the development of lymphoma. To determine if A
Liposome encapsulated doxorubicin for the treatment of AIDS-associated Kaposi's sarcoma (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1139 1994. Unique Identifier : AIDSLINE ICDB/94602825; Harrison M; Coker R; Tomlinson D; Stewart S; Dept. of Oncology, St Mary's Hosp., London W2 1NY, UK; Severe KS requiring chemotherapy affects 20-30% of patients with AIDS. Doxorubicin within a polyethylene glycol containing liposome (Doxil, LTI) has been used in a phase 2 study on 34 patients with severe KS. The liposome is 90 nm in diameter and is composed of 20% cholesterol, 20% polyethylene glycol, and 60% phospha
Pediatric HIV associated non-Hodgkin's lymphomas (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1140 1994. Unique Identifier : AIDSLINE ICDB/94602826; Shad A; Bhatia K; Hamdy N; Mueller B; Pizzo P; Whang Peng J; Jaffe E; Magrath I; Pediatric Branch, Pathology Dept., NCI, Bethesda, MD 20892; HIV infected children, like HIV infected adults, are at increased risk for development of non-Hodgkin s lymphomas (NHL). However, there is very little information available on the histological, cytogenetic and molecular features of pediatric HIV associated NHLs. We present here the pathological and biological features
HIV lymphomas frequently carry mutations in the c-myc protein (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1141 1994. Unique Identifier : AIDSLINE ICDB/94602827; Spangler G; Gaidano G; Hamdy N; Dalla-Favera R; Magrath I; Bhatia K; Pediatric Branch, NCI, Bethesda, MD 20892; Non-Hodgkin s lymphomas, particularly high grade B cell lymphomas, are the most frequent malignancies diagnosed in patients infected with HIV. We have previously reported the occurrence of point mutations in the protein coding region of c-myc in approximately 60% of SNCL in non-HIV infected patients. We have now scree
Proliferation of leiomyosarcoma cell lines is inhibited in vitro by antisense oligonucleotides directed against the insulin growth factor Type I receptor (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1543 1994. Unique Identifier : AIDSLINE ICDB/94603229; Hirschfeld S; Helman LJ; Pediatric Branch, NCI, Bethesda, MD; Smooth muscle proliferation can lead to a variety of pathologic conditions, including benign (leiomyoma) and malignant (leiomyosarcoma; LMS). Published studies have shown that smooth muscle cell proliferation can be enhanced by insulin growth factor I (IGF-I), and that surgical specimens of LMS contain RNA transcripts
Hyperstabilization of sense-antisense duplexes and inhibition of translation (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1835 1994. Unique Identifier : AIDSLINE ICDB/94603521; Kim DY; Shih DS; Cho DY; Swenson DH; School of Veterinary Medicine, Louisiana State University, Baton; Rouge, LA 70803; The antitumor agents CC-1065 and U-71,184 raise the Tm of double-stranded DNA. We undertook this study to determine if these agents could hyperstabilize RNA-DNA (sense-antisense) duplexes or DNA duplexes in which one strand contained phosphorothioate (PS) or methylphosphonate (MP) internucleotide linkages. Sequences 1
Mechanism of disproportionate antiviral activity of 2',3'-dideoxynucleoside analogs and their classification (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1841 1994. Unique Identifier : AIDSLINE ICDB/94603527; Gao WY; Agbaria R; Driscoll JS; Mitsuya H; NCI, Bethesda, MD 20892; The mechanism of disproportionate anti-HIV-1 activity of 2 ,3 -dideoxynucleoside (ddN) in resting and activated target cells was studied. The in vitro activity of various ddNs against human immunodeficiency type 1 (HIV-1) profoundly differed from each other. The divergent antiviral activity was associated with differe
Increased survival of immunodeficient retrovirus infected mice treated with lithium (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1824 1994. Unique Identifier : AIDSLINE ICDB/94603528; Gallicchio VS; Cibull ML; Hughes NK; Tse KF; Hematology and Oncology Division, Department of Internal; Medicine, University of Kentucky Medical Center, Lexington, KY; 40536; Murine immunodeficiency virus disease (MAIDS) induced with LP-BM5 MuLV shows many similarities to human HIV-infection. The etiological agent is a defective MuLV capable of inducing disease with the aid of a helper virus. Lithium (Li) influences numerous immunohematopoietic cell types and cellular processes that involv
Analysis of peripheral lymphocytes of lymphoma patients for daunomycin (DNR) retention and cell differentiation antigens (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1846 1994. Unique Identifier : AIDSLINE ICDB/94603532; Samy TS; Byrnes J; Krishan A; Harrington WJ Jr; Byrnes G; Wellham LL; Sauerteig A; Fletcher MA; University of Miami School of Medicine, Miami, FL 33136; The lymph node cells and peripheral blood lymphocytes of lymphoma patients were analyzed for cellular DNR retention, response to efflux blockers and expression of human differentiation antigens. Extensive heterogeneity in cellular DNR retention was seen in lymphocytes in 10/66 patients. Cellular DNR retention could be
Synthesis and antiviral evaluation of 2',3'-dideoxy-L-pyrimidine nucleosides with an improved in vitro therapeutic index against hepatitis B and human immunodeficiency virus (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1848 1994. Unique Identifier : AIDSLINE ICDB/94603534; Lin TS; Luo MZ; Liu MC; Pai SB; Dutschman GE; Cheng YC; Dept. of Pharmacology, Yale Univ. School of Medicine, New Haven,; CT 06510; The limiting toxicity of the clinically approved antiviral drug 2 ,3 -dideoxycytidine (beta-D- ddC ) is peripheral neuropathy , which may be caused by the inhibition of mitochondrial DNA synthesis. Vario
Protective effects of swainsonine against toxicity of chemotherapeutic drugs (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1909 1994. Unique Identifier : AIDSLINE ICDB/94603595; Klein JL; George MD; Roberts JD; Kurtzberg J; Breton P; Chermann JC; Olden K; Lab of Molecular Carcinogenesis, NIEHS, Research Triangle Park,; NC 27709; Swainsonine, a plant-derived alkaloid, abrogates the cytotoxicity of drugs commonly used in cancer and AIDS therapy. In a murine bone marrow progenitor colony-forming assay, as previously published, CFU-E were more sensitive to AZT toxicity than were
Edema induced by factors released by AIDS-associated Kaposi's sarcoma cells (AIDS-KS cell) (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A402 1994. Unique Identifier : AIDSLINE ICDB/94602090; Nakamura S; Murakami-Mori K; Sakurada S; Rajeeve S; Rao N; Weich H; Salahuddin SZ; Inst. of Molecular Medicine and Technology, Pasadena, CA 91105; Angiogenesis and in some cases edema are two major components of AIDS-KS. Cultured spindle cells isolated from various KS lesions of patients with AIDS transplanted in nude mice induce KS-like lesions of murine origin. The critical factor for this purpose is oncostatin H. We report here that AIDS-KS cells also release
Replication inhibition and miscoding properties of a DNA template containing N-(deoxyguanosin-8-yl)-1-aminopyrene (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A854 1994. Unique Identifier : AIDSLINE ICDB/94602541; Vyas RR; Basu AK; Dept. of Chemistry, Univ. of Connecticut, Storrs, CT 06269; A major DNA adduct formed by the environmental pollutant 1-nitropyrene is N-(deoxyguanosin-8-yl)-1-aminopyrene (dG(AP)). We have synthesized a DNA fragment that contained this adduct at a specific site. A primer was annealed to this template and in vitro DNA synthesis by a variety of polymerases was studied. Primer ex
Growth suppression and toxicity induced by caffeic acid phenethyl ester (CAPE) in type 5 adenovirus transformed rat embryo cells correlates directly with transformation progression (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A979 1994. Unique Identifier : AIDSLINE ICDB/94602665; Su ZZ; Lin J; Grunberger D; Fisher PB; Columbia University, College of Physicians and Surgeons, New; York, NY 10032; The active component of the honeybee hive product propolis, CAPE, induces a selective growth suppressive and toxic effect toward cloned rat embryo fibroblast (CREF) cells transformed by adenovirus type 5 (Ad5) or the Ad5 E1A transforming gene versus untransformed CREF cells. The present study was conducted to determin
Chemotherapy associated with zidovudine and Gm-CSF in human immunodeficiency virus-related non-Hodgkin's lymphoma (HIV-NHL) (Meeting abstract).: Proc Annu Meet Am Assoc Cancer Res; 35:A1138 1994. Unique Identifier : AIDSLINE ICDB/94602824; Gabarre J; Lepage E; Thyss A; Bastion Y; Sebban C; Schlaifer D; Ribrag V; Fereres M; Raphael M; Gisselbrecht C; GELA, Saint-Louis Hosp., Paris, France; HIV-NHL patients have an estimated survival according to authors between 5-9 months, a better survival can be obtained in patients with a good performance status (PS), CD4 count greater than 100 ul/mm3 and without AIDS. Main causes of death are lymphoma, opportunistic infections and therapy toxicity. In order to reduc
Analysis of strand transfer synthesis by reverse transcriptases.: Diss Abstr Int [B]; 54(7):3597 1994. Unique Identifier : AIDSLINE ICDB/94605826; Buiser RG; Univ. of Rochester; Models of retrovirus replication require the viral reverse transcriptase (RT) to catalyze two distinct strand transfer reactions. In this process, an RNA primer is elongated on one template and then transferred to another template for additional elongation. It is thought that the RT ribonuclease H (RNase H) activity i
Lack of inhibition of HIV replication in chronically infected macrophages by Tat inhibitors Ro-5-3335 and Ro-24-7429.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:100 (poster no. 51). Unique Identifier : AIDSLINE ASHM5/94348910; Dunne A; Siregar H; Sonza S; Crowe S; Macfarlane Burnet Centre for Medical Research, Fairfield; Hospital, Vic.; OBJECTIVE: HIV infects different cell types including CD4+ lymphocytes and monocyte/macrophages (Mphi). We have examined the effect of the Tat inhibitors Ro-5-3335 and Ro-24-7429 in cultured human peripheral Mphi. METHODS: MO were isolated from HIV seronegative donors by gradient centrifugation and plastic adherence.
Optimisation of microculture methods for quantitation of HIV from plasma and cellular fractions of peripheral blood.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:100 (poster no. 50). Unique Identifier : AIDSLINE ASHM5/94348911; Dunne A; Crowe S; Macfarlane Burnet Centre for Medical Research, Fairfield; Hospital, Victoria.; OBJECTIVE: Quantitation of HIV in plasma and PBMCs is used in clinical trials to follow disease progression and determine drug efficacy. We have modified the ACTG protocols to reduce cost and optimise virus isolation. METHODS: Microculture procedures as described by the AIDS Clinical Trials Group (ACTG) have been used
Lymphocyte subset analysis by Boolean algebra: a phenotypic approach using a cocktail of 5 antibodies and 3 colour immunofluorescence.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 52). Unique Identifier : AIDSLINE ASHM5/94348912; Hunter S; Peters L; Wotherspoon J; Crowe S; Flow Cytometry Unit, Macfarlane Burnet Centre for Medical; Research, Fairfield Hospital, Victoria, Australia.; A rapid method has been developed whereby total CD3+ T-cells, CD4+ T-cells (CD3+CD4+), CD8+ T-cells (CD3+CD8+), putative gamma delta-receptor-T-cells (CD3+CD4-CD8-) and T-cells that are CD3+CD4+CD8+ as well as B-lymphocytes and NK-cells can be enumerated after staining in a single tube. Whole blood specimens are label
Mechanisms of AZT resistance in HIV isolates.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 53). Unique Identifier : AIDSLINE ASHM5/94348913; Buchanan L; Tachedjian G; Birch C; Virology Department, Fairfield Hospital, Victoria.; AIM: To investigate the susceptibility of the reverse transcriptase (RT) and ribonuclease H (RNase H) from AZT-sensitive (AZT-S) and AZT-resistant (AZT-R) HIV isolates to AZT-triphosphate (AZTTP) and AZT-monophosphate (AZTMP). METHODS: Isolates obtained from a patient before and after long-term AZT therapy were examin
Characterisation of putative steroid response elements in the long terminal repeat of HIV-1.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:102 (poster no. 55). Unique Identifier : AIDSLINE ASHM5/94348914; Barnes N; Deacon N; Doherty R; Macfarlane Burnet Centre, Fairfield, Vic.; Motifs with sequence homology to steroid hormone response elements have been identified in the upstream half of the LTR of HIV by several investigators, and nuclear factors have been identified in HeLa cell extracts which bind to these regions. The motifs appear conserved in HIV consensus sequences, and to a variable
Mechanism of action of Coumermycin A1.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:102 (poster no. 54). Unique Identifier : AIDSLINE ASHM5/94348915; Jardine D; Locarnini S; Tachedjian G; Birch C; Virology Dept., Fairfield Hospital, Victoria.; AIM: To investigate the mechanism of action of Coumermycin A1 against HIV. METHODS: We investigated the in vitro anti HIV activity of coumermycin A1 in acutely infected PBLs, T-cell lines (MT-2) and macrophages and in chronically infected cells (H9-HTLVIIIB and macrophages). Several in vitro assays, such as reverse tr
Characterisation of HIV-1 LTR DNA-binding proteins.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 57). Unique Identifier : AIDSLINE ASHM5/94348916; Peters A; Deacon NJ; AIDS Molecular Biology Laboratory, Macfarlane Burnet Centre for; Medical Research, Fairfield, Vic., Australia.; An investigation into the characterisation of mammalian nuclear proteins which bind to the HIV-1 LTR U3 region has been carried out using a series of overlapping 40-mer oligonucleotides. These oligonucleotides were synthesised to correspond to the HXB2 sequence from n.t. -210 to n.t. -454. Two oligonucleotides have sh
Role the immunodeficiency virus nef gene and the production in CNS tropism.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 56). Unique Identifier : AIDSLINE ASHM5/94348917; Smith K; Deacon NJ; McPhee DA; Brew B; AIDS Molecular Biology Unit, Macfarlane Burnet Centre, Vic.,; Australia.; Particular strains of HIV may dominate the CNS of infected individuals and it is possible that the HIV Nef protein plays a role in differentiating CNS and peripheral blood isolates. To address this, 6 pairs of coincident isolates from the CSF and peripheral blood of patients with AIDS or ARC were analysed for their ab
Cytokine dysregulation associated with the course of HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:104 (poster no. 58). Unique Identifier : AIDSLINE ASHM5/94348918; Fan J; Bass H; Nguyen T; Fahey JL; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.; By using quantitative RT-PCR, elevated levels of IFN gamma mRNA and lowered IL-2 mRNA were found in PBMC of HIV-seropositive men with CD4 T cells over 500/mm3 (mean:647/mm3) while IL-4 and IL-10 mRNAs did not change significantly. Men whose CD4 levels fell more than 20
Detection of HIV-1 tat, rev and nef mRNA at single cell level.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:104 (poster no. 59). Unique Identifier : AIDSLINE ASHM5/94348919; Peng H; Haase AT; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.; We have developed a strategy of in situ hybridisation using splice-junction probes which can specifically detect HIV-1 regulatory and structural gene mRNAs. This approach takes advantage of the fact that although tat, rev and nef share the same 5 splice donor, they differ in their use of the first splice acceptors. E
Primary T-cell response to HIV-1 in vitro.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:105 (poster no. 60). Unique Identifier : AIDSLINE ASHM5/94348920; Todd B; Pope J; NCHVR, Royal Children's Hospital, Brisbane, Qld.; HIV-I-specific, MHC-restricted cytotoxic T cells may be detected directly in peripheral blood (PB) of HIV-1-infected patients, and cytotoxic T-cell lines can be established in vitro. CD8+ T cells predominate, and epitopes exist in most viral antigens. Generation of cytotoxic T cells from the PB mononuclear cells from
Transcription factors in HIV-1 infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:105 (poster no. 61). Unique Identifier : AIDSLINE ASHM5/94348921; Kedar P; Arden KE; Todd BJ; Pope JH; NCHVR, Royal Children's Hospital, Brisbane, Qld.; Transcriptional regulation involves the interaction of enhancer-promoter regions with cellular binding proteins. NF-kB is a nuclear transcriptional factor which binds and mediates transcription from the sequences in the promoter region. In our system we have studied NF-kB and related proteins in PHA stimulated cord bl
CD4-bearing human thymic dendritic cells as potential targets for HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:106 (poster no. 63). Unique Identifier : AIDSLINE ASHM5/94348922; Sotzik F; Shortman K; Walter and Eliza Hall Institute of Medical Research, Royal; Melbourne Hospital, Parkville, Victoria.; The intention of the project was the isolation of CD4-bearing T-precursor thymocytes. This laboratory has found these to be the earliest T-precursors in the murine thymus with T cell receptor genes in the germline, unrearranged state. In order to identify the human equivalent, CD34 and CD7 expression has been examined
HIV strains and the proliferative response of mononuclear cells from normal donors.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:106 (poster no. 62). Unique Identifier : AIDSLINE ASHM5/94348923; Adams S; Todd B; Pope J; NCHVR, Royal Children's Hospital, Brisbane, Qld.; Recent interest has focused on the different biological properties of HIV-1 isolates. Non-syncytium-inducing (NSI) isolates, detectable at all stages of HIV infection, are preferentially monocytotropic in contrast to SI isolates which precede symptoms of AIDS and are T-cell tropic. We are examining the effects of NSI
DdI susceptibility of HIV-1 isolates in patients on long-term therapy.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:107 (poster no. 64). Unique Identifier : AIDSLINE ASHM5/94348924; Land S; McGavin C; Corby S; Tyssen D; Gorry P; Magliano D; Birch C; State HIV Isolation Laboratory, Virology Department, Fairfield; Hospital, Victoria.; Sequential HIV isolates obtained from 18 individuals receiving dideoxyinosine (ddI) were tested for susceptibility to the drug. All patients had received AZI before commencing ddI therapy for up to 18 months. Susceptibility assays were performed in peripheral blood lymphocytes infected with these isolates and maintain
Detection of HIV-1(HTLV-I) pseudotypes.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:107 (poster no. 65). Unique Identifier : AIDSLINE ASHM5/94348925; Lawson V; Doultree J; Lee JY; Marshall J; McPhee D; Macfarlane Burnet Centre for Medical Research, NCHVR, Fairfield,; Vic.; The mixing of envelope glycoproteins in cells infected with two viruses may result in the formation of pseudotypes. Several studies have indicated that human immunodeficiency virus (HIV) may form pseudotypes with several viral species including human T-cell leukemia virus (HTLV-I), murine amphotropic virus and HIV-2.
HIV regulatory and accessory gene mRNAs.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:108 (poster no. 66). Unique Identifier : AIDSLINE ASHM5/94348926; Smith J; Penschow J; Deacon NJ; AIDS Molecular Biology Lab, Macfarlane Burnet Centre for Medical; Research, Fairfield, Vic.; The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1 specific oligonucleotide primer pairs in a polymerase chain reaction procedure on cDNA reverse transcribed from cytoplasmic RNA extracted from lymphocytic cell lin
The study of Nef mutant clones of human immunodeficiency virus (HIV).: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:108 (poster no. 67). Unique Identifier : AIDSLINE ASHM5/94348927; Arunagiri CK; Peden K; Azad A; McPhee D; Biomolecular Research Institute, Parkville.; A study was initiated to determine the function of Nef auxiliary protein replication of HIV. A molecular clone of HIV, LAI, was selected and two truncated (termination at amino acid 46 and 88) nef mutant clones constructed. The proviral DNA of the mutants and wild type were used to transfect Hela cells. Virus produced
Induced expression of HIV-1 genomic RNA in latently infected cells followed tat, but preceded rev, mRNA expression.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:109 (poster no. 68). Unique Identifier : AIDSLINE ASHM5/94348928; Peng H; Haase AT; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.; The kinetics of individual viral RNA synthesis of HIV-1 regulatory and structural genes in ACH2 cells after PMA induction were analysed at the single cell level using in situ hybridisation with splice-junction probes. Before PMA stimulation, only a small fraction (
PCR analysis for common HIV-1 zidovudine resistance mutations in pol DNA of untreated and zidovudine treated patients.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:109 (poster no. 69). Unique Identifier : AIDSLINE ASHM5/94348929; Salem H; Nightingale B; Garsia RJ; Molecular Biology Department, Royal Prince Alfred Hospital,; Camperdown, NSW.; METHODS: Mutations of the HIV-1 pol gene, causing amino acid changes at codons 41, 67, 70, 215 and 219, were sought in DNA of serial HIV-1 infected patients. Exposure to Zidovudine (ZDV) was nil in 12 cases and ranged from 36 months in the remaining 55 samples. The PCR amplified two regions of pol encom
Mechanisms of AZT resistance in HIV isolates.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:110 (poster no. 70). Unique Identifier : AIDSLINE ASHM5/94348930; Buchanan L; Tachedjian G; Birch C; NCHVR, Fairfield Hospital, Victoria.; To investigate the susceptibility of the reverse transcriptase (RT) and ribonuclease H (RNase H) from AZT-sensitive (AZT-S) and AZT-resistant (AZT-R) HIV isolates to AZT-triphosphate (AZTTP) and AZT-monophosphate (AZTMP). METHODS--Isolates obtained from a patient before and after long-term AZT therapy were examined fo
In-field assay monitoring of anti-HIV-1/HIV-2 EIAs in Australian Blood Banks, 1992. Australian Red Cross Blood Transfusion Service Laboratories.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:111 (poster no. 72). Unique Identifier : AIDSLINE ASHM5/94348931; Dax EM; Bendistinto M; Vandenbelt T; National HIV Reference Laboratory (NRL), Fairfield Hospital,; Victoria, Australia.; OBJECTIVE: To examine the performance of 4 anti-HIV-1/HIV-2 enzyme immunoassays (EIAs) in Australian Blood Banks during use. EIAs included: Abbott Recombinant, Diagnostic Pasteur Genelavia MIXT, Genetic Systems EIA and Organon Teknika. METHODS: Anti-HIV-1/HIV-2 EIAs were monitored by collecting data from individual la
Modelling the HIV-1 infection cycle.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:112 (poster no. 73). Unique Identifier : AIDSLINE ASHM5/94348932; Deacon N; McPhee D; Newnham J; Linger H; AIDS Molecular Biology Unit, Monash University.; We have developed a computational tool for biological research to study the replication strategy of the human immunodeficiency virus. This model incorporates a formal model using Petri Nets and scientific citations. In the development of the formal model we have uncovered some facets of the replication strategy that w
Management of HIV disease.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:18 (abstract no. TPI-1). Unique Identifier : AIDSLINE ASHM5/94348933; Volberding P; San Francisco General Hospital.; Appropriate clinical care of the HIV-infected patient includes consideration of antiretroviral therapy, opportunistic infection, management, and the treatment of HIV-associated malignancies. In addition, the system of care must address the numerous psychosocial issues posed by progressive fatal disease, typically occu
Antiretroviral therapy of HIV: a virologists perspective.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:19 (abstract no. TPI-4). Unique Identifier : AIDSLINE ASHM5/94348934; Corey L; University of Washington, Seattle.; While the availability of nucleoside reverse transcriptase (RT) inhibitors have revolutionised care of HIV infected persons, controversy exists about the overall utility of these medications. When should therapy be initiated? With what agent? When should alternative therapy be utilised, what alternative should be give
Contamination of blood by HIV in France: facts, controversies and impact on society.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:19 (abstract no. TPI-3). Unique Identifier : AIDSLINE ASHM5/94348935; Brunet JB; European Centre for the Epidemiology Monitoring of AIDS,; Saint-Maurice, France.; Screening of blood donors has been mandatory in France since 1st August 1985. Virus inactivated Factor VIII concentrates were progressively introduced for treatment of haemophiliacs and by 1st October 1985 all non-heated products had been replaced. By 30th June 1993, 1223 AIDS case
Unravelling the molecular biology of HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:20 (abstract no. TPI-5). Unique Identifier : AIDSLINE ASHM5/94348936; Rosenberg M; SmithKline Beecham Pharmaceuticals, King of Prussia, PA; 19406-0939.; HIV is a genetically complex retrovirus which can integrate into the genome of its human host and thereby remain hidden from immune surveillance or replicate slowly to achieve its chronic infection process. The advent of the recombinant DNA technologies have allowed identification, molecular dissection and characteris
Ian Thompson Memorial Lecture. Opportunistic infections in people with HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:21 (abstract no. TPI-6). Unique Identifier : AIDSLINE ASHM5/94348937; van der Horst C; University of North Carolina.; In the last 10 years of the HIV epidemic therapeutic advances on the frontier of opportunistic infections have done more to prolong the quality and length of life for affected people than anti-retrovirals. Pneumocystis carinii pneumonia is less often seen due to widespread prophylaxis with trimethoprim-sulfamethoxazol
Drug resistance and Sl phenotype; implications for chemotherapy.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:22 (abstract no. FPI-2). Unique Identifier : AIDSLINE ASHM5/94348938; Richman DD; University of California San Diego 92093-0679.; The clinical and therapeutic significance of the emergence of diminished drug susceptibility has been difficult to elucidate. Two recent studies, one with nucleosides and one with a non-nucleoside reverse transcriptase inhibitor (NNRTI) address this issue. A multicenter virologic analysis of ACTG 116B/117 documented t
HIV vaccines.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:22 (abstract no. FPI-1). Unique Identifier : AIDSLINE ASHM5/94348939; Corey L; McElrath J; Kent S; Greenberg P; University of Washington, Seattle.; To date the NIAID sponsored AIDS Vaccine Evaluation Group (AVEU) has enrolled over 1000 patients into Phase I/II clinical trials of candidate HIV vaccines. 8 different envelope products have been evaluated including two gp160 preparations, two subunit gp120 products, one yeast derived gp120, one vaccinia gp160, 1 cana
Clinical management of HIV-related malignancies.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:23 (abstract no. FPI-3). Unique Identifier : AIDSLINE ASHM5/94348940; Volberding P; San Francisco General Hospital.; Two cancers are recognised as occurring in a higher rate in patients with HIV infection. These include non-Hodgkin s B cell lymphomas and Kaposi s sarcoma. In addition, some data indicates that Hodgkin s disease may also occur at an increased incidence. Many other malignancies have been reported in patients infected w
HIV related dementia.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:23 (abstract no. FPI-4). Unique Identifier : AIDSLINE ASHM5/94348941; Strain J; This paper will address the prevalence, the etiology, and the treatment of the organic mental disorders that accompany HIV infection, with special attention to the dementias. Late-stage HIV infection is frequently associated with a syndrome of progressive dementia, presumably etiologically related to the virus activit
Cytomegalovirus infections in people with HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:24 (abstract no. SPI-2). Unique Identifier : AIDSLINE ASHM5/94348942; van der Horst C; University of North Carolina.; By the time of death up to 40% of HIV infected people will develop life or sight threatening CMV infections. With some rare exceptions most of these infections will occur when the CD4 count drops below 100 cells/mm3 and usually do not occur until the CD4 count i
Update of HIV drug resistance.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:24 (abstract no. SPI-1). Unique Identifier : AIDSLINE ASHM5/94348943; Larder B; Antiviral Therapeutic Research Unit, Wellcome Research; Laboratories, U.K.; Initial reports that human immunodeficiency virus (HIV) isolates from individuals with advanced disease can become resistant to AZT after prolonged therapy precipitated considerable interest in the study of HIV drug resistance. Genetic analysis of the
Molecular targets for HIV therapy.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:25 (abstract no. SPI-3). Unique Identifier : AIDSLINE ASHM5/94348944; Rosenberg M; SmithKline Beecham Pharmaceutical, King of Prussia, Pennsylvania; 19406-0939.; There continues to be an urgent need for the discovery and design of better drugs with antiviral activities against the HIV viruses. The various stages of the HIV life cycle provide specific molecular targets for which novel assays and mechanistic approaches can be developed to search for and identify new inhibitory a
An unusual cause of nodular pulmonary opacities in HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:26 (abstract no. TC-2). Unique Identifier : AIDSLINE ASHM5/94348945; Lewin SR; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; Nodular pulmonary infiltrates are a difficult diagnostic problem in HIV infection. Various modalities of investigation are available to the clinician including bronchoscopy, CT guided and open lung biopsy as well as nuclear medicine studies. Infrequently, no diagnosis may be made. A 37 year old man with multiple AIDS
Pneumonitis in association with primary HIV illness.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:26 (abstract no. TC-1). Unique Identifier : AIDSLINE ASHM5/94348946; Kelly M; Lloyd A; Jones P; Department of Infectious Diseases, Prince Henry Hospital, Little; Bay, Sydney.; A 27 year old homosexual male presented initially with a 5 day illness characterised by fever and rash. Apart from a maculopapular rash there was no physical abnormality. The symptoms resolved spontaneously over a four day period. At presentation P24Ag was positive and then became negative whereas tests for HIV antibo
A cavitating lung infection followed by cytomegalovirus polyradiculopathy--discussion of two problems.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:27 (abstract no. TC-4). Unique Identifier : AIDSLINE ASHM5/94348947; Empson M; Britton W; Garsia R; Department of Clinical Immunology, Royal Prince Alfred Hospital,; Camperdown, NSW.; A 29 year old HIV positive man presented with pleuritic chest pain. haemoptysis and epistaxis. Investigations revealed a cavitating lung lesion, high titre cANCA and scanty pneumocysts. The course was complicated by clinical hypoadrenalism, secondary infection and delirium. CSF obtained to investigate the latter grew
Case presentation: a 35 year old man with cystic pneumocystis pneumonia, cerebro-ocular lymphoma and recurrent bacteraemia.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:27 (abstract no. TC-3). Unique Identifier : AIDSLINE ASHM5/94348948; Paterson DL; Allworth AM; Hospital Brisbane, Australia.; A 35 year old man HIV antibody positive since 1986 presented in February 1993 with pleuritic chest pain haemoptysis and dyspnoea. His past history included syphilis, bacillary angiomatosis , Kaposi s sarcoma and CMV reti
HIV related chronic reactive/suppurative airway disease: is CMV important?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:28 (abstract no. TC5). Unique Identifier : AIDSLINE ASHM5/94348949; Parkin D; Pigott P; Dept. of HIV Medicine, Royal North Shore Hospital, St Leonards,; NSW.; We describe a case of HIV related chronic reactive/suppurative airway disease in a non smoking 47 year old man. He had longstanding HIV infection treated with sporadic AZT . His initial complication was Pneumocystis carinii pneumonia in May 1992. He re
Resistant fungal infections in AIDS--an emerging problem.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:28 (abstract no. TC6). Unique Identifier : AIDSLINE ASHM5/94348950; Woolley I; Fuller A; Franklin C; Hale C; Vujovic O; Russell P; Spelman D; Spicer WJ; Department of Microbiology and Infectious Diseases, Alfred; Hospital, Prahran.; 1. CANDIDIASIS. We discuss 3 patients who developed severe symptomatic oral candidiasis. Clinical resistance to ketoconazole and fluconazole occurred over time, necessitating the use of intravenous amphotericin in two patients. Disc sensitivity testing of their isolates with Neosensitabs confirmed the resistance. 2. C
Transient ischaemic attacks (TIAs) in HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:29 (abstract no. TC7). Unique Identifier : AIDSLINE ASHM5/94348951; Brew BJ; Miller J; HIV Medicine Unit, St Vincent's Hospital, Australia.; OBJECTIVE: TIAs in HIV infection may occur as a result of an underlying infection or neoplasm and seemingly as a consequence of HIV itself. The frequency, clinical characteristics and results of investigations of those TIAs that occur independent of an underlying infection or neoplasm, however, have not been defined a
IgM antibodies against complex glycolipids of peripheral myelin in human immunodeficiency virus (HIV) infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:30 (abstract no. TC9). Unique Identifier : AIDSLINE ASHM5/94348952; Petratos S; Papadopoulos R; Turnbull V; Ayers M; Gonzales MF; Department of Anatomical Pathology, Royal Melbourne Hospital,; Parkville, Victoria.; IgM antibodies against complex glycolipids, in particular GM1, GD1b and GQ1b gangliosides have been detected in the plasma of patients with acute and chronic inflammatory demyelinating polyneuropathy, multi-focal motor neuropathy and motor neurone disease. Inflammatory demyelinating neuropathy is one of several neurop
Progressive multifocal leucoencephalopathy--the Fairfield Hospital experience.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:30 (abstract no. TC8). Unique Identifier : AIDSLINE ASHM5/94348953; Marshall C; Jennens I; Fairfield Hospital, Vic.; Progressive multifocal leucoencephalopathy (PML) is a cerebral infection caused by JC virus occurring in immunocompromised people. It has been well described in transplant recipients requiring immunosuppressive therapy and more recently in people immunosuppressed with human immunodeficiency virus (HIV). PML is estimat
Acetylation phenotype and hypersensitivity (HS) to trimethoprim-sulphamethoxazole (TMP-SMX) in HIV-infected patients.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:31 (abstract no. TC11). Unique Identifier : AIDSLINE ASHM5/94348954; Carr A; Gross A; Cooper DA; Centre for Immunology, St. Vincent's Hospital.; AIMS: HS to TMP-SMX is more common in patients with HIV infection. In those without HIV infection, HS is more common in those with a slow acetylator phenotype. A study was conducted to determine if the slow acetylation phenotype is associated with an increased risk of HS to TMP-SMX in patients with HIV. METHODS: Acety
Growth, characterisation and seroprevalence of an encephalitozoon SP from an AIDS patient with disseminated microsporidiosis.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:31 (abstract no. TC10). Unique Identifier : AIDSLINE ASHM5/94348955; Doultree JC; Ryan N; Druce J; Maerz A; Crowe S; Marshall J; Fairfield Hospital, Victoria.; Although microsporidia (MS) are important opportunistic pathogens in Acquired Immunodeficiency Syndrome (AIDS) patients, the in vitro characteristics and seroprevalence of many human species are poorly understood. Light microscopy and electron microscopy confirmed the presence of Encephalitozoon sp spores from a nasop
HIV related Mycobacterium tuberculosis (M.Tb) at Fairfield Hospital.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:32 (abstract no. FC1). Unique Identifier : AIDSLINE ASHM5/94348956; Wright EJ; Sarr A; Dwyer B; Hoy J; Fairfield Hospital, Vic.; OBJECTIVE: To review the clinical and laboratory features, management and outcome of M.tuberculosis in 18 HIV seropositive patients (2.5% of the total AIDS patients). METHODS: A retrospective review of case records and laboratory results of 16 male and 2 female patients attending Fairfield Hospital between January 198
Mediastinal tuberculosis with oesophageal perforation.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:32 (abstract no. FC2). Unique Identifier : AIDSLINE ASHM5/94348957; Dore G; St Vincent's Hospital, Darlinghurst, Sydney.; A 43 year old homosexual man, known seropositive for Human Immunodeficiency Virus (HIV) since 1984, with a CD4 Lymphocyte count of 40 x 109/ml, but with no prior Acquired Immunodeficiency Syndrome (AIDS) defining illness, presented with a three-week history of fevers, non-productive cough, pleuritic chest pain and dys
An unusual mycobacterial infection with osteomyelitis and skin lesions in an HIV patient.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:33 (abstract no. FC4). Unique Identifier : AIDSLINE ASHM5/94348958; Street AC; Fairfield Hospital, Vic.; Atypical mycobacterial infections other than those due to Mycobacterium avium complex are very uncommon in HIV patients. This report describes a case of Mycobacterium haemophilum (MH) infection with skin, bone and bloodstream involvement. A 42 year old HIV positive male with no prior AIDS-defining illness and a CD4 co
HIV-related mycobacterial infections in Queensland.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:33 (abstract no. FC3). Unique Identifier : AIDSLINE ASHM5/94348959; Konstantinos A; Dawson D; Patel AM; Mycobacterial Reference Laboratory, Queensland Health, Brisbane.; Human Immunodeficiency Virus (HIV) related mycobacterial infections in Queensland (Qld) were studied to define which mycobacteria contribute to HIV-related disease in Qld and implications for management. Mycobacteria were isolated from 112 HIV patients in Qld from 1/7/85-22/1/93 (Mycobacterium tuberculosis complex [MT
An Australian man who died of Pneumocystis carinii pneumonia complicating the acquired immunodeficiency syndrome in 1981.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:34 (abstract no. SC1). Unique Identifier : AIDSLINE ASHM5/94348960; Gerrard JG; McGahan SL; Milliken JS; Mathys JM; Royal Prince Alfred Hospital, Camperdown NSW.; OBJECTIVE: To present the earliest Australian case of the acquired immunodeficiency syndrome (AIDS) reported to date. CLINICAL FEATURES: A 72 year-old man developed a prolonged illness beginning in February 1981 characterised by anorexia, malaise, weight loss and an episode of dermatomal zoster. In July he became incr
Sinusitus in the person with HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:34 (abstract no. FC5). Unique Identifier : AIDSLINE ASHM5/94348961; van der Horst C; University of North Carolina.; Although sinusitus is not a fatal complication of HIV infection it is nonetheless a vexing problem for my patients. The incidence and severity worsens as CD4 count declines and is often associated with intranasal drug use. Higher IgE levels are associated with worse sinusitis and atopy. Treatment must include intranas
A case of HIV-2 infection in Victoria.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:35 (abstract no. SC2). Unique Identifier : AIDSLINE ASHM5/94348962; Breschkin A; Russell D; Crofts N; Locarnini S; Victorian Infectious Diseases Reference Laboratory, Fairfield; Hospital.; In June 1993 a serum sample from a high-risk patient was referred to the Victorian HIV Reference Laboratory at Fairfield Hospital for routine anti-HIV testing. The serum was repeatedly reactive (S/C0 = 3.7) by the Abbott HIV-1/HIV-2 EIA. Confirmatory testing by HIV-1 Western blot showed an indeterminate profile with m
Long term asymptomatic infection in six blood transfusion recipients with a single common donor.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:35 (abstract no. SC3). Unique Identifier : AIDSLINE ASHM5/94348963; Learmont J; Tindall B; Sullivan J; Dwyer D; Cunningham A; Wylie B; Red Cross Blood Transfusion Service NSW.; OBJECTIVES: To investigate the possibility of any additional recipients from the common donor and to further describe the health status of the group. RESULTS: 7 more donations were identified and 4 recipients traced and tested. 3 recipients of 1980 donations tested negative. A 77 year old female transfused in April 19
Zidovudine-resistant HIV-1 in primary infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:36 (abstract no. SC4). Unique Identifier : AIDSLINE ASHM5/94348964; Imrie A; Hurren L; Cunningham P; Vizzard J; Carr A; Gurusinghe A; Deacon N; Penny R; Cooper DA; Centre for Immunology, St Vincents's Hospital.; AIM: To assess the prevalence of transmitted zidovudine-resistant HIV in individuals who seroconverted after 1990. METHODS: HIV was isolated from 10 homosexual men with symptomatic primary HIV infection, and from their sexual partners when possible. Isolates obtained within the first two months following onset of symp
ZDV and ddI resistance in HIV isolated from patients enrolled in the alpha ddI trial.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:36 (abstract no. SC5). Unique Identifier : AIDSLINE ASHM5/94348965; Zheng N; McQueen PW; Hurren L; Imrie A; Evans L; Delaney SF; Penny R; Cooper DA; Centre for Immunology, St. Vincents Hospital, Sydney.; Progressive HIV disease continues to occur despite the therapeutic use of antiviral agents such as didanosine (ddI) a azidothymidine (ZDV) in the management of HIV infection. The means by which HIV-1 is able to overcome these chemotherapeutic challenges remains unclear at this time. It seems unlikely that a single cha
The Delta Study: one year later.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:37 (abstract no. SC6). Unique Identifier : AIDSLINE ASHM5/94348966; Rawlinson M; National Centre in HIV Epidemiology & Clinical Research,; Darlinghurst NSW.; STUDY OBJECTIVES AND METHODS: International randomised placebo controlled study comparing zidovudine ( AZT ) to combination AZT + dideoxycytidine( ddC ) or AZT + didanosine(ddI), in terms
Epidemiological aspects of HIV infection in Australian women.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:38 (abstract no. TE1). Unique Identifier : AIDSLINE ASHM5/94348967; Thackway SV; Furner V; Kaldor J; National Centre in HIV Epidemiology & Clinical Research,; Darlinghurst.; OBJECTIVE: To describe the characteristics of women with HIV-I infection in Australia . METHOD: The National Registry of Women with HIV Infection was established by merging the National HIV, AIDS, and Zidovudine Registries, using name codes and dates of birth. RESULTS: By March 1
The HIV risk-taking behaviour and perceptions of pregnant injecting drug users.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:38 (abstract no. TE2). Unique Identifier : AIDSLINE ASHM5/94348968; O'Neill K; Baker A; Cooke M; Collins EN; Wodak A; National Drug and Alcohol Research Centre, University of N.S.W.,; Kensington.; A sample of 94 pregnant injecting drug users (IDUS) were surveyed about their HIV risk-taking behaviour and perception of their risk of HIV infection. The women had been receiving methadone for varying periods, ranging from one week to 13 years (mean dose = 50 mg). In the month prior to interview, 93% had injected, 27
Perinatal exposure to HIV in Australia 1982-1993.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:39 (abstract no. TE4). Unique Identifier : AIDSLINE ASHM5/94348969; Cruickshank M; Ziegler JB; McDonald A; Prince of Wales Children's Hospital, Randwick.; OBJECTIVE: To describe the epidemiology of perinatal HIV exposure and transmission in Australia . METHODS: A registry of all cases of perinatal exposure to HIV was established using data retrieved retrospectively to test for possible factors affecting transmission rate, maternal c
Pregnancy and STDs: a study of the contraceptive practices of young sexually active women.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:39 (abstract no. TE3). Unique Identifier : AIDSLINE ASHM5/94348970; Lucke J; National Centre for HIV Social Research, Royal Brisbane Hospital,; Herston, Qld.; The aim of this study was to examine the relationship between condom use for disease prevention and the use of other contraceptives to prevent pregnancy among young women. Sexually active women aged between 16 and 24 who attended two Brisbane Family Planning Clinics were asked to complete a questionnaire. The questionc
Health care and costs of children with vertically acquired HIV infection in Australia.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:40 (abstract no. TE5). Unique Identifier : AIDSLINE ASHM5/94348971; Langdon P; Carlin JE; Ziegler J; Social Work Dept, Princess Margaret Hospital, Perth.; OBJECTIVES: To estimate lifetime costs of children with vertically acquired HIV infection and gather qualitative data on the clinical spectrum of disease and patterns of care of these children. METHODS: 33 of 38 eligible children were studied from 10 medical centres in 4 states and 1 territory. Study group included: cc
Heterosexual transmission of HIV: a European study.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:40 (abstract no. TE6). Unique Identifier : AIDSLINE ASHM5/94348972; Brunet JB; European Centre for the Epidemiological Monitoring of AIDS,; France.; From 1987 to 1991 563 couples were recruited from 9 countries in a cross-section study. 378 HIV(-) partners recruited in settings adapted for follow-up sectional study. 378 HIV(-) partners recruited in settings adapted for follow-up were included in the prospective study. At each interview partners were tested, intervc
Incidence of HBV and HCV in prison entrants in Victoria, 1991-92: evidence of a core risk group for HIV transmission.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:41 (abstract no. TE7). Unique Identifier : AIDSLINE ASHM5/94348973; Crofts N; Stewart T; Hearne P; Breschkin A; Locarnini S; Macfarlane Burnet Centre, Fairfield, Vic.; AIM: To measure incidence of blood-borne viruses among prison entrants, to assess risk in these populations for HIV transmission. METHODS: All entrants to Pentridge Prison between Oct91 and Oct92 were tested with consent for HIV/HBV/HCV; incidence rates were calculated for those tested more than once in the period. Lic
Unsafe sex among gay men: towards a new intervention strategy.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:41 (abstract no. TE8). Unique Identifier : AIDSLINE ASHM5/94348974; Gold R; Rosenthal D; Faculty of Education, Deakin University, Waurn Ponds, Vic.; OBJECTIVE. To examine the effectiveness of an intervention strategy based on men s justifications for breaking their own safe sex rules. METHOD: We report an intervention study in which 109 gay men who had slipped up (i.e., broken their safe sex rules by having unprotected intercourse) kept diaries of their sexual behc
HIV/AIDS education and sex education: do they encourage sexual experimentation in youth?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:42 (abstract no. TE9). Unique Identifier : AIDSLINE ASHM5/94348975; Grunseit AC; Kippax S; National Centre for HIV Social Research (MU), Macquarie; University, North Ryde, NSW, Australia.; INTRODUCTION: The provision of HIV/AIDS & sexuality education (SE) to young people is one of the most hotly debated issues facing educationists today. A major point of friction is whether exposure to HIV/AIDS &/or SE encourages sexual experimentation. To investigate this question, a literature review by the Mac
Predictors of uaic amongst a sample of homosexually active men.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:42 (abstract no. TE10). Unique Identifier : AIDSLINE ASHM5/94348976; Kippax S; Crawford J; Rodden P; National Centre for HIV Social Research, School of Behavioural; Science, Macquaire University, NSW.; OBJECTIVES: To investigate the predictors of unprotected anal intercourse with casual partner/s (uaic) amongst a sample of homosexually active men. METHOD: 2,583 homosexually active men (sex with a man within the last 5 years) were interviewed about their sexual practice, type of sexual partner/s, HIV test status, att
Changing pattern of HIV diagnosis in Victoria. The Victorian Collaborative Group on HIV and AIDS Surveillance.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:43 (abstract no. FE2). Unique Identifier : AIDSLINE ASHM5/94348977; Stevenson E; Crofts N; Gertig D; Breschkin A; Macfarlane Burnet Centre for Medical Research, Fairfield.; OBJECTIVES: To describe changes in the pattern of HIV diagnosis in Victoria. METHODS: Data, including exposure category, are collected on all Victorians newly diagnosed with HIV infection. For those cases not attributed to homosexual contact exposure is further clarified with the patient s doctor. RESULTS: For 1986 to
Patterns of AIDS and HIV infection in European countries.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:43 (abstract no. FE1). Unique Identifier : AIDSLINE ASHM5/94348978; Brunet JB; European Centre for the Epidemiological Monitoring of AIDS,; Saint-Maurice, France.; 96,000 AIDS cases were reported in Europe by 30 June 1993, and the population of HIV-infected persons is around 500,000. The European region has a common system for AIDS reporting, with an AIDS definition incorporating pulmonary tuberculosis, invasive cervical cancer, and recurrent pneumonia. Incidence of reported cas
HIV infection in recipients of blood products from donors with identified dates of HIV seroconversion.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:44 (abstract no. FE3). Unique Identifier : AIDSLINE ASHM5/94348979; Ashton L; Learmont J; Luo K; Wylie B; Stewart G; Kaldor J; National Centre for HIV Epidemiology and Clinical Research,; Sydney.; OBJECTIVES: To study factors that influence survival time in HIV-infected transfusion recipients. METHODS: A registry of people with transfusion acquired HIV infection (TAHIV) in NSW was established by the Red Cross Blood Transfusion Service; annual follow-up was implemented in 1990. From the registry a group of 6 don
Occupational exposure to blood and body fluids in Australian hospitals.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:44 (abstract no. FE4). Unique Identifier : AIDSLINE ASHM5/94348980; MacDonald M; Elford J; Kaldor J; National Centre in HIV Epidemiology and Clinical Research,; Darlinghurst, NSW.; OBJECTIVE: To describe the reporting of occupational exposure to blood and body fluids in Australian hospitals. METHODS: In December 1992, questionnaires were sent to 70 teaching hospitals throughout Australia. Information was sought on procedures for the reporting and management of occupational exposure to blood and
The acquired immunodeficiency syndrome in the Northern Territory of Australia: an impending Fourth World epidemic?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:45 (abstract no. FE5). Unique Identifier : AIDSLINE ASHM5/94348981; Heath T; Bowden F; Sheppard K; Currie B; Royal Darwin Hospital, Casuarina, N.T.; Until recently HIV infection in the Northern Territory (NT) has been an urban disease of non Aboriginal people. Risk factors for HIV infection and indicator diseases for AIDS have been similar to those found in other Australian States. There is concern however, given the demographics of other sexually transmitted dise
Assessing health service interventions in HIV-disease.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:46 (abstract no. SE1). Unique Identifier : AIDSLINE ASHM5/94348982; Beck EJ; Academic Department of Public Health, St. Mary's Hospital Medical; School, Imperial College, UK.; AIM: To assess the effectiveness of health service interventions in HIV-disease, pathophysiological indices need to be interpreted within the context of service utilisation and specific treatment received. This interrelationship will be demonstrated in terms of outcome of first AIDS defining (index) episode of Pneumoc
The NSW HIV/AIDS Ambulatory Services casemix study.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:46 (abstract no. SE2). Unique Identifier : AIDSLINE ASHM5/94348983; Donnelly C; Stewart K; Starkey G; Information Centre, NSW Health Department, North Sydney.; This study examines the range of ambulatory care services provided to people with HIV, or screened for HIV. It involved a 2 or 3 week prospective data collection of services provided on an ambulatory basis at 14 hospitals and clinics, including one private general practice. Data for ambulatory consultations, HIV scree
Changing age at diagnosis of HIV/AIDS among gay men in Victoria, 1986-92.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:47 (abstract no. SE4). Unique Identifier : AIDSLINE ASHM5/94348984; Crofts N; Stevenson E; Thompson S; Macfarlane Burnet Centre, Fairfield, Vic.; AIM: To assess trends in age at diagnosis of HIV/AIDS among Victorian gay men METHODS: Geometric mean ages at diagnosis of HIV infection/AIDS were calculated RESULTS: Mean age at diagnosis of AIDS has been consistently about 4.5 years greater than at diagnosis of HIV infection. Both have been increasing steadily over
Length of phases of HIV disease defined by CD4 lymphocyte count.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:47 (abstract no. SE3). Unique Identifier : AIDSLINE ASHM5/94348985; Carlin JB; Clinical Epidemiology & Biostatistics Unit, Royal Children's; Hospital, Melbourne.; OBJECTIVES: To review and synthesize available knowledge on the usual lengths of time spent by persons with HIV in each of the clinically defined phases: (1) CD4 count > 500 (x 10(6)/l), (2) CD4 count 200-500, (3) CD4 count
Controlled evaluation of a brief intervention for HIV prevention among injecting drug users not in treatment.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:48 (abstract no. SE5). Unique Identifier : AIDSLINE ASHM5/94348986; Baker A; Kochan N; Dixon J; Heather N; Wodak A; National Drug and Alcohol Research Centre, Kensington, NSW.; This study evaluated the effectiveness of a brief intervention (BI) in reducing HIV risk-taking behaviours among 200 injecting drug users (IDU) not enrolled in any form of treatment for drug dependence. Subjects were randomly assigned to either a one-session cognitive-behavioural intervention or to a no-treatment cont
Depressive symptoms in patients with HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:49 (abstract no. TP2). Unique Identifier : AIDSLINE ASHM5/94348987; Judd FK; Mijch AM; Fairfield Hospital, Melbourne.; OBJECTIVES: To determine the frequency of depressive symptoms and suicidal ideation amongst patients with HIV/AIDS attending an outpatient clinic in suburban Melbourne. METHODS: All patients (unless non-English speaking) attending one physician at Fairfield Hospital over a 4 month period were asked to complete the 21
Psychiatric morbidity in HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:49 (abstract no. TP1). Unique Identifier : AIDSLINE ASHM5/94348988; Strain J; The psychiatric morbidities that accompany HIV disorders stem from three sources: 1) the virus impact on the central nervous system; 2) chemotherapeutic agents prescribed for: the virus, other infections, and/or the neoplastic disorders; and, 3) acute psychological reactions to fears of or knowledge of having the dis
Depressive pseudodementia. V. Organic cerebral disease.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:50 (abstract no. TP3). Unique Identifier : AIDSLINE ASHM5/94348989; Cockram AM; Judd FK; Mijch AM; Fairfield Hospital, Melbourne.; A 30 year homosexual man, was referred for assessment of depressed mood. He had recently been an inpatient for treatment of Mycobacterium avium bacteremia, but had responded well to treatment and had returned to full time employment. He described low mood of several months duration, anxiety, sleep disturbance, poor ap
Patterns of AIDS bereavement.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:50 (abstract no. TP4). Unique Identifier : AIDSLINE ASHM5/94348990; Kelly B; Raphael B; Hickman B; Statham D; Ross M; National Centre for HIV Social Research, University of QLD.; AIMS: A prospective study of bereavement following an AIDS death is being conducted, investigating patterns of bereavement response, risk factors to pathological grief and comparison with a group of cancer bereaved individuals. METHOD: 32 AIDS and 41 cancer bereaved subjects (mostly parents and partners/spouse) comple
Coping strategies and psychosocial context of HIV positive young gay men.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:51 (abstract no. FP2). Unique Identifier : AIDSLINE ASHM5/94348991; Goggin M; Centre for the Study of STDs, LaTrobe University, Carlton South.; OBJECTIVE: To examine the strategies young men use in coping with being HIV positive and relate these to the young men s psychosocial context. METHOD: 101 HIV positive young gay men aged 17 to 30 (mean age = 26.0 years) were recruited from non-clinical settings and interviewed about their coping strategies, which are
Personal relationships and HIV/AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:51 (abstract no. FP1). Unique Identifier : AIDSLINE ASHM5/94348992; O'Loughlin B; Cannold L; Hickman B; McLean S; Woolcock G; AIM: To investigate ramifications of HIV/AIDS upon relationship networks and the role of these relationships in developing meaning and coping mechanisms for living with HIV/AIDS. METHODOLOGY: Semi-structured interviews with fifteen gay men. Thematic content analysis used on transcribed tapes. RESULTS: A significant nu
Constructing a sexual self: sexual self conceptions and their influence on HIV/AIDS risk behaviours.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:52 (abstract no. FP3). Unique Identifier : AIDSLINE ASHM5/94348993; Buzwell S; Centre for the Study of STDs, La Trobe University, Carlton South.; OBJECTIVE: To examine the context in which adolescents make their sexual decisions and how these perceptions impact upon their behaviour, and in particular those behaviours that put adolescents at risk of contracting HIV. METHOD: Questionnaires were administered to 870 adolescents (aged to 15 to 18) attending two Melb
Knowing about AIDS: from information to understanding.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:52 (abstract no. FP4). Unique Identifier : AIDSLINE ASHM5/94348994; Rosenthal D; Centre for the Study of STD, La Trobe University, Carlton South.; OBJECTIVE: To document developmental changes in adolescents knowledge about AIDS. METHOD: Pre-adolescents and adolescents (aged 10 to 18 years) were tested on their knowledge of HIV/AIDS. Knowledge was assessed using a self-report questionnaire (examining levels of factual knowledge) and a semistructured interview (e
HIV, confidentiality and the duty to warn.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:53 (abstract no. SP2). Unique Identifier : AIDSLINE ASHM5/94348995; Ryan C; Department of Psychiatry, Nepean Hospital, Penrith, NSW.; Taking the hypothetical example of an HIV positive dentist who wishes to continue practicing, this paper explores the ethical issues facing a clinician who must decide whether or not to breach confidentiality when others are placed at risk of contracting HIV. In addition the paper explores the common law and statutory
Neuropsychological impairment in AIDS dementia complex (ADC): preliminary results.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:53 (abstract no. SP1). Unique Identifier : AIDSLINE ASHM5/94348996; Dunbar N; Brew B; National Centre in HIV Epidemiology and Clinical Research,; University of New South Wales, Sydney.; AIM: To develop a predictive model of neuropsychological impairment in ADC. METHOD: HIV+ homosexual men were recruited. Eligibility criteria were: CD4+ cell count
Alcoholism as a risk factor.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:54 (abstract no. SP5). Unique Identifier : AIDSLINE ASHM5/94348997; Strain J; Individuals who use alcohol have been found to be at higher risk for HIV infection than those who do not. The San Francisco Gay Men s Health Study, the Multicenter AIDS Cohort Study (MACS), and the Newark Medical Center Alcoholic Assessment Study, all describe that the use of alcohol, and, in particular, if there is a
Counselling for difficult patient.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:54 (abstract no. SP4). Unique Identifier : AIDSLINE ASHM5/94348998; Waters B; Department of Psychiatric Services, St. Vincent's Hospital,; Sydney.; HIV has not afflicted a representative cross section of the community. IDU s have a greatly increased risk of having pre-existing personality disorder and/or unstable interpersonal relationships. HIV+ male homosexuals are more likely than their HIV-counterparts to have a history of prior mental health interventions, p
Bacillary angiomatosis.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:55 (abstract no. FCP1). Unique Identifier : AIDSLINE ASHM5/94348999; Kamarulzaman A; Armes J; Fairfield Infectious Disease Hospital, Victoria.; CASE REPORT: A 28 year old homosexual man with AIDS presented with multiple skin lesions on the dorsum of the foot. A biopsy of one of the lesions revealed histological changes consistent with bacillary angiomatosis . Treatment with erythromycin was instituted which resulted i
Undiagnosed HIV infection presenting with disseminated primary cutaneous nocardiosis in the Northern Territory.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:55 (abstract no. FCP2). Unique Identifier : AIDSLINE ASHM5/94349000; Patel A; Bowden F; Heath T; Fisher D; Currie B; HIV/STD Unit, Communicable Diseases Centre, Casuarina NT.; Nocardia asteroides is a soil saprophyte. Human infection is predominantly acquired by inhalation and is not uncommonly associated with immunosuppression due to therapeutic drugs, haematological malignancy or general debility as occurs with chronic alcoholism. Surprisingly few cases of nocardiosis in HIV infected pati
Disseminated Penicillium marneffei: presenting illness of advanced HIV infection in a Burmese immigrant. Successful treatment with early recognition.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:56 (abstract no. FCP4). Unique Identifier : AIDSLINE ASHM5/94349001; Heath T; Patel A; Fisher D; Currie B; Royal Darwin Hospital, Casuarina NT.; Penicillium marneffei is a dimorphic fungus occasionally causing infection in immunocompetent individuals, but more frequently infecting patients with impaired T-cell immunity, particularly in association with HIV. Infection appears to be confined to residents of, or travellers to, south-east Asia, especially northern
Pyomyositis and HIV: an infrequent yet recognised association.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:56 (abstract no. FCP3). Unique Identifier : AIDSLINE ASHM5/94349002; Hellard ME; Street AC; Royal Melbourne Hospital.; Skeletal muscle abscesses (pyomyositis) are an uncommon but well recognised problem in human immunodeficiency virus (HIV) patients. This report discusses a case of an ileopsoas abscess in an HIV patient and reviews the experience with HIV associated pyomyositis in patients attending the Royal Melbourne & Fairfield
From grim reaper to mainstream.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:57 (abstract no. SSW2). Unique Identifier : AIDSLINE ASHM5/94349003; Hoy KW; Morehead S; From Grim Reaper to mainstreaming of HIV/AIDS services, a contextual analysis of service delivery based on policy documents and strategies in both the local arena and in Australia . The less obvious social work contributions to policy planning and practice applications will be id
The contribution of sociobehavioural research to AIDS prevention and treatment.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:57 (abstract no. SSW1). Unique Identifier : AIDSLINE ASHM5/94349004; Jackson AC; James C; HIV/AIDS Sociobehavioural Research Unit, School of Social Work,; Melbourne University.; This paper illustrates the contribution of sociobehavioural research to the development of HIV/AIDS prevention education programs, and to the development of relevant service models for treatment. It focuses on work undertaken in the HIV/AIDS Sociobehavioural Research Unit at Melbourne University, and presents a range
Issues: infectious diseases service.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:58 (abstract no. SSW3). Unique Identifier : AIDSLINE ASHM5/94349005; O'Connor SP; Infectious Diseases Service, Royal Melbourne Hospital.; In this paper there will be a short case presentation highlighting some of the many issues that may arise when working with a member of this client group. Some ethical issues are outlined and responded to and important points to cover with all users will be outlined. Finally, a suggested framework for facilitating eff
Medically acquired HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:58 (abstract no. SSW4). Unique Identifier : AIDSLINE ASHM5/94349006; Mudge L; Haemophilia Treatment Centre, Alfred Hospital, Prahran.; From 1980-85 approximately 240 people with coagulation disorders were infected with HIV through treatment with blood products. This paper outlines the progression and psychosocial impact on the 60 patients currently treated at the Alfred Hospital for medically acquired HIV. There are particular psychosocial issues rel
AIDS dementia: educational needs of carers.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:59 (abstract no. TPa1). Unique Identifier : AIDSLINE ASHM5/94349007; Grigg M; Fairfield Hospital, Victoria.; Much of the focus on AIDS dementia has concentrated on epidemiological, diagnostic and treatment issues. Another important dimension is the educational needs of carers. Carers often experience a range of difficulties when caring for the person with AIDS dementia. These difficulties
HIV dementia--challenge to services.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:59 (abstract no. TPa2). Unique Identifier : AIDSLINE ASHM5/94349008; Briton C; Fensling L; Watson J; Social Work Department, Fairfield Hospital, Vic.; Every diagnosis of AIDS has repercussions that are widespread as well as deeply felt. AIDS Dementia Complex brings a particularly wide range of issues which are magnified and accentuated when the person is otherwise physically well. The authors look in same detail at personal, social, ethical, legal and practical issu
Pain in patients with advanced AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:60 (abstract no. SPa2). Unique Identifier : AIDSLINE ASHM5/94349009; Glare P; Palliative Care Service, Royal Prince Alfred Hospital,; Camperdown, NSW.; There has been little systematic evaluation of pain or its treatment in patients with HIV/AIDS. The charts of all HIV admissions to the palliative care ward at Eversleigh Hospital, Petersham were reviewed for pain and analgesic use. There were 104 admissions of 68 patients. 65 (96%) were males. 63 (93%) had stage IV d
What is the best model for AIDS palliative care?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:60 (abstract no. SPa1). Unique Identifier : AIDSLINE ASHM5/94349010; Malcolm JA; Nineways Specialist Clinic, Broadmeadow, NSW.; In one of competing models AIDS palliation begins when active treatment ceases and responsibility for care passes from a treatment to a palliative care service. This model (based on cancer care) limits service overlap and is economical, but creates practical and ethical difficulty in defining when to cease active trea
In vitro activity of protease inhibitors against HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:62 (abstract no. TB2). Unique Identifier : AIDSLINE ASHM5/94349011; Birch C; Tachedjian G; Tyssen D; Grobelny D; Virology Department, NCHVR, Fairfield, Victoria.; HIV encodes a novel aspartyl protease that represents an important virus-specific target for inhibitors. We have been studying a series of transition state analogue inhibitors designed and synthesised in Australia that have potent and specific activity against HIV replication in
Isolation and zidovudine sensitivity of HIV-1 isolated from post-mortem tissue.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:62 (abstract no. TB1). Unique Identifier : AIDSLINE ASHM5/94349012; McGavin CH; Land SA; Birch CJ; Virology Department, Fairfield Hospital, Victoria.; We attempted to isolate HIV from post-mortem tissues (CSF, spleen, spinal cord, brain and lymph node) of 64 patients. The overall isolation rate was low (48%) compared to a rate of 95% from peripheral blood mononuclear leucocytes (PBMC s) from the same patients when they were alive. This difference appeared to be due
In vitro generation and characterisation of foscarnet-resistant HIV-1.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:63 (abstract no. TB3). Unique Identifier : AIDSLINE ASHM5/94349013; Tachedjian G; Mills J; Birch C; Macfarlane Burnet Centre, Fairfield Hospital, Australia.; Foscarnet (phosphonoformic acid) is a pyrophosphate analogue used for the treatment of CMV retinitis and acyclovir-resistant herpes simplex infections in AIDS patients. Foscarnet is also an inhibitor of HIV, and therefore long-term administration in patients may
Novel mutations associated with HIV-1 resistance to AZT.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:63 (abstract no. TB4). Unique Identifier : AIDSLINE ASHM5/94349014; Hooker DJ; Gurusinghe AD; Land SA; Birch CJ; McGavin C; Tachedjian G; Deacon NJ; AIDS Molecular Biology Lab, NCHVR Macfarlane Burnet Cent. for; Med. Res., Fairfield, Vic.; Associated with AZT therapy is the emergence in patients of drug resistant isolates of HIV-1. Certain mutations observed in the reverse transcriptase (RT) gene/protein upon AZT therapy, namely at codons 41, 67, 70, 215 and 219, have previously been re
Potential molecular competitors for HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:64 (abstract no. TB5). Unique Identifier : AIDSLINE ASHM5/94349015; Pieczenik G; Cook College, Rutgers University, New Brunswick, NJ 08903.; The phenotype of clinically non-pathogenic strains of HIV-1 do not differ dramatically from the clinical picture of pathogenic strain of HIV-1. A differential diagnosis of a clinical infection with a non-pathogenic strain is the observation of infectious hypo virulence. Infectious hypo virulence is an infection of a p
Uptake and expression of HIV-1 B isolated thymic dendritic cells.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:64 (abstract no. TB6). Unique Identifier : AIDSLINE ASHM5/94349016; Lowe M; Sotzic F; Shortman K; Cameron P; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; Dendritic cells (DC) are the major antigen presenting cells for the induction of T cell mediated immune responses. We have found DC isolated from peripheral blood by sorting are CD4- and do not allow viral entry by T cell or macrophage tropic isolates of HIV-1. DC are functionally and phenotypically heterogeneous and
Factors involved in susceptibility of macrophages but not fresh monocytes to HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:65 (abstract no. TB8). Unique Identifier : AIDSLINE ASHM5/94349017; Maerz A; Sonza S; Crowe S; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; OBJECTIVES: To determine the time post isolation at which monocyte/macrophages show increased susceptibility to infection with HIV, and identify the stage of viral replication which is blocked in cells which are less susceptible. METHODS: Monocytes were isolated from HIV seronegative buffy coats by density gradient ce
Molecular mechanisms of interleukin 4 stimulation of HIV mRNA expression in human monocytes.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:65 (abstract no. TB7). Unique Identifier : AIDSLINE ASHM5/94349018; Naif H; Mathijs JM; Shon MH; Chang J; Cunningham T; Virology Department, Westmead Hospital, National Centre in HIV; Research, Sydney, Australia.; HIV-1 can productively infect mononuclear phagocytes derived from blood bone marrow, brain and lung. Interleukin 4 (IL-4) and tumor necrosis factor alpha (TNF alpha) have previously been shown to stimulate HIV replication in monocytes and macrophages (1,2). The mechanism of IL-4 stimulation was investigated and compar
HIV-1 integration in peripheral blood monocytes and cultured macrophages.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no. TB9). Unique Identifier : AIDSLINE ASHM5/94349019; Sonza S; Maerz A; Mills J; Crowe S; AIDS Pathogenesis Research Unit, NCHVR, Macfarlane Burnet Centre; for Medical Research, Fairfield, Victoria.; Although integration has been clearly demonstrated in HIV-1 infected lymphocytes, there has been only a single unconvincing report of this in primary macrophages. We have used various polymerase chain reaction-based techniques to determine whether and when this step in the infection process takes place in monocytes an
Twin studies suggest a host cell genetic effect on HIV replication in human monocytes/macrophages.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no. TB10). Unique Identifier : AIDSLINE ASHM5/94349020; Chang J; Li S; Sullivan J; Kazazi F; Naif H; Cunningham T; Virology Department, Westmead Hospital, National Centre in HIV; Research, Sydney, Australia.; Biological and genetic variability is a prominent feature of HIV strains, especially in tropism, syncytial formation and replicative capacity. Preliminary studies also suggested there were variable host cell effects on HIV replication in monocytes. Therefore we used monocytes from identical (ID) twins and age matched
A cis-acting repressive sequence that overlaps the Rev-responsive element of HIV-1 regulates nuclear retention of env mRNAs independently of splice signals.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:67 (abstract no. FB1). Unique Identifier : AIDSLINE ASHM5/94349021; Brighty DW; Rosenberg M; SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania; 19406-0939.; The Rev protein of HIV-1 binds to an RNA structure, the Rev-Responsive Element (RRE), and enhances expression of the viral structural genes by relieving the nuclear sequestration of incompletely spliced viral transcripts. It has been due to the activity of cis-acting repressive sequence (CRS) elements and to inefficie
Impaired phagocytosis and killing of Toxoplasma gondii by HIV-infected macrophages.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:67 (abstract no. TB11). Unique Identifier : AIDSLINE ASHM5/94349022; Hewish M; Biggs BA; Kent S; Crowe SM; Walter and Eliza Hall Institute of Medical Research.; The protozoan parasite, Toxoplasma gondii, causes asymptomatic but persistent infection in 30-45% of the Australian population, and reactivation of latent infection is a major cause of morbidity and mortality in AIDS patients. Impaired macrophage function during HIV-infection may play a role in allowing reactivation.
Involvement of the c-myb proto-oncogene in HIV-1 gene expression.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:68 (abstract no. FB3). Unique Identifier : AIDSLINE ASHM5/94349023; Churchill MJ; Ramsay RG; Deacon NJ; AIDS Molecular Biology Unit, NCHVR, Macfarlane Burnet Centre,; Parkville, Australia.; Recently it has been shown that the HIV-1 LTR contains a high affinity Myb-binding site, (Dasgupta et al, PNAS 1990; 87:8090). The proto-oncogene c-myb, is expressed in many cell types including hemopoietic cell lines and tissues where it is thought to be associated with the regulation of proliferation and differentia
Mutually dependent, concurrent RNA and DNA synthesis during early phase HIV replication.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:68 (abstract no. FB2). Unique Identifier : AIDSLINE ASHM5/94349024; Kok TW; Li P; Burrell CJ; Div. of Medical Virology, NCHVR, Institute of Medical and; Veterinary Science, Adelaide, South Australia.; In a cell-to-cell HIV infection model it was found that there were two distinct phases of induced HIV RNA synthesis. The first phase (4h - 12h pi) was marked by a significant increase in only the genomic-length viral RNA. The second, phase (24h onwards) comprised a dramatic increase in the levels of all three species
Activation of the NF-kappa-B p105 promoter by HTLV-I Tax.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:69 (abstract no. FB5). Unique Identifier : AIDSLINE ASHM5/94349025; Lambert P; Ludford M; Deacon N; Doherty R; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; The transactivator protein of HTLV-I, Tax, activates transcription not only from the HTLV-I LTR, but also from several other viral and cellular promoters. One of its targets is the LTR of HIV; HIV replication is enhanced in HTLV-I-infected cell lines, and there is increasing evidence that patients infected with both v
Multiple proteins interact over the USF binding site in the human immunodeficiency virus type 1 long terminal repeat.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:69 (abstract no. FB4). Unique Identifier : AIDSLINE ASHM5/94349026; Tanskanen E; Deacon N; Churchill M; Doherty R; Macfarlane Burnet Centre for Medical Research, Fairfield, Vic.; HIV-1 gene expression and viral replication are regulated by several viral and cellular proteins, most commonly through binding sites within the long terminal repeats (LTRs) of the viral genome. We have examined binding of transcription factors over the region from nt -141 to nt -180 relative to transcription initiati
Double-stranded strong-stop DNA and HIV reverse transcription.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:70 (abstract no. FB7). Unique Identifier : AIDSLINE ASHM5/94349027; Li P; Stephenson A; Kuiper L; Burrell CJ; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.; Using a synchronized, one-step HIV infection model, we report here the detection in acutely infected cells of a novel double-stranded strong-stop HIV DNA with a discrete length of approximately 650 base pairs, commencing at or near the left hand end of the right hand U3 region of the HIV long terminal repeat. The plus
Reverse transcription factories in cell-to-cell HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:70 (abstract no. FB6). Unique Identifier : AIDSLINE ASHM5/94349028; Karageorgos LE; Li P; Burrell CJ; National Centre for HIV Virology Research, Institute of Medical; and Veterinary Science, Adelaide, South Australia.; Reverse transcription, the conversion of the single-stranded RNA genome into double-stranded DNA molecule, is a major event in the life cycle of retroviruses. We have shown that full-length unintegrated viral DNA in the cytoplasm of HIV infected cells is found in association with the viral proteins reverse transcripta
Analysis of HIV-induced apoptosis: viral infection is required.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:71 (abstract no. SB1). Unique Identifier : AIDSLINE ASHM5/94349029; Corbeil J; Howell ML; Richman DD; University of California San Diego, Department of Pathology, La; Jolla 92093-0679.; Lymphoblastoid T cell lines (SupTl, MT-2 and WE 17/10) and preparations of peripheral blood lymphocytes (CD4+ T cells enriched to 90%) were exposed to HIV-1LAI either untreated or inactivated with 4 -aminomethyltrioxsalen (10 approximately g/mL) in combination with long-wave-length ultraviolet light (320-400nm) to ren
HIV-1 auxiliary protein Nef down-regulates expression of cellular receptors and factors involved in signalling.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:71 (abstract no. SB2). Unique Identifier : AIDSLINE ASHM5/94349030; Greenway A; Allen K; McPhee D; AIDS Cellular Biology Unit, Macfarlane Burnet Centre, NCHVR,; Melbourne, Victoria, Australia.; The function of HIV-1 Nef protein, although poorly understood, has been implicated in the progression of HIV infection to AIDS. To address the effect of Nef on cellular activity, Nef protein was introduced into cells by a sophisticated electroporation technique. Electroporation of HIV-1 Nef reduced the expression of c
HIV-1 grown in CD4-bearing cells may be coated with CD4.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:72 (abstract no. SB4). Unique Identifier : AIDSLINE ASHM5/94349031; Hartley C; Gilbert M; Elbeik T; Crowe S; Mills J; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; Using a CD4-capture enzyme-immunoassay, HIV-1 (SF33) grown in CD4-expressing T-lymphoblastoid cells (SF33-VB) were found to contain 100-10,000 fold less reactive gp 120 than HIV-1 (SF33) grown in CD4-negative human neuroblastoma cell (SF33-NB), despite similar virus titres. Virus strains grown in CD4-expressing cells
Surface protein analysis of a non-syncytium inducing HIV-1 isolate.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:72 (abstract no. SB3). Unique Identifier : AIDSLINE ASHM5/94349032; Fryer KM; Deacon NJ; Land SA; McPhee DA; Macfarlane Burnet Centre for Medical Research, Fairfield, Vic.; An Australian HIV-1 isolate that is non-syncytium inducing (NSI), and has reduced cytopathic effect on peripheral blood mononuclear cells (PBMCs) has been characterised. The biochemical properties and DNA sequence of this isolate (243925) were compared with a typical syncytium inducing (SI) isolate 228200. Both isolat
Effects of GMCSF and HIV-1 infection on the expression of CD11a on monocyte-derived macrophages.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:73 (abstract no. SB5). Unique Identifier : AIDSLINE ASHM5/94349033; Stent G; Crowe SM; Macfarlane Burnet Centre for Medical Research, Fairfield,; Australia.; OBJECTIVES: To examine the kinetics and regulation of CD11a on monocytes (MO) and macrophages (Mphi). METHODS: Mphi were isolated from buffy coats (n = 4) by density gradient centrifugation and plastic adherence or elutriation. (1) MO were incubated with varying concentrations of GMCSF (0-500U/ml) on the day of isolat
Production of HIV DNA, mRNA and protein without infectious virus in growth-arrested cells.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:73 (abstract no. SB6). Unique Identifier : AIDSLINE ASHM5/94349034; Kiernan R; Purcell D; McPhee D; National Centre in HIV Virology Research, Cellular Biology Unit,; Macfarlane Burnet Centre for Medical Research, Fairfield, Vic.; We have previously shown that an HTLV-I transformed cell line, MT-2, arrested in cell division and proliferation by treatment with 5 mM hydroxyurea could not be productively infected by HIV. Both release of cell-free virus and cell-cell fusion were found to be inhibited. Step-wise analyses of HIV replication have show
Fried green pentamidine at the Carlton Clinic Cafe. Nurse practitioners, HIV infection and private general practice: the way of the future?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:74 (poster no. 1). Unique Identifier : AIDSLINE ASHM5/94349035; Mitchell J; Bradford D; Russell D; Murray P; Carlton Clinic, Vic, Australia.; OBJECTIVES: To investigate the possible role of a nurse counsellor (NC) in improving cost effective service delivery for HIV-positive patients in the private general practice setting. METHODS: A NC was employed under a GP demonstration practice grant by the Commonwealth Department of Health for 6 months in an establis
Who calls the Sydney HIV/AIDS Information Line?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:74 (poster no. 2). Unique Identifier : AIDSLINE ASHM5/94349036; Harvey-Sutton P; Nelson M; Tomkins M; Albion St. Centre, Surry Hills.; Since its inception in 1985 the NSW HIV/AIDS Information Line receives an average of 100 calls per day from which statistics are gathered about the caller s sex, location, sexuality, transmission issues, risk behaviour, nature of enquiry, and referral/action taken. These statistics can be used to assess areas of HIV e
Occupational therapy and working with people with HIV/AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:75 (poster no. 4). Unique Identifier : AIDSLINE ASHM5/94349037; Jackson M; Black K; Taylor K; Nicholas S; Community Health Services, Central and Eastern Sydney Area; Health.; Discussion of various topics including: 1. The changing role of occupational therapy (O.T.) in HIV/AIDS to less traditional areas. 2. O.T. treatment and intervention in AIDS related illness. 3. Issues of energy conservation and fatigue management. 4. The role of stress management and meditation. 5. Issues of home rese
Physiotherapy: enhancing primary care & improving quality of life.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:75 (poster no. 3). Unique Identifier : AIDSLINE ASHM5/94349038; Zacka M; Physiotherapy Dept. Royal Prince Alfred Hospital, Camperdown,; NSW.; The physiotherapist is integral to the multidisciplinary team working in HIV. The role of the physiotherapist is often poorly understood and therefore frequently under utilised. The physiotherapist offers intervention in the following areas: 1. Respiratory disorders. Obtaining sputum specimens, treatment of pneumonias
HIV counselling training for general practitioners.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:77 (poster no. 8). Unique Identifier : AIDSLINE ASHM5/94349039; Lipp J; Stynes R; Jackson S; Catter L; Moloney B; Phillips G; Anita Morawetz HIV Counselling and Training Centre (A.M. Centre),; Parkville.; OBJECTIVES: To train general practitioners in counselling skills as an essential component of their ongoing management of HIV patients in a community setting. METHODS: A state-funded training program in HIV counselling has been run by the A.M. Centre for the past 3 years. This is a one year, half day/week course open
Undergraduate education in HIV medicine.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:77 (poster no. 7). Unique Identifier : AIDSLINE ASHM5/94349040; Cross G; Savage J; Department of Microbiology, Monash Medical School, Alfred; Hospital, Prahran, Vic.; In 1992, an elective programme was introduced into the undergraduate medical curriculum at Monash University. This programme An Introduction to HIV Medicine aimed to provide a broader view than that provided by ward-based clinical teaching. It included perspectives from general practice, counsellors, people living w
Design and implementation of a general practitioner program for the prevention and treatment of HIV infection.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:78 (poster no. 9). Unique Identifier : AIDSLINE ASHM5/94349041; McCoy R; Mijch A; Grierson J; Perry G; Fairfield Hospital, Melbourne Australia.; OBJECTIVES: To increase the number of general practitioners able to successfully manage HIV patients in a community setting. METHODS: A state-funded education program was designed by a multidisciplinary team consisting of medical specialists, general practitioners, people with HIV infection and a range of HIV/AIDS edu
The management and prevention of HIV in the rural setting: Eaglehawk Health Centre.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:78 (poster no. 10). Unique Identifier : AIDSLINE ASHM5/94349042; Hajicosta T; Duke R; Eaglehawk and Long Gully Community Health Centre, Bendigo,; Victoria, Australia.; OBJECTIVES: To improve the prevention of HIV and establish treatment services for people with HIV infection in the Bendigo Region. METHODS: Six general practitioners, two nurses, one social worker, from Eaglehawk Health Centre 150km north of Melbourne Victoria participated in incorporating the management of HIV infect
Multicultural HIV/AIDS: a model of care.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:79 (poster no. 12). Unique Identifier : AIDSLINE ASHM5/94349043; Todaro P; Eisenberg M; Multicultural HIV/AIDS Education and Support Project, Camperdown,; NSW.; The Multicultural HIV/AIDS Education and Support Project was established in May 1991. The Project is funded by the AIDS Bureau, NSW Department of Health, and is auspiced by the Central Sydney Area Health Service. It targets 15 non-English speaking communities (identified by the AIDS Bureau). The Multicultural HIV/AIDS
The HIV/AIDS anti-discrimination campaign: was it news?: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:79 (poster no. 11). Unique Identifier : AIDSLINE ASHM5/94349044; Bell GK; Albion Street Centre, Surry Hills.; OBJECTIVES: To examine the response of the major metropolitan newspapers to the Federal Government s HIV/AIDS Anti-discrimination campaign in January, 1993; to explore current attitudes in the media towards AIDS. METHODS: Interviews with journalists involved in writing about the campaign and with members of the Austra
Hypermedia and HIV/AIDS education for medical students and general practitioners.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:80 (poster no. 14). Unique Identifier : AIDSLINE ASHM5/94349045; Kidd M; Bearman M; Cesnik B; Turnidge J; McCoy R; Unit of Medical Informatics, Monash University, East Bentleigh,; Australia.; This oral presentation will outline the development and evaluation of a major hypermedia education package on the management and prevention of HIV/AIDS. The HIV Hypermedia Medical Education Project has been created to provide a comprehensive computer based teaching program for Australian medical students and general p
The HIV Hypermedia Medical Education Project.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:80 (poster no. 13). Unique Identifier : AIDSLINE ASHM5/94349046; Bearman M; Kidd M; Cesnik B; Turnidae J; McCoy R; Unit of Medical Informatics, Monash University, East Bentleigh,; Australia.; This is an interactive computer based poster demonstration. It provides conference participants with the opportunity to engage in a hands on exploration of this richly linked hypertext program and its exciting multimedia components. The HIV Hypermedia Medical Education Project has been created to provide a comprehensi
Attitudes, beliefs and knowledge about HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:81 (poster no. 15). Unique Identifier : AIDSLINE ASHM5/94349047; Carman J; Roth N; Ross M; Painter L; HIV/AIDS Services, Camperdown, NSW.; The Central Sydney Area Health Service (CSAHS) of Sydney comprises several hospitals, community care centres and nursing homes. A questionnaire was administered to one fifth of all of the employees of the CSAHS, investigating their attitudes, beliefs and knowledge about HIV/AIDS. 1,056 responses were obtained. The dat
Health strategies of HIV-infected, homosexually-active men in Sydney, Australia: implications for health care workers.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:81 (poster no. 16). Unique Identifier : AIDSLINE ASHM5/94349048; Ariss R; Carriqan T; Dowsett G; National Centre for HIV Social Research, Macquarie University,; Sydney, Australia.; AIM: To map the socio/cultural determinants of the health strategies adopted by HIV positive, homosexually-active men. METHOD: From an existing cohort of 535 men (Social Aspects of the Prevention of AIDS, 1987), 10 HIV positive homosexually-active men were selected to explore responses to HIV infection. The study empl
Design and implementation of a program to educate doctors and health care providers about living with HIV/AIDS by people living with HIV/AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:82 (poster no. 17). Unique Identifier : AIDSLINE ASHM5/94349049; Hillier J; McKenzie J; Victorian AIDS Council, Collingwood, Aus.; OBJECTIVES: To increase the awareness of doctors and health care providers about living with HIV/AIDS through the personal experiences of People Living With HIV/AIDS. METHODS: People with HIV/AIDS discuss their personal experiences with groups of doctors and other health care providers to convey appropriate and inappr
HIV/AIDS grief, loss & bereavement: community response.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:83 (poster no. 18). Unique Identifier : AIDSLINE ASHM5/94349050; Raphael B; Woolcock G; OBJECTIVE: To improve understanding and knowledge of the social and psychological aspects of grief and loss associated with HIV/AIDS within AIDS organisations. METHOD: One of the recommendations from a National Centre HIV/AIDS grief and loss colloquium was to review policies and programs within AIDS organisations with
Psychoimmunology and the Wellness Programme.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:84 (poster no. 20). Unique Identifier : AIDSLINE ASHM5/94349051; Rayner-Brosnan DP; At the National Centre for HIV Social Research and within The Wellness Programme, a model has been developed that points to specific psychobehavioural factors for the HIV positive person that have immune consequences. A prospective longitudinal study is now underway to increase current knowledge about those aspects wi
Contexts of unsafe sex among non-homosexually-identified men.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:85 (poster no. 21). Unique Identifier : AIDSLINE ASHM5/94349052; Crawford J; Prestage G; Hood D; Kippax S; Sorrell T; National Centre for HIV Social Research, Macquarie Unit,; Macquarie University, NSW.; OBJECTIVES: Identification of some contextual factors associated with unprotected anal intercourse. METHOD AND SAMPLE: Two volunteer samples were interviewed by telephone. One (Project Male-Call) in May-June 1992 with 2583 men nationwide who had had male-to-male sex during the preceding five years. A further 3906 non
Sentinel HIV surveillance in STD Clinics in Australia. Collaborative Group on Sentinel HIV Surveillance in STD Clinics.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:85 (poster no. 22). Unique Identifier : AIDSLINE ASHM5/94349053; McDonald A; Kaldor J; National Centre in HIV Epidemiology and Clinical Research,; Sydney, Australia.; OBJECTIVE: To describe the pattern of HIV antibody testing and newly diagnosed HIV infection among people seen at public sexually transmissible disease (STD) clinics in Australia . METHODS: Each quarter, six STD clinics forwarded tabulations of the number of people seen, the numb
Epidemiology of Qld HIV and AIDS patients.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:86 (poster no. 23). Unique Identifier : AIDSLINE ASHM5/94349054; Magon H; Ree GH; Kennedy J; Cornell V; AIDS Medical Unit, Brisbane.; Long term patient HIV data was divided into 3 groups based on their current CD4 count. The results survey the epidemiological differences between groups and focus on differences between current and initial CD4 levels. Little data has been published on CD4 counts. Surveying Qld patients diagnosed with AIDS post 1/1/89
High risk sexual behaviour of socio-economically disadvantaged youth.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:86 (poster no. 24). Unique Identifier : AIDSLINE ASHM5/94349055; Donald M; National Centre for HIV Social Research, Royal Brisbane Hospital,; Herston, Qld.; Poverty and culture are important determinants of condom use and socio-economic factors are linked to STDs in adolescents. The aim of the study is to investigate disadvantaged youths risk for HIV, STDs and unwanted pregnancies and to ascertain the critical behavioural and psychosocial determinants of high risk sexual
Epidemiology of HIV infection seen at the Royal Adelaide Hospital, 1985-1993.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:87 (poster no. 26). Unique Identifier : AIDSLINE ASHM5/94349056; Wells R; Waddell R; Shaw D; Department of Infectious Diseases, Royal Adelaide Hospital, North; Terrace, SA.; Between 1985 and May 1993, 297 patients infected with HIV have been treated by the infectious diseases department of the Royal Adelaide Hospital, 110 patients had AIDS defining illnesses and 75 died. The characteristics of this population were studied, including age, degree of immunosuppression at presentation to the
Provision of needles and syringes to injecting drug users (IDU) from pharmacies in Victoria.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:87 (poster no. 25). Unique Identifier : AIDSLINE ASHM5/94349057; Mathews G; Batchelor M; Carswell P; Marcon N; Department of Health and Community Services Public Health Branch,; Health Protection, AIDS/STD Unit, Melbourne, Victoria.; Both the Victorian Needle and Syringe Exchange Program (NSEP) and Pharmacy Program provide sterile injecting equipment to Injecting Drug Users (IDU). The broad aim of the program is to prevent the transmission of HIV amongst IDU and from IDU to the broader community. Although the NSEP has been successful in reaching a
Lifestyles and sexual behaviours of non gay attached homosexually active working class men in Western Sydney.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:88 (poster no. 28). Unique Identifier : AIDSLINE ASHM5/94349058; Hood D; Millan G; O'Reilly C; Ramsay J; Western Sydney AIDS Unit, Parramatta Health Service Cnr, NSW.; Western Sydney is the largest sector of the city of Sydney comprising an extensive industrial base and dormitory suburbs of 1.3 million people of diverse socio-economic status. The 1989 Western Sydney Beats Study established that there was an active network of illicit public sex venues known as beats . In that survey6
Modelling the HIV epidemic, results from a three year project.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:88 (poster no. 27). Unique Identifier : AIDSLINE ASHM5/94349059; Kault D; Dept. Maths & Stats, James Cook University, Townsville, Qld.; HIV spreads through a network of sexual contacts. Sexual behaviour surveys give insufficient information about this network to allow predictions of the spread of HIV. However, this information is encoded in the detailed epidemiology of other STDs as these have spread through the same network now available for HIV. Mat6
Human immunodeficiency virus antibody testing at the Gay Men's Health Centre. Melbourne.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:89 (poster no. 29). Unique Identifier : AIDSLINE ASHM5/94349060; Carmody C; Royal Prince Alfred Hospital, Camperdown, Sydney.; BACKGROUND: The Gay Men s Health Centre is a significant testing centre for gay men in Victoria, requesting over 600 Human Immunodeficiency Virus (HIV) antibody tests annually. OBJECTIVES: To determine the number of new diagnoses of HIV infection at the Centre and to obtain epidemiological information on those individ6
HIV antibody testing and HIV risk behaviour among clients attending methadone clinics in NSW.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:89 (poster no. 30). Unique Identifier : AIDSLINE ASHM5/94349061; MacDonald M; Elford J; Wodak A; Ward P; Kaldor J; National Centre in HIV Epidemiology and Clinical Research,; Darlinghurst, NSW.; OBJECTIVE: To describe HIV antibody testing policy and practice, and information collected concerning HIV risk behaviour among clients attending methadone clinics in NSW. METHODS: In April 1993, questionnaires concerning HIV testing policy and practice, and the information collected on HIV risk behaviour, were sent to6
Factitious AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:90 (poster no. 31a). Unique Identifier : AIDSLINE ASHM5/94349062; Munckhof WJ; Jenkins K; Fairfield Hospital, Vic.; A 19 year old homosexual man was admitted to a general hospital with 3 weeks sweats and cough with haemoptysis. He stated that HIV serology performed one year earlier was positive, and claimed to have pulmonary tuberculosis treated with isoniazid. Physical examination and chest X-ray were normal. Mantoux test was nega6
Trend analysis & back-protection analysis of Qld HIV data.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:90 (poster no. 31). Unique Identifier : AIDSLINE ASHM5/94349063; Magon H; Ree GH; Kennedy J; Cornell V; AIDS Medical Unit, Brisbane.; Two widely popular methods for predicting the future incidence of AIDS cases were applied to the Queensland HIV database. Trend analysis gave high to low estimates of AIDS cases & deaths. Estimated new AIDS cases over the three year period 1993 to 1995 ranged from 258 to 348 with AIDS deaths ranging from 184 to 276
Cytomegalovirus in prostates of AIDS patients.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:91 (poster no. 32). Unique Identifier : AIDSLINE ASHM5/94349064; Katelaris A; NSW Institute of Forensic Medicine, Glebe.; The incidence of Cytomegalovirus ( CMV ) infection in the prostates of HIV positive patients was investigated. HIV positive patients were autopsied at the NSW Institute of Forensic Medicine. Prostatic tissue samples were studied by H&E staining and with CMV specif
Relapsing Helicobacter cinaedi bacteremia in an AIDS patient.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:91 (poster no. 33). Unique Identifier : AIDSLINE ASHM5/94349065; Munckhof WJ; Street AC; Tee W; Fairfield Hospital, Vic.; Helicobacter cinaedi is a rare cause of gastroenteritis and bacteremia occurring most commonly in immunocompromised patients. We report a case of Helicobacter cinaedi bacteremia in an AIDS patient that relapsed despite a course of adequate antibiotic treatment. A 33 year old patient with AIDS diagnosed 1 year previous
How useful is colonoscopy in the management of diarrhoea in HIV infected patents.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:92 (poster no. 34). Unique Identifier : AIDSLINE ASHM5/94349066; Baingana S; Hoy J; Brown G; Department of Medicine, Royal Melbourne Hospital, University of; Melbourne.; AIM: To determine the prevalence of organisms isolated, the role of colonoscopy and/or endoscopy in diagnosis, response to therapy and relation of patient s immunological and nutritional status to outcome in HIV infected individuals managed at Fairfield Hospital. DESIGN AND METHODOLOGY: Retrospective, Case Series, Con
HIV infection in haemophilia.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:92 (poster no. 35). Unique Identifier : AIDSLINE ASHM5/94349067; Chan T; Street A; Haemophilia Treatment Centre, Alfred Hospital, Prahran, Vic.; HIV infection in haemophilia differs in several respects from infection in other groups. Co-infection with Hepatitis C (HCV) and/or Hepatitis B (HBV) is common and its course may be modified by HIV. HIV may also alter the natural history of haemophilia. This paper presents data on 75 evaluable persons with haemophilia
Greater quinolinic acid production by macrophages infected with demented versus non-demented isolates of HIV.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:93 (poster no. 37). Unique Identifier : AIDSLINE ASHM5/94349068; Brew BJ; Pemberton L; Evans L; Heves M; Centre for Immunology, St Vincent's Hospital, Sydney, Australia.; OBJECTIVES: We have previously reported that Quinolinic acid (QUIN), a neurotoxin acting through the N Methyl D Aspartate receptor, is markedly elevated in the cerebrospinal fluid (CSF) of patients with AIDS dementia complex (ADC) and is produced by HIV-1 infected and gamma interfe
Induction of pro-inflammatory cytokines by Cryptococcus neoformans.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:93 (poster no. 36). Unique Identifier : AIDSLINE ASHM5/94349069; Visvanathan K; Jones PD; Department of Infectious Diseases, Prince Henry Hospital, Sydney,; Australia.; AIM: To characterise the induction of proinflammatory cytokines by C. neoformans and to determine if complement is an essential factor in this induction. METHODS: Peripheral blood mononuclear cells (PBM) were obtained from normal hosts and stimulated in vitro with either killed, whole C. neoformans or lipopolysacchari
Increase in CD4 lymphocyte numbers after splenectomy in patients infected with the human immunodeficiency virus.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:94 (poster no. 38). Unique Identifier : AIDSLINE ASHM5/94349070; Fairley CK; Spelman D; Street A; Jennens ID; Spicer WJ; Crowe S; Department of Social and Preventive Medicine, Alfred Hospital,; Prahran, Victoria.; The effect of splenectomy on lymphocyte subsets was assessed by retrospectively examining the charts of 10 patients who were infected with HIV and who had undergone splenectomy. There was a significant increase in the mean CD4 numbers following splenectomy (mean increase of 326/microliters, P = 0.009) the total lympho
Mycobacterium avium complex infections (MAC) in AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:94 (poster no. 39). Unique Identifier : AIDSLINE ASHM5/94349071; Fuller A; Spellman D; Spicer WJ; Department of Microbiology and Infectious Diseases, Alfred; Hospital, Prahran, Vic.; STUDY OBJECTIVES: We chose to assess the prevalence and impact of MAC on patients with AIDS attending the hospital. METHODS: Retrospectively we identified 62 HIV patients with positive MAC cultures and reviewed their hospital files. Our analysis included whether MAC was the AIDS defining illness, the CD4 count, alkali
Implications of the 1993 revised AIDS case definition on a Victorian population of HIV infected individuals.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:95 (poster no. 40). Unique Identifier : AIDSLINE ASHM5/94349072; Greenhalgh A; Stewart T; Hoy J; Crowe S; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; Effective from January 1, 1993, the US Centre for Disease Control (CDC), expanded the AIDS case definition to include all adults and adolescents infected with HIV with either an absolute number of peripheral CD4+ T cells
Yield from upper endoscopic gastrointestinal biopsy of macroscopically normal mucosa in AIDS patients.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:95 (poster no. 41). Unique Identifier : AIDSLINE ASHM5/94349073; Kelly M; Engelman J; Lloyd A; Jones P; Pirola R; Department of Infectious Diseases, Prince Henry Hospital, Little; Bay, Sydney.; AIM: To review the diagnostic yield from the biopsy of macroscopically normal mucosa seen at upper GIT endoscopy in AIDS patients with GIT symptoms. METHOD: The case records of 62 AIDS patients having upper GIT endoscopies between 6/91 and 6/93 were retrospectively reviewed. Clinical history; endoscopic appearance; hi
Pseudopancreatitis in HIV disease.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:96 (poster no. 43). Unique Identifier : AIDSLINE ASHM5/94349074; Parkin D; Edwards R; Department of HIV Medicine, Royal North Shore Hospital, St; Leonards, NSW.; We report a case of hyperamylasemia in a 39 year old AIDS patient. He presented in May 1992 with Pneumocystis carinii pneumonia and was subsequently found to be HIV antibody positive. His CD4 Count was 32. On recovery AZT 600mg a day and Bactrim were
Kaposi's sarcoma (K.S.): the Fairfield Hospital experience.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:96 (poster no. 42). Unique Identifier : AIDSLINE ASHM5/94349075; Padiglione A; Goldstein D; Fairfield Hospital, Vic.; Kaposi s sarcoma is the most common malignancy occurring in patients with HIV infection, affecting about 21% of patients with AIDS, and is associated with significant morbidity and mortality. A number of therapeutic interventions are employed in the routine management of these patients, principally being alpha interfe
Management of extensive oral Kaposi's sarcoma.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:97 (poster no. 44). Unique Identifier : AIDSLINE ASHM5/94349076; Sherson W; St Vincents Medical Centre, Darlinghurst, N.S.W.; A 29 year old male homosexual with stage IV HIV infection presented with extensive bilateral KS involving the hard palate and occluding the palatal airspace. Clinical management involved a combined therapy approach including chemotherapy (vincristine bleomycin), surgical de-bulking, radiotherapy and electrocautery ove
Necrotising ulcerative stomatitis in association with necrotising ulcerative periodontitis in a HIV infected male.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:97 (poster no. 45). Unique Identifier : AIDSLINE ASHM5/94349077; Coates E; Gorkic E; Rogers G; Goss AN; Restorative Unit, Adelaide Dental Hospital, S.A.; A 21-year-old Caucasian male presented with acute and severe necrotising oral infection. He had been diagnosed HIV positive two months previously. His CD4 count was 726, and he was not taking any medication related to his HIV infection. He was a homosexual IV drug user. Immediate Antibiotic therapy was instituted with
An unusual case of AIDS.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:98 (poster no. 47). Unique Identifier : AIDSLINE ASHM5/94349078; Padiglione A; Fairfield Hospital, Vic.; In early 1993 an elderly Vietnamese lady presented to Fairfield Hospital with skin lesions, fever and was found to have disseminated MAC and cryptococcal meningitis. Subsequent tests revealed a profound suppression of lymphocytes, with a CD4 count of 270 and CD8 count of 80. Despite treatment and clinical improvement
CMV ileitis in an AIDS patient.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:98 (poster no. 46). Unique Identifier : AIDSLINE ASHM5/94349079; Hale G; Fuller A; Woolley I; Department of Microbiology and Infectious Diseases, Alfred; Hospital, Prahran.; We describe the case of an Asian man with AIDS who was shown to have CMV as the cause of his severe small bowel disease. The diagnosis was made by finding CMV inclusions in his terminal ileum. He responded well to a three week course of ganciclovir and then rela
In vitro studies of Schwann cell infectivity by human immunodeficiency virus (HIV).: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:99 (poster no. 48). Unique Identifier : AIDSLINE ASHM5/94349080; Ayers MM; Turnbull V; Siregar H; Crowe SM; Gonzales MF; Neuropathology Research Laboratory, Royal Melbourne Hospital,; Parkville, Vic.; Attention has recently focussed on Schwann cells as antigen presenting cells in inflammatory demyelinating polyneuropathy (IDPN). Whether Schwann cells process and present antigen following viral infection is of particular interest. To determine if human Schwann cells can be infected by HIV, cultures of Schwann cells
Measurement of plasma viraemia in HIV infected individuals using CD4 capture and RT-PCR.: Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:99 (poster no. 49). Unique Identifier : AIDSLINE ASHM5/94349081; Block AA; Sonza S; Dunne AL; Mills J; Crowe SM; Macfarlane Burnet Centre for Medical Research, Fairfield,; Victoria.; The use of reverse transcription and the polymerase chain reaction (RT-PCR) has become an integral part of the detection of viral RNA levels in the plasma of HIV infected individuals. The high sensitivity of this detection system makes it particularly useful in the monitoring of anti-retroviral therapy. Previous studi
Differences in immune function among patients infected with distinct Thailand HIV-1 strains.: Int Conf AIDS. 1994 Aug 7-12;10(1):10 (abstract no. 012C). Unique Identifier : AIDSLINE ICA10/94369240; Weniger BG; Tansuphaswadikul S; Young NL; Pau CP; Lohsomboon P; Yindeeyoungyeon W; Limpakarnjanarat K; Division of HIV/AIDS (E-50), CDC, Atlanta, Georgia 30333.; INTRODUCTION: Thai HIV-1 genotype A/subtype E and genotype B/subtype B circulate widely in Thailand , with A spread most often by sex and B predominant among IDUs. METHODS: Among symptomatic HIV+ Bangkok outpatients (n = 60) and inpatients (n = 267) from 8/91-9/92, 216 A and 73 B
Domain between V1 and V2 regions of HIV-1 gp120 is critical for virus infectivity.: Int Conf AIDS. 1994 Aug 7-12;10(1):10 (abstract no. 014A). Unique Identifier : AIDSLINE ICA10/94369241; Wang WK; Essex M; Lee TH; Department of Cancer Biology, Harvard School of Public Health,; Boston, MA 02115.; OBJECTIVE: Between the first and second variable regions (V1 and V2) of HIV-1 gp120 is a conserved domain containing a relatively large number of charged residues. These residues were investigated for their role in viral infectivity. METHODS: Mutant proviruses each with an alanine substituted for one of 9 highly conse
Mechanisms of HIV-1 envelope glycoprotein-mediated membrane fusion.: Int Conf AIDS. 1994 Aug 7-12;10(1):10 (abstract no. 015A). Unique Identifier : AIDSLINE ICA10/94369242; Dimitrov DS; Golding H; Broder CC; Blumenthal R; National Cancer Institute, NIH, Bethesda, MD 20892.; OBJECTIVE: To delineate factors affecting the kinetics of membrane fusion mediated by the HIV-1 envelope glycoprotein (gp120-gp41 complex). METHODS: Kinetics of cell-cell and virus-cell membrane fusion was measured by assays based on redistribution of fluorescent dyes, photoinactivation, syncytia formation and infecti
Restricted replication of SIVMAC239 in macrophages.: Int Conf AIDS. 1994 Aug 7-12;10(1):10 (abstract no. 013A). Unique Identifier : AIDSLINE ICA10/94369243; Mori K; Desrosiers R; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA.; An infectious, pathogenic, molecular clone of SIVmac239 causes AIDS and death in rhesus monkeys in a time frame suitable for laboratory investigation. This cloned SIVmac239 replicates poorly in primary macrophage cultures from rhesus monkeys. Variants with high replicative capacity for macrophages emerge in about 40%
Functional analysis of HIV-1 glycoproteins with deletions in gp120.: Int Conf AIDS. 1994 Aug 7-12;10(1):100 (abstract no. PA0020). Unique Identifier : AIDSLINE ICA10/94369244; Adams O; Schaal H; Scheid A; Universitat Dusseldorf, Germany.; OBJECTIVES: (1) To determine the contribution of gp120 to the membrane fusion activity of the HIV-1 glycoprotein and (2) to determine the structured requirements for HIV-1 glycoprotein oligomerization. METHODS: A vector was constructed which expresses the HIV-1 env, tat, and rev genes under the control of the SV40 ear
Gp120 induces CD4-association with membrane component(s) and susceptibility to PMA-induced co-modulation.: Int Conf AIDS. 1994 Aug 7-12;10(1):100 (abstract no. PA0017). Unique Identifier : AIDSLINE ICA10/94369245; Golding H; Lapham C; CBER, FDA, Bethesda, MD 20892.; OBJECTIVE: To identify additional membrane components required for HIV-1 entry and envelope mediated fusion (CD4-co-receptor). METHODS: Using cell lines expressing either tailless CD4 molecules (CD4.401) or chimeric CD4.CD8 molecules, we found that PMA does not induce down-modulation of these receptors. Yet PMA treatm
Laminin and fibronectin interfere with the binding of HIV-1 gp120/160 to CD4.: Int Conf AIDS. 1994 Aug 7-12;10(1):100 (abstract no. PA0018). Unique Identifier : AIDSLINE ICA10/94369246; Bozzini S; Falcone V; Conaldi PG; Visai L; Dolei A; Speziale P; Toniolo A; Dept. of Biochemistry, University of Pavia, Italy.; OBJECTIVE: In vitro experiments indicate that not yet defined host components may hinder the early interactions of HIV-1 with target cells. We checked whether human extracellular matrix proteins could bind HIV-1 env glycoproteins thus preventing virus-receptor interaction, or facilitating infection by trapping the vir
Possible role of HIV gp120 in CD2/LFA-3 activation pathway.: Int Conf AIDS. 1994 Aug 7-12;10(1):100 (abstract no. PA0019). Unique Identifier : AIDSLINE ICA10/94369247; Blinov VM; Resenchuk SM; Denisov SI; Chirikova GB; Zverev VV; Institute of Molecular Biology, Koltsovo, Novosibirsk, Russia.; The CD2 molecule belongs to a family of cell-surface glycoproteins that function as adhesion molecules in the immune system. Human CD2 is found exclusively on cells of the T lineage: peripheral T lymphocytes, NK cells, and thymocytes. CD2 binds specifically to the surface glycoprotein LFA-3. More importantly, CD2/LFA-
HIV-infected cells can kill CD4+ cells upon contact.: Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0023). Unique Identifier : AIDSLINE ICA10/94369248; Nardelli B; Gonzalez CJ; Schechter M; Valentine FT; Department of Medicine, NYU Medical Center, NY 10016.; OBJECTIVE: To explore an additional phenomenon by which HIV-1-infected cells may cause the depletion of CD4+ lymphocytes in vivo. METHODS: Stimulated PBMC from seronegative donors were infected with HIV-1 clinical isolates. After several days in culture, infected PBMC or CD4+ lymphocytes were incubated with autologous
Peptidase activity of CD26 is not necessary for its function in HIV entry and cytopathic effect.: Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0022). Unique Identifier : AIDSLINE ICA10/94369249; Callebaut C; Krust B; Marie I; Jacotot E; Robert N; Guichard G; Briand JP; Muller S; Barth A; Montagnier L; et al; Unite de Virologie & Immunologie Cellulaire, Institut Pasteur,; Paris, France.; CD26, also known as dipeptidyl peptidase IV (DPP IV), serves as a coreceptor of CD4 for entry of HIV into permissive cells. In the infected cell, CD26 is involved along CD4 in the mechanism of the characteristic cytopathic effect (CPE) of HIV which is triggered by the membrane expressed HIV envelope glycoproteins. CD2
Possible cooperativity of tryptase TL2 and CD26 on proteolytic cleavage of V3 loop of HIV-1 gp120.: Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0021). Unique Identifier : AIDSLINE ICA10/94369250; Niwa Y; Ohkubo I; Kido H; Inst. for Enzyme Res., Univ. of Tokushima, Japan.; OBJECTIVES: Infection by HIV-1 is due to virus-cell fusion mediated by the viral envelope glycoproteins gp120 and gp41 with CD4 and the other cellular cofactors. In this study, possible cooperativity of cellular cofactors, such as tryptase TL2 and CD26, on proteolytic cleavage of V3 loop of HIV-1 gp120 was analyzed. M
Unessential of calcium ions and inhibitor specificity for the intracellular gp160 processing.: Int Conf AIDS. 1994 Aug 7-12;10(1):101 (abstract no. PA0024). Unique Identifier : AIDSLINE ICA10/94369251; Okumura Y; Kamoshita K; Koga Y; Sasaki M; Kido H; Inst. for Enz. Res., Univ. of Tokushima, Japan.; OBJECTIVES: Endoproteolytic cleavage of the envelope glycoprotein precursor (gp160) of HIV-1 by cellular protease(s) is required for the full activity of virus. In this study, the requirement of calcium ions and the inhibitor specificity for the intracellular gp160 processing were analyzed. METHOD: Intracellular gp160
Identification of functional domains of HIV-1 integrase.: Int Conf AIDS. 1994 Aug 7-12;10(1):102 (abstract no. PA0026). Unique Identifier : AIDSLINE ICA10/94369252; Kitamura Y; Coffin JM; Division of Molecular Genetics, National Institute of Health,; Tokyo, Japan.; Integration of a DNA copy of the viral RNA genome into host cellular DNA is essential and unique to the retroviral life cycle. Following reverse transcription, the 3 ends of the linear viral DNA are cleaved to expose the CA-OH ends (processing reaction) that become joined to the target DNA (strand transfer reaction).
Inhibitory mechanism of camptothecin on HIV-1 replication.: Int Conf AIDS. 1994 Aug 7-12;10(1):102 (abstract no. PA0028). Unique Identifier : AIDSLINE ICA10/94369253; Takahashi H; Kojima A; Kurata T; Dep. of Pathology, National Institute of Health, Tokyo, Japan.; OBJECTIVES--Camptothecin (CPT), a topoisomerase I inhibitor was reported to block retroviral infection in vitro and in vivo. CPT is also known as an antitumor drug and is toxic to eukaryotic cells. As HIV-1 replicates very poorly in non-dividing cells, CPT inhibits HIV possibly by suppressing cell growth, otherwise it
Unintegrated HIV-specific DNA in plasma of infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):102 (abstract no. PA0027). Unique Identifier : AIDSLINE ICA10/94369254; Nandi J; Bhavalkar V; Kamat H; Banerjee K; National Inst. of Virology, Pune, India.; OBJECTIVE: The study was undertaken to detect HIV-specific DNA in plasma of infected individuals and compare it with the other nucleic acid markers of the virus i.e. HIV-specific DNA in PBL as the provirus and HIV-specific RNA, in the plasma of infected persons. METHODS: Blood samples were collected from asymptomatic,
Delay of emergence of ddI resistant specific mutation after long term therapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0031). Unique Identifier : AIDSLINE ICA10/94369255; Kondo M; Saito T; Hayashi T; Ito A; Nagao T; Oka S; Imai M; Kanagawa Prefectural Institute of Health, Japan.; OBJECTIVES: To study emergence of drug resistance specific mutation after ddI therapy and to study the effect of AZT therapy before ddI therapy on the emergence of the mutation. METHODS: HIV-1 strains were isolated from a patient treated with ddI and
HIV-1 production from unintegrated provirus in transfected Jurkat clones.: Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0030). Unique Identifier : AIDSLINE ICA10/94369256; Gurgo C; Zella D; Reitz MS Jr; Gallo RC; Laboratory of Tumor Cell Biology, NCI NIH, Bethesda, MD.; Production of viral antigens and infectious virus by Jurkat cells transfected with pHXB2 (nef+) progressively declined over an extended period of time in culture. Sixty clones were generated from the transfected line; they were analyzed for the presence of provirus and monitored for the expression of viral antigens (s
LTR-directed homologous recombination of HIV-1 proviral clone in recA(-) bacteria.: Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0029). Unique Identifier : AIDSLINE ICA10/94369257; Yamada K; Nakano T; Okamoto T; Dept. Mol. Genetics, Nagoya City Univ. Med. School, Aichi, Japan.; OBJECTIVE: In this study we have attempted to clarify the genetic mechanism of frequent homologous recombination (HR) during cloning of a full length HIV-1 plasmid in recA(-) host bacteria. METHODS: A plasmid containing the full length HIV-1 sequence, pNL432, was transfected into various recA(-) bacterial strains. The
The microtiter point mutation assay to detect drug resistance in HIV-seropositive patients treated with AZT.: Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0032). Unique Identifier : AIDSLINE ICA10/94369258; van der Feltz M; de Haas C; de Graaf L; Borleffs JC; Visser MR; Verhoef J; University Hospital, Dept. of Internal Medicine, Immunology and; Inf. Dis., Utrecht, The Netherlands.; BACKGROUND: After prolonged therapy of HIV-seropositive patients with zidovudine ( AZT ), drug resistant virus strains emerge. Five point mutations of the viral reverse transcriptase are responsible for various degrees of drug resistance. A rapid, simpl
Detection of reverse transcriptase (RT) codon 215 mutations by PCR in HIV-infected adults: correlation with clinical disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):104 (abstract no. PA0033). Unique Identifier : AIDSLINE ICA10/94369259; Nelson R; Bradley W; Day N; Good R; Univ. South Florida/All Children's Hospital, St. Petersburg; 33701.; OBJECTIVE: To rapidly detect mutations of the HIV-1 reverse transcriptase codon 215 (associated with azidothymidine resistance) from peripheral blood DNA of disease-progressing and non-progressing adult patients. METHODS: Thirty-two peripheral blood mononuclear cell samples were obtained from 29 HIV-infected adults. C
Distinct mechanisms of HIV-1 latency in the two cell line models U1 and ACH-2.: Int Conf AIDS. 1994 Aug 7-12;10(1):104 (abstract no. PA0035). Unique Identifier : AIDSLINE ICA10/94369260; Cannon P; Kim SH; Ulich C; Kim S; Seoul National University, Korea.; The U1 and ACH-2 cell lines are human monocytic and T-lymphoid cells respectively, persistently infected with HIV-1. These cell lines express viral RNA and proteins at very low levels, but can be induced to produce larger amounts virion particles. For this reason, they have been used extensively as in vitro models of
HIV LTR drives gene expression in mouse and human eggs.: Int Conf AIDS. 1994 Aug 7-12;10(1):104 (abstract no. PA0034). Unique Identifier : AIDSLINE ICA10/94369261; Kiessling A; Crowell R; Michalopoulos J; Waldorf T; Deaconess/Harvard Surgical Service, Faulkner Hospital, Boston, MA; 02130.; OBJECTIVE: To determine if fertilized eggs would express HIV proteins. METHODS: Mouse zygotes and human polypronuclear eggs were microinjected with DNA constructs containing HIV-LTR linked to lacZ reporter gene. Mouse zygotes were microinjected with 2 to 10,000 copies of plasmid pHIV-LTR, cultured for 48 hours, fixed
Study of differential RNA expression during HIV infection using arbitrarily primed PCR.: Int Conf AIDS. 1994 Aug 7-12;10(1):104 (abstract no. PA0036). Unique Identifier : AIDSLINE ICA10/94369262; Boyer V; Ferre F; Pezzoli P; Jensen FC; Carlo DJ; Immune Response Corporation, Carlsbad CA.; OBJECTIVE: To identify differential RNA expression during HIV infection of a T cell line (HUT78 cell line), we used the RNA arbitrarily primed PCR (RAP) fingerprinting method. METHODS: Total RNA was isolated from HUT78 cells and HIV-1 (HIVZ321 strain)-infected HUT78 cells. cDNA was generated using an arbitrarily selec
A new method of selecting HIV inhibitor in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0038). Unique Identifier : AIDSLINE ICA10/94369263; Cai C; Koga Y; Nomoto K; Medical Institute of Bioregulation, Kyushu University, Fukuoka,; Japan.; OBJECTIVE: A safe and rapid method of selecting HIV inhibitor in vitro is necessary for AIDS research and therapy. We established the method on HIV-1 env gene expression cell lines. METHODS: Human CD4+ T and monocytoid cell lines were transferred with HIV-1 env gene expression vector in which the activity of the promo
Identification of EBV-transformed lymphocytes in HIV-infected lymphnodes.: Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0040). Unique Identifier : AIDSLINE ICA10/94369264; Zheng H; Mori S; Dept. of Pathol., Univ. of Tokyo, Japan.; OBJECTIVE: Most of AIDS-related lymphomas (AIDS-ML) are infected with EBV. In order to investigate the origin of these neoplastic cells, we made an analysis using molecular biologic methods. METHOD: We examined 35 cases of HIV-infected lymphnodes that were in PGL stage. For the demonstration of EBERs, we used in situ
Regulation of HIV-1 gene expression in monocyte-derived macrophages.: Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0039). Unique Identifier : AIDSLINE ICA10/94369265; Moelans I; de Vos M; de Graaf L; Nottet H; Visser MR; Verhoef J; Eijkman-Winkler Institute of Medical Microbiology, Utrecht, The; Netherlands.; OBJECTIVE: N-acetyl-L-cysteine (NAC), phorbol 12-myristate (PMA) and lipopolysaccharide (LPS), have an opposite effect on HIV-1 production in monocyte-derived macrophages (MDM) when compared with their effect in lymphocytes. We have investigated if the different effects in lymphocytes and MDM are due to a difference i
Role of HIV as cofactor in HPV oncogenesis.: Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0037). Unique Identifier : AIDSLINE ICA10/94369266; Tornesello ML; Buonaguro FM; Del Gaudio E; Buonaguro L; Beth-Giraldo E; Giraldo G; Div. of Viral Oncology, Ist. Naz. Tumori Fond. Pascale, Naples,; Italy.; OBJECTIVE: The increased incidence in AIDS patients of Human Papillomavirus (HPV) associated lesions stimulated our studies to determine the role of HIV on HPV and to identify the molecular mechanisms involved in such interaction. We have previously shown that HIV-1 Tat transactivates the HPV16 Long Control Region (LC
Analysis of pol gene of a non-infectious HIV-1 clone.: Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0043). Unique Identifier : AIDSLINE ICA10/94369267; Nakano T; Sano K; Goto T; Morimatsu S; Ueda S; Ikuta K; Kato S; Nakai M; Department of Microbiology, Osaka Medical College, Japan.; OBJECTIVE: We analyzed the nucleotide sequence of pol gene encoding protease (PR) in a non-infectious HIV-1 producing cell clone L-2, and examined the expression of pol gene products. METHODS: Conventional dideoxy-termination method was used to sequence PR coding region of L-2 and Molt-4/LAV-1 cells. The expressions o
Regulation of HIV by retinoic acid (RA) correlates with expression of RA receptors.: Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0042). Unique Identifier : AIDSLINE ICA10/94369268; Yamaguchi K; Groopman JE; Byrn RA; Division of Hematology/Oncology, New England Deaconess Hospital,; Bostron, MA 02215.; OBJECTIVE: Analyze the effect of retinoic acids (RA) on human immunodeficiency virus type 1 (HIV-1) expression and correlate this effect with levels of retinoic acid receptor expression in T-lymphoid and monocytoid cell lines. METHODS: The effect of all-trans retinoic acid and 9-cis retinoic acid on HIV-1 production i
Role of natural suppressor glutamine tRNA on growth control of retroviruses.: Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0041). Unique Identifier : AIDSLINE ICA10/94369269; Kuchino Y; Muller WE; Odawara T; Yoshikura H; Biophys. Div., National Cancer Center Res. Inst., Tokyo, Japan.; Frameshift suppression or nonsense suppression has a crucial role in expression regulation of retrovirus genomic informations. Recently, we isolated a natural suppressor tRNA from human and rodent cells, which recognizes the UAG amber codon as a glutamine codon in an in vitro translation system prepared from a rabbit
The C-terminal domain of HIV-1 Vpr causes cell growth arrest and structural defects.: Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0044). Unique Identifier : AIDSLINE ICA10/94369270; Macreadie I; Castelli L; Hewish D; Ward A; Azad A; Biomolecular Research Institute, Melbourne, Australia.; OBJECTIVE: To determine possible role of HIV-1 Vpr, a virion-associated accessory protein, in AIDS pathogenesis. METHODS: Full length and truncated vpr genes were expressed in yeast and the effects of expression on cell growth and morphology was studied. The effects of electroporation of synthetic C-terminal peptides
Cellular factor that binds to the leucine-rich motifs of HIV-1 Rev and HTLV-I Rex proteins.: Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0048). Unique Identifier : AIDSLINE ICA10/94369271; Kubota S; Adachi Y; Copeland TD; Oroszlan S; ABL-Basic Research Program, NCI-FCRDC, MD.; OBJECTIVE: HIV-I Rev protein and HTLV-I Rex protein possess their own conserved leucine-rich domains which are thought to interact with cellular factors. Our aim has been to isolate and characterize these co-factors via biochemical approaches. METHODS: Oligopeptides corresponding to the motifs of Rev (EPVPLQLPPLERLTLD
HIV-1 Vpu liberates Golgi-targeted HIV-1 env gp160 from CD4-dependent retention in the endoplasmic reticulum.: Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0045). Unique Identifier : AIDSLINE ICA10/94369272; Nishikawa M; Kimura T; Department of Microbiology, Kansai Medical University, Osaka,; Japan.; OBJECTIVE: The experiments were aimed to analyse the effects of HIV-1 Vpu on subcellular localization and processing of HIV-1 env gp160 that had been complexed and retained with CD4 molecules in the Endoplasmic reticulum (ER). METHODS: COS-1 cells were transfected to express HIV-1 env, vpu and rev, and CD4 genes. Anal
Requirement of host factors for HIV-1 Rev function.: Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0047). Unique Identifier : AIDSLINE ICA10/94369273; Shukla RR; Marquis SM; Kimmel PL; Kumar A; Department of Biochemistry & Molecular Biology, George Washington; University, Washington DC 20037.; The nucleocytoplasmic transport of unspliced or singly spliced viral mRNA transcripts is required for the expression of Human immunodeficiency virus type 1 structural proteins. HIV-1 regulatory protein Rev binds to Rev Responsive Element (RRE) in the Env encoding region of the viral mRNA and facilitates the transport
Study of HIV1 Tat transactivation mechanism by using synthetic Tat protein and Tat peptides.: Int Conf AIDS. 1994 Aug 7-12;10(1):107 (abstract no. PA0046). Unique Identifier : AIDSLINE ICA10/94369274; Vives E; Charneau P; van Rietschoten J; Rochat H; Bahraoui E; CNRS URA 1455, Marseille.; OBJECTIVE: To study the structure function relationship of different Tat domains in the mechanism of HIV-1 Tat transactivation. METHODS: Full-length Tat protein Tat 1-86, the gene product of the first exon Tat 1-72 and a panel of shorter peptides mimicking different regions of the primary structure of Tat protein were
Effect of human interferon-induced dsRNA-dependent protein kinase on HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0051). Unique Identifier : AIDSLINE ICA10/94369275; Nagai K; Wainberg MA; Geleziunas R; Sonenberg N; Koromillas AE; McGill AIDS Centre, McGill University, Montreal, Qc, Canada.; The interferon (IFN)-induced dsRNA-dependent protein kinase (PKR) is a serine-threonine kinase that displays two distinct kinase activities: i) activation by autophosphorylation, and ii) phosphorylation of the alpha-subunit of eukaryotic initiation factor, elF-2, which causes inhibition of protein synthesis. PKR activ
In vivo interaction of HIV-1 Rev with an extended Stem I duplex structure of Rev response element RNA.: Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0049). Unique Identifier : AIDSLINE ICA10/94369276; Kimura T; Department of Microbiology, Kansai Medical University, Osaka,; Japan.; OBJECTIVE: Experiments were designed to study whether the packaging reaction of HIV-1 Rev with Rev response element (RRE) RNA reported in vitro mediates Rev function in vivo. METHODS: RRE activity was examined using HIV-1-derived reporter plasmids, which contained the bacterial chloramphenicol acetyltransferase (CAT)
Proviral DNA in virions from primary isolates.: Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0052). Unique Identifier : AIDSLINE ICA10/94369277; Asjo B; Sommerfelt M; National Virus Center, University of Bergen, Norway.; OBJECTIVE: To determine whether proviral DNA is present in HIV virions of primary isolates grown in primary cells. MATERIALS AND METHODS: Patient isolates with slow/low and rapid/high type characteristics were used to infect PHA blasts. Virions were collected by ultracentrifugation and subjected to three different tre
Retroviral integration into highly repetitive Alu and HERV-K elements in 13.1 KB human CD4 locus.: Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0050). Unique Identifier : AIDSLINE ICA10/94369278; Blinov VM; Zverev VV; Nedospasov SA; Institute for Viral Preparations, Moscow, Russia.; Sequence of the human genomic fragment encompassing 13.1 kilobases from the human CD4 locus have been determined. This fragment included three exons and two untrons of CD4 gene. The genomic sequences were analyzed for the presence of repetitive elements and their classification is described. Within the sequenced fragm
Assembly of human immunodeficiency virus matrix protein.: Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0056). Unique Identifier : AIDSLINE ICA10/94369279; Morikawa Y; Kishi T; Nermut M; Hockley D; Jones I; Kitasato Institute, Tokyo, Japan.; OBJECTIVE: To investigate the oligomeric state of p17 matrix protein following expression in E. coli and recombinant baculoviruses. To identify the residues involved in oligomerisation and gag assembly. METHODS: p17 protein was expressed in E. coli and its oligomeric state was examined in vitro. Site-directed mutagene
Internally initiated gag and gag-pol precursors of HIV-1.: Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0053). Unique Identifier : AIDSLINE ICA10/94369280; Kojima A; Tachibana Y; Hoshikawa N; Kurata T; Department of Pathology, National Institute of Health, Tokyo,; Japan.; OBJECTIVE: Inspection of the gag ORF reveales a second methionin (M2) codon which is 10 resides downstream of the p17-p24 junction. Our object is to examine the de novo synthesis of internally initiated gag or gag-pol precursors. METHODS: Mutant gag or gag-pol ORFs either lacking the M2 codon, replaced by a leucine or
The expression of gag-pol and gag-pol-env sequences of HIV-1 by recombinant vaccinia viruses.: Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0054). Unique Identifier : AIDSLINE ICA10/94369281; Grigoriev VB; Gibadulin RA; Liendeman LF; Sazykin AY; D.I. Ivanovsky Institute of Virology, Rus. Acad. Med. Sci.; Moscow.; OBJECTIVE: To study assembly of virus like particle (VLP) in process of expression of gag-pol and gag-pol-env sequences we constructed recombinant vaccinia virus (VV) strains (RVV). METHODS: RVVs were constructed by insertion of the gag-pol and gag-pol-env sequences into VV and termed as RV-1 and RV-2. The expression
The role of the gag protein myristoylation in HIV-1 production.: Int Conf AIDS. 1994 Aug 7-12;10(1):109 (abstract no. PA0055). Unique Identifier : AIDSLINE ICA10/94369282; Furuishi K; Oh-ishi H; Misumi S; Matsuoka H; Ikeda S; Hantani Y; Ashizawa H; Shoji S; Dept. of Biochem., Fac. of Pharm. Sci., Kumamoto Univ., Japan.; OBJECTIVE: The purpose of the studies is to clearly determine the protein myristoylation of the gag protein in HIV-1 infected cell lines, and to resolve the role of the gag protein myristoylation in virion particle formation. METHODS: HIV-1 producing cell lines (CEM/LAV-1, U937/IIIB, U937/RF, H9/MN, Molt-4/IIIB, U1, A
A novel in vitro HIV property in pediatric isolates.: Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 019A). Unique Identifier : AIDSLINE ICA10/94369283; St. Clair M; Vavro CL; Pennington KN; Valentine M; McKinney RE; Burroughs Wellcome Co., RTP NC 27709.; A novel in vitro virus property has been identified in virus isolated from 5 of 28 pediatric patients with HIV disease. This new property differs from the previously defined Syncytia Inducing(SI) and Non-Syncytia Inducing (NSI) phenotypes in that the virus is able to replicate in MT-2 cells without the cytopathogenic
Functional analysis of HIV-1 Gag proteins.: Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 016A). Unique Identifier : AIDSLINE ICA10/94369284; Kawamura M; Kitamura T; Ishimoto A; Adachi A; Institute for Virus Research, Kyoto University, Japan.; OBJECTIVE: To study functions of HIV-1 Gag proteins, various in-frame gag gene mutants were constructed and their replication properties were examined. METHODS: Growth kinetics of mutant viruses in several cell lines were determined by RT assays. Defective steps of the mutants in the viral life cycle were monitored by
HIV infection of adherent cells and expression of membrane molecules.: Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 017A). Unique Identifier : AIDSLINE ICA10/94369285; Dolei A; Serra C; Tilocca F; Ceraulo F; Conaldi PG; Modesti A; Ameglio F; Toniolo A; Inst. of Microbiology & Virology, Univ. of Sassari, Italy.; OBJECTIVE: Human cells of mesenchymal and epithelial origin were analyzed with respect to plasmamembrane molecules and sensitivity to HIV adsorption and replication. METHODS: By the use of a panel of monoclonal antibodies, we evaluated the expression and modulation by HIV infection, of molecules involved in HIV adsorp
Selective susceptibility of human epidermal Langerhans cells to HIV-1 infection in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 018A). Unique Identifier : AIDSLINE ICA10/94369286; Soto-Ramirez LE; Renjifo B; Wang W; Marlink R; Lee TH; Essex M; Department of Cancer Biology, Harvard School of Public Health,; Boston, Massachusetts 02115.; OBJECTIVE: A selective mechanism is probably involved in HIV-1 transmission upon primary infection. As Langerhans cells (LC) can be the primary contact of the virus in heterosexual transmission, the susceptibility of these cells to three HIV-1 infectious molecular clones with defined tropism was investigated. METHODS:
HIV-1 gp160 processing in furin-defective LoVo cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0057). Unique Identifier : AIDSLINE ICA10/94369287; Nagai Y; Ohnishi Y; Shioda T; Institute of Medical Science, University of Tokyo, Japan.; Furin, a subtilisin-like mammalian endopprotease, is believed to be responsible for processing various proprotein precursors of cellular and viral origin including the gp160 of HIV-1, which share the consensus processing site motif, Arg-X-Lys/Arg-Arg, for protease recognition. To confirm and extend the concept that th
Maturation of the HIV gp160 by the Kex2p endoprotease.: Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0058). Unique Identifier : AIDSLINE ICA10/94369288; Moulard M; Kieny MP; Achsterrer T; Montagnier L; Bahraoui E; Universitat Marburg, Germany.; OBJECTIVE: To study the in vitro processing of HIV-1 envelope protein precursor, Kex2p endoprotease from Saccaromyces cerevisiae was used as a model. METHODS: Maturation was examined by using HIV-1 recombinant gp160 or synthetic peptides mimicking the potential cleavage sites of HIV-1, HIV-2 and SIV. The effect of cal
The cytoskeletal proteins cleaving activity of the recombinant HIV-1 protease.: Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0059). Unique Identifier : AIDSLINE ICA10/94369289; Azuma R; Furuishi K; Saito A; Shinagawa H; Ikeda S; Shoji S; Dept. of Biochem., Fac. of Pharm. Sci., Kumamoto Univ., Japan.; OBJECTIVE: The purpose of the present work was to isolate a HIV-1 protease from HIV-1 protease expressing E. coli, and to determine its enzymatic activity for synthetic oligopeptides and nonviral protein substrates. METHODS: HIV-1 protease expressing E. coli was lysed, extracted, and partially purified by DEAE-Cellulo
Transportation system of the viral proteins in the HIV-infected cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):110 (abstract no. PA0060). Unique Identifier : AIDSLINE ICA10/94369290; Takahashi I; Takama M; Ushijima K; Ozel M; Gelderblom H; Centr. Lab. EM. Teikyo Univ. Sch. Med. Tokyo, Japan.; OBJECTIVE: Hitherto the morphological studies on the HIV had revealed only the high-electron dense materials just under the plasma membranes at budding sites. To distinguish hidden features of the viral proteins, combined-technique of immuno electron microscope (IEM) and detergent treatment was applied to the HIV-infe
A computer simulator generating nucleotide sequence variation of the HIV-1 genome.: Int Conf AIDS. 1994 Aug 7-12;10(1):111 (abstract no. PA0063). Unique Identifier : AIDSLINE ICA10/94369291; Doi H; Fujitsu Labs. Ltd., Chiba, Japan.; OBJECTIVE: To know the range of nucleotide sequence variation of the HIV-1 genome, in particular, of the variable regions. It has been assumed that in most cases HIV-1 infection is initiated by a single infectious virion. However, no one knows the range of variation within a patient after infection. I therefore made a
Characterisation of HIV-1 virus isolates involved in mother to child transmission.: Int Conf AIDS. 1994 Aug 7-12;10(1):111 (abstract no. PA0062). Unique Identifier : AIDSLINE ICA10/94369292; Narwa R; Roques P; Boussin F; Marce D; Dormont D; CRSSA/CEA, Fontenay-aux-Roses, France.; OBJECTIVE: We investigated the selection of particular subspecies of mother s human immunodeficiency virus type 1 (HIV-1) isolates during in utero transmission to the infant. METHODS: We characterized HIV-1 isolates involved in mother to child transmission by analyzing cell tropism and V3, V4, V5 env gene sequences. I
Different mechanisms for persistent infection of HIV-1 in MT-4 and MOLT-4 cell lines.: Int Conf AIDS. 1994 Aug 7-12;10(1):111 (abstract no. PA0064). Unique Identifier : AIDSLINE ICA10/94369293; Fujinaga K; Nakaya T; Kameoka M; Kishi M; Ikuta K; Section of Serology, Hokkaido University, Sapporo, Japan.; OBJECTIVE: We previously showed that persistent infection was generated in MT-4 cells by infection with wild-type HIV-1 only after the serial passage. On the other hand, MOLT-4 could survive the infection with wild-type HIV-1. Here, we examined different mechanisms for persistent infection in MT-4 and MOLT-4 cell line
Mutations of basic amino acids of NCp7 of HIV-1 affect RNA binding in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):111 (abstract no. PA0061). Unique Identifier : AIDSLINE ICA10/94369294; Schmalzbauer E; Dannull J; Guehmann S; Moelling K; Max-Planck-Institute for Molecular Genetics, Berlin, FRG.; OBJECTIVES: The nucleocapsid (NC) protein of human immundeficiency virus type 1 (HIV-1) is necessary during virion assambly for core formation and packaging of the viral genomic RNA dimer. The NC protein of HIV-1 contains two clusters of basic aminoacids consisting of five and four aminoacid residues respectively, fla
AIDS pathogenesis: drug abuse, cytokines, and viral strain.: Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0065). Unique Identifier : AIDSLINE ICA10/94369295; Xin K; Shapshak P; Nagano I; Stewart R; Dept. of Psychiatry, Univ. of Miami Medical School, FL 33136.; OBJECTIVE. We are investigating the pathogenesis of AIDS due to HIV-1 infection, drug abuse, and cytokines. METHODS. We utilize culture and molecular techniques (in situ hybridization and immuno-histochemistry to detect HIV and identify cell types in the central and peripheral nervous systems) and PCR, cloning, and DN
Darwinian evolution operates on the V3 region of HIV within a single host.: Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0066). Unique Identifier : AIDSLINE ICA10/94369296; Yamaguchi Y; Gojobori T; National Institute of Genetics, Shizuoka, Japan.; OBJECTIVE: The purpose of this study is to elucidate the mechanisms of molecular evolution of the third variable envelope (V3) region for HIV within a single host. In particular, we intend to examine the patterns of nucleotide substitutions in this region to see whether positive selection is operating on the V3 region
Diversity (macrophage-tropism) in the V3 loop region of HIV-1 gp120 from saliva.: Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0068). Unique Identifier : AIDSLINE ICA10/94369297; Kakizawa J; Ando S; Morishita Y; Nishimura S; Oka S; Mori S; Muller W; Ushijima H; Department of Microbiology, Institute of Public Health, Tokyo,; Japan.; OBJECTIVE: To analyze the V3 region of HIV-1 gp120 from saliva and to consider the diversity of HIV-1 from the other organs. MATERIALS AND METHODS: The saliva and blood samples from HIV positive patients were collected and HIV DNAs and RNAs were extracted by glass powder. TA cloning substances of PCR or RT-PCR product
Patterns of amino acid substitutions in the third variable envelope region for HIV within a host.: Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0069). Unique Identifier : AIDSLINE ICA10/94369298; Gojobori T; Yamaguchi Y; National Institute of Genetics, Shizuoka, Japan.; OBJECTIVE: The purpose of this study is to examine the patterns of amino acid changes in the third variable envelope (V3) region of HIV by phylogenetic methods. The substitution patterns obtained would be useful to develop effective vaccines. METHODS: We constructed phylogenetic trees by use of nucleotide sequences of
An analysis of the V3 gene of HIV-1 isolated in Uganda.: Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0071). Unique Identifier : AIDSLINE ICA10/94369299; Mitarai S; Byarugaba W; Oishi K; Nagatake T; Matsumoto K; Fukasawa M; Department of Internal Medicine, Nagasaki University, Japan.; OBJECTIVE: To isolate the most reproductive HIV-1 strain from Ugandan HIV-1 infected patients and determine its genotype with the V3 loop sequence. METHODS: Peripheral blood samples were collected from the HIV-1 seropositive patients at Mulago hospital in 1991. The patients were one male and 5 female, and the mean age
HIV-1 V3-loop antibodies and it's association with clinical status in Nairobi.: Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0070). Unique Identifier : AIDSLINE ICA10/94369300; Songok M; Tukei P; Mulaa FJ; Kenya Medical Research Institute, Nairobi.; In our bid to determine the HIV-1 variants in transmission in Nairobi and their association with clinical status and infectivity, we screened by ELISA two hundred and seven (207) confirmed HIV positive patients visiting our HIV/AIDS clinic in Nairobi between June 1993 and January 1994. We used a panel of HIV-1 V3 loop
Increasing persistent infection of serial passages of HIV-1 is correlated with mutations at vif and vpr regions.: Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0073). Unique Identifier : AIDSLINE ICA10/94369301; Nakaya T; Fujinaga K; Nishino Y; Kishi M; Ikuta K; Section of Serology, Hokkaido University, Sapporo, Japan.; OBJECTIVE: We have shown that MT-4 cells survived the infection with vif-, vpr-, or vpu-mutant HIV-1, but not with wild-type or nef-mutant HIV-1. However, increasing persistent infection in the cells were observed by serial passage of wild-type HIV-1, but not by serial passage of nef-mutant HIV-1 even after 50 passage
Unusual A/G ratio in HIV-1 gp120 V3 loop proviral sequences obtained in vivo.: Int Conf AIDS. 1994 Aug 7-12;10(1):113 (abstract no. PA0072). Unique Identifier : AIDSLINE ICA10/94369302; Gomez Carrillo M; Rabinovich R; Marquina S; Galvan V; Libonatti O; Natl. Centre of Ref. for AIDS, Buenos Aires, Argentina.; OBJECTIVE: To analyze A/G ratio values of proviral V3 loop HIV-1 sequences and correlate them with some phenotypic features of the corresponding variants. METHODS: After sequencing using the Sanger method, A/G ratio values were calculated for 28 in vivo, 2 isolate Argentine sequences and 21 published worldwide isolate
Analysis of HIV-1 nef gene sequences directly isolated from infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0076). Unique Identifier : AIDSLINE ICA10/94369303; Kim SH; Kang MR; Lee MH; Huh JE; Shin Y; Kim DG; Kim TG; Cho MH; Kim S; Seoul National University, Korea.; The nef gene is located near the 3 end of the HIV genome and overlaps the 3 long terminal repeat. Although the term nef is the acronym of negative factor, the proposed negative role of the nef gene has been challenged by us and others. It was recently reported that in rhesus monkeys, the nef gene of SIV is required
Biological properties of HIV-1 strains occurring in the Ukraine.: Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0077). Unique Identifier : AIDSLINE ICA10/94369304; Antonenko S; Barbasheva E; Green E; Lujlchujk M; Persidsky Y; Frolov A; Scientific Research Institute of Epidemiology and Infectious; Diseases, Kiev, Ukraine.; OBJECTIVE: To study biological properties of HIV isolated from a viruses carrier in the Ukraine . METHODS: Fluorescent antibody technique, ELISA of HIV-1 p24 antigen of the culture supernatant, RT-activity, electron microscopy, FCR, immunohistochemical characterization of native Ne
Conserved domains of the HIV-1 vif gene in vivo.: Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0075). Unique Identifier : AIDSLINE ICA10/94369305; Wieland U; Seelhoff A; Salzberger B; Schwander S; Dietrich M; Eggers HJ; Kuehn JE; Institut f. Virologie, Universitaet Koeln, Germany.; OBJECTIVE: Determination of the genetic variability of the HIV-1 vif gene and identification of conserved and possibly functionally important regions of the complete vif gene of European and African HIV-1-infected patients. METHODS: PCR products of peripheral blood mononuclear cells extracts of 85 HIV-1 positive patie
Genetic heterogeneity in vif and vpr regions of HIV-1 in PBMC from asymptomatic carriers.: Int Conf AIDS. 1994 Aug 7-12;10(1):114 (abstract no. PA0074). Unique Identifier : AIDSLINE ICA10/94369306; Kishi M; Zheng YH; Tokunaga K; Bahmani MK; Tateno M; Tateno Y; Oka S; Kurata T; Kakinuma M; Ikuta K; Inst. Immunol. Sci., Hokkaido Univ., Sapporo, Japan.; OBJECTIVE: MT-4 cells survived the infection with vif, vpr, or vpu mutant HIV-1 after transient cytopathicity, suggesting the occurrence of additional mutation(s) during viral replication. Here, we examined naturally occurring additional mutations in accessory genes essential for lead to persistent infection. In addit
AIDS in HIV1/HIV2 seronegative patient in Yaounde-Cameroon.: Int Conf AIDS. 1994 Aug 7-12;10(1):115 (abstract no. PA0079). Unique Identifier : AIDSLINE ICA10/94369307; Zekeng L; Afane ZE; Gurtler L; Ondoa P; Knapp S; Eberle J; Kaptue L; Laboratoire d'hematologie, CHU Younde Cameroun.; OBJECTIVE: To describe a case of HIV infection in a Cameroonian patient antibody negative using commercial available EIA and rapidassays. METHODS: Miss A., 24 years old was hospitalized for poor general state, productive cough, persistant diarrhoea and oral candidiasis. A chest Xray and sputum examination confirmed a
Possible role of dUTPase analog in compositional divergency in evolution of retriviruses.: Int Conf AIDS. 1994 Aug 7-12;10(1):115 (abstract no. PA0081). Unique Identifier : AIDSLINE ICA10/94369308; Blinov VM; Resenchuk SM; Chirikova GB; Denisov SI; Zverev VV; Institute of Molecular Biology, Koltsovo, Novosibirsk, Russia.; HIV, unlike other lentiviruses, has high evolutionary rate in the accumulation of mutations. Earlier we have revealed a new enzymatic activity (dUTPase EC 3.6.4.23) in the subfamily of lentiviruses, which includes VISNA, EIAV, FIV, and CAEV. However, in the subfamily of lentiviruses including HIV1, HIV2, SIV, and HTLV
Probably reasons of hypervariability by AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):115 (abstract no. PA0080). Unique Identifier : AIDSLINE ICA10/94369309; Deichman AM; Cancer Research Centre, Moscow, Russia.; OBJECTIVE: Any information (especially new) about AIDS is important. There aren t whole universal picture (even scetch) about nature, reasons, mechanisms for hypervariability formation by AIDS in biopshere. METHODS: theoretical reseaches show the main mechanisms of hypothetical concept may be two: IERT-individual epit
Serodiagnostic profiles of HIV-1 from Nigeria.: Int Conf AIDS. 1994 Aug 7-12;10(1):115 (abstract no. PA0078). Unique Identifier : AIDSLINE ICA10/94369310; Offor E; Mclane MF; Trujillo R; Kanki P; Essex M; Dept. Cancer Biology, Harvard School of Public Health, Boston, MA; 02115.; OBJECTIVE: To compare the serum antibody profiles of a Nigerian HIV-1 viral isolate with those of an African (Congo) and a North American isolate (USA). METHOD: Three HIV-1 antigens, USA type IIIB, a Congolese isolate and a Nigerian isolate (N-4), were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrop
Evidence for the presence of North American type HIV-I in Hyderabad, India.: Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0082). Unique Identifier : AIDSLINE ICA10/94369311; Rao R; Baskar P; Ray S; Quinn T; Hildreth JE; Bollinger R; Apollo Hospital, Hyderabad, India.; OBJECTIVE: To analyse the strains from Southern part of India . METHODS: 1. HIV-1 envelope DNA was amplified by nested polymerase Chain Reaction from the peripheral blood mononuclear cells of HIV-1 infected individuals. 2. Amplified DNA was sequenced using automated flourescein based
Full sequence of gp120 of an HIV-1 variant (GWGR) highly prevalent in Brazil.: Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0085). Unique Identifier : AIDSLINE ICA10/94369312; Costa SM; Shindo N; Vincente AN; Pinto ME; Schechter M; Tanuri A; Dept. of Genetics, UFRJ.; INTRODUCTION: Data available from Brazil , a country selected by WHO for future vaccine trials, indicate that most circulating strains belong to clade B. On the other hand, it has also been shown that in Brazil the motif GWGR at the tip of the V3 loop is almost as common as GPGR. Th
The V3 region of HIV-1 isolates prevalent in Taiwan contains a unique signature pattern in addition to the Thailand E subtype sequence.: Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0083). Unique Identifier : AIDSLINE ICA10/94369313; Chen YM; Sheu TT; Lee CM; Jang YJ; AIDS Research Center, National Yang-Ming Medical College, Taipei,; Taiwan, R.O.C.; OBJECTIVE: To study the genetic variation of the V3 region in the env gene from HIV-1-infected persons in Taiwan . To conduct phylogenetic analyses of the Taiwanese HIV-1 isolates. METHODS: HIV-1 proviral DNA was PCR amplified using
V3 region sequences from HIV-1 isolates from India.: Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0084). Unique Identifier : AIDSLINE ICA10/94369314; Tripathy S; Renjifo B; McLane MF; Tripathy SP; Essex M; Dept. of Cancer Biology, Harvard School of Public Health, Boston,; Ma 02115.; AIM: To determine the V3 region sequences from HIV-1 isolates from heterosexually infected patients from India . METHODOLOGY: PBL samples were cocultured with normal PBLs and the DNA was extracted for PCR. The 1.5 kb gp120 fragment was cloned and the V3 region sequenced. RESULTS: All
Complement activation in HIV infected patients with cryoglobulinemia.: Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0088). Unique Identifier : AIDSLINE ICA10/94369315; Schmit JL; Redeker S; Fuentes V; Prin L; Infectious Disease Unit, University Hospital Amiens, France.; The aim of the study was to investigate complement activation in HIV infected patients with cryoglobulinemia. In a previous study we have noticed that HIV infected patients with cryoglobulinemia had low activation fragments of the classical (C4d) and the common (C3d) pathway. The presence of cryoproteins in these pati
Modeling HIV pathogenesis using artificial life concepts and methods.: Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0087). Unique Identifier : AIDSLINE ICA10/94369316; Baehr M; Mind Designs, New York, NY.; OBJECTIVE: To develop a computer simulation of HIV pathogenesis using concepts and methods from artificial life research in order to enhance understanding of the complexity and variability of immune system/HIV interactions. METHODS: A database including 50 cell types and 10 HIV strain types with 60 pseudogenes each. P
Phenotypic heterogeneity between peripheral blood (PB) and lymph node (LN) HIV isolates.: Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0086). Unique Identifier : AIDSLINE ICA10/94369317; Tamalet C; Lafeuillade A; Vignoli C; Rousseau S; DeMicco P; Department of Virology, TimoneHospital, Marseille, France.; OBJECTIVE: Previous results obtained by PCR or coculture techniques have shown higher viral burden in lymph node mononuclear cells (LNMCs) than in peripheral blood mononuclear cells(PBMCs). The aim of this study was to comparate the phenotype of HIV simultaneously isolated from PB and LN in HIV infected patients (pts)
Relationship between infectious HIV-1 viral load, CD4 count and clinical status.: Int Conf AIDS. 1994 Aug 7-12;10(1):117 (abstract no. PA0089). Unique Identifier : AIDSLINE ICA10/94369318; Margolick J; Farzadegan H; Hoover D; Rubb S; Scott E; Saah A; Johns Hopkins University, Baltimore, MD 21205.; OBJECTIVE: To define the interaction between infectious viral load and the host immune system. METHOD: 246 HIV+ homosexual men in the Johns Hopkins Center of the Multicenter AIDS Cohort Study (MACS) were studied. The 1987 clinical AIDS definition was used. Infectious units of HIV-1 per one million PBMC (IUPM) was dete
Amplification of viral replication relevantly with reverse differentiation in HIV-1 infected cells with macrophage phenotype.: Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0091). Unique Identifier : AIDSLINE ICA10/94369319; Okada Y; Kameoka M; Kimura T; Kishi M; Ikuta K; Institute of Immunological Science, Hokkaido University, Sapporo,; Japan.; OBJECTIVE: The cells of monocyte-macrophage lineage was important as HIV-1 reservoir, especially for understanding the persistent mechanism in asymptomatic carriers. Here, we examined the HIV-1 life cycle after the infection of differentially differentiated cells. METHODS: U937 cells were differentially differentiated
Difference in surface structure between mature and defective HIV virus particles.: Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0093). Unique Identifier : AIDSLINE ICA10/94369320; Morita C; Ashina T; Sukenaga A; Ikuta K; Goto T; Nakai M; Department of Microbiology, Osaka Medical College, Japan.; OBJECTIVE: Surface projections(knobs) are essential for binding HIV-1 to target cells, and spontaneous loss of them reduces infectivity of HIV-1. Our objective is to clarify the structure difference of surface projections between mature and defective particles. METHOD: Persistently HIV-1 infected Molt-4 and infected M
Increased degradation of newly synthetized proteins in T-lymphocytes from HIV+ asymptomatic individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0092). Unique Identifier : AIDSLINE ICA10/94369321; Piedimonte G; Silvotti L; Montroni M; Silvestri G; Guetard D; Montagnier L; University of Parme, Italy.; OBJECTIVE: Among early functional T-cell defects occuring after HIV infection, the reduction of IL-2 production and the reduced expression of surface molecules strongly suggest abnormalities at some stage of the cellular protein synthetic process. The aim of this work 2
Variation in susceptibility to HIV-1 & 2 of PBMCs from healthy Bangladeshi, and correlation with HLA type.: Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0090). Unique Identifier : AIDSLINE ICA10/94369322; al-Jabri AA; Taylor M; McCloskey D; Sefton A; Race E; Navarrete C; Oxford JS; Dept. Med. Microbiology, London Hospital Medical College,; University of London, U.K.; We describe an in vitro study, within a specific ethnic group, where the association between the HLA type and susceptibility/resistance to HIV infection is investigated. PBMCs from healthy Bangladeshi male subjects were infected with 4 different isolates of HIV-1 & 2 (an HIV-1 clinical isolate, HIV-1 MN, HIV-2 CBL2
Chromosome studies of HIV-infected and uninfected cell lines and blood lymphocytes.: Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0097). Unique Identifier : AIDSLINE ICA10/94369323; Manolov G; Argirova R; Manolova Y; Central AIDS Lab., Sofia, Bulgaria.; OBJECTIVE: To study in vitro a possible chromosome aberrational role of HIV. METHODS: A chromosome analysis of two cell systems was carried out: 1) peripheral blood lymphocytes (PBL)-uninfected and infected with HIV from H9/HTLV-IIIB of 5 healthy donors and 2/H9/HTLV-IIIB cells and their uninfected variant. RESULTS: Aa
Cytokine production and viral replication in human MDMs treated with RP55778, a TNF alpha synthesis inhibitor, prior to HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0096). Unique Identifier : AIDSLINE ICA10/94369324; Le Naour R; Henin Y; Raoul H; Bousseau A; Mabondzo A; Clayette P; Dormont D; IFRC, CEA, Fontenay aux Roses, France.; OBJECTIVES: To understand the relationships between HIV1 infection and cytokine secretion by using a TNF alpha synthesis inhibitor, RP55778. MATERIAL & METHODS: Human monocytes were isolated from peripheral blood mononuclear cells by counter current elutriation. Seven days after isolation, monocyte-derived macropha
Highly LFA-1-expressing U937 subclones showing lower susceptibility to HIV-1 can lead to continuously stable virus production after long persistent period.: Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0095). Unique Identifier : AIDSLINE ICA10/94369325; Kameoka M; Okada Y; Fujinaga K; Kimura T; Fujii N; Ikuta K; Inst. Immunol. Sci., Hokkaido Univ., Sapporo, Japan.; OBJECTIVE: Heterogeneous HIV-1 life cycles in monocyte-macrophage lineage were examined using U937 subclones for understanding the mechanisms of HIV-1 latent or persistent infection. METHODS: A total of 46 clones (#1-#46) were isolated from U937. The sensitivity to HIV-1 infection was measured by the appearance of HIV
Surface expression of p17-gag protein during HIV-1 reactivation.: Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0094). Unique Identifier : AIDSLINE ICA10/94369326; Yoshimura K; Matsushita S; Matsumi S; Obaru K; Takatsuki K; Second Department of Internal Medicine, Kumamoto University; School of Medicine, Japan.; OBJECTIVE: The purpose of this study was to investigate the surface expression of HIV-1 antigens on latently infected cell lines during reactivation. MATERIALS AND METHODS: CEM/LAV1 and H9/IIIB were used as the chronically infected cell lines. ACH-2 (T-cell line), OM-10.1 (myeloid cell line), and U1 (promonocyte cell
Encounter of unprimed CD4+ T lymphocytes with HIV-1 gp120-expressing cells results in lysis of both target and effector cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):12 (abstract no. 020A). Unique Identifier : AIDSLINE ICA10/94369327; Jassoy C; Heinkelein M; Sopper S; Institute for Virology and Immunobiology, Wurzburg University,; Germany.; OBJECTIVE: To examine the mechanism underlying the lysis of HIV-1 and gp160 expressing cells by non-specific CD4+ T lymphocytes. METHODS: B lymphoblastoid cells infected with recombinant vaccinia viruses expressing HIV-1 gp 160 or control HIV-1 proteins and HIV-1-infected or uninfected T cell lines were used as target
Glucocorticoids rescue CD4+ T cells from activation-induced apoptosis triggered by HIV-1.: Int Conf AIDS. 1994 Aug 7-12;10(1):12 (abstract no. 021A). Unique Identifier : AIDSLINE ICA10/94369328; Lu W; Salerno-Goncalvez R; Yuan J; Andrieu JM; Laboratory of Tumor Immunology, Hopital Laennec, Universite de; Paris V, France.; OBJECTIVE: Having observed an increase of the CD4 count in HIV+ patients treated by prednisolone (Abstr., Andrieu Jean-Marie, et al), we investigated the in vitro effects of glucocorticoids (GCC) on HIV+ T-cell apoptosis. METHODS: PBMC from HIV neg. donors were separated by Ficoll-Hypaque gradient and CD4 and CD8 cell
HIV-1 infection of T cells in vitro is modulated by costimulation via CD28.: Int Conf AIDS. 1994 Aug 7-12;10(1):12 (abstract no. 022A). Unique Identifier : AIDSLINE ICA10/94369329; Haffar OK; Smithgall M; Wong J; Linsley P; Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle,; Washington 98121.; OBJECTIVE: We wanted to study the role of the T cell accessory molecule CD28 in the life cycle of HIV-1. METHODS: Peripheral blood mononuclear cells were isolated from blood of HIV-1 infected and uninfected donors, depleted of CD8+ T cells, and activated with a soluble anti-CD3 mAb alone or in combination with a anti-
Identification of a complement activating site in gp120.: Int Conf AIDS. 1994 Aug 7-12;10(1):12 (abstract no. 023A). Unique Identifier : AIDSLINE ICA10/94369330; Susal C; Kirschfink M; Kropelin M; Daniel V; Opelz G; Institute of Immunology, University of Heidelberg, Germany.; OBJECTIVE: We showed recently that rgp120 of HIV-1, either attached to microtiter plates or bound to normal CD4+ cells, activates the human complement system in the absence of anti-gp120 antibodies. This may represent a mechanism for the elimination of uninfected CD4+ cells by the reticuloendothelial system and play a
Acquired specific cytotoxic activity in CD8+ HIV-1IIIB carrier cell clones derived from CD4+CD8+ parental human T cell leukemia cell lines.: Int Conf AIDS. 1994 Aug 7-12;10(1):120 (abstract no. PA0100). Unique Identifier : AIDSLINE ICA10/94369331; Minowada J; Otani T; Kamiya T; Fujisaki Cell Center, Okayama, Japan.; OBJECTIVE: To determine the functional activity of CD8 positive HIV-1 carrier cell clones. METHOD: A 4-hr two-color flow cytometric cytotoxicity assay was used. The effector/target cell ratio analyzed was in the 0.2-3.0/1 range. RESULTS, DISCUSSION AND CONCLUSION: Seven HIV-1IIIB carrier CD8+ MOLT-4 clones from T-cell
Analysis of the cellular transduction pathways regulating HIV genome transcription as potential targets for antiviral therapy. European Communities Concerted Action.: Int Conf AIDS. 1994 Aug 7-12;10(1):120 (abstract no. PA0101). Unique Identifier : AIDSLINE ICA10/94369332; Virelizier JL; Arenzana F; Alcami J; Hay RT; Moscat J; Institut Pasteur, Paris, France.; Presently available anti-HIV chemotherapies are mostly aimed at blocking HIV reverse transcriptase (RT) activity. However, as soon as the HIV genome is integrated into host cell DNA, such mechanism of action cannot suppress virus replication. Blocking transcription of integrated HIV provirus would be expected to syner
Establishment of HIV-1-producing T cells from peripheral lymphocytes: participation of human complement factor B.: Int Conf AIDS. 1994 Aug 7-12;10(1):120 (abstract no. PA0098). Unique Identifier : AIDSLINE ICA10/94369333; Nozaki-Renard J; Hirabuki N; Mizuno F; Iino T; Tada T; Dept. Microbiol. Tokyo Medical College.; OBJECTIVE: Research on the death and on the survival of HIV-1-infected CD4+ T cells in human serum as a milieu supporting viral target cells. STUDY SUBJECTS AND METHODS: 14 HIV-1-seroconverted individuals aged 8 to 65 (2 AC, 9 ARC and 3 AIDS cases). 4 male laboratory workers, as healthy peripheral mononuclear cells (P
Transformation of human diploid fibroblasts by a transmissible agent obtained from an HIV-infected patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):120 (abstract no. PA0099). Unique Identifier : AIDSLINE ICA10/94369334; Dolei A; Serra C; Gentile M; Falcone V; Conaldi PG; Casalone R; Toniolo A; Institute of Microbiology, Univ. of Sassari, Italy.; OBJECTIVE: To study the effects of clinical HIV-1 isolates on human fibroblastoid cells. Exposure to one of the isolates was followed by immortalization of MRC-5 diploid fibroblasts. The cell-free supernatant of these cells was able to immortalize new batches of MRC-5 fibroblasts. Uninfected and HIV-1 infected MRC-5 c
Multiple input of infectious HIV-1 is essential to single cell killing.: Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0104). Unique Identifier : AIDSLINE ICA10/94369335; Kuroda MJ; el-Farrash MA; Kannagi M; Harada S; Kumamoto University School of Medicine, Japan.; OBJECTIVE: To study the mechanism of single cell killing by HIV-1 in CD4+ T cell line. METHODS: MT-4 cells were infected with different moi (1, 10 and 30) of HIV-1 in the presence or absence of neutralizing monoclonal antibody 0.5 beta or dextran sulfate added soon after adsorption. Cell growth and viability of the in
Polyunsaturated fatty acids reduce gp120-induced syncytia formation.: Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0102). Unique Identifier : AIDSLINE ICA10/94369336; Aloia RC; Tian HR; Mlekusch W; VanGent CM; Jensen FC; Anesthesia Service, J.L. Pettis Veterans Hospital, Loma Linda, CA; 92357.; OBJECTIVE: We have previously demonstrated that the anti-Leu3A mAb- inhibitable fusion between 8E5 cells, carrying the HIV genome (minus the pol gene) and bearing gp120, with Molt3 cells expressing CD4+ receptors, was significantly reduced after treating the cells with the long chain, polyunsaturated fatty acid, docos
Properties of the enzyme dipeptidyl peptidase IV (DPP IV/CD26) from a chemical viewpoint.: Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0103). Unique Identifier : AIDSLINE ICA10/94369337; Barth A; Neubert K; Born I; Heins J; Faust J; Rahfeld J; Brandt W; Hovanessian AG; A.G.M.V. Institute of Biochemistry, University of Halle, Germany.; DPP IV/CD26 hydrolyzes dipeptide units from the N-termini of peptides and proteins, preferable at the C-terminal side of proline residues. A substrate is recognized when the N-terminal amino group is protonated (positive changed). The best substrates are when in the P1-position is a proline or a alanine residue, where
Soluble CD95 (FAS delta TM) inhibits HIV-related apoptosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):121 (abstract no. PA0105). Unique Identifier : AIDSLINE ICA10/94369338; Krowka JF; Sheppard HW; Ascher MS; Fitzpatrick P; Kiefer MC; Pavloff N; Barr PJ; VRDL, CA Dept. of Health Services, Berkeley 94704.; OBJECTIVE: To analyze the role of the apoptosis/activation antigen FAS (CD95) in depletion of lymphocytes from HIV-infected (HIV+) individuals. METHODS: Peripheral blood lymphocytes (PBL) from HIV+ and HIV-seronegative (HIV) participants of the San Francisco Men s Health Study were stained with monoclonal antibodies a
Induction of demyelination and neuronal death by HIV-1 gp120 depends on neural development.: Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0107). Unique Identifier : AIDSLINE ICA10/94369339; Kimura-Kuroda J; Nagashima K; Yasui K; Tokyo Metro. Inst. for Neurosci., Japan.; OBJECTIVE: In HIV encephalopathy, diffuse demyelination and neuronal loss have been observed frequently, while the pathogenesis remains obscure. Although the neurotoxicity of gp120 as a causative factor has been reported, it is still not recognized. To clarify the neurotoxicity of gp120, its effects on neural cells we
Monomeric HIV-gp120 induces both inhibition of cell proliferation and apoptosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0106). Unique Identifier : AIDSLINE ICA10/94369340; Tuosto L; Lorenzetti S; Cundari E; Moretti S; Lombardi G; Piccolella E; Dept. Cell. and Develop. Biology, Univ. of Rome, Italy.; Infection with human immunodeficiency virus (HIV) results in a progressive impairment of immune responses, partly due to the defect induced in T cells following the interaction of the HIV-envelope glycoprotein (gp120) and/or anti-CD4 antibodies with CD4 molecules. These effects have been reported as inhibition of mito
Regulation of the MnSOD gene in HIV infected macrophages.: Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0109). Unique Identifier : AIDSLINE ICA10/94369341; Raoul H; Le Naour R; Mabondzo A; Dormont D; SSA/DSV, CEA, Fontenay aux Roses, France.; OBJECTIVE: Because an imbalance between oxydant and anti-oxydant has been demonstrated in HIV-1 seropositive individuals, and because TNF alpha seems to regulate the expression of enzymes of the oxidative burst, like the MnSOD, we tried to determined: 1) the effect of HIV-1 infection on the MnSOD and TNF alpha genes e
Salivary anti-HIV activity.: Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0108). Unique Identifier : AIDSLINE ICA10/94369342; Nagshunmugam T; Friedman H; Davis C; Abrams W; Malamud D; U. Penn. Dent. Med. Phila. 19104.; OBJECTIVE: Human saliva decreases HIV-1 infectivity. Some of this inhibition requires filtration and is associated with viral aggregation. The present study describes the nature of a second type of salivary anti-HIV activity. METHODS: Submandibular saliva was collected from 15 seronegative individuals. After dialysis
Association between changes of HIV-1 quasispecies in serum and disease progression.: Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0112). Unique Identifier : AIDSLINE ICA10/94369343; Ida S; Oka S; Hirabayashi Y; Shioda T; Nagai Y; Kimura S; Shimada K; Institute of Medical Science, University of Tokyo, Japan.; OBJECTIVE: In order to address the virulent variants which correlate with clinical deterioration, we analyzed the genetic changes of HIV-1 in sera obtained from three patients whose CD4 counts had declined rapidly. MATERIALS AND METHODS: All patients are Japanese hemophiliacs who were infected with HIV-1 at least befo
Coselection in AIDS pathogenesis.: Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0111). Unique Identifier : AIDSLINE ICA10/94369344; Hoffmann GW; Immune Network Research Ltd., Vancouver, Canada.; A model of AIDS pathogenesis in the context of an idiotypic network model is presented. Many phenomena related to AIDS have been described. It is important that we formulate and develop a conceptual model that can account for as wide a variety of the phenomena as possible. Phenomena to be explained include the prevale
CD4 cross-linking induces aberrant cytokine secretion. Fas antigen expression and T-cell apoptosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0110). Unique Identifier : AIDSLINE ICA10/94369345; Oyaizu N; McCloskey TW; Than S; Hu R; Pahwa S; North Shore University Hospital-Cornell University Medical; College, New York.; OBJECTIVE: We have recently shown that in unfractioned peripheral blood mononuclear cells (PBMC), the crosslinking of CD4 (CD4XL) by itself is sufficient to induce T cell apoptosis. The aim of the present study was to examine the mechanism of CD4XL-induced T cell apoptosis. We have focused on Fas antigen (Fas) express
Lipid peroxidation role in formation of immunodeficiency in HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0113). Unique Identifier : AIDSLINE ICA10/94369346; Pastushenkov V; Mitin Y; Karpischenko A; Ivanov G; Military Medical Academy, St. Petersburg, Russia.; OBJECTIVE: To study the influence of lipid peroxidation on lymphocyte function and nuclear chromatin degradation in the course of immunodeficiency progression in HIV-1 infection. METHODS: 37 HIV-1 infection patients (WB and ELISE) persons were investigated. T and B cells subsets were counted by cytofluorimetric assay.
DNA staining changes associated with apoptosis and necrosis in lymphocytes of individuals with HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0115). Unique Identifier : AIDSLINE ICA10/94369347; Echaniz P; de Juan MD; Cuadrado E; Hosp. Ntra. Sra. de Aranzazu, San Sebastian, Spain.; OBJECTIVE: We report here a flow cytometry method dealing with the quantitation of cells which die by apoptosis or necrosis, and its uses in individuals at different stages of HIV infection. METHOD: The method uses low concentrations of two DNA binding dyes tha allow to establish selective regions for live, apoptotic
HIV infection induces thymocyte depletion in the SCID-hu mouse.: Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0117). Unique Identifier : AIDSLINE ICA10/94369348; Kaneshima H; Bonyhadi M; Su L; Connor R; Ho D; McCune JM; SyStemix, Inc., Palo Alto, California 94304.; A variety of mechanisms have been proposed to account for the observed loss of CD4+ cells after HIV infection. However, little is known about the events which occur within infected lymphoid organs wherein most CD4 T cells mature and function. To understand HIV pathogenesis in vivo, we have developed a SCID-hu mouse mo
Primary infection in lymph nodes of macaques inoculated with pathogenic and attenuated SIV.: Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0116). Unique Identifier : AIDSLINE ICA10/94369349; Chakrabarti L; Cumont MC; Baptiste V; Khatissian E; Montagnier L; Hurtrel B; Unite d'Oncologie Virale, Institut Pasteur, Paris, France.; Our purpose was to characterize the very early events that distinguish pathogenic and attenuated SIVmac infections. Using in situ hybridization, we determined the kinetics of viral spread in lymph nodes (LN) obtained sequentially during the primary stage of infection. 4 Rhesus macaques were inoculated intravenously wi
The role of nitric oxide in patients with HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0114). Unique Identifier : AIDSLINE ICA10/94369350; Torre D; Ferrario G; Bonetta G; Zeroli C; Giola M; Fiori GP; Div. Infectious Diseases, Varese, Italy.; OBJECTIVE: Nitric oxide (NO) and its intermediates, nitrite and nitrate, exert microbiostatic and microbicidal effects against protozoa, fungi, bacteria and some viruses, including herpes simplex virus. NO modulates immune responses by mediating the regulation of lymphocyte proliferation and it influences local inflam
Cytofluorimetric and immunoelectronmicroscopic analysis of GM3 expression on lymphocytes from HIV infected patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0120). Unique Identifier : AIDSLINE ICA10/94369351; Sorice M; Pavan A; Garofalo T; Circella A; d'Agostino F; Sansolini T; Pinter E; Lenti L; Torrisi MR; Dip. Med. Sperimentale, Universita La Sapienza, Roma, Italy.; Recently, we demonstrated (Misasi et al. Clin Immunol. Immunopathol., 67: 216, 1993) that lymphocytes from HIV infected patients have an increased concentration of GM3 as compared to lymphocytes from healthy donors or patients suffering from different viral diseases (infectious mononucleosis, acute B or chronic C hepa
Effects on virological parameters of passive immunotherapy in AIDS patients. The PI Collaborative Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0118). Unique Identifier : AIDSLINE ICA10/94369352; Joubert L; Chevret S; Vittecoq D; Lefrere JJ; Hopital St-Antoine, Paris.; Eighty two CDC IV patients were evaluated in a double blind phase II trial and randomized between 2 groups: infusions of plasma rich in anti-p24 ab (HIV+P) or seronegative plasma (HIV-P). Each patient received infusion every 14 days over a one-year period and every 28 days beyond; p24 ag titer was quantified through m
Interleukin 12 enhances host responses of HIV positive donors against Mycobacterium avium in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0121). Unique Identifier : AIDSLINE ICA10/94369353; Newman G; Guarnaccia J; Vance E; Remold H; Kazanjian P; Dept. Rheum/Immunol, Brigham & Women's Hospital/Harvard Medical; School, Boston, MA 02115.; OBJECTIVE: To determine whether IL-12 could enhance growth inhibition of an proliferative responses to M. avium by peripheral blood mononuclear cells (PMBC) from either HIV negative or positive donors. METHODS: PBMCs from 14 HIV+ and 12 HIV- donors were inoculated with M. avium, serovar 4, and cultured for 7 days in t
More apoptosis of T-cell subsets were observed in the more advanced HIV-infected individuals using BM-1/JIMRO mAb and flow cytometric analysis.: Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0119). Unique Identifier : AIDSLINE ICA10/94369354; Ruxrungtham K; Kashiwagi N; Adachi M; Sirivichayakul S; Maruyama I; Phanuphak P; Chulalongkom Hospital, Bangkok, Thailand.; BACKGROUND: Apoptosis is one of the proposed mechanisms of the depletion of CD4+ T-cells in AIDS. BM-1/JIMRO mAb recognizes Le(Y) carbohydrate antigen which is closely correlated with the process of apoptosis. OBJECTIVE: To analyse the expression of an apoptotic marker-BM-1 (Le(Y)) Ag on peripheral blood T-cell subset
Evaluation of cell culture in the diagnosis of cerebral and pulmonary toxoplasmosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0125). Unique Identifier : AIDSLINE ICA10/94369355; Contini C; Magno S; Romani R; Zenobi P; Angelici E; Cultrera R; Delia S; Inst Infect and Resp Dis. University of Ferrara, Italy.; OBJECTIVE: To evaluate T. gondii in blood, lung secretions [BAL], and cerebrospinal fluid specimens [CSF] obtained from AIDS patients with proven or suspected toxoplasmosis. METHODS: Samples were obtained from 12 AIDS patients (8 men, 4 women, age range 26-34 years, CD4+ cell counts
Modulation of Th1 responses in HIV infection and tuberculosis (TB).: Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0122). Unique Identifier : AIDSLINE ICA10/94369356; Barnes P; Zhang M; Jones B; Univ. of Southern California, Los Angeles.; To evaluate the Th1/Th2 cytokine profile in HIV-infected TB patients, we evaluated proliferative responses to M. tb and patterns of cytokine production by PBMC from 40 TB patients, with or without concomitant HIV infection. Upon stimulation with M. tb in vitro, PBMC from HIV-infected TB patients had reduced proliferat
Oral candidiasis in HIV infection--mucosal immunity versus strain selection.: Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0124). Unique Identifier : AIDSLINE ICA10/94369357; Challacombe SJ; Sweet SP; Dept. of Oral Medicine & Pathology, UMDS, Guy's Hospital, London,; UK.; OBJECTIVE: The aim of this study was to investigate the relative importance of host secretory immunity versus candidal pathogenicity in oral HIV-related candidiasis. METHODS: Whole and parotid saliva from HIV infected, AIDS and control subjects, and Candida sp. isolated from these groups, were compared. Five factors w
Serum cytokeratin 19 (CK 19) and pulmonary pneumocystosis (PCP) in HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0123). Unique Identifier : AIDSLINE ICA10/94369358; Amiel C; Dousset B; Rabaud C; May T; Canton P; Service de Maladies Infectieuses et Tropicales, CHU Nancy,; France.; Epithelial cells of the respiratory tract have been shown to express CK 19, a component of the cytoskeleton now widely considered as a characteristic and reliable tissue differentiation marker. As CK19 also exists as a secreted form, we measured circulating level in HIV+ patients, to investigate the usefulness of this
Activating effects of dioxin on HIV-1 in human CD4+ lymphoid cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):127 (abstract no. PA0126). Unique Identifier : AIDSLINE ICA10/94369359; Tsyrlov IB; Pokrovsky AG; NCI/NIH, Bethesda, MD 20892.; 2,3,7,8-TCDD (dioxin) and dioxin-like compounds are widely known because of their potential toxicity, including immunological disturbance the mechanism of which is still unknown. Here, effects of dioxin were assessed in regard to a marker enzyme CYP1A1 and HIV-1 genes. Dioxin and HIV-1 both affect the same target cell
FUO in HIV patients. A study of 83 episodes.: Int Conf AIDS. 1994 Aug 7-12;10(1):127 (abstract no. PA0127). Unique Identifier : AIDSLINE ICA10/94369360; Iribarren JA; Oyarbide I; Arrizabalaga J; Rodriguez F; Von Wichmann MA; Idigoras P; Mentxaka B; Unidad de Enf. Infecciosas, Hospital Aranzazu, San Sebastian,; Spain.; OBJECTIVES: To evaluate the etiology of FUO in patients with HIV infection (PWHIV). To study the utility of empiric antituberculosis therapy in patients with FUO and negative diagnostic investigations, in an area with high incidence of tuberculosis infection (Basque Country, Spain ).
Opportunistic infections in HIV-2 infected patients in Dakar (Senegal).: Int Conf AIDS. 1994 Aug 7-12;10(1):127 (abstract no. PA0128). Unique Identifier : AIDSLINE ICA10/94369361; Sow PS; Faye MA; Diouf G; Coll-Seck AM; Infectious Diseases Department, Fann Hospital, Dakar.; OBJECTIVES: (1) To identify the opportunistic infections in HIV-2 infected patients in Dakar. (2) To compare clinical manifestations in HIV-2 infected patients with HIV-1. METHODOLOGY: It is a case-control study from January 1986 to June 1993. Inclusion criteria were: patients with manifestations of HIV infection acco
Pulmonary infections of ARC/AIDS patients in the northeast of Thailand.: Int Conf AIDS. 1994 Aug 7-12;10(1):127 (abstract no. PA0129). Unique Identifier : AIDSLINE ICA10/94369362; Chetchotisakd P; Mootsikapun P; Intarapoka B; Department of Medicine, Faculty of Medicine, Khon Kaen; University, Thailand.; OBJECTIVE: To present etiologic pathogens of pulmonary infections in adult ARC/AIDS patients who were seen at Srinagarind hospital, tertiary care center in the Northeast of Thailand . METHOD: Data records of adult ARC/AIDS patients with respiratory symptoms and/or pulmonary infilt
Clonal deletion versus clonal anergy-facts and controversies.: Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0133). Unique Identifier : AIDSLINE ICA10/94369363; Salam A; Waer M; Vandeputte M; Rega Institute, Leuven, Belgium.; The present study was undertaken to investigate the role of viral superantigen in the process of clonal deletion and anergy in an immuno-suppressed state in an animal model in which M1sla acted as super antigen. HIV is reported recently to express a superantigenic characters which are responsible for clonal anergy and
Evidence for retroviral insertional mutagenesis in a subset of HIV-associated lymphomas.: Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0130). Unique Identifier : AIDSLINE ICA10/94369364; Herndier B; Shiramizu B; McGrath M; University of California, San Francisco 94110.; OBJECTIVE: To characterize on the molecular level a new class of HIV-associated lymphomas where the retrovirus HIV has inserted non-randomly into the tumor genomic DNA material. METHODS: 1) frozen section and paraffin section immunohistochemistry; 2) Southern blot analysis of inverse PCR products; 3) direct PCR sequen
Is the cofactor associated with Kaposi's sarcoma in HIV infected homosexual men declining over time?: Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0132). Unique Identifier : AIDSLINE ICA10/94369365; Veugelers PJ; Strathdee SA; Moss AR; Page KA; Tindall B; Schechter MT; Coutinho RA; van Griensven GJ; Municipal Health Service, Dept of Public Health, Amsterdam, The; Netherlands.; OBJECTIVE: A decline in the proportion of Kaposi s sarcoma (KS) among incident AIDS cases has been reported. This has led to suggestions regarding a decreasing exposure to an hypothesized agent. However, recent studies show that the incidences of KS has remained relatively stable since the beginning of the epidemic. W
Vascular endothelial growth factor produced by cultured AIDS-associated Kaposi's sarcoma spindle cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0131). Unique Identifier : AIDSLINE ICA10/94369366; Sakurada S; Mori KM; Salahuddin SZ; Okamoto T; Nakamura S; Dept. of Molecular Genetics, Nagoya City University, Japan.; OBJECTIVE: To elucidate the mechanism of edema in AIDS-associated Kaposi s sarcoma (AIDS-KS) lesions. METHODS: Long-term cultured AIDS-KS spindle cells and their semi-purified conditioned medium (AIDS-KS CM) through heparin sepharose affinity column were tested to detect the activity inducing vascular hyper-permeabili
Adaptation of human immunodeficiency virus type 2 (HIV-2) primary isolates to the in vivo replication in macaque.: Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0134). Unique Identifier : AIDSLINE ICA10/94369367; Wakrim L; Nicol I; Le Grand R; Boussin F; Vaslin B; Roques P; Bayon-Auboyer MH; Dormont D; Laboratoire de Neuropathologie experimentale et Neurovirologie; CEA, CEN/FAR, DSV/DPTE/SSA, Fontenay-Aux-Roses, France.; OBJECTIVES: To obtain a human immunodeficiency virus type 2 isolate (HIV-2) reproductively and persistently infectious for macaque aiming to generate a suitable animal model for vaccine and therapeutic strategies. METHODS: Two macaques (macaca fascicularis) H100 and 306A were intravenously inoculated with respectively
Further studies on the ability of lithium (Li) to minimize the blood cell toxicity of AZT.: Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0137). Unique Identifier : AIDSLINE ICA10/94369368; Gallicchio VS; Kazini S; Townsley E; Hughes NK; Tse KF; Scott KF; Lin J; Birch NJ; Division of Hematology/Oncology, University of Kentucky,; Lexington 40536.; OBJECTIVE: To further investigate the capacity of Li to influence the hematopoietic toxicity of AZT . METHODS: Normal mice (C57BL/6) received dose-escalation AZT (0.1 and 2.5 mg/ml, i.p.) respectively for four-weeks with added Li2CO3 (1 mM). Li was sto
Immune response and variation in provirus level during early phase of FIV-infected cats.: Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0136). Unique Identifier : AIDSLINE ICA10/94369369; Ohkura T; Iwa S; Tanabe-Tochikura A; Tamura T; Sato N; Kurimura T; Dept. of Pathology, Research Institute for Microbial Deseases,; Osaka University, Japan.; OBJECTIVE: To gain a better understanding of how the antibody in plasma and the provirus in PBMC vary in early phase of infection of SPF cats with FIV, we have analyzed the FIV and SPF cat system as an animal model of AIDS. METHODS: After i.v. inoculation of FIV (KYO-1 strain) to 5 SPF cats, the titer of antibody in p
Primary antibody response of human lymphocytes from SCID-hu mice injected with HIV1 peptides.: Int Conf AIDS. 1994 Aug 7-12;10(1):129 (abstract no. PA0135). Unique Identifier : AIDSLINE ICA10/94369370; Touraine JL; Chargui J; Desgranges C; Transplantation & Clinical Immunology Unit, INSERM U80, Lyon,; France.; OBJECTIVE: Severe combined immunodeficiency (SCID) mice can be transplanted with human lymphocytes (hu-PBL-SCID) or with human fetal liver, thymus and bone fragments (SCID-hu), thus providing a model for the study of HIV infection. This model has been used to investigate in vivo anti-HIV antibody response by human cel
Apoptosis induced in CD4+ cells expressing gp160 of HIV type 1.: Int Conf AIDS. 1994 Aug 7-12;10(1):13 (abstract no. 024A). Unique Identifier : AIDSLINE ICA10/94369371; Koga Y; Sasaki M; Department of Infectious Disease, Tohkai University School of; Medicine, Kanagawa, Japan.; CD4+ cell clones expressing gp160 or gp120 of HIV under the transcriptional control of an inducible promoter were made in order to examine the effect of these env products on the downregulation of surface CD4 and cell injury. A complete disappearance of surface CD4 preceding single-cell death occurred in the cell clon
Estimated production loss due to HIV/AIDS mortality in Canada, 1987-91.: Int Conf AIDS. 1994 Aug 7-12;10(1):13 (abstract no. 025D). Unique Identifier : AIDSLINE ICA10/94369372; Hanvelt R; Ruedy N; Hogg RS; Strathdee SA; O'Shaughnessy MV; Montaner JS; Schechter MT; BC Centre for Excellence in HIV/AIDS, Vancouver, Canada.; OBJECTIVE: To estimate and compare the impact of HIV/AIDS and other select causes of death on future production loss among Canadian men. METHODS: Data on all deaths for 1987-1991 attributed to HIV/AIDS and other select causes were obtained from Statistics Canada . Future production
Measuring the impact of HIV/AIDS on Africa's commercial sector.: Int Conf AIDS. 1994 Aug 7-12;10(1):13 (abstract no. 026D). Unique Identifier : AIDSLINE ICA10/94369373; Forsythe S; Roberts M; Wongombe J; Gakuru O; AIDSCAP/FHI, Arlington, VA.; OBJECTIVE: The objective of this assessment was to determine how the commercial sector in Africa is currently being affected by HIV/AIDS and to project how this impact may change over time. This analysis was performed in order to create greater awareness among business managers and policymakers within the commercial s
The impact of HIV/AIDS on the education system.: Int Conf AIDS. 1994 Aug 7-12;10(1):13 (abstract no. 027D). Unique Identifier : AIDSLINE ICA10/94369374; Shaeffer S; UNICEF, Bangkok, Thailand.; OBJECTIVE: To identify ways in which HIV/AIDS is having, and will continue to have, an impact: (1) on the demand, supply, and quality of education; and (2) on education systems objectives and functions, on what they teach, and on the nature of their planning, financing, and management processes. METHODS: An extensive
A trial to establish a novel mouse model for AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):130 (abstract no. PA0141). Unique Identifier : AIDSLINE ICA10/94369375; Yoshimoto T; Nagase H; Kojima K; Kanamori Y; Nariuchi H; Department of Allergology, Institute of Medical Science,; University of Tokyo, Japan.; OBJECTIVE: To try to establish a novel mouse model for AIDS, we analyzed various immunological parameters of DDD/1 mice subcutaneously injected with a mouse mammary tumor virus, MMTV (FM), which we have recently isolated and has strong superantigenic activity reacting to V beta 8.2+ T cells. METHODS: After subcutaneou
Mechanisms of 2'3'-dideoxycytidine-induced murine thymic lymphoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):130 (abstract no. PA0138). Unique Identifier : AIDSLINE ICA10/94369376; Irons RD; Le AT; Stillman WS; University of Colorado Health Sciences Center Molecular; Toxicology & Environmental Health Sciences Program, Denver 80262.; The nucleoside analoque, 2 ,3 -dideoxycytidine ( ddC ), is a potent inhibitor of human immunodeficiency virus reverse transcriptase that exhibits a different toxicity profile than zidovudine (
The effect of glycyrrhizin in mice infected with murine retrovirus. I. Prevention of the progression of disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):130 (abstract no. PA0139). Unique Identifier : AIDSLINE ICA10/94369377; Miyaji C; Watanabe H; Makino M; Abo T; Dep. of Immunol., Niigata Univ. Sch. of Medicine, Japan.; OBJECTIVE: Glycyrrhizin (GL), one of the plant extracts, have been evaluated for their inhibitory effect on HIV replication. From these observations, we attempted to use GL in murine AIDS (MAIDS) model and evaluated the development of MAIDS. METHODS: C57BL/6 mice were inoculated with LP-BM5 MuLV. Treatment with GL was
The effect of glycyrrhizin in mice infected with murine retrovirus. II. Inhibitory effects on proliferation and function of lymphoid cells.: Int Conf AIDS. 1994 Aug 7-12;10(1):130 (abstract no. PA0140). Unique Identifier : AIDSLINE ICA10/94369378; Watanabe H; Miyaji C; Makino M; Abo T; Dep. of Immuno., Niigata Univ. Sch. of Med., Japan.; OBJECTIVE: Glycyrrhizin (GL) was found to be effective in HIV infected patients as shown by improvements of their clinical symptoms and of their immunological and liver function. GL activated selectively extrathymic T cells in the liver, and it is suspected that this phenomenon might be intimately related to such the
Dynamics of CD4 and CD8 cells in blood and lymph nodes of SIVmac239 infected monkeys.: Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0145). Unique Identifier : AIDSLINE ICA10/94369379; Murayama Y; Mukai R; Matui K; Sata T; Takasaka M; Noguchi A; Hassan MA; Hayami M; Yoshikawa Y; Tsukuba Primate Center for Medical Research, NIH, Japan.; OBJECTIVE: The purpose of the investigation was to analyze the dynamic interaction between disease progression and lymphocyte subsets in the blood and lymph nodes (LN). METHODS: The time courses of changes in CD4 subsets and CD4/CD8 ratios were compared in bioptic samples of the LN and blood from SIVmac239 infected rh
Mycobacterium avium infection and murine AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0142). Unique Identifier : AIDSLINE ICA10/94369380; Perronne C; Grassi F; Levacher M; Cohen Y; Maslo C; Chau F; Sinet M; Vilde JL; Pocidalo JJ; Service des Maladies Infectieuses, Hopital Bichat-Claude; Bernard, Paris, France.; OBJECTIVE: To study in mice the interaction between a retroviral infection (murine AIDS) and Mycobacterium avium complex (MAC) infection. METHODS: The murine leukemia virus LP-BM5 was inoculated to C57BL/6 mice by i.v. injection either 8 weeks before an i.v. challenge with 10(3) or 10(6) MAC (strain MO-1) (coinfection
Sequence necessary for the pathogenicity of murine AIDS (MAIDS) virus.: Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0143). Unique Identifier : AIDSLINE ICA10/94369381; Ishimoto A; Kubo Y; Higo K; Kobayashi H; Hirama T; Institute for Virus Research, Kyoto University, Japan.; OBJECTIVE: It was examined whether the unique sequences of defective murine AIDS virus in its gag p15 and p12 regions are responsible for the development of MAIDS. METHODS: Recombinant viruses by replacing various regions of the gag gene of nonpathogenic ecotropic virus, with those of the MAIDS virus were constructed
SIVmac chimeric viruses having HIV-1 env useful for HIV-1 vaccine evaluation using macaque monkeys.: Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0144). Unique Identifier : AIDSLINE ICA10/94369382; Kuwata T; Igarashi T; Okada M; Ido E; Ami Y; Komatsu T; Jin M; Adachi A; Hayami M; Institute for Virus Research, Kyoto University, Japan.; OBJECTIVE: By constructing SIVmac chimeric viruses having HIV-1 env more efficient in macaque infection, we attempts to establish an experimental infection system using macaque monkeys instead of chimpanzees. METHODS: The chimeric viruses having LTRs, gag, pol, vif and vpx of SIVmac and tat, rev, vpu and env of HIV-1
Involvement of B cell subpopulation in progression of murine retrovirus-induced immunodeficiency syndrome.: Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0147). Unique Identifier : AIDSLINE ICA10/94369383; Hitoshi Y; Numata F; Uehara S; Takatsu K; Dept. Immunol., Univ. Tokyo, Japan.; OBJECTIVE, Infection of susceptible C57BL/6 (B6) mice with LP-BM5 MuLV leads to the development of the murine acquired immunodeficiency syndrome (MAIDS), which shows many similarities with human AIDS. It has been reported that B cells are required for the progression of MAIDS. In our previous study, X chromosome-linke
Involvement of IFN-gamma in the progression of the murine acquired immunodeficiency syndrome.: Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0148). Unique Identifier : AIDSLINE ICA10/94369384; Uehara S; Hitoshi Y; Numata F; Takatsu K; Dep. Immunol., Sci., Univ. Tokyo, Japan.; OBJECTIVE: The murine acquired immunodeficiency syndrome (MAIDS) caused by defective LP-BM5 murine leukemia viruses (MuLV) is a disease that shows progressive splenomegary, severe immunodeficiency and hypergammaglobulinemia. The onset of MAIDS requires the presence of CD4+ T cells and B cells, and the development of M
Maintenance of high virus load even after seroconversion in newborn cats acutely infected with feline immunodeficiency virus.: Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0149). Unique Identifier : AIDSLINE ICA10/94369385; Zheng YH; Tokunaga K; Shoda K; Nishino Y; Kishi M; Zhong Q; Asahi S; Ishihara C; Kanda M; Ikuta K; Inst. Immunol. Sci., Hokkaido Univ., Sapporo, Japan.; OBJECTIVE: As an animal model for HIV pediatric infection, we examined whether or not the time at which viral infection occurs is an important factor for FIV-induced pathogenesis. METHODS: Three newborn and two adult cats were intraperitoneally inoculated with FIV Petaluma strain-infected feline T-lymphoblastoid cells
The growth potency and cytopathogenicity of recombinant chimeric viruses between HIV-1 and SIVagm.: Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0146). Unique Identifier : AIDSLINE ICA10/94369386; Jin MH; Ido E; Kuwata T; Igarashi T; Okada M; Cichutek K; Kurth R; Miura T; Hayami M; Inst. for Virus Res., Kyoto Univ., Japan.; OBJECTIVE: We attempted to identify the viral determinant for pathogenicity by constructing recombinant chimeric viruses between HIV-1 and a non-pathogenic SIVagm originally isolated from African green monkeys. METHODS: Starting from two infectious DNA clones, pNL432 (HIV-1) and pSIVagm3 (SIVagm3), two recombinant chi
Epidemiological study on HTLV-II infection among Colombian native indians.: Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0153). Unique Identifier : AIDSLINE ICA10/94369387; Fujiyama C; Fujiyoshi T; Yashiki S; Tajima K; Hayami M; Zaninovic V; Sonoda S; Department of Virology, Faculty of Medicine, Kagoshima; University, Japan.; OBJECTIVE: Co-infection with HIV and human T-cell lymphotropic virus type II (HTLV-II) is known to be prevalent among intravenous drug users (IVDUs). There are some reports indicating that HTLV-II may modify the HIV infection, while it is not clear the natural history of HTLV-II and effects of HTLV-II on prognosis of
Evaluation of immunoassays for detection of HTLV-I/II antibodies.: Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0151). Unique Identifier : AIDSLINE ICA10/94369388; Andersson S; Godoy K; Thorstensson R; Dias F; Biberfeld G; Dept. of Immunology, Swedish Institute for Infectious Disease; Control, Stockholm, Sweden.; BACKGROUND: A general screening of blood donors for HTLV-I/II infection during one year from the beginning of 1994 has been initiated in Sweden . OBJECTIVE: To assess the sensitivity and specificity of commercially available screening assays for HTLV-I/II antibodies. METHODS: Serum
HIV/HTLV western blot cross reactivity.: Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0150). Unique Identifier : AIDSLINE ICA10/94369389; Constantine NT; Jones KS; Zhang X; Abdel-Hamid M; Dept. Pathology, Univ. of Maryland Sch. of Med., Baltimore 21201.; OBJECTIVES: To assess the cross reactivity between HIV and HTLV by Western blots (WB). METHODS: 100 sera, 92 classified as HIV inconclusive (ELISA reactive but not confirmed by HIV WB), and 8 WB+ were tested using one of two HTLV WB (1.2, Diagnostic Biotechnology; or HTLV-I/II, Cambridge Biotech). 30 sera had been neg
Prevalence of HTLV-I/II antibodies among HIV-1-asymptomatic patients in Sao Paulo, Brazil.: Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0152). Unique Identifier : AIDSLINE ICA10/94369390; Casseb JS; Caterino-de-Araujo A; Duarte AJ; Inst. de Infect. Emilio Ribas, Sao Paulo, Brazil.; OBJECTIVE: To determine the seroprevalence of HTLV-I/II antibodies in HIV-1-asymptomatic subjects, and identify the major route of contamination with these dual retroviruses. PATIENTS AND METHODS: A cohort of 123 HIV-1-infected individuals in ambulatorial accompaniment to study the natural HIV history at the Secondary
Evaluation of HTLV-I infection in healthy Nikkei population of Lima-Peru.: Int Conf AIDS. 1994 Aug 7-12;10(1):134 (abstract no. PA0155). Unique Identifier : AIDSLINE ICA10/94369391; Gotuzzo E; Yamamoto V; Kanna M; Chauca G; Philipps I; Watts D; AvH, UPCH, Lima-Peru.; HTLV-I infection has been recognized as endemic in Japan , the Caribbean and recently in Colombia and Peru . Th
HTLV-I among native people of far eastern regions of Russia.: Int Conf AIDS. 1994 Aug 7-12;10(1):134 (abstract no. PA0156). Unique Identifier : AIDSLINE ICA10/94369392; Gurtsevitch V; Senyuta N; Stepina V; Yakovleva L; Syrcev A; Susova O; Pavlish O; Cancer Research Center, Moscow, Russia.; OBJECTIVE: To study HTLV-I prevalence among native people inhabiting Far Eastern Russia with special attention to Nivkhi people from Sakhalin intimately connected with Ainu people before 1945. The latters revealed high level of HTLV-I seroprevalence when have been tested in Hokkaid
HTLV-I infection in healthy blood donors in Russia.: Int Conf AIDS. 1994 Aug 7-12;10(1):134 (abstract no. PA0157). Unique Identifier : AIDSLINE ICA10/94369393; Parfanovich M; Zuntova Yu; Vlshemirsky O; Plvovarova I; D.I. Ivanovsky Institute of Virology, Moscow, Russia.; 1176 sera from the patients with the different forms of haemoblastosis, blood donors, people from Chernobyl-region, patients with haemophilia were studied in 1993. The presence of antibodies to HTLV-I in sera was determined by indirect ELISA on the basis of natural viral antigen and was confirmed by Western blot Du Po
Tropical spastic paraparesis and HTLV-I infection on the Caribbean Coast of Colombia.: Int Conf AIDS. 1994 Aug 7-12;10(1):134 (abstract no. PA0154). Unique Identifier : AIDSLINE ICA10/94369394; Pareja J; Daza J; Boshell J; Rojas MC; Gonzalez M; Marrugo S; Mc Allister; Navas MC; Jeannel D; Universidad de Cartagena.; OBJECTIVE: To assess the presence of clinical cases of Tropical Spastic Paraparesis/Human Associated Myelopathy (TSP/HAM) and serological evidence of HTLV-I infection on the Caribbean Coast of Colombia with a population of african a
Evidence for HTLV-1 infection in Uganda.: Int Conf AIDS. 1994 Aug 7-12;10(1):135 (abstract no. PA0159). Unique Identifier : AIDSLINE ICA10/94369395; Biryahwaho B; Downing RG; de The G; UVRI, Entebbe & Pasteur Institute, Paris.; OBJECTIVE: To determine the seroprevalence of HTLV-1 in Uganda . METHODS: Specimens for national HIV sentinel surveillance were collected from 5 antenatal clinics in 1992 as follows: Kampala, Kasese, Kisoro, Moroto and Moyo. 425 specimens were selected at random and tested for antib
HTLV-I/II infection among French blood donors.: Int Conf AIDS. 1994 Aug 7-12;10(1):135 (abstract no. PA0158). Unique Identifier : AIDSLINE ICA10/94369396; Pillonel J; Courouce AM; Lemaire JM; Brunet JB; National Network of Public Health, France.; OBJECTIVES: 1) to determine the prevalence of HTLV-I/II antibodies among French blood donors (BDs) since the beginning of the systematic screening (15th July 1991) in Continental France (CF). 2) to study the epidemiological characteristics of HTLV-I/II seropositive BDs. METHOD: Sin
Rat model of HTLV-I infection--morphological study of HAM rat disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):135 (abstract no. PA0160). Unique Identifier : AIDSLINE ICA10/94369397; Abe M; Fukatsu R; Seto K; Tomaru U; Ohya O; Kasai T; Yamashita I; Ikeda H; Wakisaka A; Yoshiki T; Dept. Pathol., Hokkaido Univ. Sch. Med., Japan.; OBJECTIVE: To analyse mechanisms involved in the development of HTLV-I-associated diseases, animal models have long been needed. Here we show a rat model of HAM/TSP in humans, designated as HAM rat disease, which develops a chronic progressive myelopathy associated with HTLV-I infection after 16 months of incubation.
Roles of HTLV-1 and genetic background in rat model of HAM/TSP.: Int Conf AIDS. 1994 Aug 7-12;10(1):135 (abstract no. PA0161). Unique Identifier : AIDSLINE ICA10/94369398; Kushida S; Mizusawa H; Tanaka H; Ami Y; Hori M; Yagami K; Kameyama T; Yoshida A; Nyunoya H; Iwasaki Y; et al; Institute of Basic Medical Sciences, University of Tsukuba,; Ibaraki, Japan.; OBJECTIVE: Clarification of the roles of Human T-cell leukemia virus type-1 (HTLV-1) in HTLV-1-associated myelopathy (HAM)/tropical spastic paraparesis (TSP) with rat model of HAM/TSP. METHODS: HTLV-1-producing T cells were injected intravenously into WKA and F344 rats. Detection of HTLV-1 provirus in peripheral blood
Characterization of enhancer-binding proteins that recognize HTLV-I LTR.: Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0165). Unique Identifier : AIDSLINE ICA10/94369399; Nyunoya H; Shimotohno K; National Cancer Center Research Institute, Tokyo, Japan.; OBJECTIVE: The promoter activity of HTLV-I can be controlled by tax-gene product as well as cyclic AMP or TPA. Factors related to the cellular signal-transduction pathways may be involved in the transcriptional control. To identify such factors, we have cloned cDNAs for multiple enhancer-binding proteins and character
Genetic analyses of HTLV-I from India and newly identified endemic Loci, Iranian Mashhad and Russia.: Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0162). Unique Identifier : AIDSLINE ICA10/94369400; Yamashita M; Syrtsev A; Achiron A; Shohat B; Gurtsevitch V; Ishida T; Yamamoto N; Takehisa J; Igarashi T; Ibuki K; et al; Inst. Virus Res., Kyoto Univ., Japan.; OBJECTIVE: Recently, Mashhadi Jews immigrated from Iran to Israel and people in some part of Russia such as Saharin and Turkmenistan were reported to have high prevale
Latent infection of human T-cell leukemia type I (HTLV-I) in human monocytic cell (THP-1).: Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0164). Unique Identifier : AIDSLINE ICA10/94369401; Ohara Y; Iwasaki Y; Terunuma H; Tsunoda I; Mizobuchi M; Department of Neurological Sciences, Tohoku University School of; Medicine, Sendai, Japan.; OBJECTIVE: Besides CD4-positive lymphocytes, monocyte/macrophage (M/M) lineage cells have been shown to be infected with HTLV-I. Moreover, in the rat CNS disease induced by inoculation of HTLV-I carrying lymphoid cells, cellular infiltrates in the CNS are exclusively mononuclear cells of M/M lineage. For further eluci
Phylogenetic analysis of HTLV-I/II from Colombia, Chile and Ghana.: Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0163). Unique Identifier : AIDSLINE ICA10/94369402; Miura T; Zaninovic V; Cartier L; Osei-Kwasi M; Aysi NK; Tajima K; Sonoda S; Yamashita M; Igarashi T; Takehisa J; et al; Inst. for Virus Res., Kyoto Univ., Japan.; OBJECTIVE: To clarify the phylogenetic relation of HTLV in the world, some new isolates of HTLV-I/II from Colombia , Chile and Ghana were genetically analyzed an
Expression of HTLV-I rex and tax recombinant proteins in E. coli.: Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0167). Unique Identifier : AIDSLINE ICA10/94369403; Susova O; Pavlish O; Scherbak L; Gurtsevitch V; Cancer Research Center, Moscow, Russia.; OBJECTIVE: HTLV-I has been known to contain specific regulatory genes rex and tax. The aim of the investigation was to obtain and to characterize recombinant proteins encoded by these genes in bacterial cells. METHODS: Routine gene-engeneering and biotechnological procedures were used to accomplish molecular cloning f
Inverse correlation between viremia and natural killer cells in a naturally SIVcpz-ant infected chimpanzee.: Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0169). Unique Identifier : AIDSLINE ICA10/94369404; Kestens L; Vingerhoets J; Peeters M; Vanham G; van der Groen G; Gigase P; Heeney J; Inst. of Trop. Med., Antwerpen, Belgium.; OBJECTIVE: 1) To analyze parameters of cellular immunity in a wild-caught chimpanzee, naturally infected with an HIV-1 related lentivirus (SIVcpz-ant). 2) To compare results of 36 months of follow-up with 3 experimentally HIV-1 infected and 5 uninfected control chimpanzees. METHODS: Quantitative virus cultures from pe
Properties of a defective SIVsm virus clone.: Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0168). Unique Identifier : AIDSLINE ICA10/94369405; Kraiselburd E; Malaga C; Beltran M; Leung N; Torres J; UPR Med Sch.; OBJECTIVE: To determine the properties of Viruses produced by an SIVsm chronically infected cell line. METHODS: Particles were examined by e.m., by infectivity assays (in vitro and in vivo) and by immunoblots. Nucleic acid was partially sequenced. RESULTS: Defective particles were immature. The contained env and gag p
Protein tyrosine phosphorylation in HTLV-1 infected and Tax1-transduced T lymphocytes.: Int Conf AIDS. 1994 Aug 7-12;10(1):137 (abstract no. PA0166). Unique Identifier : AIDSLINE ICA10/94369406; Akagi T; Tono H; Shimotohno K; Virology Division, National Cancer Center Research Institute,; Tokyo, Japan.; To elucidate the mechanism of abnormal growth of HTLV-1 infected T lymphocytes, we have investigated the protein tyrosine-phosphorylation, which is known to play an important role in growth-signal transduction, by the western blot analysis using anti-phosphotyrosine antibody. HTLV-1 infected T cell clone showed signif
Analysis of Gag protein region involved in feline immunodeficiency virus particle formation.: Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0171). Unique Identifier : AIDSLINE ICA10/94369407; Morikawa S; Kitamura T; National Institute of Health, Tokyo, Japan.; OBJECTIVE: To elucidate the functional domains required for proper assembly to Gag precursor proteins of feline immunodeficiency virus (FIV). METHODS: A variety of deletions were introduced within the FIV gag gene. Several chimeric gag genes were constructed between FIV and simian immunodeficiency virus (SIV). These r
Functional analysis of retroviral env peptide recognized by T helper clones.: Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0172). Unique Identifier : AIDSLINE ICA10/94369408; Yamagishi H; Shimizu T; Uenishi H; Teramura Y; Iwashiro M; Kuribayashi K; Dept. Biophysics, Fac. Science, Kyoto University, Japan.; OBJECTIVE: We studied peptide-MHC interactions and their subsequent recognition by murine retroviral env122-141 specific Th cells. METHODS: We used the target peptide of decreasing length to define the preferred size of peptide and the variant peptide with single alanine substitutions to identify key amino acids requi
Increased risk for a second retroviral infection (SIV or STLV) for wild monkeys already infected by one retrovirus.: Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0170). Unique Identifier : AIDSLINE ICA10/94369409; Tuppin P; Durand JP; Maison P; Galat G; Galat-Luong A; Jeannel D; de The G; Unite d'Epidemiologie des Virus Oncogenes, Institut Pasteur,; Paris, France.; In Senegal , between 1981 and 1982, 215 monkeys were killed during a yellow fever control in Kedougou, and 177 were trapped between 1989 and 1991 in Sine Saloum. There were 221 patas monkeys (PM) and 171 african green monkeys (AGM). Sera were screened for SIV by ELISA assay (HIV 1
Splicing signals are required for the expression of unspliced Gag-containing message of moloney murine leukemia virus.: Int Conf AIDS. 1994 Aug 7-12;10(1):138 (abstract no. PA0173). Unique Identifier : AIDSLINE ICA10/94369410; Odawara T; Ohshima M; Matano T; Iwamoto A; Yoshikura H; Faculty of Medicine, University of Tokyo, Japan.; OBJECTIVE: All the retroviruses express both unspliced and spliced messages simultaneously. However, little is known about the control of the production of the unspliced message under the presence of splicing. We studied the role of splicing signals on the expression of a retroviral unspliced message. METHODS: A Molon
A study of viral load in HIV-2 infection in a rural community of west Africa.: Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0174). Unique Identifier : AIDSLINE ICA10/94369411; Ariyoshi K; Berry N; Wilkins A; Ngom PT; Tedder RS; Whittle H; M.R.C. Lab., Gambia, West Africa.; OBJECTIVES: To determine HIV-2 proviral load in seropositive individuals in a rural village in Guinea-Bissau and to investigate the effects of aging and co-infection with HTLV-I, malaria and syphilis. METHODS: DNA samples from 88 subjects were investigated for quantitative PCR wit
Is there any receptor other than CD4 essential for HIV-1 infection?: Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0175). Unique Identifier : AIDSLINE ICA10/94369412; Furlini G; Re MC; Vignoli M; Ramazzotti E; Monari P; La Placa M; Institute of Microbiology, University of Bologna, St. Orsola; Hospital, Italy.; OBJECTIVE: The present work aimed to elucidate whether human hematopoietic progenitor cells show substantial and significant difference on the presence of membrane molecules in comparison with HIV-1 susceptible cell lines, to verify whether the lack of infectability of progenitor cells, despite CD4 presence, may be re
Possible role of pregnancy-specific glycoprotein (PSG) in mother-child HIV infection transfer.: Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0177). Unique Identifier : AIDSLINE ICA10/94369413; Blinov VM; Resenchuk SM; Chirikova GB; Denisov SI; Zverev VV; Institute of Molecular Biology, Koltsovo, Novosibirsk, Russia.; More than 3 million women mostly in reproductive age were AIDS-infected in 1990. Vertical transfer of HIV from an infected pregnant woman to her child becomes the main source of AIDS patients. Revealing of those protective factors which provide the birth of a healthy child from the HIV-infected mother is one of the mo
The role of human CD4 and CD26 in HIV infection of murine and rabbit transfectants.: Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0176). Unique Identifier : AIDSLINE ICA10/94369414; Yamamura Y; Ikawa Y; Dept. Biochemistry, Tokyo Medical & Dental Univ. Sch. Medicine,; Japan.; OBJECTIVE: Human CD4 is essential for HIV binding but not for HIV entry into the cells to establish infection. Although the human CD26 molecule was recently reported to be a cofactor that helps HIV infection, it is unclear whether only human CD4 and CD26 are sufficient for HIV infection. We evaluate the roles of human
AIDS mortality in Mexico: 1983-92. Years of potential productive life lost.: Int Conf AIDS. 1994 Aug 7-12;10(1):14 (abstract no. 028D). Unique Identifier : AIDSLINE ICA10/94369415; Izazola-Licea JA; Valdez-Garcia M; del Rio C; CONADISA, Mexico.; OBJECTIVE. To estimate the cumulative number of years of potential productive life lost (YPPLL) due to AIDS in Mexico , and its distribution by occupation. METHODS. Sixty-five years was considered the upper limit of potential productive life in men and 60 in women. The distribution
HIV mortality and the impact on social benefits given to employees family.: Int Conf AIDS. 1994 Aug 7-12;10(1):14 (abstract no. 029D). Unique Identifier : AIDSLINE ICA10/94369416; Mposo N; Bertozzi S; Doppagne A; Khondi N; Engele B; Wandu M; Nzila N; Projet Sida Kinshasa, Zaire.; OBJECTIVES: To understand if early death due to HIV infection has any impact on social benefits provide to employees family on private sector. METHODS: We used a previously identified cohort of HIV positive (ELISA/Western Blot) employees (n = 90) and selected a seronegative control cohort of equal size, individually m
The fire in the dragon: a study of risk factors for HIV/AIDS among urban men and commercial sex workers in Vietnam.: Int Conf AIDS. 1994 Aug 7-12;10(1):14 (abstract no. 031D). Unique Identifier : AIDSLINE ICA10/94369417; Franklin BA; Khanh NT; CARE International, Vietnam.; OBJECTIVE: To identify risk factors for sexual transmission of HIV/AIDS in Vietnam through a study of commercial sex workers and urban men. to guide policy planners and communicators at the onset of the epidemic in Vietnam. METHODS: A 4-tiered, sequenced research design was used t
Why isn't increased survival enough?: Int Conf AIDS. 1994 Aug 7-12;10(1):14 (abstract no. 030D). Unique Identifier : AIDSLINE ICA10/94369418; Wilson AD; Wellcome Foundation Ltd, Beckenham, Kent.; This presentation will review the four main reasons for applying economic analyses to the field of HIV and AIDS healthcare. These are: 1. Current projections of the growing number of people who may be affected by HIV/AIDS worldwide. 2. The predicted large degree of dependence on healthcare systems of people with HIV/A
Enhancement of HIV-1 expression with lipopolisaccharide (LPS).: Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0178). Unique Identifier : AIDSLINE ICA10/94369419; Sanuma K; Chowdhury H; Matsuda K; Koyanagi Y; Yamamoto N; Department of Microbiology, Tokyo Medical and Dental University.; OBJECTIVE: Using CD14-positive HIV-1 infected cell line, J22HL60, we try to find out the mechanism of HIV expression induced by LPS in macrophage. METHODS: Induction of J22HL60 cells was proceeded for HIV expression with or without inhibitors. HIV expression was detected by indirect immunofluorescent staining and P24
Lipids of Mycoplasma fermentans have an activity of HIV induction.: Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0180). Unique Identifier : AIDSLINE ICA10/94369420; Matsuda K; Sanuma K; Taki T; Handa S; Yamamoto N; Harasawa R; Tokyo Med. and Dent. Univ., Japan.; OBJECTIVE: Mycoplasma fermentans is suspected as a cofactor of AIDS, but the mechanism of pathogenesis is not well known. We purified and characterized novel phosphorylcholine-containing glycoglycerolipid from HIV- and HTLV-I-infected human helper T-cell culture, and the lipids were found to be from accidentally infec
Role of mycoplasma in SIV infection of rhesus macaques.: Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0181). Unique Identifier : AIDSLINE ICA10/94369421; Bahraoui E; Kovacic R; Canarelli S; Guetard D; Hurtrel B; Blanchard A; Montagnier L; URA 1455 CNRS, Marseille.; OBJECTIVE: Here, using rhesus macaque as a model, we investigated the susceptibility of these animals to mycoplasma infection and the role of Mycoplasma penetrans, Mycoplasma pirum and Mycoplasma fermentans in simian immunodeficiency virus (SIV) replication and pathogenicity. MATERIALS AND METHODS: Rhesus macaques wer
Superantigen TSST-1 enhances the replication of HIV-1 in PBMCs through selective activation of CD4+ T-cell and induction of TNF-alpha.: Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0179). Unique Identifier : AIDSLINE ICA10/94369422; Hashimoto K; Baba M; Shigeta S; Department of Microbiology, Fukushima Medical College, Japan.; OBJECTIVE: Staphylococcal superantigens bind to the class II molecules expressed on the surface of peripheral blood mononuclear cells (PBMCs) and potently activate them. Tumor necrosis factor alpha (TNF-alpha) plays an essential role in human immunodeficiency virus type 1(HIV-1) infection. In addition, the superantige
Comparison of human herpesvirus-6 (HHV-6) variant A and B genes.: Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0182). Unique Identifier : AIDSLINE ICA10/94369423; Yamanishi K; Yamamoto T; Isegawa Y; Mukai T; Osaka University, Japan.; OBJECTIVE: HHV-6 is now classifird into two variants such as HHV-6A and HHV-6B. The gene product of putative immediate-early(IE) loci in HHV-6 variant A was reported to transactivate human immunodeficiency virus (HIV). Here we report the comparison of sequences in strain HST, variant B isolated from a Japanese patient
HIV 'tat' gene is homologous with regions in HSV and EBV suggesting possible basis for synergism.: Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0185). Unique Identifier : AIDSLINE ICA10/94369424; Smith RW; Inst. for Postgrad. Interdisc. Stud., Palo Alto, CA 94306-0846.; OBJECTIVE: Synergism among lentiviruses and herpesviruses (especially gamma herpesviruses) is well known but poorly understood. One report of superinfections with lentiviruses and herpesviruses suggests that herpesviruses may activate lentiviruses. We reported that tat and 5 other HIV regulatory genes may account for
Inhibition of human herpesvirus 7 infection by anti-CD4 monoclonal antibodies and HIV-1 gp120.: Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0184). Unique Identifier : AIDSLINE ICA10/94369425; Yasukawa M; Yakushijin Y; Furukawa M; Hato T; Takada K; Fujita S; First Dep. Int. Med., Ehime University School of Medicine, Japan.; OBJECTIVE: We have recently found that human herpesvirus (HHV)-7 shows tropism to CD4+ T cells, but not to CD4- cells, and that the transport of intracytoplasmic CD4 molecules to the cell surface is impaired in HHV-7-infected cells (J. Immunol. in press). Based on these findings, we speculated that the CD4 molecule mi
Interaction of HIV-1 and HHV-6 in human T-cell line.: Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0183). Unique Identifier : AIDSLINE ICA10/94369426; Takasaki T; Yamaguchi J; Ohkawa N; Sano K; Nakano T; Nakai M; Dept. of Bacteriology, Kinki Univ. School of Medicine, Osaka,; Japan.; OBJECTIVE: This study morphologically investigates the interaction of HIV-1 and HHV-6 in human T-cell line. METHODS: HIV-1 was inoculated to Molt-4 and MT-4 cells infected with HHV-6 in 7 days after HHV-6 infection, and continued to culture for another 7 and 10 days. The infection of HIV-1 and HHV-6 were monitered by
Chlamydial conjunctivitis associated with sexually transmitted diseases.: Int Conf AIDS. 1994 Aug 7-12;10(1):142 (abstract no. PA0188). Unique Identifier : AIDSLINE ICA10/94369427; Suzuki K; Numazaki K; Aoki K; Isobe K; Ohno S; Chiba S; Department of Pediatrics, Sapporo Medical University School of; Medicine, Japan.; OBJECTIVE: A part of chlamydial infections is known to be sexually transmitted. If one person develops chlamydial conjunctivitis, the sexual partner also has a high chance of developing it. The objective of this study to examine the clinical and microbiological features of chlamydial conjunctivitis among the sex partn
Host immune response in chlamydial cervicitis.: Int Conf AIDS. 1994 Aug 7-12;10(1):142 (abstract no. PA0189). Unique Identifier : AIDSLINE ICA10/94369428; Mittal A; Kapur S; Institute of Pathology, New Delhi, India.; OBJECTIVE: To study host immune responses in chlamydial cervicitis. METHODS: Cellular immune responses were studied in peripheral blood and compared with local immune responses to chlamydial elementary bodies in patients. RESULTS: No significant difference was noted in lymphoblast transformation to antigens (PPD and C
Interaction of HIV and human herpesvirus 7 in a CD4+ T cell line.: Int Conf AIDS. 1994 Aug 7-12;10(1):142 (abstract no. PA0186). Unique Identifier : AIDSLINE ICA10/94369429; Yamada M; Kamiya T; Yoshida M; Matsuo Y; Ikuta K; Yamanishi K; Okuno T; Minowada J; Nii S; Dep. of Virol., Okayama Univ. Med. Sch., Japan.; OBJECTIVE: Human Herpesvirus 7 (HHV-7), a new T-lymphotropic herpesvirus, is related to, but significantly different from HHV-6. HIV-1 and HHV-7, as well as HHV-6, are believed to share CD4+ T lymphocytes as major target cells, and, for this reason, we studied the interaction of HIV-1 and HHV-7 using a CD4+ T cell lin
Replication of Chlamydia trachomatis and Chlamydia pneumoniae in human monocytic cell line U-937.: Int Conf AIDS. 1994 Aug 7-12;10(1):142 (abstract no. PA0187). Unique Identifier : AIDSLINE ICA10/94369430; Numazaki K; Suzuki K; Chiba S; Department of Pediatrics, School of Medicine, Sapporo Medical; University, Japan.; OBJECTIVE: Despite the various pathogenic effects of Chlamydiae, there is only limited direct evidence that chlamydia infections occur in monocytes and macrophages. The present study was designed to determine the effects of C. trachomatis and C. pneumoniae on monocytes-macrophages and the mechanisms of persistence in
Laboratory findings for the prevalence of HIV and other STDS in antenatal attendees at Mnazi Mmoja Hospital: a pilot study.: Int Conf AIDS. 1994 Aug 7-12;10(1):143 (abstract no. PA0190). Unique Identifier : AIDSLINE ICA10/94369431; Othman AA; Shein AM; Ali AK; AIDS Control Programme, Zanzibar.; OBJECTIVE: To determine the rate of STDs in antenatal clinic. METHOD: Vaginal swabs and blood were collected from ANC attendees at Mnazi mmoja referal hospital between Jan-Dec 1993. Thayer and Martin agar was used for the isolation of N.gonorrhoea, EIAs for HIV antibody detection and TPHA for syphilis. RESULTS: A tota
Demographics and survival in urban adults with HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):144 (abstract no. PB0001). Unique Identifier : AIDSLINE ICA10/94369432; Chaisson RE; Keruly J; Moore RD; Johns Hopkins Univ., Baltimore, MD.; OBJECTIVE: To compare survival patterns in a demographically mixed population of patients with HIV disease from an urban area. METHODS: Patients registered in an urban HIV Clinic were followed observationally for a mean of 15 months (median 12) from entry. Survival was analyzed by selected demographic factors and CD4
Does Pneumocystis carinii prophylaxis modify the clinical and biological AIDS presentation? Results of the Bordeaux Hospital cohort (France, 1985-1993). The GECSA.: Int Conf AIDS. 1994 Aug 7-12;10(1):144 (abstract no. PB0004). Unique Identifier : AIDSLINE ICA10/94369433; Morlat P; Hubert JB; Chene G; Dequae L; Vimart E; Dabis F; Hopital Saint-Andre, INSERM U.330, Bordeaux, France.; OBJECTIVES: To compare the clinical and immunological features at time of AIDS diagnosis in patients who received Pneumocystis carinii (P.c.) prophylaxis (P+) and in those who did not (P-). METHODS: From 1985 to September 1993, data on 3117 HIV-infected patients have been prospectively recorded in a hospital-based sur
Natural history of HIV infection in infected Haitians with known date of seroconversion.: Int Conf AIDS. 1994 Aug 7-12;10(1):144 (abstract no. PB0002). Unique Identifier : AIDSLINE ICA10/94369434; Deschamps MM; Pape JW; Johnson WD Jr; OBJECTIVE: To determine the Natural history of HIV infection in seropositive heterosexuals with known date of seroconversion. METHODS: From 1987-1993, 19 individuals from 475 discordant couples seroconverted, based on CDC definition. Date of HIV seroconversion is midpoint between last HIV (-) and first HIV (+). They w
Natural history of HIV-infection in Bulgaria.: Int Conf AIDS. 1994 Aug 7-12;10(1):144 (abstract no. PB0003). Unique Identifier : AIDSLINE ICA10/94369435; Plochev KS; Dicov I; Duchovnicova T; Radinov A; Brankova J; Penkova K; Valcova I; Doganova I; M. M. A.-Centre of immunology, Sofia, Bulgaria.; OBJECTIVE: An analysis of the course of the HIV-infection in Bulgaria for the period 1986-93 was performed. METHODS: The patients were exposed to epidemiologecal, clinical, paraclinical and immunological analysis. The main methods used were flow cytometry, laser nephelometry, CAT
Acute and sustained immunosuppression with HIV seroconversion.: Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0006). Unique Identifier : AIDSLINE ICA10/94369436; Gupta KK; OBJECTIVE: Acute and sustained immunosupression with serious systematic opportunistic infection can occur immediately after HIV seroconversion. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. CMV Colitis was confirmed by biopsy, Immunoperoxidase stain and 10-fold in
Seronegativation in an asymptomatic HIV1 infected patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0008). Unique Identifier : AIDSLINE ICA10/94369437; Sousa A; Lourenco MH; Ferreira MO; Teles LC; Victorino RM; Faculdade de Medicina, Lisboa, Portugal.; OBJECTIVE: To report immunological and virological studies in an asymptomatic HIV infected patient in wham seronegativation was documented. METHODS: Two different ELISA methods were done as initial tests for HIV1 antibodies followed by Western blot to identify individual proteins sequentially done during the 6 years f
The slope of change in CD4 counts as prognostic factor in the progression of HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0005). Unique Identifier : AIDSLINE ICA10/94369438; Touloumi G; Hatzakis A; Karafoulidou A; Milona I; Kapsimali V; Economidou I; Mandalaki T; Dept. of Epidemiology, Athens University Medical School, Greece.; OBJECTIVE: To study the slope of decline in CD4 cell counts and to assess its relation to the risk of AIDS and age at seroconversion in a hemophilia cohort followed for 14 years. METHODS: 158 hemophiliacs who seroconverted to HIV between 1980 and 1985 were studied. The mean age (SD) at seroconversion was 26.2(15.4) wh
The surgical complications in AIDS: 92 cases.: Int Conf AIDS. 1994 Aug 7-12;10(1):145 (abstract no. PB0007). Unique Identifier : AIDSLINE ICA10/94369439; Lowenstein W; Arsac M; Hopital Laennec, Paris, France.; A prospective study, led in Laennec s hospital from 1985 to 1992, enabled us to observe 92 cases out of 1248 patients HIV+, about 7% (82 men, 10 women, average age 36.7). These patients were studied clinicaly by imaging procedure, biology and long-term follow up in order to set up the signification of these complicati
Clinical and pathological aspect of HIV-sero positive inpatients.: Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0012). Unique Identifier : AIDSLINE ICA10/94369440; Okubo T; Fujita H; Soga T; Mohri H; Nagashima Y; Misugi K; Ichikawa S; Soda K; 1st Dept. Int. Med., Yokohama City Univ. School of Medicine,; Japan.; OBJECTIVES: To describe the clinico-pathological aspect of HIV/AIDS inpatients. METHODS: We reviewed HIV/AIDS inpatients in our hospital since 1988. We examined risk factor, CD4 counts, CD4/8 ratio, cause of death and the survival time after diagnosis of AIDS. RESULTS: A total of 13 HIV sero-positive subjects were adm
Clinical progression in HIV1 and HIV2 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0010). Unique Identifier : AIDSLINE ICA10/94369441; Vasconcelos C; Castro-Melo J; Carvalho MF; Santos E; Cerveira C; Sarmento R; Marques R; Campos M; Servico de Imunologia, Hosp. Sto. Antonio, Porto, Portugal.; INTRODUCTION, POPULATION AND METHODS: A rate of clinical progression slower for HIV2 than for HIV1 has been generally reported but never substantiated. In the present study the clinical evolution, evaluated by Walter Reed staging (WR 1 to 6), was studied by actuarial curves in HIV1 (n = 50) and HIV2 (n = 36) patients
Envelope V2 and V3 regions in circulating HIV-1 from patients with SI-variants.: Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0009). Unique Identifier : AIDSLINE ICA10/94369442; Telenti A; Grunow R; Deiss V; Bernasconi C; Matter L; von Overbeck J; Univ. of Berne, Switzerland.; BACKGROUND: SI phenotype may result from selection of a minority species. To evaluate this possibility, we studied match serum and MT-2 culture supernatant. The V2 and V3 of gp120, known determinants of a SI phenotype, were sequenced to establish how accurately cultured SI isolates reflect the circulating species in v
The prevalence of slow progressors in HIV-1 infection in Galicia, Spain.: Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0011). Unique Identifier : AIDSLINE ICA10/94369443; Castro A; Hermida M; Pereiro C; Lopez Calvo MS; Ruanova S; Moreno J; Pedreira J; Internal Medicine Service, Hospital Juan Canolejo, La Coruna.; ++OBJECTIVES: To analyse the prevalence of patients with slow progressor criteria (SP) (patients infected > 5 years and CD4 counts > 500/mm3 with no antirretrovirical treatment) in HIV-1 in the La Coruna area of 500,000 inhabitants (Galicia, Northwestern Spain ). METHODS: 804 HIV-1 p
Gastrointestinal parasites and HIV-1 in Ugandan male adults.: Int Conf AIDS. 1994 Aug 7-12;10(1):147 (abstract no. PB0016). Unique Identifier : AIDSLINE ICA10/94369444; Mugyenyi P; Tugume S; Lutalo T; Kibende S; Joint Clinical Research Centre, Kampala, Uganda.; OBJECTIVE: To determine any association between gastro-intestinal tract parasites (GITPs) and HIV-1 serostatus among young Ugandan male adults. METHOD: Stool and blood samples of 1034 randomly selected males were examined for presence of GITPs and HIV-1 sero status respectively. Any association between particular GITP
HIV infection in women in Argentina five years observational study.: Int Conf AIDS. 1994 Aug 7-12;10(1):147 (abstract no. PB0013). Unique Identifier : AIDSLINE ICA10/94369445; Lupo S; Santarelli MT; Kneitschel R; Bortolozzi R; Navarret N; Taborda M; Hospital Centenario, University of Rosario, Argentina.; OBJECTIVE: to describe the epidemiology, clinical aspects and antiretroviral therapy in a group of HIV (+) in Rosario City, Argentina . MATERIAL: since 1988 until 9/93 a total of 87 HIV (+) women were followed in our institution. Median age was 26 range (15-55); 16% initial stage
Natural history of HIV-1 & HIV-2 in cohort of W. African females.: Int Conf AIDS. 1994 Aug 7-12;10(1):147 (abstract no. PB0015). Unique Identifier : AIDSLINE ICA10/94369446; Marlink R; Traore I; Thior I; Ndoye I; Essex M; Mboup S; Kanki P; Harvard AIDS Institute, Harvard School of Public Health,; Cambridge, MA 02138.; OBJECTIVE: A large cohort of female sex workers has been followed since 1985 in Dakar, Senegal , to determine the clinical and immunologic outcome of HIV-1 and HIV-2 disease. METHODS: Women enrolling in an outpatient clinic in Dakar were screened for HIV status after informed conse
Pathogeny recapitulates epidemogeny.: Int Conf AIDS. 1994 Aug 7-12;10(1):147 (abstract no. PB0014). Unique Identifier : AIDSLINE ICA10/94369447; Pieczenik G; Cook College, Rutgers University, New Brunswick, N.J. 08903.; I wish to propose that the course of the pathology of a disease in an individual, pathogeny, can recapitulate the fluctuations in virulence in a population during the course of an epidemic, epidemogeny. One model for the fluctuation in virulence in a population during the course of an epidemic is that of a non or less
Great disturbances of steroids excretion of AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):148 (abstract no. PB0020). Unique Identifier : AIDSLINE ICA10/94369448; Socolov IA; Pokrovsky VV; Emelianov BA; Semenov VA; Yurin OG; Gruzdev BM; Institute of epidemiology-AIDS center, Moscow, Russia.; METHOD: The amount of 15 different steroids is measured in urine samples of AIDS patients. Samples are compared before and after Chorionic Gonadotropin (ChG) stimulation test: (1500 iu--3 days-1500 iu--2 days + urinary sampling). RESULTS: The whole amount of urine steroids is 1/5 of the normal value. Decrease is promi
Do corticosteroids predispose to infections or Kaposi's sarcoma in HIV+ patients with low CD4 counts?: Int Conf AIDS. 1994 Aug 7-12;10(1):148 (abstract no. PB0019). Unique Identifier : AIDSLINE ICA10/94369449; Tetreault D; DeLeo M; Beck K; French C; Beall G; Harbor-UCLA Medical Center, Torrance.; OBJECTIVE: To determine if a causal relationship exists between systemic corticosteroid (CS) use (greater than 1 week) and the development of active cytomegalovirus disease ( CMV ), Kaposi s sarcoma (KS), and cryptococcosis (Cr
Is HIV disease progression influenced by CMV co-infection? SEROCO Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):148 (abstract no. PB0018). Unique Identifier : AIDSLINE ICA10/94369450; Salmon-Ceron D; Colasante U; Carre N; Deveau C; Persoz A; Rouzioux C; Bucquet D; Service de Medecine Interne, Hopital Cochin 27, Paris, France.; OBJECTIVE: To determine whether CMV seropositivity or CMV seroconversion accelerates HIV disease progression in a French cohort (SEROCO) of non-hemophiliac HIV-infected adult patients with known date of infection. METHODS: The date of HIV infection was the mid-p
Toxoplasmic myocarditis in an AIDS patient: diagnosis with an endomyocardial biopsy.: Int Conf AIDS. 1994 Aug 7-12;10(1):148 (abstract no. PB0017). Unique Identifier : AIDSLINE ICA10/94369451; Zucman D; Lebras P; Bicetre Hospital, France.; INTRODUCTION: Cardiac involvement is frequent although rarely symptomatic in AIDS patients. There are multiple causes of heart failure in this setting and when cardiac signs appear isolated it represents a difficult diagnostic problem. CASE RECORD: a 37-year-old homosexual man, diagnosed with HIV infection in 1986, is
Evidence of an in vitro association of HIV with other DNA viruses.: Int Conf AIDS. 1994 Aug 7-12;10(1):149 (abstract no. PB0023). Unique Identifier : AIDSLINE ICA10/94369452; Cisterna R; Campelo C; Gorrino MT; Malave C; Sarria L; Fernandez-Aranguiz A; Lardelli P; Sunen E; Madariaga L; Immunology, Microbiology and Parasitology Dept. University of the; Basque Country, Spain.; Several authors have focused their attention on the possibility that cofactors may be important in the pathogenesis of acquired immunodeficiency syndrome (AIDS). To investigate the association of Human Immunodeficiency Virus (HIV) with various DNA viruses e.g. Hepatitis B Virus (HBV), Citomegalovirus (
Herpesvirus antibodies levels in HIV2 positive population.: Int Conf AIDS. 1994 Aug 7-12;10(1):149 (abstract no. PB0024). Unique Identifier : AIDSLINE ICA10/94369453; Marques A; Palminha P; Graca A; National Institute of Health, Lisbon, Portugal.; The importance of Herpesvirus (Epstein-Barr Virus, Citomegalo Virus, Herpes Simplex Virus, Varicella zoster Virus) infections in the acquired immunodeficiency syndrome is not well established. A study of the serological profiles against Herpesvirus was carried out in HIV2 samples of patients attending an out-patient c
HIV-1 infection and liver disease in IDUs.: Int Conf AIDS. 1994 Aug 7-12;10(1):149 (abstract no. PB0021). Unique Identifier : AIDSLINE ICA10/94369454; Miguez-Burbano MJ; Lu Y; Page B; Gregory M; Fletcher MA; Quesada J; Shor-Posner G; Baum MK; Dept of Epidemiology, University of Miami School of Medicine, FL.; OBJECTIVE: As liver disease is frequently observed in intravenous drug users (IDUs), the clinical significance of liver dysfunction in HIV disease, and its relationship to immune activation, serum protein and lipid profiles, was determined in HIV-1 seropositive and seronegative IDUs. METHODS: Liver function tests (GGT
Trace elements in patients with HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):149 (abstract no. PB0022). Unique Identifier : AIDSLINE ICA10/94369455; Peraire J; Vidal F; Schuhmacher M; Domingo J; Porras J; Richart C; Department of Internal Medicine, Hospital Joan XXIII, Tarragona,; Spain.; OBJECTIVE: To provide a detailed characterization of trace elements concentrations in patients with HIV-1 infection. METHODS: Serum and urinary zinc, selenium, copper, iron, magnesium and manganese concentrations were determined in 19 patients AIDS (IVC1, CDC 1987) compared to 25 individuals with asymptomatic infectio
Behavioral intervention for Thai Army conscripts may reduce HIV incidence.: Int Conf AIDS. 1994 Aug 7-12;10(1):15 (abstract no. 032D). Unique Identifier : AIDSLINE ICA10/94369456; Bond K; Na Chiang MC; Vaddhanaphuti C; Eiumtrakul S; Nelson KE; Celentano DD; Johns Hopkins University, Baltimore, MD 21205.; OBJECTIVE: To estimate the impact of a behavioral intervention among conscripts in the Royal Thai Army in the Upper North on HIV incidence and risk reduction. METHODS: Four cohorts of 21 year old men conscripted by lottery were tested for HIV-1 between May 1991 to November 1993. A behavioral intervention focusing on r
Clients of female sex workers and unsafe sex.: Int Conf AIDS. 1994 Aug 7-12;10(1):15 (abstract no. 033D). Unique Identifier : AIDSLINE ICA10/94369457; Plumridge E; Chetwynd J; Dept Public Health, Christchurch School of Medicine, New Zealand.; OBJECTIVES: To study the contexts and circumstances leading to unsafe sex practices amongst clients of female sex workers in their commercial and non-commercial sexual encounters. METHODS: This was a 2-stage project involving both quantitative and qualitative research phases. Clients were contacted and interviewed in
Resistant to risk reduction: male clients of commercial sex workers in Kenya.: Int Conf AIDS. 1994 Aug 7-12;10(1):15 (abstract no. 035D). Unique Identifier : AIDSLINE ICA10/94369458; Ngugi EN; Njeru EK; Plummer FA; Moses S; Karanja M; University of Nairobi, Kenya.; INTRODUCTION: Sexual preference in relation to risk reduction should be explored so as to identify and support preventive behaviour while persuading those with unsafe sexual behaviour to modify them for control and prevention of STD/HIV. OBJECTIVE: To establish preferred sexual behaviour of men and facilitating factor
The gender gap: what young unmarried Latinos think & do about sex.: Int Conf AIDS. 1994 Aug 7-12;10(1):15 (abstract no. 034D). Unique Identifier : AIDSLINE ICA10/94369459; Marin BV; Gomez CA; Grinstead OA; Ctr. for AIDS Prevention Studies, University of California, San; Francisco.; BACKGROUND: Men and women may have different expectations and beliefs about sex because of Latino culture. We compare beliefs and behaviors of unmarried Latino men and women aged 18-24. METHOD: A cross sectional random digit dial telephone survey of 1600 unmarried Latino adults aged 18-49 in 10 U.S. states representin
Clinical and epidemiological profile of HIV1 and HIV2 infection in Dakar-Senegal.: Int Conf AIDS. 1994 Aug 7-12;10(1):150 (abstract no. PB0027). Unique Identifier : AIDSLINE ICA10/94369460; Diouf G; Ouangre A; Kanki P; Mboup S; Marlink R; Coll Seck A; Infectious Deseases Center (Fann), Boston.; OBJECTIVES: To determine the epidemiological differences between people living in an area where HIV1 and HIV2 exist. To determine the clinical profile of HIV1 and HIV2. To study natural history of HIV2. METHODS: From July 1991 to June 1993 a cross-sectional study was conducted including all patient admitted for his fi
CD8 hyperlymphocytosis in 17 HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):150 (abstract no. PB0025). Unique Identifier : AIDSLINE ICA10/94369461; Coutellier A; Autran B; Cherin P; Debre P; Herson S; Sce de Medecine Interne, G.H. Pitie Salpetriere, Paris,; France.; A CD8 hyperlymphocytosis is observed in 5 to 10% of HIV infected patients. It has been related to a diffuse visceral infiltration: The SICCA syndromee whose clinical observation can be compared to the Gougerot-Sjogren one. Its pronostic value is not clearly established. Seventeen HIV 1 seropositive patients (15 male a
HIV disease: clinical spectrum in state of Gujarat, India.: Int Conf AIDS. 1994 Aug 7-12;10(1):150 (abstract no. PB0028). Unique Identifier : AIDSLINE ICA10/94369462; Patel AK; Sheth JT; Vasa CV; Patel RK; Saxena DM; B.J. Medical College & Civil Hospital, Ahmedabad, Gujarat, India.; OBJECTIVE: Main objective of this study is to explore the clinical presentation of HIV disease in State of Gujarat, India . METHODS: The patients admitted to our institute with high risk behavior and clinical features related to HIV disease were subjected for HIV testing after counse
Trends in clinical entities: case definitions or real fact?: Int Conf AIDS. 1994 Aug 7-12;10(1):150 (abstract no. PB0026). Unique Identifier : AIDSLINE ICA10/94369463; Valdespino JL; Garcia ML; del Rio A; Salcedo A; Magis C; Mora JL; Sepulveda J; INDRE, SCD Secretariat of Health, Mexico.; OBJECTIVE: To determine trends of initial clinical entities in AIDS patients (1983 and 1993) and analyse impact of different definitions. METHODS: AIDS cases registry was analysed. RESULTS: Trends of clinical entities are as follows: TABULAR DATA, SEE ABSTRACT VOLUME. During 1983-84 the definition in use was that of C
Clinical features of patients with AIDS in Japan.: Int Conf AIDS. 1994 Aug 7-12;10(1):151 (abstract no. PB0031). Unique Identifier : AIDSLINE ICA10/94369464; Ajisawa A; Negishi M; Yamaguchi T; Tmagawa S; Department of Infectious Diseases, Tokyo Metropolitan Komagome; Hospital, Japan.; OBJECTS: A total of only 685 patients with acquired immunodeficiency syndrome (AIDS) had been reported in Japan as of the end of 1993. Since clinical reports of AIDS patients in Japan have been rare, we studied the clinical presentations of AIDS cases in Japan in an effort to ascert
HIV 1 and HIV 2 in commercial blood donors of Gujarat. An epidemiological study.: Int Conf AIDS. 1994 Aug 7-12;10(1):151 (abstract no. PB0029). Unique Identifier : AIDSLINE ICA10/94369465; Bhattacharya RD; Khatri F; GAP, SIRMCE, B/02, Siddha Chakra Apt. Ahmedabad, India.; OBJECTIVES: To evaluate HIV 1 and HIV 2 infection trend in commercial Blood Donors of Gujarat. METHODS: 4 times per year, blood samples were collected from CBDs. The project is running since last 4 years. Each sample has been collected with pre-test counselling. 535 forms on KAP study have been evaluated. RESULTS: Sin
The clinical presentation of HIV-1 disease and AIDS at the Chulalongkorn University Hospital in Bangkok, Thailand.: Int Conf AIDS. 1994 Aug 7-12;10(1):151 (abstract no. PB0032). Unique Identifier : AIDSLINE ICA10/94369466; Ruxrungtham K; Muller O; Teeratakulpisarn S; Sirivichakul S; Ubolyam S; Hanvanich M; Phanuphak P; Thai Red Cross Society, Bangkok, Thailand.; OBJECTIVE: To describe the clinical dimension of the Thai AIDS epidemic with respect to one hospital in Bangkok. METHODS: Retrospective analysis of data from 446 consecutive patients attending the AIDS outpatient clinic of the Chulalongkorn University Hospital in Bangkok during a 10 weeks period in 1993/94. RESULTS: O
The effect of AIDS epidemic on the death patterns in Mulago hospital, Uganda during 1993.: Int Conf AIDS. 1994 Aug 7-12;10(1):151 (abstract no. PB0030). Unique Identifier : AIDSLINE ICA10/94369467; Sezi CL; Mulago Hospital, Kampala, Uganda.; OBJECTIVES: To document, compare and contrast the disease entities causing death in 1973 pre-AIDS period and during the AIDS epidemic in 1993. METHODOLOGY: This was by retrospective analysis of the records from the death register for 1973 and 1993. Causes of death were analysed for the first 2000 cases in each year an
AIDS in Africa: clinical major problems and priorties for research.: Int Conf AIDS. 1994 Aug 7-12;10(1):152 (abstract no. PB0034). Unique Identifier : AIDSLINE ICA10/94369468; Kapita B; Bima M; Gen. Hospital, Kin.; OBJECTIVES: To identify the major clinical problems and their causes which lead to AIDS and death. To establish the priorties for clinical research. METHODS: Clinical prospective study of adult AIDS patients (WHO definition + ELISA) admitted to 2 hospitals of Kinshasa from january to june 1993. Recording of live-threa
HIV infection progression since first pulmonary tuberculosis episode (PTB).: Int Conf AIDS. 1994 Aug 7-12;10(1):152 (abstract no. PB0035). Unique Identifier : AIDSLINE ICA10/94369469; Mandala K; Mukadi YD; Ntikala B; Mayala M; Matela B; Nzila N; Projet SIDA, Kinshasa, Zaire.; OBJECTIVES: To determine the HIV attributable mortality and to characterize the immunologic evolution of PTB patients since the first episode onset. To quantify the PTB relapse rate after correct and specific treatment completion. METHODS: 365 HIV infected TB patients (+ smear sputum) and 203 TB patients were administ
HIV seroprevalence survey in a Guatemalan AIDS clinic.: Int Conf AIDS. 1994 Aug 7-12;10(1):152 (abstract no. PB0033). Unique Identifier : AIDSLINE ICA10/94369470; Arathoon EG; Estrada Y; Martin RM; Oroxom A; INTRODUCTION: The clinic run by the AGPCS offers testing, treatment and counseling for people with HIV/AIDS and is a model clinic in Guatemala for its innovative and integral follow up programs. RESULTS: From January to December 1993, at the AGPCS clinic, 107 new HIV (+) cases w
Profile of opportunistic infections among HIV-infected people in Hong Kong.: Int Conf AIDS. 1994 Aug 7-12;10(1):152 (abstract no. PB0036). Unique Identifier : AIDSLINE ICA10/94369471; Wong KH; Lee SS; Sitt WH; Li CK; Ho HF; Department of Medicine, Queen Elizabeth Hospital, Kowloon, Hong; Kong.; OBJECTIVE: To determine the spectrum of major and minor opportunistic infections in a cohort of patients with HIV infection in Hong Kong . METHOD: Case records of patients attending the HIV clinic--Special Medical Consultation Clinic
Characterization of AIDS patients surviving more than 5 years.: Int Conf AIDS. 1994 Aug 7-12;10(1):153 (abstract no. PB0038). Unique Identifier : AIDSLINE ICA10/94369472; Rozenbaum W; Maslo C; Jacomet C; Lebrette MG; Girard PM; Wirbel E; Inf. Dis. Unit, Hopital Rothschild, Paris, France.; OBJECTIVE: To describe the clinical and laboratory data in patients surviving more than 5 years after an AIDS diagnosis. METHODS: Retrospective review of computerized medical record of a cohort of AIDS patients followed from 1th June 1981 to 31th December 1988 by one unit. Kaplan Meier curve was used to estimate survi
Gender differences in infectious HIV viral burden among injecting drug users. ALIVE Study.: Int Conf AIDS. 1994 Aug 7-12;10(1):153 (abstract no. PB0040). Unique Identifier : AIDSLINE ICA10/94369473; Farzadegan H; Vlahov D; Nelson K; Johns Hopkins University, Baltimore, MD 21205.; OBJECTIVE: To compare infectious viral load among men and women IDUs. METHOD: A total of 285 IDUs, of whom 89 (31%) were women at various CD4 levels were studied. (Median CD4 = 394 range 2-2375). Infectious viral load was determined by cocultivation of serial 5-fold dilution of one million PBMC, with normal PBMC (IUPM
Mortality patterns in a hemophilia cohort followed for 14 years.: Int Conf AIDS. 1994 Aug 7-12;10(1):153 (abstract no. PB0039). Unique Identifier : AIDSLINE ICA10/94369474; Karafoulidou A; Touloumi G; Louizou C; Markakis C; Economidou I; Hatzakis A; Mandalaki T; Laikon Hospital, Athens, Greece.; OBJECTIVE: To describe the long term mortality patterns in a hemophilia cohort. METHODS: 158 hemophiliacs (149 hemophilia A, 4 hemophilia B and 5 Von Willebrand s disease) seroconverted to HIV between 1980 and 1985. The presumed date of seroconversion was calculated by smooth models based on spline functions (Kroner a
The HIV outpatient study (HOPS): early results.: Int Conf AIDS. 1994 Aug 7-12;10(1):153 (abstract no. PB0037). Unique Identifier : AIDSLINE ICA10/94369475; Marlowe S; Moorman A; Holmberg S; HOPS Investigators/Health Research Network, Atlanta, GA.; OBJECTIVE: To monitor trends in demographics, symptoms, diagnoses, and treatments of HIV-infected outpatients, especially early in infection. METHODS: We analyzed data electronically charted by trained clinic personnel on HIV-infected outpatients visits to clinician offices in the following US cities: Atlanta, GA, Tar
Clinical spectrum of HIV in Bombay.: Int Conf AIDS. 1994 Aug 7-12;10(1):154 (abstract no. PB0042). Unique Identifier : AIDSLINE ICA10/94369476; Maniar J; Hira S; GT Hospital, Bombay.; OBJECTIVES: To determine (1) clinical spectrum of HIV-1 &/or 2 infection (2) Positive Predictive Value (PPV) of clinical features. METHODS: Between July and December 1993, 533 patients referred to Skin/STD dept at GT Hospital in Bombay on basis of clinical suspicion of HIV-disease were entered into the study. Their
Laboratory markers of long-term survivors with HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):154 (abstract no. PB0041). Unique Identifier : AIDSLINE ICA10/94369477; Knight B; Salvato P; Thompson C; Houston Immuno. Institute, TX.; OBJECTIVE: To describe laboratory markers of HIV disease in long-term survivors. METHODS: 174 HIV-seropositive pts. were evaluated retrospectively by chart review over a 9-yr. period. 64 pts., HIV+ since 1985 and whose CD4 count remained > 500 after 9 years, were reviewed. Laboratory markers measured in the 9th year w
Longitudinal immunophenotypic studies on a cohort of asymptomatic HIV+ve patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):154 (abstract no. PB0043). Unique Identifier : AIDSLINE ICA10/94369478; Trickett A; Tesfamariam N; Dwyer J; Kwan YL; St. George Hospital, Sydney, Australia.; OBJECTIVE: To study the changing proportions of subsets of CD4 and CD8 cells over time in a cohort of asymptomatic HIV+ve patients. This work is being undertaken to aid in delineation of disease progression. METHODS: Blood samples obtained at yearly intervals from 21 patients were stained for 3 colour immunofluorescen
Urinary neopterin as a prognostic index of survival time in HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):154 (abstract no. PB0044). Unique Identifier : AIDSLINE ICA10/94369479; Rogstad KE; Morgan L; Hughes J; Bignell CJ; Department of Genitourinary Medicine, City Hospital, Nottingham,; UK.; OBJECTIVE: To assess the value of urinary neopterin as a predictor of survival time in patients infected with HIV-1. METHOD: This was a semi-longitudinal study of 67 patients (63 male, 4 female) seropositive for HIV-1 attending a genitourinary clinic and included those with homosexually and heterosexually acquired inf
alpha 1 Acid glycoprotein as early clinical marker reflecting pathophysiological state in SIV infected monkey.: Int Conf AIDS. 1994 Aug 7-12;10(1):155 (abstract no. PB0048). Unique Identifier : AIDSLINE ICA10/94369480; Mukai R; Matsui K; Murayama Y; Sata T; Kurata T; Fujii Y; Tamura K; Izumi M; Komine K; Takasaka M; et al; Tsukuba Primate Ctr. for Med. Sci. NIH, Japan.; OBJECTIVES: The survival time after SIV inoculation to rhesus monkeys varied from animal to animals. We searched for the parameters that could predict the fate of the monkeys inoculated with SIV. METHODS: SIVmac were inoculated to 8 rhesus and 2 cynomolgus monkeys. Six out of 8 rhesus macaque died of AIDS, while 2 cyn
CD4 lymphocyte depletion: correlation with P24 antigen, neopterin, beta 2 microglobulin, CD8 count.: Int Conf AIDS. 1994 Aug 7-12;10(1):155 (abstract no. PB0047). Unique Identifier : AIDSLINE ICA10/94369481; Salvato P; Thompson C; Houston Immunological Institute, Texas.; OBJECTIVE: To describe laboratory markers as related to CD4 cell decline in HIV disease progression. METHODS: 114 HIV+ pts. underwent lab. testing for CD4 count, P24 antigen, neopterin, beta 2 microglobulin and CD8 count. A chart review was conducted to determine length of known exposure based upon high-risk sexual ac
Prognostic factors in HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):155 (abstract no. PB0045). Unique Identifier : AIDSLINE ICA10/94369482; Cahn P; Santarelli MT; Ben G; Rolon M; Oshiro G; Perez H; Hospital Fernandez, Buenos Aires, Argentina.; OBJECTIVE: To identify significant prognostic factors influencing survival (SV) in a seroprevalent cohort. METHODS: Between 1/1984 and 5/1992, 328 HIV (+) pts. entered into this cohort. Features at diagnosis were: median age 27 years (range 16-63) gender: male (M) 256, female (F) 72; risk behavior: homosexuals (HS) 15
Prognostic value of HIV-1 biological phenotype for survival after AIDS diagnosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):155 (abstract no. PB0046). Unique Identifier : AIDSLINE ICA10/94369483; Koot M; van Leeuwen R; Danner S; Schuitemaker H; Tersmette M; Reis P; Central Lab. Blood Transf., Amsterdam, The Netherlands.; In about 50% of HIV-1 infected persons syncytium inducing (SI) HIV-1 variants emerge during the asymptomatic phase of infection. The presence of SI variants in asymptomatic men is predictive for the progression to AIDS, independent of CD4+ T-cell numbers. Here we studied survival among AIDS patients in relation to HIV
Cellular and serological activation markers including sICAM 1 in long-term observations.: Int Conf AIDS. 1994 Aug 7-12;10(1):156 (abstract no. PB0052). Unique Identifier : AIDSLINE ICA10/94369484; Schoenwald E; Duschet P; Marhold I; Gschnait F; Dermat. Depart., Hospital of Lainz, Vienna, Austria.; OBJECTIVE: Determination of the correlation of cellular and serological parameters including sICAM 1 by long term analysis from HIV positive non progressors versus progressors to AIDS. METHODS: 44 patients were studied at 512 visits (mean: 11), 15 patients developed AIDS within the observation period (1-73 months, mea
Opportunistic infections in patients with CD4 counts above 300.: Int Conf AIDS. 1994 Aug 7-12;10(1):156 (abstract no. PB0049). Unique Identifier : AIDSLINE ICA10/94369485; Stool E; Piot D; Gathe J Jr; Najjar A; Edwards S; Park Plaza Hospital, Special Diseases Unit, Houston, Texas.; OBJECTIVE: To detail the presentation of opportunistic infection (OI) in patients with CD4 counts > 300. METHODS: Retrospective review of five homosexual males, non-IV drug abusers who were followed by the authors prospectively. RESULTS: Patient 1: 33 yo male HIV positive for 64 months, on
Predictive value of symptomatic primary acute HIV-1 infection: a two years evaluation following contamination.: Int Conf AIDS. 1994 Aug 7-12;10(1):156 (abstract no. PB0051). Unique Identifier : AIDSLINE ICA10/94369486; Derancourt C; el M'Rini T; Borne MC; Drobacheff C; Vuitton D; Leroy J; Portier H; Laurent R; Department of Dermatology and Cellule Sida, University Hospital,; Besancon, France.; OBJECTIVE: To determine, two years following contamination, the predictive value of symptomatic primary HIV 1 infection (CDC classification, CD4 cells count) compared to asymptomatic seroconversion, in a retrospective study. METHODS: Two cohorts including 993 HIV 1 infected patients (1985-1994) were studied: Eleven pa
Soluble IL-2 receptor (sIL-2R) in seropositive individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):156 (abstract no. PB0050). Unique Identifier : AIDSLINE ICA10/94369487; Tateno Y; Takahashi T; Tateno M; Togashi T; Sato H; Yoshiki T; Dept. of Pediatrics, Hokkaido University, Sapporo, Japan.; OBJECTIVES: Elevated soluble IL-2 receptor (sIL-2R) levels have been reported in the sera from HIV-1 seropositive individuals. In this study, we determined plasma sIL-2R levels of seropositives every two to three months and investigated their relation to the clinical s
Correlation between apoptosis and lack of CD28 in HIV+ pts.: Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0056). Unique Identifier : AIDSLINE ICA10/94369488; Castelli F; Prati E; Brugnoni D; Malacarne F; Signorini L; Tebaldi A; Caligaris S; Cattaneo R; Carosi G; Clinic Infectious Diseases, Dpt Clinical Immunology, Univ.; Brescia, Italy.; OBJECTIVES: a) to identify the possible correlation between clinical features of HIV infection and spontaneous lymphocytes apoptosis level (SA), b) to verify if abnormal expression of molecules involved in T cell costimulation is a feature is a feature of cells undergoing AS. METHODS: nuclei of non-stimulated peripher
High levels of beta-hexoaminidase and A-mannosidase in sera of HIV+ individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0053). Unique Identifier : AIDSLINE ICA10/94369489; Lugering N; Stoll R; Kucharzik T; Busch H; Domschke W; Department of Medicine B, University Munster, Germany.; OBJECTIVE: 1. To assess the value of lysosomal glycosidases as markers for detection of the different stages of HIV disease. 2. To get information about mechanisms of release of lysosomal glycosidases under pathological conditions. METHODS: We measured activities of four different lysosomal glycosidases in sera of 62
Major lymphocyte values in seropositive west African prostitutes (HIV1 only, HIV2 only and HIV1&2) as compared to healthy seronegative west Africans.: Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0055). Unique Identifier : AIDSLINE ICA10/94369490; Hannet I; Strauss K; Siby T; Diaw RA; Ndaw M; Marlink R; Diouf G; M'Boup S; Becton Dickinson, Erembodegem, Belgium.; The percentage and absolute count values of T, B and NK lymphocytes as well as CD4 and CD 8 T lymphocytes in a cohort of HIV-infected prostitutes from Dakar were compared to values in healthy seronegative adult controls. TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION: CD4 T lymphocyte percentages and absolute counts di
Structural change in oligosaccharide chains of IgG in HIV-1 infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0054). Unique Identifier : AIDSLINE ICA10/94369491; Mizuochi T; Nakata M; Oka S; Shimada K; School of Engineering, Tokai University, Kanagawa, Japan.; OBJECTIVE: To determine if structures of oligosaccharide chains of IgG from HIV-1 infected individuals change according to the disease development. METHODS: IgG was purified from sera of hemophiliacs (n = 5) and HIV-1 infected hemophiliacs at CDC stage 2 (n = 5), 3 (n = 5), and 4 (n = 4) by ammonium sulfate precipitat
Analysis of immunological parameters showed that antiretroviral treatment is necessary for the PGL state.: Int Conf AIDS. 1994 Aug 7-12;10(1):158 (abstract no. PB0057). Unique Identifier : AIDSLINE ICA10/94369492; Takata N; Fujii T; Dept of Internal Med. Hiroshima Univ., Japan.; OBJECTIVE: We investigated the medical records and laboratory findings to find which parameters might be characteristic for the HIV disease progression. MATERIALS & METHODS: We compared the data of 30 patients with HIV infection (20 hemophiliacs and 10 pts with sexual contact; 28 males and 2 females; 36.4 +/- 10.9
Evaluation of immuno-status in HIV disease using serum 2'5'-oligoadenylate synthetase activity.: Int Conf AIDS. 1994 Aug 7-12;10(1):158 (abstract no. PB0060). Unique Identifier : AIDSLINE ICA10/94369493; Ueda M; Higasa S; Suehiro A; Kakishita E; 2nd Dept. of Internal Medicine, Hyogo College of Medicine, Japan.; OBJECTIVE: 2 5 -oligoadenylate synthetase (2-5AS) has been reported to be induced by interferons (IFN) and its activity in mononuclear cells reliably reflects activation of the IFN system by viral infections. To evaluated the immuno-status in HIV disease and the progression of the disease, serum 2-5AS activities in he
HIV DNA in seropositive subjects showing stable levels of CD4 counts.: Int Conf AIDS. 1994 Aug 7-12;10(1):158 (abstract no. PB0059). Unique Identifier : AIDSLINE ICA10/94369494; Garbuglia AR; Salvi R; Di Caro A; Benedetto A; Montella A; Recchia O; Di Sora F; Lauria F; Delfini C; Centre of Virology, S. Camillo Hosp., Rome, Italy.; Since 1982 the progression to AIDS of about 1,000 HIV seropositive individuals was evaluated by investigating several clinical parameters and in particular the CD4 lymphocyte counts decline. We observed nine asymptomatic subjects showing stable or increased levels of CD4 counts, even if seven years or more elapsed fro
Immune activation markers and CD4 cells counts in HIV clinical stages. Predictors of advanced disease?: Int Conf AIDS. 1994 Aug 7-12;10(1):158 (abstract no. PB0058). Unique Identifier : AIDSLINE ICA10/94369495; Portilla J; Sanchez J; Boix V; Munoz C; Sen ML; Reus S; Perez-Mateo M; Hospital General de Alicante, Spain.; OBJECTIVE: To evaluate the relationship among serum neopterin, beta 2 microglobulin, IgG, IgA, IgM, clinical stage and CD4 lymphocyte counts. METHODS: In 108 HIV patients (IVDU 80%), 89 men and 19 women, we determined CD4 cells by flow cytometry; beta 2 microglobulin, IgG, IgA e IgM by immunonefelometry and serum neop
Biological markers in the study of HIV infected and HIV non-infected drug abusers.: Int Conf AIDS. 1994 Aug 7-12;10(1):159 (abstract no. PB0061). Unique Identifier : AIDSLINE ICA10/94369496; Miro JM; Bedini JL; Serrano J; Filella X; Mallolas J; Lozano L; Hospital Clinic i Provincial, Barcelona, Spain.; PURPOSE--To stablish reference values for neopterin (NP), beta 2 microglobulin (B2M), TNF and soluble interleukin 2 receptor (IL-2R) for both HIV infected assymptomatic drug abusers (HIV DA) and HIV non infected drug abusers (Non-HIV DA). METHODS--97 Non-HIV DA and 164 HIV DA were studied. HIV DA were followed periodi
Otolaryngological findings in HIV infections.: Int Conf AIDS. 1994 Aug 7-12;10(1):159 (abstract no. PB0064). Unique Identifier : AIDSLINE ICA10/94369497; Goncalves D; Silva MS; Toledo Junior AC; Greco DB; Immunodeficiency sector, UFMG, Brazil.; OBJECTIVE: Report the frequency and types of otolaryngological (ENT) manifestations in various stages of HIV infections. METHODS: HIV infected patients with ENT symptoms were examined. They were seen by one specialist that didn t know about the stage of HIV infection. RESULTS: The majority were male (50 = 87.7%), betw
Relations of trace element status to immunological activity markers and progression of human immunodeficiency virus infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):159 (abstract no. PB0063). Unique Identifier : AIDSLINE ICA10/94369498; Dousset B; May T; Dubois F; Allavena C; Rabaud C; Nabet-Belleville F; Canton P; Department of Biochemistry and Infectious Diseases, CHU Nancy,; France.; OBJECTIVE: To analyze the relationships between blood trace elements and beta 2 microglobuline levels in the 2 years follow-up of HIV infected patients with respect to the clinical evolution. METHODS: The study group consisted of 148 patients (36 females and 112 males, age: 33 +/- 9 years) at various stages of HIV inf
Thyroid function during the HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):159 (abstract no. PB0062). Unique Identifier : AIDSLINE ICA10/94369499; Garcia Quintana A; Capdevila JA; Ocana I; Barbera J; Castellanos JM; Catalan R; Galarza R; Hospital General Vall d'Hebron, Barcelona, Spain.; OBJECTIVE: To evaluated the thiroidal abnormalities that appeared during HIV infection. METHODS: Measurement of thiroid hormones (T3, T4, T4L, TSH) by ELISA in 86 patients (67 male) stratified in three groups according to their CD4 cells count. RESULTS: Group I (CD4 > 500): Fourteen males and 8 females. Mean hormonal
HIV incidence in adults in northern Thailand.: Int Conf AIDS. 1994 Aug 7-12;10(1):16 (abstract no. 038C). Unique Identifier : AIDSLINE ICA10/94369500; Khamboonruang C; Beyrer C; Natpatan C; Eiumtrakol S; Celentano D; Nelson KE; Research Institute for Health Sciences, Chiang Mai University,; Thailand.; OBJECTIVE: To measure the HIV-1 incidence, followup rates and high risk behaviors in cohorts of seronegative adults in northern Thailand . METHODS: We screened cohorts of female commercial sex workers (CSWs), male STD clinic pts, royal Thai Army (RTA) conscripts, and discharged RT
HIV infection in India--trends analysis.: Int Conf AIDS. 1994 Aug 7-12;10(1):16 (abstract no. 039C). Unique Identifier : AIDSLINE ICA10/94369501; Lal S; Khodakevich L; Salil P; National AIDS Control Organization, New Delhi, India.; OBJECTIVE: To review the trends of the spread of HIV infection in India . METHODS: Analysis of the data recorded by 62 surveillance centres set up in all 32 States and Union Territories of India, by area and in time. RESULTS: As of 31 Jan 1994 out of about 2 million samples screened
Male intervention programme in red light area of Bombay.: Int Conf AIDS. 1994 Aug 7-12;10(1):16 (abstract no. 036D). Unique Identifier : AIDSLINE ICA10/94369502; Thanekar JG; Nigudkar P; Municipal Corporation of Greater Bombay, India.; OBJECTIVE: To increase knowledge about HIV/AIDS and STDs among clients of prostitutes. METHOD: Approached potential clients of prostitutes through an organized street campaign consisting of street theatre, one person shows, corner meetings and poster exhibitions. Humour is used in all activities. When possible perform
The epidemiology of HIV/AIDS in the Western Pacific Region: an opportunity for prevention.: Int Conf AIDS. 1994 Aug 7-12;10(1):16 (abstract no. 037C). Unique Identifier : AIDSLINE ICA10/94369503; Sarda R; Petersen G; Gertig D; Effler P; WHO Regional Office for the Western Pacific.; OBJECTIVE: To develop a concise overview of the epidemiology of HIV/AIDS in the Western Pacific Region. METHODS: Examination of official surveillance reports and reviews of serosurveys conducted at sentinel sites within Member States. RESULTS: Collection of epidemiologic data on HIV and AIDS has been ongoing in the We
A semi-automated 4-color method for T-cell subset enumeration.: Int Conf AIDS. 1994 Aug 7-12;10(1):160 (abstract no. PB0068). Unique Identifier : AIDSLINE ICA10/94369504; Mandy F; Bergeron M; Shenkin M; Horton A; Mylvaganan R; Rud E; LCDC, HPB, Health Canada, Ottawa, ON.; Precise, rapid and cost effective determination of T-cell subsets for initiation and maintenance of HIV+ patient therapy is a continual challenge for clinical immunologists. Guidelines released by US agencies, such as AIDS Clinical Trial Group (ACTG) of NIH, and the Center for Disease Control and Prevention, both reco
Lymphoproliferative response correlates to the markers of HIV-infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):160 (abstract no. PB0065). Unique Identifier : AIDSLINE ICA10/94369505; Markova R; Beshkov D; Argirova R; Center of Immunology, Military Medical Academy, Sofia, Bulgaria.; OBJECTIVE: To look for a possible correlation between lymphoproliferative response to phytohaemagglutinin (PHA) and recombinant interleukin (rIL-2) and the known markers of HIV-infection. METHODS: Ten HIV-infected persons (7 with AIDS and 3 asymptomatic) were followed-up in 3-6 month intervals. Lymphoproliferative res
Progression of HIV infection from the moment of probable transmission.: Int Conf AIDS. 1994 Aug 7-12;10(1):160 (abstract no. PB0066). Unique Identifier : AIDSLINE ICA10/94369506; Meira DA; Souza LR; Marcondes-Machado J; Zacchi B; Faculdade de Medicina de Botucatu, UNESP, Brazil.; OBJECTIVE: In Brazil little is known about the progression of AIDS infection considered from the moment of transmission. The objective of the study was to observe this situation. METHODS: 65 HIV infected individuals with probable moment of contagion determined were studied, after a
Variant HIV RNA distribution within lymph nodes and tonsils of stage matched patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):160 (abstract no. PB0067). Unique Identifier : AIDSLINE ICA10/94369507; Turnicky R; Anderson D; Nau M; Zhao F; Coats J; Mayers D; Division of Retrovirology, Walter Reed Army Institute of; Research, Rockville, MD 20850.; OBJECTIVE: We used human lymphoid tissue as a model to better define early HIV staging and possibly monitor response to therapies. METHODS: 5 Lymph node and 3 tonsil biopsies were obtained from stage II matched patients, and 1 non-HIV control. Tissues were formalin fixed, and snap frozen in liquid nitrogen. Immunopero
Dysregulation of cytokine production as new surrogate marker in HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):161 (abstract no. PB0069). Unique Identifier : AIDSLINE ICA10/94369508; Tateyama M; Fukutake K; Hattori T; Ohmoto Y; Dept. of Clin. Pathol., Tokyo Medical College, Japan.; OBJECTIVE: We have evaluated whether cytokine productions were improved after initiation of zidovudine (ZDV) or dideoxyinosine(ddI) therapy and correlated with clinical outcome. PATIENTS: A total of 72 HIV-1 positive hemophiliacs (25 patients presented with CDC stage A1, 30 with stage A2 and 17 with C3, AIDS) were enr
A community-based study of the reliability of absolute CD4 lymphocyte counts in HIV infected subjects.: Int Conf AIDS. 1994 Aug 7-12;10(1):161 (abstract no. PB0070). Unique Identifier : AIDSLINE ICA10/94369509; Jimmerson C; Bockmon K; Harrison J; Houston Clinical Research Network, TX 77006.; OBJECTIVES: CD4 cell counts are commonly used as surrogate markers. High variability within these markers could limit their usefulness as a tool for making treatment decisions. The variability of CD4 counts and percentages performed by commercial laboratories was investigated. METHODS: Three nationwide, contract labor
ALT as a marker for hepatitis B and C in drug users.: Int Conf AIDS. 1994 Aug 7-12;10(1):161 (abstract no. PB0072). Unique Identifier : AIDSLINE ICA10/94369510; Brooks K; Orr S; Fenaughty A; Fisher D; Center for Alcohol & Addiction Studies, University of Alaska,; Anchorage.; OBJECTIVE: To assess the usefulness of alanine transaminase (ALT) as a surrogate marker for detecting hepatitis C and hepatitis B in the sera of intravenous drug users (IVDUs) and crack cocaine smokers. METHOD: Data were collected from the Risk Behavior Assessment (RBA), a structured interview, which was administered
CD4+/CD8+ subset immunoassay in HIV+ adults and children.: Int Conf AIDS. 1994 Aug 7-12;10(1):161 (abstract no. PB0071). Unique Identifier : AIDSLINE ICA10/94369511; Denny T; Jensen B; Garcia A; Vella F; Gavin E; Stein D; Oleske J; Wong W; UMDNJ, Newark.; OBJECTIVE: The purpose of this study was to compare the absolute CD4+ and CD8+ lymphocyte levels obtained by the predicate flow cytometry/hematology method with those resulting from a new alternative fluorescent immunoassay (Zymmune CD4/CD8 Cell Monitoring Kit, Zynaxis, Inc., Malvern, PA) in both adult and pediatric s
Anti-p24 titer: a marker for AIDS in Cuban HIV seropositives.: Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0076). Unique Identifier : AIDSLINE ICA10/94369512; Benitez J; Rivero J; CIGB, Havana, Cuba.; OBJECTIVE: To evaluate the use of anti-p24 antibody titer as a marker for the prognosis of AIDS in Cuban HIV-1 seropositives. METHODS: An ELISA for anti-p24 titration, based on recombinant HIV-1 p24, which uses a protein-A-peroxidase conjugate was used. Serum samples were 1:2 serially diluted from 1:20 to 1:2560. Both
Course of CD8+CD38+ lymphocyte subset during continuous AZT-ddC combination therapy in 25 HIV infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0073). Unique Identifier : AIDSLINE ICA10/94369513; Holmgren C; Knechten H; Praxiszentrum, Aachen, Germany.; INTRODUCTION: The CD8+CD38+ lymphocyte subset is a marker for progression of HIV infection but there have been few reports about the course of this subset during antiretroviral combination therapy . Thus, development of this subset may indicate response to therapy or pr
Inter laboratory assessment of the FACSCount System.: Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0074). Unique Identifier : AIDSLINE ICA10/94369514; Shiba A; Landay A; Ullery S; Webster K; Becton Dickinson Immunocytometry Systems, San Jose, CA.; The standard methodology for enumeration of T lymphocyte subsets requires an absolute lymphocyte count from a hematology analyzer in addition to lymphocyte subset percentages determined by flow cytometry. A simple, precise, and inexpensive option to standard methodology would allow greater access to quality absolute C
Monitoring of CD8/DR lymphocyte subset in pediatric HIV infection: a preliminary study.: Int Conf AIDS. 1994 Aug 7-12;10(1):162 (abstract no. PB0075). Unique Identifier : AIDSLINE ICA10/94369515; Tzantzoglou S; Ajassa C; Berardelli G; Falciano M; Bellagamba R; Catania S; Infect. Dis. La Sapienza Univ., Rome, Italy.; OBJECTIVE: to value the presence of quantitative changes of CD8/DR in different stages of pediatric HIV infection and their significance as a predictive marker of disease progression. METHODS: we followed the course of CD4, CD8, CD8/DR, beta 2M, HIV-Ag in 6 children with HIV infection by vertical trasmission (mean age
CD5+/CD8+ percentage ratio: a new evolution predictive marker of HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):163 (abstract no. PB0078). Unique Identifier : AIDSLINE ICA10/94369516; Traina C; D'Alia G; Barone T; Centro AIDS, USL 58-Palermo, Italy.; With reference to a study about the ratio CD5+/CD8+ as evolution marker during HIV infection (Valguarnera e coll.), we studied 90 subjects HIV infected in differents stages of disease (25 asymptomatics patients, 30 LAS, 20 ARC, 15 AIDS) and compared them with the homogeneous group of healthy HIV negative subjects. The
Heterosexual transmission of HIV in Rio de Janeiro: immunological aspects.: Int Conf AIDS. 1994 Aug 7-12;10(1):163 (abstract no. PB0077). Unique Identifier : AIDSLINE ICA10/94369517; Morgado M; Guimaraes MD; Neves Junior I; Castilho E; Department of Immunology (IOC-FIOCRUZ), Rio de Janeiro, Brasil.; OBJECTIVE: To evaluate immunological aspects among female partners (FP) of HIV infected male index cases (IC) in Rio de Janeiro, Brazil . METHODS: ICs and their FPs were recruited from participant centers in Rio de Janeiro, Brazil. They should be sexually active within the past yearw
Immunological markers indicating the AIDS evolution.: Int Conf AIDS. 1994 Aug 7-12;10(1):163 (abstract no. PB0079). Unique Identifier : AIDSLINE ICA10/94369518; Jauregui Rueda H; Balbaryski J; del Castillo M; Monticelli A; L.I.C. Laboratory Buenos Aires, Argentina.; OBJECTIVE: 1) To evaluate four immunological markers in HIV infected patients classificated according to the CDC classification, April 1992. 2) To determine if some of the studied markers be of more predictive value in the progression of the infection. 3) The relationship between markers and mortality. METHODS: The rew
Use of CD4 to predict outcome in HIV emergencies.: Int Conf AIDS. 1994 Aug 7-12;10(1):163 (abstract no. PB0080). Unique Identifier : AIDSLINE ICA10/94369519; Chang R; Memorial Hospital, New York, NY 10021.; The CD4 count has been used as a marker for progression of HIV and incorporated into staging and severity of illness scores for HIV, but its usefulness as a prognostic marker in an acute setting has not been explored. We investigated the use of CD4 in predicting hospitalization in acutely ill HIV patients. A series ofw
Adenosinedeaminase (ADA): indicator of evolution of the HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0081). Unique Identifier : AIDSLINE ICA10/94369520; Monticelll A; Balbaryski J; Lewi D; Jauregui Rueda H; COINSIDA Fundation, Buenos Aires, Argentina.; OBJECTIVE: 1) To determine the use of the ADA test as an indicator of evolution of the HIV infection. 2) To evaluate the correlation between the ADA test and the CD4+ linphocytes count values. 3) Put forward values for the ADA test equivalent to CD4+ ones in the CDC classification. METHODS: One hundred and ten HIV posw
Association of proviral load and immune disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0083). Unique Identifier : AIDSLINE ICA10/94369521; Conway B; Ko DS; Dufour D; Salas T; Montpetit M; U. of Ottawa AIDS Research Group and Bureau of HIV/AIDS; Laboratories & Research, Ontario, Canada.; OBJECTIVE: To evaluate the relationship between circulating proviral load and the severity of immune disease. METHODS & RESULTS: Mononuclear cells (MCs) were isolated from 129 HIV-infected individuals and proviral load (copies/10(6) CD4 cells) measured by quantitative PCR. CD4 cell counts, in discrete categories, w
Precision and stability of CD4, 8 and 3 values in HIV+ and HIV-populations in Shirati Tanzania as evaluated by FACSCount.: Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0084). Unique Identifier : AIDSLINE ICA10/94369522; Strauss K; Brubaker G; Kumbi D; Shao J; Krenkler W; Blattner W; Levin A; Becton Dickinson, Europe, Belgium.; The performance characteristics of a dedicated CD4 counting system, FACSCount, were evaluated in populations from Shirati, Tanzania . Precision assessments involved 4 replicate stainings and analyses per subject. The pooled CV s of the parameters evaluated (CD4,8,3 and 4:8 ratio)
Reducing the variation in CD4 lymphocyte count measurements.: Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0082). Unique Identifier : AIDSLINE ICA10/94369523; Raboud JM; Haley L; Montaner JS; Murphy C; Januszewska M; Schechter MT; Univ of British Columbia, Vancouver.; OBJECTIVE: To quantify the variation in CD4 counts of HIV infected individuals due to laboratory and physiological factors and to find strategies to reduce variation. METHODS: Blood was drawn from 30 HIV +ve clinically stable men 3 times in each of 2 weeks, 4 weeks apart. At each visit, 2 tubes of blood were drawn. Du
Activated CD8-cells--a new surrogate marker?: Int Conf AIDS. 1994 Aug 7-12;10(1):165 (abstract no. PB0087). Unique Identifier : AIDSLINE ICA10/94369524; Hoffmann UJ; Mischo M; Reichelt D; Busch H; Zidek W; Medizinische Poliklinik, University of Munster, Germany.; OBJECTIVE: To determine the usefullness of CD38+CD8+ and HLA-DR+CD8+ cells for monitoring of antiretroviral therapy. METHODS: HIV infected patients who started or changed an antiretroviral therapy were controlled for lymphocyte subsets, beta 2-microglobulin, p24 antigen and antibodies, and immunoglobulins at baseline
Delayed type hypersensitivity skin testing in HIV-infected persons, Chiangrai, Thailand.: Int Conf AIDS. 1994 Aug 7-12;10(1):165 (abstract no. PB0086). Unique Identifier : AIDSLINE ICA10/94369525; Yanai H; Uthaivoravit W; Sawanpanyalert P; Limpakarnjanarat K; Mastro TD; Chiangrai Health Club, Muang Chiangrai, Thailand.; OBJECTIVE: To examine the utility of delayed type hypersensitivity (DTH) skin tests in HIV-infected individuals in Chiangrai, northern Thailand . METHODS: We examined the reactivity to three DTH skin test antigens (PPD, Mumps, and Candida) in 201 asymptomatic HIV-infected individu
Progressive biological markers of HIV infection depending on different risk groups and CDC stages.: Int Conf AIDS. 1994 Aug 7-12;10(1):165 (abstract no. PB0088). Unique Identifier : AIDSLINE ICA10/94369526; Fuster M; Martinez C; Cortes M; Cadafalch J; Domingo P; Barrio J; Sambeat MA; Internal Medicine Department, Programa SANT PAUCITRAN, Hospital; de Sant Pau, Barcelona, Spain.; OBJECTIVE: To study the biological parameters of HIV progressive infection depending on different risk groups. METHOD: Different biological parameters (B2-M, neopterine, CD4 linfocites, triglicerides, IgA and ADA) have been studied in a sample of 524 HIV+ patients. From the first blood extraction the patients were div
Skin tests as an immunosuppression marker in HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):165 (abstract no. PB0085). Unique Identifier : AIDSLINE ICA10/94369527; Viana L; Pardini R; Toledo Junior AC; Jeronymo M; Astete F; Greco DB; Antunes CM; UFMG, Belo Horizonte, Brazil.; OBJECTIVE: to evaluate the skin tests as an immunosuppression marker in HIV infection in relation to absolute CD4+ T-lymphocytes (CD4+) count and clinical presentation. METHODS: eighty-six HIV infected individuals classified according to CDC criteria (1993) were evaluated retrospectively for absolute CD4+ count (manua
Cryoglobulinemia in HIV infected patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0092). Unique Identifier : AIDSLINE ICA10/94369528; Redeker S; Schmit JL; Fuentes V; Prin L; Infectious Disease Unit, University Hospital Amiens.; The aim of this study was to assess cryoglobulinemia in HIV infected patients. Fifty seropositive patients were studied. Eleven patients were IVDU, 20 homosexual males, 16 heterosexual individuals, 4 bisexuals, 4 blood recepients and 7 patients coming from an endemic zone. Mean delay from first HIV seropositivity was
Heat versus acid dissociation of immune complexes for the detection of P24 antigenemia.: Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0089). Unique Identifier : AIDSLINE ICA10/94369529; Schechter M; Oliveira E; Pinto ME; Souza VM; Bronstein M; AIDS Program, Federal University of Rio de Janeiro, Bronstein; Lab., Brazil.; INTRODUCTION: P24 antigenemia is widely used as a surrogate marker of disease progression and of therapeutic efficacy. Immune complex dissociation has been shown to greatly increase the sensitivity of p24 detection assays and has become the standard technique. OBJECTIVE: To compare heat (HD) versus acid (AD) dissociat
Natural killer (NK) cells in HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0091). Unique Identifier : AIDSLINE ICA10/94369530; Whitelaw J; Brettle R; Scott G; Robertson R; OBJECTIVE: The aim of the study was to look at the concentration of Natural Killer (NK) cells in two risk groups infected with HIV 50 intra-venous drug users were analysed and 50 homo/bisexual men were the second cohort. METHOD: Blood was incubated with dual labelled monoclonal antibodies as follows- 1. lymphocyte gat
Two-color flow cytometric determination of absolute counts of lymphocyte subpopulations in HIV-infected patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):166 (abstract no. PB0090). Unique Identifier : AIDSLINE ICA10/94369531; Goto T; Takano K; Kimura S; Shionogi Biomed. Lab., Univ. of Tokyo, Japan.; OBJECTIVE: To find out good surrogate markers for analysis of pathophysiology and prediction of prognosis of HIV disease. SUBJECTS AND METHODS: Absolute counts of lymphocyte subpopulations (CD4 x CD45RA, CD4 x CD29, CD8 x CD11b, CD16 x CD56) were analyzed by two-color flowcytometry using Cytron-Absolute (Ortho) in 74
p24 antibody level as a prognostic marker in HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0096). Unique Identifier : AIDSLINE ICA10/94369532; Ghioni G; Fernandez E; Viglianco R; Bortolozzi R; Taborda M; Fay O; Center for technology in Public Health, National University of; Rosario Argentina.; OBJECTIVE: To evaluate the pronostic usefulness of p24 Ab cuantitation in HIV infection. MATERIAL AND METHODS: 61 patients HIV positive were included. Anti p24 from Boehringer Mannheim was ran. We used: Elisa test from Organon Teknika and Abbott Laboratories ; confirmatory test fro
Comparison of surrogate markers predictive for rapid progression of HIV disease in patients with initial CD4 counts of 200 to 500.: Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0094). Unique Identifier : AIDSLINE ICA10/94369533; Lange M; Klein EB; Inada Y; Maitra US; Mohan VP; St. Luke's-Roosevelt Hosp. Ctr., Columbia University, NY, NY; 10025.; OBJECTIVE: HIV positive patients with CD4 counts 200 to 500 are at increased risk for AIDS. The optimal time to begin antiretroviral therapy remains controversial. A reliable method to sort out rapid progressors versus slow progressors could permit a more individualized therapeutic approach. We previously reported on
Serum neopterin concentration in HIV infected IVDUs.: Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0093). Unique Identifier : AIDSLINE ICA10/94369534; Rogowska-Szadkowska D; Borzuchowska A; Prokopowicz D; Grzeszczuk A; Chodynicka B; Andrzejewicz A; Rogowski F; Dep. Infect. Dis., Medical Sch., Bialystok, PL.; OBJECTIVE: Analysis of changes in the serum concentration of neopterin (NPT) in HIV infected IVDUs in relation to current using or not using intravenous drugs. METHODS: NPT concentrations were measured using radioimmunoassay (IMMUtest Neopterin-Henning Berlin) in the serum of 34 HIV positive asymptomatic IVDUs. Twenty
Viral phenotype and clinical outcome in HIV+ children.: Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0095). Unique Identifier : AIDSLINE ICA10/94369535; Munoz-Fernandez MA; Navarro J; Obregon E; Garcia D; Gurbindo D; Sampelayo T; Fernandez-Cruz E; Dept. Immunology, H. Gregorio Maranon, Madrid, Spain.; OBJECTIVE: To investigate whether highly replicative HIV-1 isolates in children has the ability to induce syncytium formation (SI) in PBMC and MT-2 cells, whereas slow/low viruses lack this capacity. METHODS: Sixty-four infants born of seropositive HIV mother were studied. Virus isolation was performed by cocultivatio
Comparison of HIV viral load markers during therapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):168 (abstract no. PB0099). Unique Identifier : AIDSLINE ICA10/94369536; Donovan R; Bush C; Manzor O; Golembiesky A; Baxa D; Markowitz N; Saravolatz L; Henry Ford Hospital, Detroit, MI 48202.; OBJECTIVE: This study compared the changes in viral load measured by two plasma markers (HIV RNA and HIV p24 antigen) and one cellular marker (HIV unintegrated DNA, uDNA) during nucleoside therapy. METHODS: Peripheral blood was obtained from 19 HIV-infected patients (median CD4 count = 153/microliters) just prior to s
Correlation of selected CD8 lymphocyte activation markers with quantitative cellular viremia in asymptomatic HIV infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):168 (abstract no. PB0100). Unique Identifier : AIDSLINE ICA10/94369537; Levacher-Clergeot M; Bouscarat F; Dazza MC; Sinet M; Strauss K; Girard PM; Rozenbaum W; Coulaud JP; Pocidalo JJ; INSERM U13, Paris, France.; OBJECTIVE: Multi-colour flow cytometry has demonstrated significant alterations within the CD8 subsets co-expressing activation markers in HIV infection. Our study focuses on the correlation of these CD8 subsets with viral load in asymptomatic HIV infected patients with no marked CD4 lymphocyte depletion. PATIENTS AND
Flow cytometric analysis of peripheral blood lymphocyte subsets in healthy Chinese adults in Hong Kong.: Int Conf AIDS. 1994 Aug 7-12;10(1):168 (abstract no. PB0097). Unique Identifier : AIDSLINE ICA10/94369538; Kam KM; Lee SS; Mak WP; Lee SH; Department of Health, Yaumatei Polyclinic, Kowloon.; OBJECTIVE: To define by flow cytometry reference ranges on the absolute no. and relative percentage of total T-lymphocyte (CD3+), T-helper (CD4+), T-suppressor (CD8+) in healthy Chinese. METHOD: A FACScan flow cytometer, SimulSET software, and conjugate monoclonal reagents (Simultest IMKPlus) were used to analyse 207
Is neopterin a diagnostic marker in early HIV neuromanifestations?: Int Conf AIDS. 1994 Aug 7-12;10(1):168 (abstract no. PB0098). Unique Identifier : AIDSLINE ICA10/94369539; Jandl M; Enzensberger W; Enzensberger R; Proescholdt M; Demisch L; Fischer PA; Dept. of Neurology, University Hosp. Frankfurt/Germany.; OBJECTIVE: Diagnosis of underlying HIV infection may be difficult in early neuromanifestations. In this study we tried to assess the role of neopterin as a further tool for detecting HIV infection. METHODS: Follow-up examinations in CSF and serum were performed in a female patient with acute meningoencephalitis before
Prognostic value of CD4+ count and CD8+ T-cell activation in HIV seropositive patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):169 (abstract no. PB0101). Unique Identifier : AIDSLINE ICA10/94369540; Declercq E; Lavreys L; Kestens L; Vanham G; Colebunders R; Gigase P; Institute of Hygiene and Epidemiology, Brussels, Belgium.; OBJECTIVE: To study the value of CD4+ T-lymphocytes and of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ cells in predicting the risk to develop clinical AIDS. METHODS: The selected population comes from a set of 753 HIV seropositive patients for whom CD4+ count and expression of CD3
Serologic and viral correlates of HIV-1 disease progression; a multicenter AIDS cohort report. MACS Study. Multicenter AIDS Cohort Study.: Int Conf AIDS. 1994 Aug 7-12;10(1):169 (abstract no. PB0102). Unique Identifier : AIDSLINE ICA10/94369541; Black C; Farzadegan H; Henrard D; Saah A; Renaldo C; Detel R; Phair J; Johns Hopkins University, Baltimore, MD 21205.; OBJECTIVE: To compare HIV viral RNA and P24 antigen as well as hemoral response to the infection among seroconverters (SC) who developed AIDS in less than 3 years with SC who did not develop AIDS. Seroprevalents (SP) with no CD4 decline in 7 years of follow up were also used as control. METHOD: 17 SCs who developed AI
Ultrastructure of nuclear bodies in Kaposi's sarcoma associated AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):169 (abstract no. PB0103). Unique Identifier : AIDSLINE ICA10/94369542; Oyafuso LK; Hayashi H; Hotta Y; Inst. Infect. Emilio Ribas, S. Paulo, Brazil.; OBJECTIVES: To evaluate the morphological and ultra-structure alteration of the cells composing the SK lesion. Nuclear bodies could be seen in spindle-cell and undifferentiated endothelial cell as intranuclear structures. METHODS: Biopsies from AIDS associated Kaposi s sarcoma were obtained from 11 patients not previo
Vesnarinone inhibits AIDS-KS cells in culture.: Int Conf AIDS. 1994 Aug 7-12;10(1):169 (abstract no. PB0104). Unique Identifier : AIDSLINE ICA10/94369543; Petit RG; Miles S; Magpantay L; Mitsuyasu R; Otsuka America Pharmaceutical, Rockville, MD.; OBJECTIVE: Vesnarinone, OPC8212, is an inhibitor of TNF and IL-6 production in some cell culture systems. Our group has previously shown that these factors act as mitogens for AIDS-KS in culture. We wanted to determine if vesnarinone inhibits AIDS-KS cells in culture. METHODS: Cultured AIDS-KS cells were plated at 2,0
Epidemiological pattern of HIV infection in Yunnan Province, China.: Int Conf AIDS. 1994 Aug 7-12;10(1):17 (abstract no. 042C). Unique Identifier : AIDSLINE ICA10/94369544; Cheng H; Zhang JP; Zhao SD; Kou JD; Jia HH; AIDS Control Department, Yunnan Provincal Health & Anti-epidemic; Center, Kunming, China.; BACKGROUND: Yunnan Province is located in south western China . Its border with Vietnam , Laos and Myanmar is over 4000 Km
Human immunodeficiency virus (HIV) epidemiological trends among risk groups in Korea.: Int Conf AIDS. 1994 Aug 7-12;10(1):17 (abstract no. 041C). Unique Identifier : AIDSLINE ICA10/94369545; Shin YO; Hur SJ; Kim SS; Kee MK; Shin Center for AIDS Research, NIH, Seoul, Korea.; OBJECTIVE: To monitor and estimate the prevalence of HIV infections particularly among sexually risk groups in Korea with year. METHODS: HIV antibody tests had been performed on possible HIV risk groups in Korea during the period of 1985 to January 1994. Target populations of the surveillance were homosexual men, spou
HIV infection in Malaysia: cases seen at the University Hospital, Kuala Lumpur.: Int Conf AIDS. 1994 Aug 7-12;10(1):17 (abstract no. 040C). Unique Identifier : AIDSLINE ICA10/94369546; Ismail R; Dept. Medicine, University of Malaya, 59100 Kuala Lumpur.; The introduction of HIV virus in to Malaysia was estimated to be around the early 1980 s. The first case of AIDS was reported here in 1986. As of December 1993, the numbers have increased to 7400 HIV positive individuals detected in the country. The majority of them were detected
Lymphomagenesis in SIV immunosuppressed monkeys.: Int Conf AIDS. 1994 Aug 7-12;10(1):17 (abstract no. 043B). Unique Identifier : AIDSLINE ICA10/94369547; Biberfeld P; Li SL; Kaaya EE; Feichtinger H; Rezikyan S; Putkonen P; Immunopathology Lab., Karolinska Institute, Stockholm, Sweden.; OBJECTIVE: To study lymphomagenesis associated with SIV/HIV-2 infection in cynomolgus monkeys (Macaca fascicularis). METHODS: Monkeys were infected with SIVsm or HIV-2 and were studied for clinical signs of immunodeficiency and consecutive biopsies examined for B-cell clones and association with herpes virus, by immun
Kaposi's sarcoma: a lymphoscintigraphic study of HIV-infected and HIV-seronegative patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):170 (abstract no. PB0108). Unique Identifier : AIDSLINE ICA10/94369548; Cestari S da C; Petri V; Castiglioni ML; Alchorne MM; Katiki T; Escola Paulista de Medicina, Sao Paulo, Brazil.; OBJECTIVE: to evaluate extension of lymphatic involvement/damage in cases of Kaposi s sarcoma (KS) of HIV-infected and non HIV-infected individuals. A lymphologic perspective is focused to discuss correlation between KS genesis and lymphatic disorder(s). METHODS: Lymphoscintigraphy (radiolabeled 99mTc-Dextran intrader
Epidemiologic approaches: AIDS related Kaposi's sarcoma (KS).: Int Conf AIDS. 1994 Aug 7-12;10(1):170 (abstract no. PB0106). Unique Identifier : AIDSLINE ICA10/94369549; Haverkos HW; Drotman DP; NIH, Rockville, MD 20857.; OBJECTIVE: We reviewed 12 epidemiologic studies conducted among gay men with AIDS to examine the role of potential cofactors in the development of KS. METHODS: Aspects of the studies reviewed include basic study design, wording of the questionnaires (regarding nitrite inhalant use provided by investigators), and publi
Kaposi's sarcoma (KS) epidemiology in a public hospital district, Houston, TX.: Int Conf AIDS. 1994 Aug 7-12;10(1):170 (abstract no. PB0105). Unique Identifier : AIDSLINE ICA10/94369550; Shandera WX; Wilson J; Ben Taub Hospital, Baylor College of Medicine, Houston, TX 77030.; OBJECTIVE: To assess KS epidemiology among patients treated in Harris County Hospital District. METHODS: A retrospective review of demographic and clinical variables, among all AIDS patients with KS, treated between 1987 and 1994, identified by coding and surveillance data. RESULTS: Of 286 possible cases, 225 records
On the epidemiologic association between endemic Kaposi's sarcoma and malaria.: Int Conf AIDS. 1994 Aug 7-12;10(1):170 (abstract no. PB0107). Unique Identifier : AIDSLINE ICA10/94369551; Baumann S; Geier SA; Noehl MA; Goebel FD; Medizinische Poliklinik, LMU, Munich, Germany.; OBJECTIVE: Kaposi s sarcoma (KS) is a frequent tumor in subsaharan Africa (= endemic KS), and in patients with the acquired immunodeficiency syndrome (= AIDS-associated KS). Endemic KS has been attributed to environmental factors. The purpose of this study was to look for an association between endemic KS and malaria.
AIDS-associated Kaposi sarcoma and incidence of gastrointestinal involvement--how efficient is endoscopy.: Int Conf AIDS. 1994 Aug 7-12;10(1):171 (abstract no. PB0112). Unique Identifier : AIDSLINE ICA10/94369552; Liess H; Zoller WG; Bogner J; Powitz F; Zietz C; Goebel FD; Medizinische Poliklinik, University of Munich, Germany.; OBJECTIVE: Gastrointestinal involvement of AIDS-associated Kaposi Sarcoma (KS) may be of major prognostic as well as therapeutic relevance. Therefore the incidence of gastrointestinal involvement was retrospectively analysed using data from autopsies performed in an unselected group of patients (pts.) with AIDS. METHO
Improvement in diagnosis of visceral Kaposi-sarcoma by endoscopic ultrasound (EUS).: Int Conf AIDS. 1994 Aug 7-12;10(1):171 (abstract no. PB0111). Unique Identifier : AIDSLINE ICA10/94369553; Goebel FD; Bogner JR; Liess H; Zoller WG; Medizinische Poliklinik, University of Munich, Germany.; OBJECTIVE: Almost 50% of the patients with AIDS and Kaposi Sarcoma (KS) as the most frequently occurring tumor show visceral involvement. Gastrointestinal KS usually is diagnosed using endoscopy. To evaluate the use of Endoscopic Ultrasound as a complementary method scans were performed to assess informations about di
Presentation of pulmonary Kaposi sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):171 (abstract no. PB0110). Unique Identifier : AIDSLINE ICA10/94369554; Huang L; Schnapp LM; Goodman PC; Hopewell PC; Stansell JD; San Francisco General Hospital, University of California, 94110.; OBJECTIVE: Describe the clinical characteristics and presentation of pulmonary Kaposi sarcoma (KS). METHODS: Retrospective chart review of pulmonary KS cases diagnosed by bronchoscopy at San Francisco General Hospital from 1985-1993. RESULTS: During the 9 year review period, 200 cases of pulmonary KS were diagnosed at
Protein-losing enteropathy developing in an AIDS patient with duodenal Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):171 (abstract no. PB0109). Unique Identifier : AIDSLINE ICA10/94369555; Takechi A; Masuda G; Ajisawa A; Negishi M; Yamaguchi T; Yamada Y; Fujii H; Tokyo Metropolitan Komagome Hospital.; A Japanese homosexual, HIV-positive man aged in his 30s was admitted to Tokyo Metropolitan Komagome Hospital in the fall of 1992. He had been presenting with Kaposi s sarcoma (KS) persisting over his body surface a year before the admission. His general condition was good. Blood data included: WBCs 7,000/microliters;
Characteristics of Kaposi's sarcoma in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):172 (abstract no. PB0116). Unique Identifier : AIDSLINE ICA10/94369556; Stavrianeas N; Botsi C; Polydorou D; Zagoreos J; Chatzakis A; Stratigos I; Unit of specific infectious diseases, A. Sygros Hospital, Athens,; Greece.; OBJECTIVES: Localization of Kaposi s sarcomas in AIDS patients, their stage (CDC classification) and immunological status at the time of diagnosis. METHODS: 89 patients with AIDS-Kaposi (out of 246 AIDS patients), hospitalized in the A. Sygros Hospital during the 1988-1993 period, were studied. Eighty seven of our pat
Kaposi's sarcoma associated with AIDS. A comparison of clinical manifestations between intravenous drugs users (IVDU) and homosexuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):172 (abstract no. PB0114). Unique Identifier : AIDSLINE ICA10/94369557; Carriedo C; Rubio R; Pulido F; Moreno S; Buzon L; Pintado V; Barros C; Valencia E; Verdejo J; Costa JR; et al; Hospital 12 de Octubre, Unidad de Infeccion HIV, Madrid, Spain.; OBJECTIVES: To evaluate clinical differences between IVDU and homosexual patients with Kaposi s Sarcoma (KS) associated with AIDS. METHODS AND PATIENTS: We review the hospital records of 170 patients with KS associated with AIDS diagnosed in Madrid from 1984 to April 1992. 32 patients (18.8%) were IVDU male and 138 pa
Kaposi's sarcoma associated with AIDS. A comparison of clinical manifestations between male and female.: Int Conf AIDS. 1994 Aug 7-12;10(1):172 (abstract no. PB0115). Unique Identifier : AIDSLINE ICA10/94369558; Carriedo C; Rubio R; Pulido F; Moreno S; Buzon L; Pintado V; Barros C; Valencia E; Verdejo J; Costa JR; et al; Hospital 12 de Octubre, Unidad de Infeccion HIV, Madrid, Spain.; OBJECTIVES: To compare the differential features of Kaposi s Sarcoma (KS) associated with AIDS between male and female. METHODS AND PATIENTS: We review 200 patients with KS associated with AIDS diagnosed in Madrid from 1984 to April 1992. 189 patients (94.5%) were male and 11 patients (4.5%) were female. Up to April 1
Serum hyaluronan in HIV and in AIDS related Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):172 (abstract no. PB0113). Unique Identifier : AIDSLINE ICA10/94369559; Blum L; Guechot J; Picard O; Cabane J; Giboudeau J; Imbert JC; Intern Med Dept, St Antoine Hosp, Paris, France.; OBJECTIVES: Hyaluronan (hyaluronic acid, HA) is synthesized by mesenchymal cells, activated by growth factors and cytokines (EGF, PDGF, TGF beta, IGF-1, IL1). Kaposi s sarcoma (KS) is a tumor of mesenchymal origin, and data suggest that the induction of the lesions involves a pathway of events mediated by cytokines. W
A phase II study of oral etoposide (VP-16) in AIDS-related Kaposi's sarcoma (KS).: Int Conf AIDS. 1994 Aug 7-12;10(1):173 (abstract no. PB0118). Unique Identifier : AIDSLINE ICA10/94369560; Mans D; Sprinz E; Sander I; Kalakun F; Jung G; Prolla G; Schwartsmann G; South American Office for Anticancer Drug Development, Brasil.; OBJECTIVE: To access the toxicity and antitumor activity of oral VP-16 monotherapy in relation to plasma pharmacokinetics in patients with advanced AIDS-related KS. POPULATION & METHODS: Sixteen homo/bisexual male patients with AIDS-related KS NYU stage HIA-IVB were enrolled in the study. The mean age was 36 years
Effect of interferon alpha 2A on serum androgen in HIV+ men with Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):173 (abstract no. PB0120). Unique Identifier : AIDSLINE ICA10/94369561; Christeff N; Gharakhanian S; Thobie N; Wirbel E; Rozenbaum W; Nunez EA; U224 INSERM, CHU Bichat, Paris.; OBJECTIVE: To compare the serum androgen concentrations of HIV+ men with Kaposi s sarcoma (KS), before, during and after treatment with recombinant alpha-2a interferon (INF) (Hoffman-La Roche). We have detected high serum androgen levels in HIV+ KS men. METHODS: Serum from 22 HIV+ men with KS were analysed for androge
Liposomal doxorubicin in AIDS-related Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):173 (abstract no. PB0119). Unique Identifier : AIDSLINE ICA10/94369562; Caldeira L; Antunes F; Carvalho C; Duarte N; Doroana M; Forte M; Servico de Doencas Infecciosas, Hospital de Santa Maria,; Lisbon.; The authors report their experience with a new formulation of liposomal doxorubicin (DOX-SL) in the treatment of this disease. Seven HIV1+ male homosexual pts of caucasian origin (ages 29-42) were treated i.v. with 20mg/m2/cycle of this investigational formulation. At the beginning of therapy all pts had KS-associated
Reasons for significant disruptions in the planned therapy of AIDS-related Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):173 (abstract no. PB0117). Unique Identifier : AIDSLINE ICA10/94369563; Toi M; Caven G; Gilden J; Myers AM; UCHSC-Denver General Hospital.; OBJECTIVE: To determine the frequency and causes for any significant disruption in planned treatment for AIDS-related Kaposi s Sarcoma (KS). METHODS: The care of the last 50 consecutive patients treated for AIDS-related KS from 1990 through 1993 was reviewed. A significant disruption in therapy was defined to be any d
A randomized trial of 2 doses of alpha interferon (IFN) added to AZT for the treatment of Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):174 (abstract no. PB0122). Unique Identifier : AIDSLINE ICA10/94369564; Beaulieu R; Shepherd F; Murphy K; Gelmon K; Thuot C; Sawka C; Singer J; Canadian HIV Trials Network.; OBJECTIVES: Moderate to high-dose IFN induces clinical response in up to 40% of pts with KS, but, pts require frequent dose reduction or discontinuation of treatment for toxicity, and high dose IFN may not be given concurrently with AZT . This study wa
An alternative choice to stage (and treat) the epidemic Kaposi's sarcoma (EKS).: Int Conf AIDS. 1994 Aug 7-12;10(1):174 (abstract no. PB0121). Unique Identifier : AIDSLINE ICA10/94369565; Moresco L; Scala M; Meszaros P; Badellino F; Clinical and Exp. Onc. Inst., University of Genoa, Italy.; In order to classify the EKS according to the TNM programs, and to propose an alternative treatment for EKS patients (pts.), from June 1991 to Sept. 1993, two study-groups of pts. were investigated. In the first group 314 pts. were staged according to the--Tumor, Immune system, Sistemic illness (TIS) classification (c
Efficacy and safety of Stealth liposomal doxorubicin (DOX-SL) in AIDS-related Kaposi's sarcoma. The International Dox-SL Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):174 (abstract no. PB0123). Unique Identifier : AIDSLINE ICA10/94369566; Goebel FD; Medizinische Poliklinik, Universitat Munchen, Germany.; OBJECTIVE: The utility of current chemotherapeutic regimens in the treatment of AIDS-related Kaposi s sarcoma (AIDS-KS) is often compromised by both limited efficacy and substantial toxicity. Dox-SL, a long-circulating, liposomally encapsulated doxorubicin, has been demonstrated to specifically deliver high concentrat
Response to sublingual alfa interferon in Kaposi's sarcoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):174 (abstract no. PB0124). Unique Identifier : AIDSLINE ICA10/94369567; Lanjewar DN; Shetty CR; Shroff HJ; Hira SK; Grant Medical College, Bombay, India.; OBJECTIVE: To determine 1) therapeutic response of sublingual alfa interferon, 2) the cause of death in case of AIDS-associated Kaposis sarcoma. METHODS: An autopsy study was carried out on a 35 year old female who presented with large papulonodular lesions on extremities for which she was treated with sublingual alfa
EBV type A and B association with primary CNS lymphomas.: Int Conf AIDS. 1994 Aug 7-12;10(1):175 (abstract no. PB0125). Unique Identifier : AIDSLINE ICA10/94369568; Ciardi M; Del Re V; Toma L; Fedele CG; Cirelli A; Sorice F; Dept. Infectious Diseases, La Sapienza University, Rome, Italy.; OBJECTIVE: Epstein-Barr virus (EBV) is often associated with HIV infection and seems to play a pathogenic role on AIDS-related primary lymphomas of the central nervous system (CNS); in fact EBV virus has been detected in both brain tissue by in situ hybriditation and cerebrospinal fluid (CSF) by polymerase chain react
Hodgkin's disease and HIV infection (HD-HIV) in 114 patients (pts). GICAT.: Int Conf AIDS. 1994 Aug 7-12;10(1):175 (abstract no. PB0126). Unique Identifier : AIDSLINE ICA10/94369569; Errante D; Serraino D; Franceschi S; Nasti G; Bernardi D; Boiocchi M; Carbone A; Tirelli U; Rossi G; Ridolfo A; Division of Medical Oncology and AIDS, Centro di Riferimento; Oncologico, Aviano, Italy.; OBJECTIVE: To evaluate the epidemiological, virological, pathological and clinical features of HD-HIV in 144 pts of the GICAT. METHODS: Since November 1986, 114 cases (103 m, 11 f) of HD-HIV have been collected by the GICAT. A comparison with 125 Italian HD pts not infected with HIV, observed in the same period of tim
Multiple coin lesions revealing a primary pulmonary lymphoma in a patient with AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):175 (abstract no. PB0128). Unique Identifier : AIDSLINE ICA10/94369570; Allegre T; Cailleres S; Tadrist Z; Leprince Y; Jarry JM; Sault MC; Blanc AP; Hopital Cezanne, Aix-en-provence, France.; Pulmonary lymphoma is rarely reported in AIDS patients. A 28 year old HIV infected male was admitted to the hospital with dyspnea, cough, and fever. He was intravenous drug user and his past history was remarkable for pneumocystis carini pneumonia; The CD4 positive cell count was 16/mm3.X-ray film of the chest showed
Under-reporting of AIDS-associated Kaposi's sarcoma (KS) and non-Hodgkin's lymphomas (NHL).: Int Conf AIDS. 1994 Aug 7-12;10(1):175 (abstract no. PB0127). Unique Identifier : AIDSLINE ICA10/94369571; Serraino D; Franceschi S; Dal Maso L; Rezza G; Vaccher E; Tirelli U; Division of Epidemiology, Aviano Cancer Centre, Italy.; OBJECTIVE: Since AIDS surveillance data tend to underestimate the prevalence of AIDS-associated KS and NHL, this study was aimed at quantifing the degree of under-reporting of such neoplasms in the Italian AIDS surveillance scheme (RAIDS). METHODS: A linkage study between the Italian Cooperative Group on AIDS-related
HIV-related non Hodgkin lymphomas (NHL): report of 53 cases.: Int Conf AIDS. 1994 Aug 7-12;10(1):176 (abstract no. PB0129). Unique Identifier : AIDSLINE ICA10/94369572; Schlacht I; Landonio G; Nosari AM; Caggese L; Orso M; Schiantarelli C; Dept. of Infectious Diseases, Niguarda Hospital, Milan, Italy.; OBJECTIVE: to evaluate retrospectively 53 cases of HIV-related NHL, followed in our Department in the period 1986-1993. ANALYSIS OF CASES: 43 were males, 10 females. Median age was 30 yrs (r. 26-54). CD4+ were
Oral non-Hodgkin's lymphoma (ONHL) in 7 HIV-positive homosexual males.: Int Conf AIDS. 1994 Aug 7-12;10(1):176 (abstract no. PB0131). Unique Identifier : AIDSLINE ICA10/94369573; Lozada-Nur F; Silverman S; Univ. of Calif., S.F.; Non-Hodgkin s lymphoma (NHL) has emerged as a major sequela of HIV infection. As patient life expectancy increases due to early intervention with multiple drug therapies for HIV infection, there has been a significant increase in the occurrence of NHL and ONHL. Recognition is difficult since ONHL can appear similar to
Two cases of primary intracavitary cardiac lymphoma in immunocompromised patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):176 (abstract no. PB0130). Unique Identifier : AIDSLINE ICA10/94369574; Trylesinski A; Troisvallets D; Ghanem M; Godard S; Nizou R; Service de Medecine Interne, Centre Hospitalier, Gonesse,; France.; Primary cardiac lymphoma is rare with frequency at autopsy of 0.001-6%. Sixty cases were reported before 1985 and 11 cases in AIDS in the past 3 years. Intracavitary masses are the least commun: 5 to 9%; we report 2 cases. A 30 years old African man presented fever, chest pain and weight loss. Inflammatory pattern wit
407 patients (pts) with AIDS-related non-Hodgkin's lymphoma (AIDS-NHL): the experience of the GICAT (Italian Cooperative Group on AIDS and Tumors).: Int Conf AIDS. 1994 Aug 7-12;10(1):176 (abstract no. PB0132). Unique Identifier : AIDSLINE ICA10/94369575; Tirelli U; Spina M; Vaccher E; Nasti G; Bernardi D; Serraino D; Rizzardini G; Fasan M; Division of Medical Oncology and AIDS, Centro di Riferimento; Oncologico, Aviano, Italy.; OBJECTIVE: To analyze the outcome of 407 pts with AIDS-NHL. METHODS: Since november 1986, we have collected data on 407 pts with peripheral AIDS-NHL observed by the GICAT. At the Aviano Cancer Center, in the same period of time 93 pts have been treated according to prospective protocols. Based on HIV-related prognosti
Are some HIV-related non-Hodgkin's lymphomas curable malignancies?: Int Conf AIDS. 1994 Aug 7-12;10(1):177 (abstract no. PB0136). Unique Identifier : AIDSLINE ICA10/94369576; Vaccher E; Tirelli U; Errante D; Serraino D; Tavio M; Spina M; Bernardi D; Nasti G; Carbone A; Division of Medical Oncology and AIDS, C.R.O., Aviano, Italy.; OBJECTIVE: The overall outcome of patients with HIV-related non-Hodgkin s lymphomas (HIV-NHL) is poor because of the adverse clinico-pathological features and of the underlying HIV infection. However, the increased experience of physicians using intensive chemotherapy, has led to an improvement in the prognosis of sys
Clinical regression and lack of progression non-Hodgkin's lymphoma (Burkitt type) during treatment with ganciclovir (GCV) in a HIV (+) patient with cytomegalovirus retinitis.: Int Conf AIDS. 1994 Aug 7-12;10(1):177 (abstract no. PB0135). Unique Identifier : AIDSLINE ICA10/94369577; Luna N; Bistoni A; David DO; Rawson Hospital, Cordoba, Argentina.; OBJECTIVE: To report a case of clinical regression and lack of progression on a Non-Hodgkin s Lymphoma (Burkitt type) during treatment with Ganciclovir (GCV) in a HIV (+) patients with cytomegalovirus retinitis. CASE REPORT: A 35 years old patient, HIV(+), stage C3 (CDC), with a fa
Proton magnetic resonance in vivo spectroscopic energy metabolite screening in AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):177 (abstract no. PB0134). Unique Identifier : AIDSLINE ICA10/94369578; Sharma R; Sharma A; Agrawala A; Rehani MM; I.I.T. Delhi, India.; An attempt was made to monitor the effect of cisplatin i.v. therapy in AIDS related lymphoma. Before therapy and after treatment, NMR spectra were compared. Confirmed lymphoma with AIDS carriers (n = 8) were screened by proton MR H-1 spectra for PCr, ATP, Pi, PDE and PDM peaks. Results show the energy loss in tissue r
Survival impact of prior opportunistic infection (OI) of Kaposi's sarcoma (KS) among AIDS related lymphomas (ARL).: Int Conf AIDS. 1994 Aug 7-12;10(1):177 (abstract no. PB0133). Unique Identifier : AIDSLINE ICA10/94369579; Schmilovich A; Perez H; Gil Deza E; Marantz A; Savignano R; Cahn P; Acad. Mac. de Medicina, Buenos Aires, Argentina.; Many prognostic factors had been studied in ARL with controversial results but uniformly previous OI and/or KS are useful to indentify a group of patients (pts) with poor survival (S). Between 3/87 and 6/93, 31 pts with ARL were treated with mono or polichemotherapy at three single institutions in
AIDS-related primary central system lymphoma treated with radiotherapy: over a decade of clinical experience at UCSF.: Int Conf AIDS. 1994 Aug 7-12;10(1):178 (abstract no. PB0137). Unique Identifier : AIDSLINE ICA10/94369580; Ling C; Coleman L; Roach M; Wara W; OBJECTIVES: To determine the length of survival of patients with AIDS-related primary central nervous system lymphoma (PCNSL) after treatment with radiotherapy, and to determine whether length of survival (LOS) correlated with dose of radiotherapy, pre-treatment Karnofsky performance status (KPS), risk factors, age, C
A case of HIV positive hemophilia a with hepatocellular carcinoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):178 (abstract no. PB0140). Unique Identifier : AIDSLINE ICA10/94369581; Tao M; Karino Y; Yoshida J; Kobayashi S; Hashino S; Imamura M; Sakurada K; Miyazaki T; 3rd Dept. of Int. Med., Hokkaido Univ. Sch. Med., Sapporo, Japan.; OBJECTIVE: Double infection with HIV and HCV is commonly observed in hemophilia patients. Nevertheles, there are a few reports on the development of hepatocellular carcinoma (HCC) in these patients. We analyzed correlation between HIV infection and the development of HCC due to HCV infection. RESULTS: HCC developed in
Chemotherapy and didanosine (DDI) in AIDS-lymphoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):178 (abstract no. PB0138). Unique Identifier : AIDSLINE ICA10/94369582; Harrington WJ; Ucar A; Cabral L; Lai S; Byrnes JJ; Univ. of Miami, FL.; OBJECTIVE: To explore the hypothesis that combined antiretroviral and antitumor chemotherapy is feasible and more effective. METHOD: A randomized clinical trial was undertaken of stage II-IV intermediate or high grade lymphoma. 16 pts were randomized to the DDI (+) arm and 14 to the DDI (-) arm. Chemotherapy was deliv
Signet cell carcinoma of the stomach in a patient with AIDS: a case report.: Int Conf AIDS. 1994 Aug 7-12;10(1):178 (abstract no. PB0139). Unique Identifier : AIDSLINE ICA10/94369583; Uchida T; Takamatsu Red Cross Hospital, Japan.; OBJECTIVE: A case of signet cell cancer associated with AIDS is presented. CASE HISTORY: A 50-year-old Japanese man with hemophilia A had required multiple injections of factor VIII concentrate since 1973. He developed dysphagia, heartburn and hiccups on July 1987. He lost 10kg in weight during 2 months. No lymph-node
Increasing incidence of HIV-related lymphomas, 1983-1992.: Int Conf AIDS. 1994 Aug 7-12;10(1):179 (abstract no. PB0142). Unique Identifier : AIDSLINE ICA10/94369584; Beck EJ; Peters BS; Desmond N; Stuart S; Tomlinson D; Harris J; Weber JN; Academic Department of Public Health, St Mary's Hospital Medical; School, London, UK.; AIM: to study the incidence of HIV-related lymphomas and survival from time of diagnosis of lymphoma. METHODS: retrospective case-notes analysis of 50 patients with Non Hodgkin Lymphomas (NHL) 1/1/ 83-31/12/ 92. Analyses involved rank correlations, logistic regression and survival analyses. RESULTS: TABULAR DATA, SEE
Interferon alpha-2a+zidovudine vs. bleomycin+zidovudine for HIV-associated Kaposi's sarcoma. The Swiss Group for Clinical Studies on AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):179 (abstract no. PB0141). Unique Identifier : AIDSLINE ICA10/94369585; Opravil M; Hodapp K; Luthy R; Div. of Infectious Diseases, University Hospital, Zurich,; Switzerland.; OBJECTIVE: To compare the efficacy and tolerability of two combination treatments for HIV-associated Kaposi s sarcoma (KS) in a prospective randomized multicenter study. METHODS: Patient with progressive cutaneous KS were randomized to receive zidovudine (2 x 250 mg/d) with either interferon alpha-2a (IFN: 9 Mio U/d)
Testicular germ-cell tumors (GCT) in 23 patients (PTS) with HIV infection. GICAT (Italian Cooperative Group on AIDS and Tumors).: Int Conf AIDS. 1994 Aug 7-12;10(1):179 (abstract no. PB0143). Unique Identifier : AIDSLINE ICA10/94369586; Bernardi D; Errante D; Vaccher E; Tumolo S; Spina M; Nasti G; Marini B; Repetto L; Pizzoccaro G; Monfardini S; et al; Centro di Riferimento Oncologico, Aviano, Italy.; Between November 1986 and January 1994, 23 cases of GCT were observed within the GICAT. Thirteen pts had seminoma (SGCT) and 10 non seminoma (NSGCT). Sixteen were IVDUs, 3 homosexuals, 2 IVDU+homosexual, 1 hemophiliac and 1 with unknown risk factor. At diagnosis 1 (4.3%) patient had AIDS, 5 (21.7%) pts ARC, 4 (17.4%)
The Canadian randomized open-label trial of combination therapy for MAC bacteremia: mortality results. The Canadian MAC Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):179 (abstract no. PB0144). Unique Identifier : AIDSLINE ICA10/94369587; Zarowny D; Cameron W; Fong I; Salit I; Lemieux C; Raboud J; Shafran S; Canadian HIV Trials Network, Vancouver.; OBJECTIVE: A randomized open label trial begun in November, 1992 is comparing the efficacy and safety of ciprofloxacin 750 mg BID, ethambutol 15 mg/kg QD, rifampin 600 mg QD and clofazimine 750 mg BID versus clarithromycin 1000 mg BID, rifabutin 600 mg QD and ethambutol 15 mg/kg QD in HIV+patients with Mycobacterium a
Daunoxome-phase II trial for epidemic Kaposi's sarcoma (EKS).: Int Conf AIDS. 1994 Aug 7-12;10(1):18 (abstract no. 046B). Unique Identifier : AIDSLINE ICA10/94369588; Wernz JC; Taubes B; Friedberg A; NYU Kaplan Cancer Center, NY.; In January 1993 accrual began at NYU Medical Center using the investigational drug Daunoxome for previously treated patients with progressive EKS. As of February 18, 1994 42 patients had been entered on study. Daunoxome is a combination of daunorubicin and a phospholipid vesicle carrier (liposome) specifically develop
Incidence of Kaposi's sarcoma among PWAIDS over time: is it decreasing? The Spanish Study Group on AIDS and Cancer.: Int Conf AIDS. 1994 Aug 7-12;10(1):18 (abstract no. 047B). Unique Identifier : AIDSLINE ICA10/94369589; Casabona J; Blanch C; Castilla J; Podzamczer D; Riera S; Rubio R; Iravessera de les Corts, Barcelona.; OBJECTIVE: To identify temporal trends of AIDS associated Kaposi s sarcoma (KS) in Spain . METHODS: Data from 18.256 AIDS cases reported to the Spanish AIDS Registry from 1981 to 1992 and data from a retrospective cohort of AIDS patients from three different Autonomous Communities (M
Intensive chemotherapy and G-CSF in HIV-associated non-Hodgkin's lymphoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):18 (abstract no. 044B). Unique Identifier : AIDSLINE ICA10/94369590; Oksenhendler E; Dubreull ML; Gerard L; Barateau V; Levy Y; Deforges L; Ferchal F; Clauvel JP; Hopital Saint-Louis, Paris, France.; OBJECTIVE: To evaluate efficacy, safety and virological impact of intensive chemotherapy associated with G-CSF in HIV-associated NHL. PATIENTS: 33 HIV+ patients with aggressive NHL and CD4+ cells > or = 100. CDC group IV: 10; mean CD4: 344 (100-910). Burkitt: 16, large cell: 10, immunoblastic: 4, anaplastic: 3; stage
Pathogenesis of AIDS associated large cell lymphoma.: Int Conf AIDS. 1994 Aug 7-12;10(1):18 (abstract no. 045B). Unique Identifier : AIDSLINE ICA10/94369591; Tsuzuki A; Herndier B; Marsh J; Ng V; Nolan T; Shiramizu B; McGrath M; University of California, San Francisco.; OBJECTIVE: The pathogenesis of HIV associated Burkitt s lymphoma probably revolves around dysregulation of the c-myc proto-oncogene. For other AIDS associated lymphomas, particularly the large cell lymphomas (LCL), the pathogenesis is unclear, particularly when some pre-lymphomatous states or even frank lymphomas are
Development of oral lesions in sexually and parenterally HIV-1 infected persons. The Oral AIDS Center Epidemiology Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):180 (abstract no. PB0145). Unique Identifier : AIDSLINE ICA10/94369592; Hilton JF; Lifson AR; Canchola AJ; Greenspan D; Greenspan JS; University of California, San Francisco.; OBJECTIVE: (1) To compare incidence of oral lesions among HIV-1 infected persons by route of transmission of HIV (sexual or parenteral). (2) To estimate the effect of oral lesions by group on the incidence of AIDS. METHODS: We examined 76 HIV-1 infected homosexual men and 72 men and 24 women infected through contamina
Early attention of oral infections.: Int Conf AIDS. 1994 Aug 7-12;10(1):180 (abstract no. PB0146). Unique Identifier : AIDSLINE ICA10/94369593; Ruiz O; Feregrino M; Alexis A; Clinica Isi de Norteamerica, Mexico.; OBJECTIVES: To assail the importance of the opportune diagnostic of the secondary infections on the oral cavity, that are present in the HIV infection, for the survival of the patients, as much in quality of life, as quantity of life. METHOD We was studied 189 patients with HIV in differents stage of infections. We wa
Follow up of oral manifestations in HIV/AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):180 (abstract no. PB0147). Unique Identifier : AIDSLINE ICA10/94369594; Korchma E; Lelkes M; Banhegyl D; Miskovits E; Saint Laszlo Hospital, Budapest, Hungary.; OBJECTIVE: Correlation of oral manifestations and disease progression was of was examined. METHODS: Between 1989-1993 all Hungarian patients (pts) with symptomatic HIV/AIDS were provided dental and oral health care and follow up along with clinical and laboratory care. On oral cavity examination cytology, virology, mi
Oral manifestations of HIV infection in Japanese.: Int Conf AIDS. 1994 Aug 7-12;10(1):180 (abstract no. PB0148). Unique Identifier : AIDSLINE ICA10/94369595; Motegi N; Tokyo Metropolitan Komagome Hospital, Japan.; OBJECTIVE: Since the first descriptions of Acquired immunodeficiency syndrome (AIDS) in 1981, the infection has been characterized as having significant oral manifestation in adult patient. But, we have less of these cases in Japan than in U.S.A. Therefore we present some cases of s
Oral candidiasis, the steady partner's treatment.: Int Conf AIDS. 1994 Aug 7-12;10(1):181 (abstract no. PB0150). Unique Identifier : AIDSLINE ICA10/94369596; Rosello X; Lopez J; Jane E; University of Barcelona, Spain.; OBJECTIVES: To determine the importance of treating the steady couple of HIV (+) patients to prevent the oral candidiasis recurrences. METHODS: 70 HIV (+) patients with CD4 levels
Oral mycoplasma and Candida from HIV infected (+) non-AIDS subjects.: Int Conf AIDS. 1994 Aug 7-12;10(1):181 (abstract no. PB0151). Unique Identifier : AIDSLINE ICA10/94369597; Rowland RW; Blankenship P; Dobis L; Gunsolley J; Moray L; MCV/VCU, Richmond.; OBJECTIVE: To assess the subclinical presence of mycoplasma and candida in the oral cavity of HIV infected asymptomatic (non-AIDS) patients. METHODS: Supra and subgingival plaque samples, hard and soft palate, and dorsal tongue swabs were collected from 17 HIV(+) non-AIDS subjects and 10 HIV(-) controls. No subject pr
Prognostic value of fluconazole response patterns in HIV infected patients with oropharyngeal candidosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):181 (abstract no. PB0149). Unique Identifier : AIDSLINE ICA10/94369598; Diz DP; Miralles C; Ocampo A; Feujoo JF; Sopena B; De la Fuente J; Martinez C; Xeral-Cies Hospital, Vigo, Spain.; OBJECTIVE: To establish a relation between HIV infection evolution and fluconazole response patterns in HIV-positive patients with oropharyngeal candidosis. METHODS: In a prospective study a 30 HIV-positive cohort with clinical and microbiological confirmed oropharyngeal candidosis, received fluconazole therapy (100 m
Ultrastructural study of oral hairy leukoplakia.: Int Conf AIDS. 1994 Aug 7-12;10(1):181 (abstract no. PB0152). Unique Identifier : AIDSLINE ICA10/94369599; Sugihara K; Yamashita S; Kagoshima University Dental School, Japan.; OBJECTIVE: The purpose of this study was to investigate the ultrastructure of oral hairy leukoplakia (HL) in HIV-positive hemophiliacs in Japan . METHODS: Biopsy specimens for the ultrathin sectioning were taken from HL developed in the lateral border of the tongue of two HIV-positiv
CD4+ and CD8+ lymphocytes in gingival tissue from HIV-infected persons.: Int Conf AIDS. 1994 Aug 7-12;10(1):182 (abstract no. PB0156). Unique Identifier : AIDSLINE ICA10/94369600; Odden K; Schenck K; Hurlen B; Dental Faculty, University of Oslo, Norway.; OBJECTIVE: The aim of the present study was to examine whether the general HIV-related decline in CD4+ T-lymphocytes and CD4+/CD8+ ratio is reflected in gingival tissues, and thus can constitute a possible explanation of HIV-infected persons susceptibility to periodontal diseases. METHODS: Frozen sections of gingival
Epidemiology of linear gingival erythema in HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):182 (abstract no. PB0155). Unique Identifier : AIDSLINE ICA10/94369601; Konzelman JL; Rams TE; Swango PA; Kleinman DV; Nowjack-Raymer RE; Henry M. Jackson Foundation, Washington, DC 20307.; Periodontal tissues were evaluated for linear gingival erythema in 724 HIV-seropositive and 275 demographically similar HIV-seronegative military personnel at Walter Reed Army Medical Center. Positive scores were recorded for facial and lingual surfaces of marginal gingival tissues exhibiting a continuous > or = 1 mm
Oral hairy leukoplakia (OHL): topical use of trichloroacetic and glycolic acids--results.: Int Conf AIDS. 1994 Aug 7-12;10(1):182 (abstract no. PB0153). Unique Identifier : AIDSLINE ICA10/94369602; Nishio CE; Petri V; Narahashi E; Department of Dermatology, Escola Paulista de Medicina, SP,; Brazil.; OBJECTIVE: to investigate perspectives of control of oral hairy leukoplakia (OHL) of HIV-seropositive patients. METHODS: 10 HIV-seropositive individuals were submitted to trichloroacetic acid (TCA) 40% and 10 other (HIV-seropositive) to glycolic acid (GA) 30%, topically. RESULTS: all patients reported good results if
Site-specific relationship between oral spirochaetes and periodontal disease in human immunodeficiency infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):182 (abstract no. PB0154). Unique Identifier : AIDSLINE ICA10/94369603; Wanzala P; Riviere G; Lukehart S; Kreiss J; DeRouen T; Ndinya A; Kenya Medical Research Institute.; OBJECTIVES: A Study of the distribution of Oral Spirochaetes in HIV infected and uninfected individuals and their etiological significance in periodontal disease. METHODS: 59 patients were included in the study. Loss of attachment was measured at all the sites of teeth present, Plaque samples from the 6 most affected
An ethno-epidemiological study of the oral manifestations of HIV/AIDS patients in South Africa.: Int Conf AIDS. 1994 Aug 7-12;10(1):183 (abstract no. PB0161). Unique Identifier : AIDSLINE ICA10/94369604; Sauer G; Arendorf TM; Wood R; O'Keefe E; OBJECTIVE: To record the prevalence of oral manifestations of HIV/AIDS in South Africa which has not been previously recorded and note ethnic differences. METHODS: Two hundred consecutive HIV/AIDS patient of varying ethnic origin presenting at a specialised clinic were studied. Or
Experience on 584 HIV+ patients of a dental referral center.: Int Conf AIDS. 1994 Aug 7-12;10(1):183 (abstract no. PB0162). Unique Identifier : AIDSLINE ICA10/94369605; Silva Junior A; Ferreira S; Perez MA; Brilhante E; Camillo R; Torres S; Dental School/NESC/UFRJ, Brazil.; INTRODUCTION: To describe oral lesions most commonly seen in HIV+ as well as their most relevant social features. METHODS: 584 HIV+ patients charts were reviewed from a dental clinic. Full medical history was obtained and an intraoral exam was performed on the first day of admission. Data such as education, profession
Stomatitis aphthous in patients HIV+, treatment.: Int Conf AIDS. 1994 Aug 7-12;10(1):183 (abstract no. PB0159). Unique Identifier : AIDSLINE ICA10/94369606; Lopez J; Rosello X; Jane E; University of Barcelona, Spain.; OBJECTIVES: Evaluate the therapeutic value of abeclametosone hemissuccinate and neomicine in solution, to decrease the symptoms and time duration of the recurrent stomatitis aphthous. METHOD: After combining both products we studied 50 patients with major aftas and herpetic stomatitis. This is a double-blind study to
Treatment of aphthous oral ulcers with pentoxifylline.: Int Conf AIDS. 1994 Aug 7-12;10(1):183 (abstract no. PB0158). Unique Identifier : AIDSLINE ICA10/94369607; Navarro A; Pizarro A; Herranz P; Aguayo M; Leon M; Pujol E; Hospital Jr Jimenez, Huelva, Spain.; OBJECTIVE: Patients infected with human immunodeficiency virus (HIV) frecuently have aphtous ulcers (AO) of the oral mucosa. Successful use of pentoxifylline has been reported recently in the treatment of AO in general population. The purpose of this study was to evaluate the efficacy of pentoxifylline in HIV patients
Initial report of CD4 counts and oral lesions in a Texas HIV seropositive cohort.: Int Conf AIDS. 1994 Aug 7-12;10(1):184 (abstract no. PB0164). Unique Identifier : AIDSLINE ICA10/94369608; Yeh CK; Puttaiah R; Fabre C; Dove S; Cottone J; UTHSCSA, San Antonio 78284-7919.; OBJECTIVE: A prospective surveillance program at an HIV/AIDS dedicated dental clinic has been developed to monitor HIV associated oral lesions in relationship to patients general health. This is a preliminary report of the baseline data on disease characteristics of dental patients being treated at this clinic. METHO
Oral lesions in patients with HIV infection enrolled in CPCRA trials. The Terry Beirn CPCRA. Community Programs for Clinical Research on AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):184 (abstract no. PB0165). Unique Identifier : AIDSLINE ICA10/94369609; Torres R; Tobin J; Vaughn A; Podhurst L; Bolden B; Raschella N; St. Vincent's Hospital, NYC.; OBJECTIVE: Oral candidiasis (OC) and hairy leukoplakia (HL) are oral lesions which have been shown to be markers of HIV disease progression. Using the Community Programs for Clinical Research on AIDS (CPCRA) observational and clinical trials database, this study evaluates the incidence and prevalence or these oral les
Oral manifestations of HIV infection in Campinas City.: Int Conf AIDS. 1994 Aug 7-12;10(1):184 (abstract no. PB0163). Unique Identifier : AIDSLINE ICA10/94369610; Bertazzoli R; Lourenco C; Corsini Center, Campinas, Sao Paulo, Brazil.; OBJECTIVES: To determine the prevalence, type and relationship to the clinical stage of oral mucosal lesions associated with HIV infection at the Corsini s multidisciplinary Center at the first visit. METHODS: Oral exam were performed in one hundred and nine HIV-1 infected persons from January to December 1993. Oral c
Prolonged healing period of the extracted wound. Report of a case.: Int Conf AIDS. 1994 Aug 7-12;10(1):184 (abstract no. PB0166). Unique Identifier : AIDSLINE ICA10/94369611; Maeda N; Mizobe J; Kohshikai Dental Clinic, Osaka, Japan.; OBJECTIVE: In order to avoid the complications of the patients after the extractions of the teeth and surgical treatments, dentist should understand the conditions of the patients and be care of them as earier as possible on the infection stage of HIV. METHODS: Clinical case of the dental treatment of the patients suf
Cross-contamination risks of invasive dental procedures using high-speed handpieces.: Int Conf AIDS. 1994 Aug 7-12;10(1):185 (abstract no. PB0167). Unique Identifier : AIDSLINE ICA10/94369612; Ito M; Ashoori M; Osuka S; Matsuyama M; Usami T; Kaneda T; Dept. Oral Surgery, Nagoya University Sch. Medicine, Japan.; OBJECTIVE: Oral surgeons use high-speed handipieces for various invasive dental procedures to HIV infected patients. This study investigated the incidence and extent of blood contamination of handpieces used in invasive dental procedures. METHODS: Water samples were collected from high-speed handpieces used for the ex
Endoscopy studies of AIDS: the 90's versus the 80's.: Int Conf AIDS. 1994 Aug 7-12;10(1):185 (abstract no. PB0170). Unique Identifier : AIDSLINE ICA10/94369613; Bernal A; Frazier R; Del Junco G; Gathe J Jr; Piot D; Special Diseases Unit, Park Plaza Hospital, Houston, Texas.; OBJECTIVE: A comparative study of the endoscopic findings of AIDS in the 90 s vs. the early 80 s. Those years precluded antiretroviral therapy as well as most of the primary and secondary prophylaxis of opportunistic infections. To analyze the impact, if any, of those measures on the epidemiology and clinical spectrum
Oral manifestations of HIV/AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):185 (abstract no. PB0168). Unique Identifier : AIDSLINE ICA10/94369614; Ssali C; INTRODUCTION: Considering oral manifestations like oral thrush , Kaposis s Sarcoma in the Clinical diagnosis of HIV/AIDS. Red Lips is a clinical Feature which one should not over look in the oral examination of HIV/AIDS patients. METHODOLOGY: This is based on taking history and
Upper digestive tract endoscopy in HIV infected patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):185 (abstract no. PB0169). Unique Identifier : AIDSLINE ICA10/94369615; Aoki FH; Meirelles JO; Monteiro DT; Lima J; Abreu W; Pavan M; Inf. Dis. Division-FMSc-St. Univ. of Campinas-Cps-SP-Brazil.; OBJECTIVES: To evaluate the main injuries observed in upper digestive tract endoscopy in patients infected with HIV, histopathologic changes and infectious agents found in the biopsy samples. METHODS: A retrospective analysis on one hundred endoscopies from 83 patients who were infected with HIV and followed in the In
Carbohydrate malabsorption in patients with HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):186 (abstract no. PB0173). Unique Identifier : AIDSLINE ICA10/94369616; Ginaldi L; Furia N; DiGiammartino D; Marani-Toto G; Corazza GR; Quaglino D; Department of Int. Med., University of L'Aquila, Italy.; Patients with human immunodeficiency virus infection frequently have intestinal dysfunction, such as abdominal pain, diarrhea and weight loss, often associated with opportunistic infections or secondary malignancies. We utilized breath hydrogen measurements to determine the prevalence of carbohydrate malabsorption and
CMV gastrointestinal disease and Salmonella sepsis in AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):186 (abstract no. PB0174). Unique Identifier : AIDSLINE ICA10/94369617; De Agostini M; Lipani F; Sinicco A; Inst. of Infect Dis, Univ. of Turin, Italy.; OBJECTIVE: To describe manifestations of CMV gastrointestinal disease and to evaluate correlations between CMV infection and non-typhi Salmonella sepsis. METHODS: Retrospective chart review by clinicians following the patients prospectively. RESULTS: 8 AIDS pati
Human papilloma virus in the esophagus in HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):186 (abstract no. PB0171). Unique Identifier : AIDSLINE ICA10/94369618; Bouchaud O; Marche C; Cadiot G; Longuet P; Rene E; Coulaud JP; Bichat Claude Bernard Hospital, Paris, France.; OBJECTIVE: Esophagitis (E) in AIDS patients (pts) remain frequently without etiology. We would like to show that Human Papillomavirus (HPV) may be found in the esophagus and to discuss HPV as a possible agent in E. METHODS: We performed a prospective study in pts having had an upper endoscopy. Pts were divided in 3 gr
Impaired gastric empyting in patients with HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):186 (abstract no. PB0172). Unique Identifier : AIDSLINE ICA10/94369619; Piot D; Gathe J Jr; Bernal A; Stool E; Clemmons J; Stenoien R; Goldstein J; Park Plaza Hospital, Special Diseases Unit, Houston, Texas.; OBJECTIVE: To detail characteristics of HIV infected patients with gastric emptying disturbances (GED). METHODS: Retrospective chart review of 18 patients followed prospectively by the authors. GED was documented by nuclear medicine gastric emptying study. RESULTS: Patient characteristics include: Age range 23-63; 16
AIDS associated diarrhea. Preliminary results of a study.: Int Conf AIDS. 1994 Aug 7-12;10(1):187 (abstract no. PB0175). Unique Identifier : AIDSLINE ICA10/94369620; Tumbarello M; Ventura G; Tacconelli E; Scoppettuolo G; Papi G; Cauda R; Istituto Malattie Infettive, Universita Cattolica Roma, Italy.; OBJECTIVE: To evaluate the frequency, microbiological agents, clinical findings and impact on the quality of life of diarrhea in AIDS patients (pts). METHODS: All consecutive AIDS pts admitted to our ward from 7-1993 to 2-1994 were included in the study. Diarrhea was defined as 3 or more daily bowel movements for more
Etiology of acute abdomen in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):187 (abstract no. PB0176). Unique Identifier : AIDSLINE ICA10/94369621; Figueroa R; Espinosa RF; Santillan MA; Cruz MA; Hospital de Infectologia, Centro Medico la Raza, Mexico City.; OBJECTIVE: To report the clinical characteristics and the etiology of acute abdomen in a group of AIDS patients, who had been submitted to exploratory laparotomy. METHODS: Retrospective study compresing the period from march 1st, 1988 to june 30, 1993, at the Hospital de Infectologia, IMSS, in Mexico City. We reviewed
Etiology, nutritional assessment and management of chronic diarrhea in 24 HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):187 (abstract no. PB0178). Unique Identifier : AIDSLINE ICA10/94369622; Wanke C; Pleskow D; DeGirolami P; Federman M; New England Deaconess Hospital, Boston, MA.; OBJECTIVE: We studied 24 male patients (p) with HIV who had chronic diarrhea. METHODS: Each p had a nutritional survey, stool cultures and exam including cryptosporidium (cryp) and microsporidium (mic), upper and lower endoscopy with biopsy, electron microscopy, viral cultures and quantitative small bowel cultures. RE
Experience of two years in therapy of AIDS hypersecretory diarrhea with octreotide.: Int Conf AIDS. 1994 Aug 7-12;10(1):187 (abstract no. PB0177). Unique Identifier : AIDSLINE ICA10/94369623; Miranda-Ruiz R; Feregrino-Goyos M; Alvarado Diez R; Castanon GJ; Gallegos-Perez H; Eid-Lidt G; Hospital Especialidades del Centro Medico siglo XXI del IMSS,; Mexico D.F., Mexico.; OBJECTIVES: Evaluation of the use of octreotide(analog to long range action synthetic somatostatin) in therapy of severe and chronic hypersecretory diarrhea from AIDS (with more than 10 daily evacuations and 2 to 7 liters of daily volume of feces)compared to a similar control group. METHODS: 90 HIV patients were studi
Chronic bacterial enteropathy in AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):188 (abstract no. PB0180). Unique Identifier : AIDSLINE ICA10/94369624; Giang TT; Kotler DP; Orenstein JM; St. Luke's/Roosevelt Hosp. Ctr., New York, NY 10025.; OBJECTIVE: To determine the prevalence and clinical correlations of adherent bacteria in intestinal biopsies. METHODS: Biopsies from 66 HIV-infected individuals undergoing endoscopy in 1991 were reviewed blindly. Light and electron microscopic detection of adherent bacteria, their localization in the intestine, and th
Clinical significance of hyperamylasemia in HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):188 (abstract no. PB0181). Unique Identifier : AIDSLINE ICA10/94369625; Merino E; Reus S; Sanchez J; Trigo C; Boix V; Portilla J; Perez-Mateo M; Hospital General Universitario de Alicante, Spain.; OBJECTIVE: To investigate the origin and clinical significance of hyperamylasemia in HIV patients. METHODS: We have studied HIV patients with hyperamylasemia (> 220UI/L) admitted at our unit between Oct-92 and Jan-94. We investigated history of pancreatitis, alcohol intake and previous drugs. Liver and renal function
Hepatobiliary disorders in HIV-patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):188 (abstract no. PB0182). Unique Identifier : AIDSLINE ICA10/94369626; Verdejo J; Iniguez A; Polo R; Martinez ML; Inchaustegui L; Enriquez A; Gonzalez-Lahoz JM; Instituto de Salud Carlos III, Madrid, Spain.; We evaluate hepatobiliary complications and serological markers in viral hepatitis in a prospective study since February 1992. RESULTS: Patients 108 (51 AIDS-CDC 1993). Average follow-up 245 days (range 60-690). Sex: M 81/F 27. Average age 31.8 (range 21-69). Risk group: IVDU 72, Homosexual 27, Heterosexual 8, Transfu
VIP and refractory diarrhea in AIDS/ARC patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):188 (abstract no. PB0179). Unique Identifier : AIDSLINE ICA10/94369627; Manfredi R; Mastroianni A; Coronado O; Vezzadini P; Fanti MP; Chiodo F; University of Bologna, Italy.; OBJECTIVE. We have previously described 3 AIDS patients (p) with chronic idiopathic diarrhea associated with elevated vasoactive intestinal peptide (VIP) secretion (AIDS 1993; 7:223-6). Aim of this study is to evaluate a series of AIDS/ARC p with intractable infectious and noninfectious diarrhea. METHODS AND RESULTS.
Comparison of HIV viral burden in peripheral blood and rectal mucosa.: Int Conf AIDS. 1994 Aug 7-12;10(1):189 (abstract no. PB0186). Unique Identifier : AIDSLINE ICA10/94369628; Reka S; Kotler DP; St. Luke's-Roosevelt Hosp, New York, NY 10025.; OBJECTIVE: To compare HIV proviral DNA burden by polymerase chain reaction (PCR) in mononuclear cells isolated from peripheral blood (PBL) and rectal mucosa lamina propria (LPL), and HIV RNA by in situ hybridization in tissue biopsies. METHODS: 15 HIV-infected individuals were studied. Quantitative DNA PCR was perform
G-CSF induced hepatitis and pancreatitis in a HIV-seropositive patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):189 (abstract no. PB0184). Unique Identifier : AIDSLINE ICA10/94369629; Zylberberg L; Zylberberg H; Havard S; Hagege H; Franck N; Escande JP; Tarnier Hospital, Paris, France.; Among the side effects induced by the granulocyte colony-stimulating factor (G-CSF) acute hepatitis with histological confirmation and biologic pancreatitis have never been reported. We report a case of 35 years old HIV seropositive homosexual male who had no history of hepatobiliary disorder nor alcohol abuse. He rec
Liver disease in patients with AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):189 (abstract no. PB0183). Unique Identifier : AIDSLINE ICA10/94369630; Silva MA; Tannuri AA; Lobo AL; Costa RS; Mello CE; Lima MB; Oliveira CA; University of Rio de Janeiro, Brazil.; OBJECTIVE: The evaluation of clinical, biochemical, serological and morphological data of liver involvement in high risk groups with AIDS was the aim of this work. Our purpose was to determine the spectrum of liver disease in AIDS and the clinic morphological correlation in order to ascertais if any clinical or phatol
Preferential depletion of rectal lamina propria CD4+ lymphocytes.: Int Conf AIDS. 1994 Aug 7-12;10(1):189 (abstract no. PB0185). Unique Identifier : AIDSLINE ICA10/94369631; Clayton F; Reka S; Kotler DP; St. Luke's/Roosevelt Hosp Ctr, New York, NY 10025.; OBJECTIVE: To compare changes in mucosal lymphoid populations during disease progression. METHODS: We performed quantitative histologic and immunohistochemical stains in 61 subjects; 7 with CD4 > 500 (Early), 24 with CD4
Combined p24 antigen and anti-p66 as markers of AIDS-free time.: Int Conf AIDS. 1994 Aug 7-12;10(1):19 (abstract no. 050B). Unique Identifier : AIDSLINE ICA10/94369632; Henrard D; Meiszberg T; Wiesner D; Goedert J; Abbott Labs, Abbott Park, IL.; OBJECTIVE: Assess levels of specific HIV-1 antibodies as early predictor of AIDS-free time, especially in p24-antigen (Ag) negative subjects. METHODS: Two to ten (mean 5.5) annual samples from 30 homosexual men with documented seroconversion dates were studied. Seven men developed AIDS in 3 years (group I), 11 develop
First HIV-1 positive diagnosis coincident with AIDS: presentation and prognosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):19 (abstract no. 051B). Unique Identifier : AIDSLINE ICA10/94369633; Poznansky MC; Coker R; Skinner C; Bailey S; Renton A; Weber J; St Mary's Hospital, London, U.K.; OBJECTIVE: Between January 1991 and December 1992, 61/281 (22%) of the patients presenting with their first AIDS defining illness to the Genito-Urinary Medicine Dept. at St Mary s Hospital in London had not previously been diagnosed as being HIV-1 positive. This study sought to determine whether late presenters with H
HIV/AIDS related neoplasms in the population of Genoa, Italy.: Int Conf AIDS. 1994 Aug 7-12;10(1):19 (abstract no. 048B). Unique Identifier : AIDSLINE ICA10/94369634; Crovari P; Canessa A; De Rosa MG; Donnini R; Orengo A; Piersantelli N; Rizzo F; Vercelli M; Inst. Hygiene and Prev. Med., Genoa Univ., Italy.; OBJECTIVE: During 1993 a collaborative study programme was set up to evaluate HIV/AIDS related neoplasms in Genoa, Italy . Research was carried out by the Regional Epidemiologic Observatory, the Ligurian Tumour Registry, the Regional Mortality Registry and three Infectious Diseases D
Studies on HIV infected individuals who are long term non-progressors. Multicenter AIDS Cohort Study (MACS).: Int Conf AIDS. 1994 Aug 7-12;10(1):19 (abstract no. 049B). Unique Identifier : AIDSLINE ICA10/94369635; Graziosi C; Demarest JF; Vaccarezza M; Cohen OJ; Pantaleo G; Fauci AS; NIAID, National Institutes of Health, Bethesda, MD 20892.; OBJECTIVE: To analyze viral burden and viral replication in peripheral blood (PB) and lymph node (LN) from HIV infected individuals who are long term non-progressors (LTNP). METHODS: Viral burden (i.e. frequency of HIV infected cells) and viral replication were assessed by semiquantitative DNA and RNA polymerase chain
A survey on the oral lesions of HIV/AIDS patient in Japan. (Report 1). Research Committee on Prevention of Developing Ignesies and Therapy for HIV Infected Patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):190 (abstract no. PB0190). Unique Identifier : AIDSLINE ICA10/94369636; Ikeda M; Maeda N; Dept. of Dentistry, Kanagawa Children's Medical Center, Kanagawa,; Japan.; OBJECTIVE: In Japan , there are 2914 people with HIV and 685 AIDS patients as of the end of December 1993. Among these, the cause of infection consisted of hemophillia in 61%, heterosexials in 18%, homosexials in 8%, IV drug users in 0.3%, mother to child 0.2%, and 12% others. In ord
Itraconazole as secondary prophylaxis against oral/oesophageal candidosis in patients with AIDS or ARC.: Int Conf AIDS. 1994 Aug 7-12;10(1):190 (abstract no. PB0188). Unique Identifier : AIDSLINE ICA10/94369637; Smith D; Gazzard B; Kenny DW; Community HIV/AIDS Trials Network, Sydney, New South Wales,; Australia.; OBJECTIVE: To assess the efficacy and toxicity of Itraconazole (200 mg od) in the prevention of recurrent Oral and/or Oesophageal candidosis in patients with AIDS or ARC. METHODS: Seventy patients with ARC or AIDS and a diagnosis of Oral/Oesophageal Candida, confirmed by clinical and mycological assessment, undertook
Pathogenicity of Entamoeba histolytica in Japanese male homosexual population.: Int Conf AIDS. 1994 Aug 7-12;10(1):190 (abstract no. PB0189). Unique Identifier : AIDSLINE ICA10/94369638; Takeuchi T; Kobayashi S; Okusawa E; Tachibana H; Takada S; Ohtomo H; Department of Tropical Medicine and Parasitology, School of; Medicine, Keio University, Tokyo, Japan.; OBJECTIVE: In contrast to Western countries, there have been many cases with invasive sexually transmitted amebiasis in Japan . This study was attempted to delineate its epidemiological characteristics and clarify the pathogenicity of the ameba isolates from Japanese homosexual popul
Refractory Eschericia coli 0157:H7 associated hemolytic uremic syndrome (HUS) and HIV-infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):190 (abstract no. PB0187). Unique Identifier : AIDSLINE ICA10/94369639; Albrecht H; Stellbrink HJ; University of Hamburg, Germany.; BACKGROUND: Thrombotic microangiopathies (TMA) are characterized by a pentad of microangiopathic hemolytic anemia, thrombocytopenia, fever, central nervous system abnormalities, and renal dysfunction. 2 distinct forms of TMA exist: Thrombotic thrombocytopenic purpura (TTP), characterized by minimal kidney dysfunction
Acute paralysis of the serratus muscle in an HIV positive patient treated by DDC.: Int Conf AIDS. 1994 Aug 7-12;10(1):191 (abstract no. PB0193). Unique Identifier : AIDSLINE ICA10/94369640; Faller JP; Ziegler F; Balblanc JC; Labouret P; Ruyer O; Centre Hospitalier, Belfort, France.; OBJECT: to document paralysis of the serratus muscle as a manifestation of HIV infection in AIDS with no other neuropathical manifestations. A 35 years old HIV infected man (risk factor: homosexual, stage II B, CD4 cell count: 192) had been on DDC therapy for 2 months when he was evaluated for a one week history of ac
Pathology of the central nervous system of AIDS in Japan.: Int Conf AIDS. 1994 Aug 7-12;10(1):191 (abstract no. PB0191). Unique Identifier : AIDSLINE ICA10/94369641; Funata N; Koike M; Tokyo Metropolitan Komagome Hospital, Japan.; OBJECTIVE: Autopsy cases of AIDS were reviewed to find the characteristic features in the pathology of the central nervous system (CNS) in Japanese patients. In addition, we examined the presence of Epstein-Barr virus (EBV) in CNS malignant lymphomas. METHODS: A consecutive autopsy series of 35 patients was reviewed.
Psychoneurologic disorders and evaluation of ability to work in HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):191 (abstract no. PB0192). Unique Identifier : AIDSLINE ICA10/94369642; Ivanov KS; Lobzin YV; Zhdanov KV; Department of infectious diseases, Medico-Military Academy, St.; Petersburg, Russia.; OBJECTIVE: Determination of psychoneurologic disorders character and degree and evaluation of ability to work in HIV-infected patients. METHODS: 166 individuals at various stages of HIV infection without cerebral opportunistic infections and tumors have been studied. Clinical neurologic (including CT) and special psyc
Stroke in young HIV patients: association with cocaine abuse and meningitis.: Int Conf AIDS. 1994 Aug 7-12;10(1):191 (abstract no. PB0194). Unique Identifier : AIDSLINE ICA10/94369643; Qureshi AI; Akbar MS; McGaffigan S; Weissman J; Frankel M; Janssen R; OBJECTIVE: To determine the prevalence and etiology of stroke in HIV+ patients. METHODS: We conducted a retrospective review of medical records of all HIV+ patients admitted to a US public hospital, with diagnosis of stroke during 1989-1993. RESULTS: Of 1171 HIV+ admissions, 36 (3%, mean age 38 years) were admitted wi
BAEPs in early stages of HIV-infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):192 (abstract no. PB0196). Unique Identifier : AIDSLINE ICA10/94369644; De la Fuente J; Rodriguez E; Ocampo A; Miralles C; Sopena B; Morena JA; Martinez C; Dept. Internal Medicine, Hospital Xeral, Vigo, Spain.; OBJECTIVE: To compare the brain-stem auditory evoked potentials (BAEPs) of HIV-infected subjects in early stages with normal healthy controls. METHODS: BAEPs were performed on 23 otoneurological asymptomatic HIV-1 seropositive patients and 30 HIV-1 seronegative controls. Abnormal audiometry, alcohol consumption, AIDS-
HIV independent factors influencing neuropsychological performance in ARC and AIDS individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):192 (abstract no. PB0198). Unique Identifier : AIDSLINE ICA10/94369645; Dally LG; Chiesi A; Tomino C; Floridia M; Seeber A; Vella S; ISS, Lab. of Virology, Rome, Italy.; INTRODUCTION: Our previous studies on the epidemiology of ADC in Italy (16813 pts. diagnosed between August 1987 and December 1993) showed that ADC, as the first AIDS defining disease, occurred in 9.1% IVDU s vs 5.2% homosexuals, the two groups being similar for mean CD4+ cell count
Psychiatric disorders in HIV patients: hospitalized vs. non-hospitalized.: Int Conf AIDS. 1994 Aug 7-12;10(1):192 (abstract no. PB0197). Unique Identifier : AIDSLINE ICA10/94369646; Prenzlauer SL; Bialer PA; Bogdonoff L; Kato KS; Wilets I; Beth Israel Med Ctr, NY, NY 10003.; OBJECTIVE: To determine the prevalence of psychiatric disorders in inpatient PWA s (IPWA) v outpatient (OPWA), and inpatient HIV+ asymptomatic (IHIV+) patients v outpatient (OHIV+). METHOD: Psychiatric consultations were performed on 675 IPWA and IHIV+ and 155 OPWA and OHIV+ from 1989-93. Diagnoses were based upon DSM
Psychological approach to AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):192 (abstract no. PB0195). Unique Identifier : AIDSLINE ICA10/94369647; Costa LM; Azevedo RC; Estadual University of Rio de Janeiro, Brazil.; OBJECTIVES: The multidisciplinarian attendance and the patient s life graph study, their relationships, the specific situation during the disease s early stage, evaluating how psychological factors may influence in this moment. At the same time, to observe the importance of approaches such as: familiar relationship, h
HIV-1-gp120 causes induction of neuronal injury in vitro: prevention by memantine.: Int Conf AIDS. 1994 Aug 7-12;10(1):193 (abstract no. PB0202). Unique Identifier : AIDSLINE ICA10/94369648; Muller WE; Bormann J; Schroder HC; Ushijima H; Institut fur Physiologische Chemie, Mainz, Germany.; OBJECTIVES: Besides lymphocytes also other cells like astrocytes are infected by HIV-1 in vitro. Primary neurons are very rarely, if at all infected by HIV-1. MATERIALS AND METHODS: Rat cortical cell cultures were prepared from the brains of newborn Wistar rats. After 48 h in culture the cells were used for the experi
Progressive lesion of ascending sensory tracts.: Int Conf AIDS. 1994 Aug 7-12;10(1):193 (abstract no. PB0201). Unique Identifier : AIDSLINE ICA10/94369649; Husstedt IW; Grotemeyer KH; Busch H; Hoffmann U; Zidek W; Department of Neurology, Westf. Wilhelms-Universitat,; Munster/Germany.; OBJECTIVE: It was the aim of our study to detect whether or not the lesion of the ascending central and peripheral sensory tracts in HIV-infected patients are progressive. METHODS: 36 patients (mean age 35.4 +/- 4.6 years, HIV-infection since 5.6 +/- 1.8 years (laboratory diagnosis), 9 female, 27 male) were examined c
Risk of neuropsychological impairment steadily rises from date of seroconversion: HNRC experience.: Int Conf AIDS. 1994 Aug 7-12;10(1):193 (abstract no. PB0200). Unique Identifier : AIDSLINE ICA10/94369650; Grant I; Heaton RK; Deutsch R; McCutchan JA; Atkinson JH; Chandler J; University of California, San Diego, La Jolla.; OBJECTIVE: Neuropsychological (NP) impairment is prevalent in persons with AIDS, but the natural course of such impairment is poorly understood. We explored risk of NP impairment as a function of years since seroconversion. METHODS: The 152 non-intravenous drug using HIV+ men all had documented dates of negative and p
Subclinical motor deficits in HIV infection are not influenced by intravenous drug abuse.: Int Conf AIDS. 1994 Aug 7-12;10(1):193 (abstract no. PB0199). Unique Identifier : AIDSLINE ICA10/94369651; Giesen HJ; Roick H; Hefter H; Jablonowski H; Freund HJ; Arendt G; Department of Neurology, University of Dusseldorf, Germany.; OBJECTIVE: Electrophysiological evaluation of individual motor performance is an appropriate means to quantify subclinical motor deficits in HIV Infection. These deficits may later on result in the HIV associated cognitive/motor complex. We wanted to find out whether motor performance is influenced by intravenous drug
HIV-1 associated cognitive-motor complex in Europe. The AIDS in Europe Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):194 (abstract no. PB0204). Unique Identifier : AIDSLINE ICA10/94369652; Chiesi A; Dally LG; Vella S; Lundgren JD; Pedersen C; Istituto Superiore di Sanita, Lab. of Virology, Rome, Italy.; OBJECTIVE: To investigate the epidemiology of ADC in Europe through information collected on 6548 subjects who were diagnosed AIDS between Jan. 1980 and Dec. 1989 and who were enrolled in a multicenter European study sponsored by the Commission of the European Communities. We also attempted to assess the role of ZDV t
Progression of nervous system dysfunction in HIV; 5 year follow-up.: Int Conf AIDS. 1994 Aug 7-12;10(1):194 (abstract no. PB0203). Unique Identifier : AIDSLINE ICA10/94369653; Hall CD; Messenheimer J; Wilkins JW; Whaley RA; Robertson WT; Radzion; Hall MJ; Kwock S; Donovan MK; Robertson KR; AIDS Neurologic Center, University of North Carolina, Chapel Hill; 27599.; OBJECTIVE: To assess the degree of nervous system involvement and the rate of progression of disease at different stages of HIV infection. METHODS: A cohort of 133 adults has been followed for up to 5 years with 6 monthly evaluations. Evaluations include: neurologic; psychologic; neuropsychologic; routine and quantita
Quality of life in people with depressive symptoms and HIV.: Int Conf AIDS. 1994 Aug 7-12;10(1):194 (abstract no. PB0205). Unique Identifier : AIDSLINE ICA10/94369654; Rubin HC; Patterson TL; Atkinson JH; Kaplan RM; Koch WL; McCutchan JA; Chandler JL; Grant I; UCSD.; OBJECTIVES: To explore the impact of depressive symptoms on health-related quality of life in people with HIV. METHODS: HIV+ (n = 285) and HIV- (n = 84) men participating in a longitudinal cohort study were administered the Hamilton Depression Index (HAM-D) and the Quality of Well Being Scale (QWB)--a general health-r
Stereotactic brain biopsy in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):194 (abstract no. PB0206). Unique Identifier : AIDSLINE ICA10/94369655; Valadas E; Melo AZ; Ferreira G; Quaresma MJ; Infectious Diseases Department, Hospital de Santa Maria, Lisbon,; Portugal.; One of the most important problems in HIV seropositive patients is the differential diagnosis of possible treatable cerebral mass lesions visualized in CT scan or MRI. The need for a rapid diagnosis is unquestionable; however, the indications and timing for brain biopsy remain controversial. In order to evaluate the e
Hypocholesterolemia and psychological distress in HIV-1 disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):195 (abstract no. PB0208). Unique Identifier : AIDSLINE ICA10/94369656; Shor-Posner G; Feaster D; Blaney N; Miguez-Burbano M; Goodkin K; Elsdorfer C; Baum MK; Department of Epidemiology, University of Miami School of; Medicine, FL.; OBJECTIVE: Altered serum lipid concentrations, (hypertriglyceridemia, hypocholesterolemia) which have been documented in HIV-1 disease, have been associated with behavioral abnormalities. The present study determined the relationship between lipid status and psychosocial behavior in 118 HIV seropositive (CDC Stages II
HIV seroprevalence among psychiatric in-patients at the psychiatric unit of S. Giovanni Hospital, Rome.: Int Conf AIDS. 1994 Aug 7-12;10(1):195 (abstract no. PB0210). Unique Identifier : AIDSLINE ICA10/94369657; Di Genio M; Chiesi A; Pariante F; Falchi Delitala N; D'Aloia D; Degli Esposti M; Mental Health Department, USL RM4, Rome, Italy.; OBJECTIVE: To assess HIV seroprevalence and to identify associated social and psychiatric factors among psychiatric in-patients considered to be at risk of HIV infection. METHODS: Beginning from January 1992, all the in-patients in our unit who were considered to be at risk of HIV infection, were offered to be tested.
Stereotactic brain biopsy of focal intracerebral lesions in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):195 (abstract no. PB0207). Unique Identifier : AIDSLINE ICA10/94369658; Cosin J; Miralles P; Moreno S; Parras M; Salazar F; Bouza E; Infectious Diseases Unit, H.G. Maranon, Madrid, Spain.; OBJECTIVE: To determine the prevalence of different etiologies of intracerebral mass lesions unresponsive to empirical anti-toxoplasma therapy and to evaluate the efficacy and security of stereotactic brain biopsy. PATIENTS AND METHODS: During a period of 16 months, 23 cerebral stereotactic biopsies were performed to
Stressful life events, cortisol and cellular immune changes in asymptomatic, HIV infected individuals.: Int Conf AIDS. 1994 Aug 7-12;10(1):195 (abstract no. PB0209). Unique Identifier : AIDSLINE ICA10/94369659; Petitto J; Lesserman J; Perkins D; Golden J; Folds J; Evans D; Univ. of Florida, Gainesville 32610.; Early HIV infection may produce functional changes in key immunoregulatory cells including T helper cells and macrophages, cells which may harbor the virus, as well as alter the numbers and function of cytotoxic killer cells including NK cells and cytotoxic T cells. We have employed both flow cytometric enumerative an
HIV and the brain: P300 changes do not reflect general subcortical damage.: Int Conf AIDS. 1994 Aug 7-12;10(1):196 (abstract no. PB0214). Unique Identifier : AIDSLINE ICA10/94369660; Katner HP; Moore NC; Tucker KA; Smith MU; Coburn KL; Department of Medicine, Mercer University School of Medicine,; Macon, GA 31208.; OBJECTIVES: Evidence of HIV encephalopathy can be found shortly after seroconversion as a delayed and attenuated P300 auditory event related potential (ERP) component. P300 changes occur before dementia , while the EEG is normal, and increase with successive infection stages (Coburn
Intrathecal synthesis of anti gp120 V3 loop antibodies in HIV-infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):196 (abstract no. PB0213). Unique Identifier : AIDSLINE ICA10/94369661; Mata S; Lolli F; Pinto F; Colao MG; DeMajo E; Mecocci L; Brizzi M; Blanc PL; Paci P; Mazzotta F; Department of Neurological Sciences, University of Florence,; Italy.; OBJECTIVE: The detection of CSF antibody against the V3 loop of HIVMN by peptide ELISA has been recently reported in a high percentage of patients (LUCEY DR et al., J. AIDS 1993, 6:994-1001). We re-evaluated the occurence of intrathecal synthesis (ITS) of antibodies to the V3 loop of HIVMN. METHODS: We measured the an
Prognostic value of conventional EEG-recordings in evaluating HIV-encephalopathy.: Int Conf AIDS. 1994 Aug 7-12;10(1):196 (abstract no. PB0211). Unique Identifier : AIDSLINE ICA10/94369662; Arendt G; Bohn J; Schroerschwarz T; von Giesen HJ; Roick H; Jablonowski H; Department of Neurology, Univ. Dusseldorf, FRG.; OBJECTIVE: HIV-1-related dementia is said to be subcortical and cortical. Whereas the subcortical form presents early in the course of the disease with cognitive and motor signs, the cortical deficits are not very well studied to date. METHODS: 109 neurologically asymptomatic (no c
The role of TNF-alpha in the pathogenesis of vacuolar myelopathy in AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):196 (abstract no. PB0212). Unique Identifier : AIDSLINE ICA10/94369663; Ciardi A; Tan SV; An SF; Guiloff RJ; Scaravilli F; Department of Neuropathology, Institute of Neurology, Queen; Square, London, UK.; The amounts of microglia/macrophages, of cells expressing MHC class II antigen and tumour necrosis factor-alpha (TNF-alpha) were evaluated in the spinal cord. HIV encephalitis/leucoencephalopathy (as shown by the presence of MGCs and/or p24 antigen positive cells) was present in 56% of the cases. Other brain pathology
Cytomegalovirus myelitis mimicking intramedullary spinal cord tumor.: Int Conf AIDS. 1994 Aug 7-12;10(1):197 (abstract no. PB0216). Unique Identifier : AIDSLINE ICA10/94369664; Moulignier A; Mikol J; Gonzalez-Canali G; Wallays C; Thiebaut JB; Dupont B; Hopitaux Tenon, Paris, France.; Cytomegalovirus ( CMV ) induced myelitis is a rare but known complication in AIDS. Presenting with rapidly progressive paraparesis, sensory loss and urine retention, the disease has not been described to our knowledge, as an intramedullary spinal cord tumor. A 33-
CMV polyradiculomyelitis in AIDS: 7 cases.: Int Conf AIDS. 1994 Aug 7-12;10(1):197 (abstract no. PB0217). Unique Identifier : AIDSLINE ICA10/94369665; Jacomet C; Lebrette MG; De Montfort L; el Amrani M; Gozlan J; Girard PM; Rozenbaum W; Hopital Rothschild, Paris, France.; OBJECTIVE: To describe features of CMV polyradiculomyelitis. METHODS: Retrospective chart analysis of 7 cases. RESULTS: Among 861 AIDS patients (pts) from the same unit, 7 cases of CMV polyradiculomyelitis were diagnosed from June 1991 to December 1993. It was t
Is routine ophthalmological study in VIH patients profitable?: Int Conf AIDS. 1994 Aug 7-12;10(1):197 (abstract no. PB0215). Unique Identifier : AIDSLINE ICA10/94369666; Munoz JJ; Artiaga E; Tana P; Carrasco JM; Vidal T; Portilla J; Boix V; Belmonte J; Servicio de Oftalmologia, Hospital General Universitario de; Alicante, Spain.; OBJECTIVE. Evaluation of routine ophthalmological investigations in CDC stage IV HIV + patients admitted to the Infections Diseases Unit of our hospital, without ophthalmological signs or symptoms. To show the relationship between the ocular pathology found and the cause of hospital admission. MATERIAL AND METHODS. Be
Risk factors for retinal detachment in patients with CMV-retinitis.: Int Conf AIDS. 1994 Aug 7-12;10(1):197 (abstract no. PB0218). Unique Identifier : AIDSLINE ICA10/94369667; Geier SA; Sadri I; Bogner JR; Klauss V; Goebel FD; Medizinische Poliklinik LMU, Munich, FRG.; OBJECTIVE: The purpose of this study was to investigate risk factors associated with retinal detachment in patients with AIDS and CMV-retinitis. PATIENTS AND METHODS: 69 patients with CMV-retinits were evaluated with respect to peripheral involvement of the retina. Myopia was evaluated as an additional not CMV-retinit
Laser photocoagulation in AIDS patients with CMV retinitis.: Int Conf AIDS. 1994 Aug 7-12;10(1):198 (abstract no. PB0219). Unique Identifier : AIDSLINE ICA10/94369668; Kosmides P; Ladas JD; Dadoush G; Nicolaidou S; Koulios P; Vergados J; Liarikos S; St. Savvas Hospital Eye Clinic, Athens, Greece.; OBJECTIVE: In our multicenter study 61 patients 89 Eyes with active disease or scarred CMV retinitis were studied. We studied the rate of retinal detachment and the role of Laser photocoagulation in CMV retinitis. METHODS: Ten 10 patients who developed retinal d
Maintenance therapy of cytomegalovirus retinitis with intravitreal injection only: safety and effectiveness.: Int Conf AIDS. 1994 Aug 7-12;10(1):198 (abstract no. PB0220). Unique Identifier : AIDSLINE ICA10/94369669; Pastor M; Viciana P; Calvo R; Nuno E; Seva E; Rodriguez MJ; Ophtalmology Departament, Hospital V. Ricio, Seville, Spain.; OBJECTIVE: To determine the safety and effectiveness of maintenance therapy of cytomegalovirus ( CMV ) retinitis with intravitreal injections, without intravenous maintenance therapy in AIDS patients. METHODS: After complicatio
Ocular manifestations in HIV patients in Japan.: Int Conf AIDS. 1994 Aug 7-12;10(1):198 (abstract no. PB0221). Unique Identifier : AIDSLINE ICA10/94369670; Nagata Y; Fujino Y; Ono A; Mochizuki M; Oka S; Kimura S; Shimada K; Dept. of Ophthalmol., Univ. of Tokyo, Japan.; OBJECTIVE: Ocular disorders have been noted in patients infected with HIV and are one of the most serious complications in AIDS patients. However, the ocular features of HIV-seropositive patients have not been well documented in Japan , we investigated the ocular manifestations in 70e
Predictive factors of sequelae in AIDS patients with brain toxoplasmosis.: Int Conf AIDS. 1994 Aug 7-12;10(1):198 (abstract no. PB0222). Unique Identifier : AIDSLINE ICA10/94369671; Perez-Cecilia E; Sanchez J; Jimenez-Escrig A; Roca V; Romero J; Palau E; Picazo J; Hospital Clinico, Madrid, Spain.; OBJECTIVE: To determine predictive factors of sequelae, in AIDS patients (pts) with brain toxoplasmosis (BT), properly treated. METHODS: 33 pts diagnosed of BT were included. Diagnosis was based in clinical and imaging criteria and a positive response to antitoxoplasma therapy (91%), or a pathological proven disease (e
AIDS dementia complex: a study of 20 patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):199 (abstract no. PB0225). Unique Identifier : AIDSLINE ICA10/94369672; Stavrianeas N; Kivellos S; Retzos M; Samartzis D; Chatzivasiliou M; Stratigos J; Unit of specific infectious diseases, A. Sygros Hospital, Athens,; Greece.; OBJECTIVES: Symptoms and signs of AIDS-Dementia Complex and the the way they appear. METHODS: Twenty patients hospitalized in A. Sygros hospital with a diagnosis of AIDS-Dementia have been studied. The clinical & neurological examination, the neuropsychological evaluation, the blood & CSF serological tests, the
HIV associated dementia: frequency and differentiating factors compared to other neurological complications of HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):199 (abstract no. PB0224). Unique Identifier : AIDSLINE ICA10/94369673; Sanchez-Portocarrero J; Jimenez-Escrig A; Ayuso J; Perez-Cecilia E; Roca V; Barquero M; Varela de Seijas E; Hospital Clinico, Madrid, Spain.; OBJECTIVES: Define the frequency of appearance of HIV associated dementia (HAD) and establish the differentiating factors which may exist when it is compared to other HIV associated neurological disorders (HAND). METHOD: During a period of four years (1989-1992) we studied prospecto
Neuropsychological and neurodiagnostic by imaging (MRI-SPECT) early signs of AIDS dementia complex (ADC) in asymptomatic HIV-1 infected subjects: follow up.: Int Conf AIDS. 1994 Aug 7-12;10(1):199 (abstract no. PB0226). Unique Identifier : AIDSLINE ICA10/94369674; Rosci MA; Pigorini F; Bernabei A; Pau FM; Merigliano DE; Leonetti C; L. Spallanzani Hospital for Infectious Diseases, Rome, Italy.; The objective of our study was to define the evolution of the early signs of AIDS Dementia Complex, shown by neuropsychological tests, SPECT (Single Photon Emission Computed Tomography) and MRI, at base line observation (AIDS, Volume 6 No. 11-1992), in asymptomatic HIV-1 infected subjects. We also aimed at defining tho
Outbreak of CNS toxoplasmosis due to AIDS in Bombay.: Int Conf AIDS. 1994 Aug 7-12;10(1):199 (abstract no. PB0223). Unique Identifier : AIDSLINE ICA10/94369675; Lanjewar DN; Dept. of Pathology, Grant Medical College, Bombay, India.; OBJECTIVE: To determine the incidence of CNS opportunistic infection among patients with AIDS. METHODS: Forty cases of AIDS were included in autopsy study. Examination of brain was carried out by H & E stain. Special stains were carried out for tubercle bacilli, fungal infection and myelin fibres. RESULTS: Morpholo
Effect of maternal HIV status on infant growth & survival.: Int Conf AIDS. 1994 Aug 7-12;10(1):20 (abstract no. 054B). Unique Identifier : AIDSLINE ICA10/94369676; Boulos R; Ruff A; Coberly J; McBrien M; Halsey JD; CDS, Port-au-Prince, Haiti.; OBJECTIVE: Although growth failure and increased mortality are common among HIV-infected infants, less is known about uninfected infants born to HIV-1 seropositive women. This study was undertaken to compare the growth and survival of breastfed infants born to HIV-1 seropositive and seronegative women. METHODS: Growth
Glutathione deficiency in the liver of HIV seropositive subjects.: Int Conf AIDS. 1994 Aug 7-12;10(1):20 (abstract no. 052B). Unique Identifier : AIDSLINE ICA10/94369677; Barbarini G; Filice C; Zanaboni D; Garavelli G; Malfitano A; Brunetti E; Bellomo G; Dept. of Infectious Diseases IRCCS S. Matteo, Italy.; OBJECTIVE: Reduced-Glutathione (GSH) is decreased in plasma, epithelial-lung fluid and T lymphocytes in HIV+ patients. Because this deficiency may potentiate HIV replication and accelerate disease progression, we determined the level of this Tripeptide in the liver, to know if its concentration is correlated either to
Pediatric AIDS in Sao Paulo-Brazil = 8 years later.: Int Conf AIDS. 1994 Aug 7-12;10(1):20 (abstract no. 053B). Unique Identifier : AIDSLINE ICA10/94369678; Queiroz W; Della Negra M; Yu CL; Paste AA; Soraggi Neto C; Silva LM; Vazquez LM; Inst. Infectol. Emillio Ribas, Sao Paulo, SP, Brazil.; OBJECTIVES: To show the clinical and epidemiological features of HIV infected children followed in a specialized service in Sao Paulo, Brazil . METHODS: Since 1986 419 children up to 13 years of age were followed in our center, by the same medical team. We analyzed retrospectively t
Randomized comparative trial of ZDV+ddI vs ZDV+ddC on short-term safety and laboratory markers in subjects with CD4s of 50 to 350/microL. Canadian HIV Trials Network.: Int Conf AIDS. 1994 Aug 7-12;10(1):20 (abstract no. 055B). Unique Identifier : AIDSLINE ICA10/94369679; Montaner JS; Srour L; Singer J; Cassol S; O'Shaughnessy MV; Schechter MT; Bristol-Myers & Squibb, Canada.; OBJECTIVE: To compare the short term safety and surrogate marker effects of ZDV/ddI vs. ZDV/ ddC . METHODS: HIV+ patients tolerant of ZDV > or = 400 mg/d were eligible if they were ddI and ddC naive and had CD4 counts of 50 to 350/mm3. Eligible patients
Clinical evaluation of a HIV+ patient with Guillain-Barre syndrome (GBS).: Int Conf AIDS. 1994 Aug 7-12;10(1):200 (abstract no. PB0227). Unique Identifier : AIDSLINE ICA10/94369680; Watanabe R; Iino M; Tachikawa H; Honda M; Sagara H; Yokohama City Hospital, Japan.; OBJECTIVE: a) To evaluate the clinical course and treatment of the first Japanese HIV+ patient with GBS. b) To investigate the immunological background of the patient. METHODS: Regular neurological examination was carried out to assess both the clinical course and the effect of the treatment, especially of
Persistent neutrophilic meningitis in patients with AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):200 (abstract no. PB0230). Unique Identifier : AIDSLINE ICA10/94369681; Roca V; Sanchez-Portocarrero J; Martin T; Perez-Cecilia E; de la Torre F; Picazo JJ; Fernandez-Cruz A; Hospital Universitario San Carlos, Madrid, Spain.; We report five cases of Persistent Neutrophilic Meningitis (PNM), diagnosed in base of case-definition criteria proposed by J. E. Peacock (persistent neutrophilic pleocytosis in CSF for more than 7 days, despite to be treated with appropriate antimicrobial regime), and accounted in patients with HIV-infection. In 4 th
Treatment with intravenous immunoglobulin in severe Guillain-Barre syndrome and HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):200 (abstract no. PB0228). Unique Identifier : AIDSLINE ICA10/94369682; Rodriguez Arrondo F; Huarte I; Von Wichmann MA; Iribarren JA; Arrizabalaga J; Urtasun M; Infectious Diseases Unit, Hospital Ntra Sra Aranzazu, San; Sebastian, Spain.; OBJECTIVE: To determine the Efficacy and safety of High-dose intravenous immunoglobulin in the treatment of severe Guillain-Barre syndrome (GBS) in patients with HIV infection. METHODS: Two severely affected patients were treated with five doses of a preparation of immunoglobulin (0.4 g per kilogram daily). Treatment
Xerophthalmia in HIV-disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):200 (abstract no. PB0229). Unique Identifier : AIDSLINE ICA10/94369683; Libera S; Geier SA; Klauss V; Goebel FD; Medizinische Poliklinik and Augenklinik der LMU, Munich, Germany.; OBJECTIVE: The purpose of this study was to look for an association of severity of HIV disease with decreased tear production in a controlled setting. PATIENTS AND METHODS: 72 patients (144 eyes) and 20 age- and sex-matched controls (40 eyes) were prospectively studies. Tear production was measured applying the Schirm
Delayed PML diagnosis and d4T treatment.: Int Conf AIDS. 1994 Aug 7-12;10(1):201 (abstract no. PB0234). Unique Identifier : AIDSLINE ICA10/94369684; Lutz B; Mogabgab W; Santos M; Combs K; New Orleans Health Department, Owen Home Infusion, La.; OBJECTIVE: To describe the clinical course of progressive multifocal leukoencephalopathy (PML) in a patient taking stavudine ( d4T ). METHODS: Single patient case report. RESULTS: After
Electrophysiological evaluation of 2',3',-dideoxycytidine (ddC) caused polyneuropathy. A follow-up study.: Int Conf AIDS. 1994 Aug 7-12;10(1):201 (abstract no. PB0232). Unique Identifier : AIDSLINE ICA10/94369685; Roick H; von Giesen HJ; Jablonowski H; Arendt G; Department of Neurology & Medicine, Heinrich-Heine-University,; Duesseldorf, FRG.; 18 HIV-seropositive individuals (5 CDC II, 2 CDC III, 11 CDC IV; age 41.97 +/- 10.41 years) were followed-up by nerve conduction velocity studies and clinical examination before and during an antiretroviral therapy with 2 ,3 ,-dideoxycytidine ( ddC ) (3
Pilot study of zidovudine (AZT) and zalcitabine (ddC) combination in HIV-associated dementia.: Int Conf AIDS. 1994 Aug 7-12;10(1):201 (abstract no. PB0233). Unique Identifier : AIDSLINE ICA10/94369686; McIntyre K; Torres R; Luck D; Tavalouras G; St. Vincent's Hospital and Medical Center of New York, N.Y.; OBJECTIVE: To evaluate the safety and efficacy of zidovudine ( AZT ) and zalcitabine ( ddC ) combination therapy in patients with HIV-ass
Treatment of AIDS and progressive multifocal leucoencephalopathy (PML) with cytarabine when the brain biopsy is unsuccessful or impossible.: Int Conf AIDS. 1994 Aug 7-12;10(1):201 (abstract no. PB0231). Unique Identifier : AIDSLINE ICA10/94369687; Ravaux I; Quinson AM; North MO; Gallais H; Infectious Diseases Unit, Hopital La Conception, Marseille,; France.; OBJECTIVE: Is it justified to propose a treatment with CYTARABINE IV when the diagnosis is only strongly presumed on clinical courses. CT scanner and IRM, therapeutic criteria and responsiveness to a three weeks course of antitoxoplasma therapy. OUR EXPERIENCE: We have studied 12 AIDS patients, 7 women and 5 men, from
A survey of adult meningitis in Zimbabwe.: Int Conf AIDS. 1994 Aug 7-12;10(1):202 (abstract no. PB0236). Unique Identifier : AIDSLINE ICA10/94369688; Malin A; Bwakura T; Robertson V; Musvaire P; University of Zimbabwe Medical School, Harare.; OBJECTIVE: (1) To survey all types of adult meningitis. (2) To identify clinical and laboratory markers which distinguish types of meningitis as an aid to diagnosis in developing countries. METHODS: Blood, CSF (cerebrospinal fluid) and clinical data were collected prospectively from unselected, consecutive adults admi
HIV-1/HTLV-1 coinfection is associated with a high frequency of myelopathies.: Int Conf AIDS. 1994 Aug 7-12;10(1):202 (abstract no. PB0237). Unique Identifier : AIDSLINE ICA10/94369689; Harrison LH; Schechter M; Vaz BP; McArthur JC; Johns Hopkins University.; INTRODUCTION: Although in postmortem examinations myelopathies are found in up to 30% of HIV-1 infected patients, their clinical correlates are recognized far less frequently. How often myelopathies occur among subjects with HTLV-1 and HIV coinfection is not known. OBJECTIVE: To compare the frequency of clinically det
Magnesium level and peripheral neuropathy.: Int Conf AIDS. 1994 Aug 7-12;10(1):202 (abstract no. PB0235). Unique Identifier : AIDSLINE ICA10/94369690; Stroud S; Salvato P; Thompson C; Med. Univ., Charleston, SC.; OBJECTIVE: To determine if a relationship exists between low serum magnesium (Mg) levels and symptoms characteristic of peripheral neuropathy . METHODS: 68 pts. with HIV infection (59 AIDS) provided data. Histories were compatible with sensory dysfunction. Physical exa
Panavir: a novel therapy for HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):202 (abstract no. PB0238). Unique Identifier : AIDSLINE ICA10/94369691; Hendler SS; Sanchez RA; Nagourney RA; Lauermann MW; Sonne M; Strayer G; Vyrex Corp., La Jolla, CA 92037.; OBJECTIVE: To assess a) the in-vitro activity of Panavir (4,4 -isopropylidenedithio-bis-2,6-di-t-butylphenol; a specially-formulated lipophilic antioxidant) against HIV replication and against activation of latently infected cells, and b) the clinical safety, pharmacokinetics, and efficacy in the treatment of HIV dise
Anti-HIV flavonoids from Morus alba L.: Int Conf AIDS. 1994 Aug 7-12;10(1):203 (abstract no. PB0240). Unique Identifier : AIDSLINE ICA10/94369692; Luo SD; Nemec J; Ning BM; Li QX; Kunming Institute of Botany, China.; San Baipi. root of Morus alba L. (Moraceae) is a traditional Chinese medicine for cough, asthma and other diseases. The alcohol extract of San Baipi displayed activity against HIV in vitro. Solvent partition, fractionation on CC and preparing TLC provided three anti-HIV flavonoids: morusin, kuwanon H and morusin-4 -gl
Loviride induces a prolonged rise of the CD4 count in asymptomatic HIV-1+ subjects.: Int Conf AIDS. 1994 Aug 7-12;10(1):203 (abstract no. PB0242). Unique Identifier : AIDSLINE ICA10/94369693; De Brabander M; Staszewski S; De Cree J; Verhaegen H; Stoffels P; Van Den Broeck R; Roels V; Janssen PA; CRU St. Bartholomeus, Merksem, Belgium.; OBJECTIVE: to compare the effect on CD4 count of treatment with placebo or with the alpha-APA compounds R18893 and loviride (R89439) a new derivative with improved activity and selectivity and greatly enhanced bioavailability (plasma levels > 100x the IC50 for HIV-1) METHODS: double blind, double dummy investigation o
Phase I/II trial of oral Panavir for HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):203 (abstract no. PB0239). Unique Identifier : AIDSLINE ICA10/94369694; Nagourney RA; Hendler SS; Sanchez RA; Strayer G; Sonne M; Lauermann MW; Long Beach Memorial Medical Center, CA 90801-1428.; OBJECTIVE: To determine safety, efficacy and bioavailability of Panavir (4,4 -isopropylidenedithio-bis-2,6-di-t-butylphenol, specially formulated) as an oral HIV drug. METHODS: From July 1992 to February 1994, 25 HIV+ adults received daily oral Panavir at 200 mg (n = 6), 400 mg (n = 7) and 800 mg (n = 12). Age range 2
Vesnarinone; a preliminary therapeutic approach in HIV-1 infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):203 (abstract no. PB0241). Unique Identifier : AIDSLINE ICA10/94369695; Maruyama Y; Kobayashi N; Maruyama I; Osame M; Third Department of Internal Medicine, School of Medicine,; Kagoshima University, Japan.; OBJECTIVE: To investigate the efficacy and safety of a new anti-retroviral agent, vesnarinone. METHODS: Studied were nine patients with HIV-1 infected hemophiliacs (30.3 +/- 5.6 years old, male, AIDS 5, ARC 3, AC 1). Eight patients received AZT and/or
Phase I/II study of curdlan sulfate--an HIV inhibitor.: Int Conf AIDS. 1994 Aug 7-12;10(1):204 (abstract no. PB0244). Unique Identifier : AIDSLINE ICA10/94369696; Gordon M; Guralnik M; Lang W; Baker M; Goodgame J; DeMarzo C; Mimura T; Kaneko Y; AJI PHARMA USA, Inc., Glenpointe Centre West, Teaneck, NJ 07666.; OBJECTIVE: To assess the safety, tolerance and pharmacokinetics of curdlan sulfate (CRDS), a semisynthetic beta-glucan, in HIV-positive individuals who have CD4 T-lymphocytes below 500 cells/cmm, administered as single doses over 30 minutes, or four hours, or daily for seven days. METHODS: CRDS was administered in sep
Todoxin in AIDS. Phase 3 controlled multinational cooperative open study. Todoxin Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):204 (abstract no. PB0243). Unique Identifier : AIDSLINE ICA10/94369697; Radojicic C; Jovanovic T; Petrovic S; OBJECTIVE: To determine the efficacy of TODOXIN (see also Track A14 and D17/D18) to delay the onset of AIDS. METHODS: 20 HIV+ patients (pts) were stratified into 2 groups at study entrance. (G1): CD4 or = 300/4 IVDU, 2 HEM, 2 HS/sero+ 1985-91,
Treatment of HIV-infected patients with advanced symptomatic disease with WF10 solution (TCDO).: Int Conf AIDS. 1994 Aug 7-12;10(1):204 (abstract no. PB0245). Unique Identifier : AIDSLINE ICA10/94369698; Busch HW; Christensen S; Reichelt D; Jahn S; Zidek W; Medizinische Poliklinik, WWU Munster, Germany.; OBJECTIVE: Since the chlorite-oxygen reaction product (TCDO) activates cellular phagocytosis and killer systems such as monocytes-macrophages, NK-cells and cytotoxic T-lymphocytes and has also been shown to exhibit significant virucidal activity against HIV-particles, our aim was to determine the clinical effect of WF
Use of N-(4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]-1- glutamic acid (methotrexate) (MT) in inhibiting replication of human immunodeficiency virus-1(HIV-1) in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):204 (abstract no. PB0246). Unique Identifier : AIDSLINE ICA10/94369699; Kumar S; Clinical Regional Laboratory, Flossmoor, IL 60422.; OBJECTIVE: To study the possible effects of different drugs on the activity and growth and on controlling, delaying, or preventing the replication of the AIDS virus (HIV-1) in humans, we have used peripheral blood lymphocytes from untreated AIDS-positive patients and studied the effect of MT, a drug used for the treat
Association zidovudine and dipyridamole: a pharmacokinetic study.: Int Conf AIDS. 1994 Aug 7-12;10(1):205 (abstract no. PB0250). Unique Identifier : AIDSLINE ICA10/94369700; Livrozet JM; Schrive I; Brunel F; Fournier F; Plasse JC; Touraine JL; Hopital E. Herriot, Lyon, France.; OBJECTIVE: Some studies reported dipyridamole potentiates antiviral effect of zidovudine (ZDV) by inhibition of thymidine uptake and phosphorylation. The aim of this study was to evaluate the effect of the association ZDV and dipyridamole in HIV seropositive patients on ZDV pharmacokinetic parameters (intracellular an
Nimodipine+ZDV vs ZDV in patients with ADC.: Int Conf AIDS. 1994 Aug 7-12;10(1):205 (abstract no. PB0248). Unique Identifier : AIDSLINE ICA10/94369701; Galgani S; Narciso P; Balestra P; Pigorini F; Pau F; Sette P; Tozzi V; Alba L; Grisetti S; Visco G; USLRM10, Rome, Italy.; OBJECTIVE. To evaluate the activity of ZDV+Nimodipine (NMP) (an oral calcium-channel blocking agent that may reduce HIV-induced neurotoxicity in vitro) association in the treatment of HIV-induced cognitive dysfunctions. METHODS. Design: open, case control study. Eligibility criteria:CD4 cells
Trials of elaboration more universal AIDS therapy: anti-HIV laser traps for blood, virucidal hyperbaric gas, tissue pH modifiers.: Int Conf AIDS. 1994 Aug 7-12;10(1):205 (abstract no. PB0249). Unique Identifier : AIDSLINE ICA10/94369702; Vasilionkaitis V; Research laboratory of Clinical Biomechanics & Modern Treatment; Methods Vilnius, Lithuania.; OBJECTIVES: Biomedical engineering approaches to prevention and treatment of AIDS or infected with HIV was investigated. Lack of many anti-HIV/AIDS curative procedures depend on impossibility to reach inactivation all of HIV with following renew of infection. A necessity of elaboration of more universal anti-HIV/AIDS
Two cases of AIDS associated with hemophilia A, treated effectively by the concomitant use of SNMC and ddI.: Int Conf AIDS. 1994 Aug 7-12;10(1):205 (abstract no. PB0251). Unique Identifier : AIDSLINE ICA10/94369703; Mori K; Sendai Hematologic Clinic, Japan.; OBJECTIVE: We studied a combination therapy using glycyrrhizin (Stronger Neo-Minophagen C: SNMC) which has been shown to be effective against HIV at an ultra-high dose by a different mechanism from that of
Antiretroviral therapy effect on CD4+ cell counts. A way to evaluate drug efficacy.: Int Conf AIDS. 1994 Aug 7-12;10(1):206 (abstract no. PB0254). Unique Identifier : AIDSLINE ICA10/94369704; D'Offizi G; Ferrara R; Pinter E; Rosso R; Prozzo A; Pontesilli O; Aiuti F; Department of Clin. Immunology and Allergology, Univ. of Rome La; Sapienza, Italy.; OBJECTIVE: CD4+ lymphocyte count is a very important surrogate marker to evaluate antiretroviral drug efficacy. In this study we tested drug efficacy on CD4+ cell number calculating the slope of the linear regression line for CD4+ cell during zidovudine and didanosine therapy. METHODS: We retrospectively calculated th
Effect of Concorde Study release on anti-retroviral (AR) use in community programs for clinical research on AIDS (CPCRA) trials.: Int Conf AIDS. 1994 Aug 7-12;10(1):206 (abstract no. PB0253). Unique Identifier : AIDSLINE ICA10/94369705; Ward TT; Sampson J; Wentworth D; Wallace JA; Portland OR VA Med Ctr.; OBJECTIVE: Widespread attention was given to two studies of AR therapy at the Berlin International AIDS meetings in mid-June 1993: the Concorde Study of AZT Monotherapy (mono) and the ACTG 175 study of combination (combo) AZT and ddI. We sought to det
Efficacy of ZDV/DDC measured by surrogate markers.: Int Conf AIDS. 1994 Aug 7-12;10(1):206 (abstract no. PB0255). Unique Identifier : AIDSLINE ICA10/94369706; Banhegyi D; Ujhelyi E; Gerlei Z; Fust G; Saint Laszlo Hospital Dept. of Immunology, Budapest, Hungary.; OBJECTIVES: In a pilot study of the multicenter safety and tolerance study (M50002) the efficacy of ZDV/DDC (zidovudine/dideoxycytidine) combination were investigated by means of clinical signs and surrogate markers. METHODS: 15 patients (pts) who had been treated with ZDV (mean 24.5 months; 16-40) and had disease pro
The interim safety results and pharmacokinetics of a combination of R89439 and zidovudine therapy in HIV-infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):206 (abstract no. PB0252). Unique Identifier : AIDSLINE ICA10/94369707; Colebunders R; Vandenbruaene M; Delescluse J; Vander Endt D; De Cree J; De Brabander M; Peeters M; Van den Broeck R; Stoffels P; Tropical Institute of Antwerp, Belgium.; R89439 (loviride) is the most potent product of the alpha-APA series (A group of Non Nucleoside Reverse Transcriptase Inhibitors) with an IC50 of 1.7 ng/ml and good oral bioavailability (Cp at 8h = 130 ng/ml). OBJECTIVES: The objectives of the present trial were to document the possible pharmacokinetic interactions be
Didanosine (DDI) vs zidovudine (ZDV) in HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):207 (abstract no. PB0256). Unique Identifier : AIDSLINE ICA10/94369708; Milini P; Casari S; Donisi A; Gregis G; Tomasoni D; Bergamasco A; Carosi G; Inst. of Infectious and Tropical Dis., Univ. of Brescia, Italy.; OBJECTIVE: To value efficacy and tollerability of DDI therapy versus ZDV in patients with HIV infection. METHODS: A case-control, randomized, open study. Patients (pts): ARC subjects (CDC 87) with CD4+ cell count
Effects of combined treatment with zidovudine and dideoxyinosine in 104 HIV+ patients with prior zidovudine therapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):207 (abstract no. PB0258). Unique Identifier : AIDSLINE ICA10/94369709; de Saint Martin L; Pialoux G; Tuppin P; Gonzalez-Canali G; Feuillie V; Dupont B; Institut Pasteur Hospital, Paris, France.; OBJECTIVES: 104 HIV infected patients with infection progressing in spite of ongoing AZT were enrolled into a longitudinal retrospective study of safety and impact on disease course of combined therapy with zidovudine (AZT) and dideoxyinosine (ddI). M
Open label combination of zidovudine (ZVD) plus didanosine (DDI) for the treatment of patients with HIV infection and absolute CD4 lymphocytes counts > 100 < 500 mega/l.: Int Conf AIDS. 1994 Aug 7-12;10(1):207 (abstract no. PB0257). Unique Identifier : AIDSLINE ICA10/94369710; Suleiman G; Suleiman J; Suleiman S; Castro I; Ferreira Junior O; Instituto de Infectologia Emilio Ribas, Sao Paulo, Brazil.; In this ongoing study, 34 patients with HIV infection and CD4 counts > 100
Toxicity and carcinogenicity of dideoxycytidine in mice.: Int Conf AIDS. 1994 Aug 7-12;10(1):207 (abstract no. PB0259). Unique Identifier : AIDSLINE ICA10/94369711; Rao G; Sanders V; Elwell M; Giles H; Heath J; Lindamood C 3rd; NIEHS, NIH, Res. Tri. Park, NC 27709.; OBJECTIVE: The 2 ,3 -dideoxycytidine ( ddC ) is an approved nucleoside for treatment of HIV positive patients. The purpose of the project is to evaluate the toxic and carcinogenic potential of ddC by short-term studies in two different mouse models. MET
AZT monotherapy compared to AZT+DDC combined antiviral therapy and AZT+DDC+NAC (100 patients each group).: Int Conf AIDS. 1994 Aug 7-12;10(1):208 (abstract no. PB0260). Unique Identifier : AIDSLINE ICA10/94369712; Feregrino-Goyos M; Eid Lidt G; Gallegos Perez H; Alvarado DR; Mino LD; Clinica ISI de Norteamerica, Mexico D.F., Mexico.; OBJECTIVES: Comparison of the clinical response to three different antiviral schematic in HIV patients with less than 500 CD4 (Stages II, III, and IV del CDC). METHODS: During one year, 300 HIV patients with less than 500 CD4 were studied. Group I 100 patients received daily 500 mg of
Efficacy and toxicity of DDI and AZT monotherapy versus combined and alternating AZT/DDI regimens.: Int Conf AIDS. 1994 Aug 7-12;10(1):208 (abstract no. PB0261). Unique Identifier : AIDSLINE ICA10/94369713; Schlenzig C; Poppinger J; Wolf E; Jaeger H; KIS--Curatorium for Immunedeficiency, Munich, Germany.; OBJECTIVE: To compare the efficacy and toxicity of single agent ddI and AZT therapy versus combined and alternating AZT/ddI regimens in 100 AZT and ddI virgin patients with CD4+ counts 50-300 cells/ml. METHODS: At time of interim analysis 50 HIV AB po
Influence of antiretroviral treatment on the AIDS patients survival--a private practice experience.: Int Conf AIDS. 1994 Aug 7-12;10(1):208 (abstract no. PB0263). Unique Identifier : AIDSLINE ICA10/94369714; Del Bianco R; Ministerio da Saude, Sao Paulo, Brazil.; OBJECTIVE: To evaluate the relationship between epidemiological and clinical data with the survival of AIDS patients. METHODS: Retrospective study of 40 adult patients with AIDS seen in the private practice since 1985 until their death. Age of infection, sex, risk behaviour, CD4 cell count were evaluated as well as th
Study of 31 acute HIV-1 infection (API).: Int Conf AIDS. 1994 Aug 7-12;10(1):208 (abstract no. PB0262). Unique Identifier : AIDSLINE ICA10/94369715; Sinicco A; Biglino A; Gioannini P; Inst. Infect. Diseases, Univ. of Turin, Italy.; We directly observed 31 API and 168 asymptomatic seroconversions (AS) in patients (pts) who were seronegative in the previous 12 months. API was more frequently seen in pts who had HIV-1 through sex (chi 2 = 29.1; p = .00000). Api clinical aspects ranged from flu-like syndrome to mononucleosis-like syndrome. Two pts h
Effect of rate of CD4 decline on the efficacy of early ZDV therapy in asymptomatic HIV infection.: Int Conf AIDS. 1994 Aug 7-12;10(1):209 (abstract no. PB0265). Unique Identifier : AIDSLINE ICA10/94369716; Easterbrook PJ; Yu LM; Babiker A; Gazzard BG; Boag F; McLean K; Chelsea and Westminster Hospital, London, U.K.; OBJECTIVE: To determine whether asymptomatic HIV infected patients with rapid CD4 decline are a subgroup who might benefit most from early ZDV therapy. METHODS: Analysis of data on 197 patients from 3 centres participating in the Concorde trial: a randomized double-blind comparative trial of ZDV (1g/day) vs. placebo (
Non-nucleoside RT inhibitors with structural diversity but potent anti-HIV activity.: Int Conf AIDS. 1994 Aug 7-12;10(1):209 (abstract no. PB0264). Unique Identifier : AIDSLINE ICA10/94369717; Yang SS; Buckheit RW Jr; Bader JP; DTP, NCI, Bethesda, MD 20892.; Current thrust in controlling AIDS targets the infection and replication of the human immunodeficiency virus (HIV). To date treatment of AIDS has relied upon nucleoside reverse transcriptase (RT) inhibitors such as AZT , ddI,
Retrospective study of zidovudine (ZDV) or didanosine (ddI) monotherapy or zalcitabine plus zidovudine (ddC + ZDV) combination therapy in patients with early AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):209 (abstract no. PB0266). Unique Identifier : AIDSLINE ICA10/94369718; Barr M; Torres RA; St. Vincent's Hospital and Medical Center, NYC.; OBJECTIVE: To assess the relative efficacies of zidovudine (ZDV) and didanosine (ddI) monotherapies compared to combination therapy with zalcitabine ( ddC ) plus zidovudine (ZDV) in 150 patients with ear
Triple combination therapy of Foscarnet.: Int Conf AIDS. 1994 Aug 7-12;10(1):209 (abstract no. PB0267). Unique Identifier : AIDSLINE ICA10/94369719; Convey P; Scolaro M; Brucker R; OBJECTIVE: To evaluate the efficacy of Foscarnet, a non-nucleoside HIV-1 reverse transcriptase inhibitor, given in combination with 2 nucleoside HIV reverse transcriptase inhibitors on immunological and virological markers. METHOD. Foscarnet was given intravenously (90 mg/kg every 12 hours as an induction for 30 days,
Comparative study of Ro 31-8959 and zidovudine (ZDV) vs. ZDV and zalcitabine (ddC) vs. Ro 31-8959, ZDV, and ddC. The ACTG 229 Protocol Team.: Int Conf AIDS. 1994 Aug 7-12;10(1):21 (abstract no. 058B). Unique Identifier : AIDSLINE ICA10/94369720; Collier AC; Coombs RW; Timpone J; Schoenfeld DA; Bassett R; Baruch A; Corey L; University of Washington ACTU, Harborview Medical Center, Seattle; 98104-2499.; OBJECTIVE: To evaluate the safety, tolerance, and antiviral efficacy of the HIV proteinase inhibitor Ro 31-8959 and ZDV vs. ZDV and ddC vs. Ro 31-8959, ZDV and ddC. METHODS: The study was a phase II, multicenter, double-blind, randomized, placebo-cont
HIV-1 viremia changes and development of drug-related viral mutations in patients (pts) receiving long-term combination therapy with AZT/ddI.: Int Conf AIDS. 1994 Aug 7-12;10(1):21 (abstract no. 056B). Unique Identifier : AIDSLINE ICA10/94369721; Kojima E; Shirasaka T; Anderson BD; Chokekijchal S; Steinberg S; Broder S; Yarchoan R; Mitsuya H; National Cancer Institute, Bethesda, MD.; OBJECTIVE: To investigate viremia changes and the development of drug-related viral mutations in pts with advanced HIV-1 infection receiving AZT and ddl in combination. METHODS: HIV-1 virion numbers in serum (VPs) from a subset of 26 pts randomized to
Safety and efficacy of ZDV addition to 3TC monotherapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):21 (abstract no. 057B). Unique Identifier : AIDSLINE ICA10/94369722; van Leeuwen R; Boucher C; Reiss P; Schuurman R; Nijhuis M; Danner S; National AIDS Therapy Evaluation Centre, University of Amsterdam,; The Netherlands.; OBJECTIVE: Lamivudine ( 3TC , (-) enantiomer of 2 -3 -thiacytidine) and zidovudine (zdv) exhibit in vitro synergistic antiviral activity. Moreover, an RT mutation encoding for 3TC resis
Zidovudine & alpha-interferon vs zidovudine alone in HIV infected persons with CD4+ cell counts of 150-500/mm3--results of the 'ZIDON' trial.: Int Conf AIDS. 1994 Aug 7-12;10(1):21 (abstract no. 059B). Unique Identifier : AIDSLINE ICA10/94369723; Fernandez-Cruz E; Lang JM; Frissen PH; Chateauvert M; Furner V; Boucher CA; Dowd P; Stevens J; OBJECTIVE: To evaluate the efficacy and safety of zidovudine (ZDV) and lymphoblastoid interferon-alpha (IFN-alpha) combination therapy compared with ZDV monotherapy in HIV infected subjects with CD4+ T cell counts between 150 and 500/mm3. METHODS: Patients who had neve
Comparative evaluation of ZDV and ddI in AIDS patients with previous ZDV treatment. A randomized multicenter study ISS-901. Italian AZT/DDI Evaluation Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):210 (abstract no. PB0271). Unique Identifier : AIDSLINE ICA10/94369724; Tomino C; Floridia M; Chiesi A; Dally LG; Seeber A; Fragola V; Sebastiani G; Vella S; Lab. of Virology, Istituto Superiore di Sanita, Rome.; OBJECTIVES: To evaluate in patients with AIDS the possible benefit of switching to didanosine after 6-18 months of AZT treatment in terms of survival and onset of new AIDS-defining diseases. METHODS: Multicenter, open, randomized study. AIDS patients
Design and implementation of a community based trial for the comparison of combination ZDV with ddC or ddI to ZDV monotherapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):210 (abstract no. PB0269). Unique Identifier : AIDSLINE ICA10/94369725; Saravolatz L; Winslow D; Stein D; Dehlinger M; Collins G; Deyton L; Henry Ford Hospital, Detroit, MI 48202.; OBJECTIVE: This study compares ZDV alone with ZDV given in combination with either ddI or ddC with respect to efficacy and safety in HIV infected patients with CD4 counts of
Preliminary results of a phase II study on the efficacy and tolerability of AZT plus alfa-interferon in patients with asymptomatic HIV infection and CD4 between 200 and 500.: Int Conf AIDS. 1994 Aug 7-12;10(1):210 (abstract no. PB0270). Unique Identifier : AIDSLINE ICA10/94369726; Tavio M; Colomba A; Izzo E; Pintus A; Puppoa F; Serraino D; Traina C; Tirelli U; Centro di Riferimento Oncologico, Aviano, Italy.; OBJECTIVE: To evaluate the efficacy and the tolerability of AZT plus alfa-Interferon. METHODS: Phase-II study including patients with HIV infection and: CDC group 2 or 3, CD4 count between 200 and 500, Karnofsky index > or = 60. The therapeutic regime
Safety of zalcitabine (ddC) in zidovudine (ZDV) intolerant individuals: results of the European Expanded Access Study.: Int Conf AIDS. 1994 Aug 7-12;10(1):210 (abstract no. PB0268). Unique Identifier : AIDSLINE ICA10/94369727; Moyle G; Goll A; Snape S; F. Hoffmann-La Roche Ltd, Basel, Switzerland.; OBJECTIVE: To assess the tolerability of ddC in HIV positive individuals intolerant to or had clinically failed on ZDV. METHODOLOGY: Patients intolerant to or had failed on ZDV were provided with ddC (0.75 mg tid, weight adjusted) via an open label ac
A phase I/II open label combination study of the tolerability and activity of ddC and interferon alpha in patients with early symptomatic HIV-infection (ANRS protocol 016).: Int Conf AIDS. 1994 Aug 7-12;10(1):211 (abstract no. PB0274). Unique Identifier : AIDSLINE ICA10/94369728; Mouton Y; Goujard C; Raffi F; Seigneurin JM; Bilbault P; ANRS, Paris, France.; OBJECTIVES: Tolerability and antiviral activity of dideoxycytidine alone or in combination with two doses of interferon alpha based on laboratory markers (CD4, Ag P24, 82 microglobulin). To investigate the usefulness of quantitative plasma and cellular viraemia and quantitative RNA and DNA PCR. METHODS: In this open l
Antiviral combined and thymostimulin in patients with HIV stages III and IV. Effects on the CD4 cells counts.: Int Conf AIDS. 1994 Aug 7-12;10(1):211 (abstract no. PB0273). Unique Identifier : AIDSLINE ICA10/94369729; Conde-Mercado JM; Feregrino Goyos M; Eid Lidt G; Torras GV; Gallegos Perez H; Alvarado Diez D; Clinica isi de Norteamerica, Mexico D.F. Mexico.; OBJECTIVES: We tried to evaluate the immunomodulating effects of the TP1 ((Thymostimulin derived from calf thymus) in HIV patients with less than 300 CD4 cells, associating the combination of AZT + DDC or AZT + DDC + NAC. METHODS: We studied 15 patien
First evaluation of 20 HIV positive patients on treatment of AZT/ddI at the L.J. Engelmajer Association.: Int Conf AIDS. 1994 Aug 7-12;10(1):211 (abstract no. PB0275). Unique Identifier : AIDSLINE ICA10/94369730; Puchot P; Puppo P; Engelmajer ML; European Institute for Documentation and Research on Drug Abuse,; Grenade, France.; BACKGROUND: Numerous interesting works published in Berlin demonstrating the benefit of treatments with Combined convergents associating the Nucleoside Analogues ( AZT + DDI or AZT + DDC) taking into consideration the appearance of viral resistance ind
Standard vs low dose ZDV+DDI therapy in PTS with failure of ZDV monotherapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):211 (abstract no. PB0272). Unique Identifier : AIDSLINE ICA10/94369731; Narciso P; Tozzi V; Sette P; Alba L; Grisetti S; Colaiacomo M; Campitelli G; Missori R; Visco G; L. Spallanzani Hosp., USL RM/10 Rome, Italy.; OBJECTIVE. To determine efficacy and tolerance of standard dose (SD) vs low dose (LD) ZDV+DDI combination therapy in patients who failed prior ZDV monotherapy. METHODS. Design: open, prospective, randomized. Eligibility criteria: ZDV monotherapy for more than 6 months,
Impact of AZT monotherapy versus sequential or combination antiretroviral therapy on survival.: Int Conf AIDS. 1994 Aug 7-12;10(1):212 (abstract no. PB0279). Unique Identifier : AIDSLINE ICA10/94369732; Thompson M; Creagh T; Rimland D; Thompson S; Toomey K; AIDS Research Consortium of Atlanta, Inc., Georgia.; OBJECTIVE: To examine the impact of AZT mono- vs sequential or combination antiretroviral therapy on survival. METHODS: Data from the Georgia cohort of the CDC-sponsored Adult Spectrum of Disease Study were analyz
Phase II study of combination of lentinan with ddI in HIV-positive patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):212 (abstract no. PB0276). Unique Identifier : AIDSLINE ICA10/94369733; Kaneko Y; Mimura T; Guralnik M; Baker M; Goodgame J; DeMarzo C; Pierce D; Lang W; Gordon M; Ajinomoto Co., Inc., Tokyo, Japan.; OBJECTIVE: To assess the safety and efficacy of a combination of ddI (didanosine) and lentinan (an immune modulator) in HIV-positive cases. METHODS: Didanosine was administered to HIV-positive individuals with CD4 levels of 200-500 cells/cmm at a dose of 400 mg/day (po) for six weeks (bid), then 2 mg of lentinan iv wa
Synergistic effect of recombinant CD4-immunoglobulin in combination with azidothymidine, dideoxyinosine and 0.5 beta-monoclonal antibody on HIV infection in vitro.: Int Conf AIDS. 1994 Aug 7-12;10(1):212 (abstract no. PB0277). Unique Identifier : AIDSLINE ICA10/94369734; Ushijima H; Ando S; Kitamura T; Muller WE; Dept. of Microbiology, Institute of Public Health, Tokyo, Japan.; OBJECTIVE: To demonstrate the combination therapy of recombinant CD4 (rCD4) or CD4-immunoglobulin (rCD4-IgG) with other compounds is more effective than monotherapy in vitro. MATERIALS AND METHODS: rCD4-IgG (or rCD4),
Zalcitabine (ddC) in combination with zidovudine (ZDV,AZT) for treatment of advanced HIV disease. A multicenter, open label, non comparative study.: Int Conf AIDS. 1994 Aug 7-12;10(1):212 (abstract no. PB0278). Unique Identifier : AIDSLINE ICA10/94369735; Perez-Ancona F; Cano C; Lisker A; Montenegro A; Depto. 65-1 Col. Pedregal de Carrasco, Coyoacan Mexico D.F.; OBJECTIVE: To evaluate the safety, tolerance and efficacy of zalcitabine (2.25 mg/d) plus ZDV (600 mg/d) in patients with advanced HIV-infection with CD4 counts of less than 300 cells/mm3. METHODS: Preliminary analysis includes 84 patients with AIDS or advanced HIV-infection (less than 300 CD4/mm3) who had received th
Human rights in the structure of a social politic in Argentina.: Int Conf AIDS. 1994 Aug 7-12;10(1):213 (abstract no. PB0281). Unique Identifier : AIDSLINE ICA10/94369736; Zalazar A; Work experience of an NGO in a General Hospital of Buenos Aires city included in an association Program between NGO s. The basic question of our investigation was: which is the relationship between AIDS and Human Rights. In which inflection point between the more elementary human rights and the health structures there
The impact of pentoxifylline on quality of life.: Int Conf AIDS. 1994 Aug 7-12;10(1):213 (abstract no. PB0283). Unique Identifier : AIDSLINE ICA10/94369737; Cohen CJ; Dezube BJ; Day JM; Andradas V; Gagnon S; Rieker PP; CRI/New England, Boston, MA.; OBJECTIVE: Pentoxifylline (PTX) has been shown to decrease tumor necrosis factor (TNF) levels in AIDS patients. This study was designed to test the effect of PTX on measures of quality of life (QOL) and surrogate markers. METHODS: A randomized double-blind placebo controlled design was used: patients were on PTX (400
Treatment of HIV infected adult patients with high-dose intravenous immunoglobulin. A randomized trial.: Int Conf AIDS. 1994 Aug 7-12;10(1):213 (abstract no. PB0282). Unique Identifier : AIDSLINE ICA10/94369738; Kiehl M; Heese C; Stoll R; Domschke W; Department of Internal Medicine A, University of Munster,; Germany.; HIV infected patient show a profound deficit of cellular and humoral immunity. This immunologic impairment causes a markedly increased incidence of serious recurrent infections with opportunistic and other microorganisms. Mostly, these infections are difficult to treat and require hospitalization. Intravenous immunogl
Zidovudine or zidovudine/acyclovir in patients with asymptomatic HIV infection and CD4+ cell counts greater than 400/microL.: Int Conf AIDS. 1994 Aug 7-12;10(1):213 (abstract no. PB0280). Unique Identifier : AIDSLINE ICA10/94369739; Jahn S; Busch HW; Reichelt D; Christensen S; Zidek W; Department of Medicine, WWU Munster, Germany.; OBJECTIVE: We have reported earlier that a combination therapy with zidovudine and acyclovir reduces the frequency of herpesvirus infections in patients with severe HIV-disease. 1. To determine the frequencies of herpesvirus infections (HSV, VZV, EBV) 2. The progressio
Effect of cryopreservation on HIV+VE lymphocytes stored for adoptive immunotherapy.: Int Conf AIDS. 1994 Aug 7-12;10(1):214 (abstract no. PB0286). Unique Identifier : AIDSLINE ICA10/94369740; Trickett A; Dwyer J; Tesfamariam N; St. George Hospital, Kogarah, NSW, Australia.; OBJECTIVE: To study the the effect of cryopreservation, storage and thawing on lymphocytes obtained from asymptomatic HIV+ve patients. It is planned that the autologous cryopreserved cells will be expanded and reinfused when the patient progresses towards AIDS, with the aim of delaying disease progression. METHODS: Bl
Effect of fluconazole (FLU) on the clinical pharmacokinetics of delavirdine (DLV).: Int Conf AIDS. 1994 Aug 7-12;10(1):214 (abstract no. PB0284). Unique Identifier : AIDSLINE ICA10/94369741; Cox S; Anderson RD; Driver M; Carel B; Upjohn Company, Kalamazoo, MI.; OBJECTIVE: DLV is a non-nucleoside RT inhibitor with clearance mediated by Cytochrome P4503A. The purpose of this study was to evaluate the effects of multiple doses of FLU, a P4503A inhibitor, on the steady-state pharmacokinetics (PK) of DLV in HIV+patients. METHODS: Thirteen HIV-1 patients (12 males, 1 female; CD4 =
Pentoxifylline decreases TNF in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):214 (abstract no. PB0285). Unique Identifier : AIDSLINE ICA10/94369742; Dezube BJ; Lederman M; Spritzler J; Ahlers C; Pardee AB; Crumpacker CS; Beth Israel Hosp, Boston, MA 02115.; OBJECTIVES: To ascertain if pentoxifylline (Trental, PTX), a tumor necrosis factor (TNF) synthesis inhibitor, can decrease TNF and HIV expression in AIDS patients. METHODS: AIDS patients, on AZT , ddI,
Therapeutic immunization with HIV gp160 in HIV-seropositive patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):214 (abstract no. PB0287). Unique Identifier : AIDSLINE ICA10/94369743; Sandstrom E; Bratt G; Eriksson L; Samuelsson M; Persson C; Volvovitz F; Redfield R; Wigzell H; Wahren B; South Hospital, Karolinska Institute, Stockholm, Sweden.; OBJECTIVE: To evaluate the clinical effect of gp160 immunization in HIV-1 infected individuals. The sample size has been calculated to detect a 50% reduction in progression. METHODS: Since February 1993, 756 patients have been enrolled (February 1994) in a two-year trial of postinfection immunization with HIV-1 rgp160
AHCC immunotherapy in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):215 (abstract no. PB0291). Unique Identifier : AIDSLINE ICA10/94369744; Ghoneum M; Priestly J; Drew Univ. of Med. & Sci., Dept. of Otolaryngology, L.A., CA; 90059.; OBJECTIVE: We evaluated the immunologic effects of Active Hemicellulose Compound (AHCC) therapy for 12 months in 20 HIV positive homosexual men. AHCC is a mycelic extract of Basidiomycota which was originated from hybridization of several types of mushrooms. METHODS: Patients were given AHCC 3 g/d daily. The following
Collection of autologous blood and bone marrow for adoptive immunotherapy in HIV+ve patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):215 (abstract no. PB0288). Unique Identifier : AIDSLINE ICA10/94369745; Trickett A; Dwyer J; Tesfamariam N; Lam-Po-Tang R; Kwan YL; St. George Hospital, NSW, Australia.; OBJECTIVE: To determine the safety of harvesting large quantities of blood +/- bone marrow (BM) from asymptomatic HIV+ve patients. Cells were collected and cryopreserved for subsequent reinfusion when the patient progresses towards AIDS, with the aim of delaying disease onset. METHODS: Blood mononuclear cells were col
Haplotype-matched peripheral blood mononuclear cell (PBMC) transfusions in patients with late-stage AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):215 (abstract no. PB0290). Unique Identifier : AIDSLINE ICA10/94369746; Gomatos PJ; Reiter WM; Keller RH; Cimoch PJ; Vorce DE; Giannetti B; Uribe MR; Center for Special Immunology, Ft. Lauderdale, Florida.; OBJECTIVE: To determine the safety and tolerance of monthly transfusions of haplotype-matched, cross-matched PBMC s in patients with late-stage AIDS. METHODS: Patients with late-stage AIDS and history of multiple OI s or visceral KS received monthly transfusions of HIV hyperimmune plasma in which HIV was inactivated,
The effect of red ginseng and zidovudine on HIV patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):215 (abstract no. PB0289). Unique Identifier : AIDSLINE ICA10/94369747; Cho YK; Kim Y; Choi M; Kim Y; Choi B; Shin Y; Dept. of Microbiol., Univ. of Ulsan, Seoul, Korea.; OBJECTIVE: To assess whether Korean Red Ginseng(RG) affect the immune activation markers and CD4 cells in HIV seropositive individuals (HIV+), and has synergic effect with Zidovudine (ZDV). METHODS: HIV+ (n-145) were randomly selected and divided into 4 groups: no treatment (NO), ZDV, RG, and ZDV+RG combination(COM).
Binational experience in the treatment of AIDS with a low molecular weight natural carbohydrate (ECA-10-142), as stimulant of the immunology system.: Int Conf AIDS. 1994 Aug 7-12;10(1):216 (abstract no. PB0294). Unique Identifier : AIDSLINE ICA10/94369748; Cevallos Arellano E; Ardila Ardila H; Oncology Clinic, Metropolitan Hospital, Quito, Ecuador.; OBJECTIVES: to evaluate the stimulant properties of the immunological system of the (ECA-10-142) a low weight natural origin carbohydrate, in 70 IV CDC stage AIDS patients METHODS: 70 patients with AIDS confirmed diagnosis were selected, 30 Colombians and 40 Ecuadorians, for oral treatment with (ECA-10-142). In a peri
Clinical and immunologic monitoring of HIV-infected patients treated with topical dinitrochlorobenzene (DNCB).: Int Conf AIDS. 1994 Aug 7-12;10(1):216 (abstract no. PB0295). Unique Identifier : AIDSLINE ICA10/94369749; Stricker RB; Elswood BF; Goldberg B; Henry J; Winger EE; Epstein WL; HemaCare Corporation, San Francisco, CA.; There is growing evidence that HIV infection is controlled by cell-mediated immunity (CMI) involving the interaction of dendritic cells, Th1 cells, cytotoxic CD8 T-cells and natural killer (NK) cells. Dinitrochlorobenzene (DNCB) is a skin sensitizing agent that enhances CMI via the induction of delayed-type hypersensi
IL-2 administration to HIV+ individuals and AIDS patients. An open-label study performed in Sao Paulo, Brazil.: Int Conf AIDS. 1994 Aug 7-12;10(1):216 (abstract no. PB0292). Unique Identifier : AIDSLINE ICA10/94369750; Timerman A; Hutzler RU; Albert Einstein Hospital, Sao Paulo, Brazil.; IL-2 is considered an immunomodulatory lymphokine that could play a role in stabilizing the progression of HIV infection in the long-term survivors group of patients, and therefore, would have a role if administered to individuals who are characterized as presenting pr
Passive immunotherapy (PIT) in HIV disease: study of patients and donors.: Int Conf AIDS. 1994 Aug 7-12;10(1):216 (abstract no. PB0293). Unique Identifier : AIDSLINE ICA10/94369751; Bainbridge D; Karpas A; Dept Immunology, Royal London Hospital, UK.; OBJECTIVE: To determine the effects of PIT on patients and donors. METHODS: Plasma was collected from healthy HIV+ individuals with a CD4+ T-cell count of > 450 mm3. Patients with AIDS received 500 ml, those with ARC 250 ml and those with pre-AIDS 150 ml at monthly transfusions. Both donors and patients were monitored
Anti-CMV human immunoglobulins in the prophylaxis of HCMV disease in HIV positive patients. Preliminary results.: Int Conf AIDS. 1994 Aug 7-12;10(1):217 (abstract no. PB0296). Unique Identifier : AIDSLINE ICA10/94369752; Traina C; Magaraci G; Bellissima P; Panella L; Di Stefano G; Zola S; Centro AIDS USL 58, Palermo, Italy.; OBJECTIVE: To prevent or delay the spread of the HCMV disease in HIV positive patients, by using human anti-CMV immunoglobulins. METHODS: Between November 1992 and October 1993, in a 2 phases multicentric clinical trial, were enrolled 48 patients (average age 29 years), including HIV positive patients with CD4+
CD4 cell count of HIV1 asymptomatic patients remains stable under cyclosporin A.: Int Conf AIDS. 1994 Aug 7-12;10(1):217 (abstract no. PB0298). Unique Identifier : AIDSLINE ICA10/94369753; Levy R; Tourani JM; Jais JP; Even P; Andrieu JM; Hopital Laennec Universite Paris V, France.; OBJECTIVE: We conducted a pilot study to test whether CSA could slow-down the CD4 cell count decline in asymptomatic HIV-1 infected patients (pts) by decreasing virus replication associated with CD4 cell activation and/or suppressing virus induced immune/auto-immune phenomena). METHODS: From Sept 1985 to Nov 1987 all
Intra-venous gammaglobulin: a weapon against bacterial infections in HIV infected children?: Int Conf AIDS. 1994 Aug 7-12;10(1):217 (abstract no. PB0297). Unique Identifier : AIDSLINE ICA10/94369754; Della Negra M; Castro IO; Queiroz W; Yu CL; Soraggi Neto C; Paste AA; Vazquez CM; Inst. Infectol. Emilio Ribas, Sao Paulo, Brazil.; OBJECTIVE: To evaluate the effects of discontinuity of the administration of intra-venous Gamma globulin (IVGG) and the possibility of the use of Trimethoprim-Sulfamethoxazole (TMP-SMZ) in the prevention of bacterial infections among pediatric AIDS patients. METHODS: In our service, 80 pediatric AIDS patients (P2-CDC)
Recovery of an advanced AIDS patient from serious end stage with treatment.: Int Conf AIDS. 1994 Aug 7-12;10(1):217 (abstract no. PB0299). Unique Identifier : AIDSLINE ICA10/94369755; Andoh Y; Andoh Clinic, Osaka, Japan.; OBJECTIVE: To treat a patient with seriously advanced AIDS by promoting the host defenses. PATIENT: A 27-year-old woman (170 cm height, 50 kg weight; could not walk unaided) was diagnosed as having serious AIDS (Western blotting, gp160(+), gp110/120(+), gp41(+), p68(+), p51(+), p24(+)) with swallow pain, food intolera
CD4 cell count in HIV-infected patients treated with oral IFN alpha--preliminary report.: Int Conf AIDS. 1994 Aug 7-12;10(1):218 (abstract no. PB0303). Unique Identifier : AIDSLINE ICA10/94369756; Smiatacz T; Dzierbicki A; Trocha H; Zielinska W; DPT, Gdansk School of Medicine, Poland.; OBJECTIVE: Evaluation of the influence of oral IFN (interferon) alpha monotherapy of HIV-infected patients on the CD4 and CD8 cell count in peripheral blood. METHODS: We studied 22 HIV-infected patients with initial CD4 cell count less than 500 per cu mm during a period of 90 days. 11 of them received oral IFN alpha,
Effect of trace elements and vitamins on the immune system and AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):218 (abstract no. PB0300). Unique Identifier : AIDSLINE ICA10/94369757; Michael S; OBJECTIVE: To summarise major research on trace elements and vitamins in immunodeficiency prediction and initial enhancement of the immune system. METHODS: The research summarizes the actions, interactions and antagonism of trace elements and vitamins is presented in a single flow chart. RESULTS: The chart is a world
Homoeopathic drugs as immuno modulators: a study in 34 HIV subjects.: Int Conf AIDS. 1994 Aug 7-12;10(1):218 (abstract no. PB0301). Unique Identifier : AIDSLINE ICA10/94369758; Singh V; Rastogi DP; Dey SK; Rao PK; Regional Research Institute (H), Bombay, India.; OBJECTIVE: To improve the immunological status through restoring the normal CD4 level and clinically control the progression of HIV illness. METHODS: Western blot confirmed HIV subjects (34) with immunological assessment by peripheral lymphocyte immuno phenotyping of different age groups of different sex acquired the
The immunoactivetherapy for patients with positive HIV using glycyrrhizin.: Int Conf AIDS. 1994 Aug 7-12;10(1):218 (abstract no. PB0302). Unique Identifier : AIDSLINE ICA10/94369759; Endo Y; Faculty of Allied Medical Science, Akita, Japan.; OBJECTIVE: Six hemophiliacs with positive HIV treated with glycyrrhizin for 11 years. METHODS: The effects were described as the changes of 1) absolute lymphocyte counts; 2) CD4 counts; 3) CD8 counts and 4) CD4/CD8 ratio. Of course, complete blood counts, liver function, determination of immunoglobulin and etc. were e
Lymphoproliferative responses in HIV-1-seropositive volunteers pre and post immunization with immuno rgp160. European Multinational Immuno AIDS Vaccine Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):219 (abstract no. PB0305). Unique Identifier : AIDSLINE ICA10/94369760; Mannhalter JW; Eibl MM; Wahren B; Blazevic V; Goebel FD; OBJECTIVE: To evaluate antigen- and mitogen-induced lymphoproliferative responses in two groups of HIV-1-seropositive volunteers (randomized according to different CD4 strata: CD4 counts > 500 and CD4 counts from 200 to 500) pre and post immunization with the IMMUNO rgp160 AIDS candidate vaccine. METHODS: In a double-
Therapeutic gp160 vaccine (VaxSyn) in adults with CD4 counts < 400/mm3. Massachusetts DPH gp160 Working Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):219 (abstract no. PB0307). Unique Identifier : AIDSLINE ICA10/94369761; DeMaria A; Coady W; Cohen C; Epstein P; Kunches L; Mayer K; Werner B; Mass. Department of Public Health, Jamaica Plain 02130.; To evaluate the safety and immunogenicity of recombinant gp160 vaccine (VaxSyn, MicroGeneSys) in more advanced HIV disease, 140 patients with CD4 counts
Therapeutic Id vaccination in HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):219 (abstract no. PB0306). Unique Identifier : AIDSLINE ICA10/94369762; Sutor GC; Jurkiewicz E; Hunsmann G; Hirn M; Deicher H; Schedel I; Dept. Int. Medicine, Med. School of Hannover, Ger.; OBJECTIVE: The mAb IOT4a (13B8.2) against the CD4/D1 region implicated in HIV-gp120 binding was previously shown to elicit an HIV-neutralising Ab response in rabbits. This clinical phase Ia trial was initiated in order to assess the safety, toxicity and immunogenicity of the Id vaccine in HIV+ volunteers. METHODS: Tent
Use of DNCB as a immune modulator in HIV positive patients--a pilot study.: Int Conf AIDS. 1994 Aug 7-12;10(1):219 (abstract no. PB0304). Unique Identifier : AIDSLINE ICA10/94369763; Traub A; Irmandade da Santa Casa de Misericordia de Porto Alegre, Brazil.; OBJECTIVE: To evaluate the efficacy of topical DNCB application in patients with early HIV disease. METHODS: This study was performed in a public hospital where patients are routinely seen free of charge. Patients of both genders were included in the sample. All forms of transmissions were included as well. Inclusion t
Acyclovir/zidovudine combination therapy and AIDS survival. Multicenter AIDS Cohort Study.: Int Conf AIDS. 1994 Aug 7-12;10(1):22 (abstract no. 060B). Unique Identifier : AIDSLINE ICA10/94369764; Stein DS; Graham NM; Park LP; Hoover DR; Phair JP; Detels R; Ho M; Saah AJ; Johns Hopkins University, Baltimore, MD 21205.; OBJECTIVE: To examine the effect of acyclovir use on disease progression and survival in zidovudine treated HIV + individuals. METHODS: Prospective cohort study of gay and bisexual men seen semiannually. Intent to treat Cox models were fit to determine the relationship between use of acyclovir (modelled as a time depet
Does counselling help to empower women to negotiate for safer sex.: Int Conf AIDS. 1994 Aug 7-12;10(1):22 (abstract no. 063D). Unique Identifier : AIDSLINE ICA10/94369765; Chanda C; Baggaley R; Phiri M; Kelly M; London School of Hygiene and Tropical Medicine.; OBJECTIVES: 1. To assess behaviour change and barriers to behaviour change in women receiving voluntary HIV counselling and testing (c&t). METHOD: A cohort of 152 women attending for HIV c&t were interviewed at the time of pre-test counselling. They were asked why they wanted to have a test, what behavioural st
The WHEEL Project: assessing a community-based model for preventing HIV/AIDS among women.: Int Conf AIDS. 1994 Aug 7-12;10(1):22 (abstract no. 062D). Unique Identifier : AIDSLINE ICA10/94369766; Wild J; Young P; Rosenshine N; Klein H; NOVA Research, Bethesda, MD.; OBJECTIVES: This presentation will offer a description of the assessment and evaluation methods and a statistical portrait of the 2,794 women recruited for the WHEEL (Women Helping to Empower and Enhance Lives) Project and analysis of follow-up data on 1,953. The WHEEL Project focused on the HIV/AIDS-related risk beha
Women, AIDS and social representation.: Int Conf AIDS. 1994 Aug 7-12;10(1):22 (abstract no. 061D). Unique Identifier : AIDSLINE ICA10/94369767; Alonso L; NEPAIDS/University of S. Paulo.; OBJECTIVE: Brazilian women are consistently found to engage in high rates of unsafe sex. Of all women contaminated by HIV, 65% got it from sexual partners. An educational prevention prog. target specifically to the needs of women from 25 to 35 years old was designed, implemented and evaluated. METHOD: The program was
Efficacy of interferon for chronic hepatitis C in HIV+ patients. HIV/IFN/HCV Spanish Study Group.: Int Conf AIDS. 1994 Aug 7-12;10(1):220 (abstract no. PB0310). Unique Identifier : AIDSLINE ICA10/94369768; Soriano V; Garcia-Samaniego J; Bravo R; Castro A; Gonzalez J; Mtnez-Odriozola P; Colmenero J; Carballo E; Del Romero J; Pedreira J; et al; OBJECTIVE: To determine the efficacy and safety of rIFN therapy for CHC in HIV+ individuals. PATIENTS: 88 HIV+ patients fulfilling clinical and histological criteria for CHC and 27 HIV-negative subjects (controls) suffering CHC. METHODS: rIFN alfa-2b (Intron) 5 megaU was given 3 days a week subcutaneously during 3 mon
Equimmune in treatment of HIV infected patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):220 (abstract no. PB0309). Unique Identifier : AIDSLINE ICA10/94369769; Bissbort SH; Davis H; van Brummelen R; Miller S; OBJECTIVE: The main objective was to test Equimmune; a recently formulated immunostimulant, in HIV infected and AIDS patients. METHODS: An open, multi-center, prospective, clinical trial with 103 patients with laboratory confirmed HIV positivity and a CD4 count at entry lower than 650/ml was done. The patients were ev
Immunotherapy with autologous HIV-specific CTL.: Int Conf AIDS. 1994 Aug 7-12;10(1):220 (abstract no. PB0311). Unique Identifier : AIDSLINE ICA10/94369770; Lieberman J; Fabry JA; Skolnik PR; Parkerson GR; Fong DM; Kagan J; Standiford H; Lee E; Landry B; New England Medical Center, Boston, MA 02111.; OBJECTIVE: To test the feasibility, safety and tolerance of treating patients with CD4 counts of 100-400/mm3 by infusion of HIV-specific CTL in an attempt to bolster host immunity to control the virus. METHODS: The CTL response to HIV-1 is dominated by the recognition of a small number of peptides encoded by HIV-1 str
Inhibition of the antibody response to an HIV vaccine by maternal antibody.: Int Conf AIDS. 1994 Aug 7-12;10(1):220 (abstract no. PB0308). Unique Identifier : AIDSLINE ICA10/94369771; Jelonek M; Maskrey J; Steimer K; Potts B; Higgins K; Keller M; Harbor-UCLA Medical Center, Torrance.; We developed the murine model to assess the effects of passively transferred polyclonal maternal anti-rgp120 antibodies on the subsequent immunization of the offspring with rgp120SF2-CFA. Adult female BALB/c mice were immunized with native, glycosylated rgp120SF2 in complete Freund s adjuvant six weeks prior to mating
Blocking effects of FK506 for apoptotic cell death induced by HIV-gp120.: Int Conf AIDS. 1994 Aug 7-12;10(1):221 (abstract no. PB0315). Unique Identifier : AIDSLINE ICA10/94369772; Sekigawa I; OBJECTIVE: We investigated about blocking effects of FK506 for apoptotic cell death induced by HIV-gp120. METHODS: Normal human PBL were cultured with gp120, at 12 hours intervals, anti-TCR antibodies were added to the culture. Cell viabilities were measured by trypan blue uptakes. To investigate blocking effects of F
Effects of HIV-1 immunogen on clinical status.: Int Conf AIDS. 1994 Aug 7-12;10(1):221 (abstract no. PB0314). Unique Identifier : AIDSLINE ICA10/94369773; Bergman G; Moss R; Petillo J; Slade H; METHODS: HIV-1 Immunogen is a gp 120 depleted, whole inactivated virus administered in Incomplete Freud s Adjuvant (IFA). In a double-blind, randomized, IFA-controlled one year study at nine sites, 103 HIV-1 infected adults received an injections of HIV-1 Immunogen or IFA control at 0, 3, 6 months. Patients had CD4 ce
Treatment with thalidomide in AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):221 (abstract no. PB0312). Unique Identifier : AIDSLINE ICA10/94369774; Klausner JD; Makonkawkeyoon S; Akarasewi P; Kasinrerk W; Nakata K; Kaplan G; NYU Med Cntr. Dept of Med., NY.; OBJECTIVES: To demonstrate that thalidomide reduces the toxic manifestations of tumor necrosis factor (TNF) and decreases HIV-1 viral burden in patients with AIDS. METHODS: In a randomized, double-blind, prospective study, 41 male patients with AIDS and 6 months of weight loss were given 300 mg of thalidomide or place
Zidovudine-thymostimulin association in the treatment of CDC2 and CDC3 HIV-positive patients: the second stage of TP1-AZT Multicenter Study (TAMS-2).: Int Conf AIDS. 1994 Aug 7-12;10(1):221 (abstract no. PB0313). Unique Identifier : AIDSLINE ICA10/94369775; Barbaro G; Barbarini G; De Rosa F; Institute of Infectious Diseases, University La Sapienza, Rome,; Italy.; OBJECTIVE. Aim of TAMS-2 has been to define the therapeutic efficacy and the clinical and immunological effects of the association of low dose zidovudine ( AZT ) and Thymostimulin (TP1) on HIV-positive patients belonging to CDC groups 2 and 3. METHODS.
Clinical studies of xenogeneic HIV-1 immunoglobulin.: Int Conf AIDS. 1994 Aug 7-12;10(1):222 (abstract no. PB0318). Unique Identifier : AIDSLINE ICA10/94369776; Osther KB; Fralick R; Hung CH; Thorn RM; Verigen Inc., Hopkinton, MA.; An intravenously formulated immunoglobulin G (IgG) derived from HIV-1 lysate immunized pigs has been used to treat 16 HIV-1 infected patients with AIDS signs ranging from asymptomatic to severe. All patients with AIDS signs have shown improvement for 2 to 3 months in at least one clinical sign. Placebo controlled, bli
Evaluation of long term oral administration of glycyrrhizin in asymptomatic HIV-1 carrier.: Int Conf AIDS. 1994 Aug 7-12;10(1):222 (abstract no. PB0317). Unique Identifier : AIDSLINE ICA10/94369777; Kinoshita S; Tsujino G; Yoshioka K; Akatani K; Imai M; Ikegami N; Osaka National Hospital, Japan.; OBJECTIVE: The study was aimed at evaluating clinically and immunologically the effectiveness of long-term oral administration of glycyrrhizin (GL) to asymptomatic HIV-1 carrier (AC) to delay or prevent the disease progression. METHODS: Daily dose of 150-225 mg of GL was given to AC hemophiliac patients since 1987 in
Response to N-acetyl-L-cysteine on the CD4-CD8 system.: Int Conf AIDS. 1994 Aug 7-12;10(1):222 (abstract no. PB0319). Unique Identifier : AIDSLINE ICA10/94369778; Focaccia R; Cattapan A; Conceicao O; Buainain R; Salaroli A; Focaccia MT; Fac. Medicina Jundiai, Brazil.; We report the first results of a clinical trial conducted to test the response of CD4-CD8 lymphocyte system to the administration of N-Acetyl-L-Cysteine (NAC). HIV-positive patients bearing CD4 counts
The role of hrGM-CSF in neutropenia due to the cytostatic or ganciclovir treatment.: Int Conf AIDS. 1994 Aug 7-12;10(1):222 (abstract no. PB0316). Unique Identifier : AIDSLINE ICA10/94369779; Szlavik J; Banhegyl D; Miskovits E; Gerlei Z; Saint Laszlo Hospital, Department of Immunology, Budapest,; Hungary.; OBJECTIVE: Identification of the role of human recombinant granulocyte-monocyte stimulating factor in neutropenia due to cytostatic treatment of Kaposi s sarcoma and non-Hodgkin s lymphoma associated with AIDS and that of the effectivity of hrGM-CSF in neutropenia due to ganciclovi
DHEA: a potential treatment for HIV disease.: Int Conf AIDS. 1994 Aug 7-12;10(1):223 (abstract no. PB0322). Unique Identifier : AIDSLINE ICA10/94369780; Hasheeve D; Salvato P; Thompson C; Houston Immuno. Institute, TX.; OBJECTIVE: To evaluate the use of Dehydroepiandrosterone (DHEA), a testosterone precursor with possible immunomodulating affects, as an adjunct therapy for HIV disease. METHODS: 12 pts. with AIDS were treated with DHEA in addition to standard antiviral and prophylactic OI therapy for HIV infection. (2:CD4
Efficacy of AM3 (glifofosfopeptical) as modulator of immunity in the treatment of AIDS.: Int Conf AIDS. 1994 Aug 7-12;10(1):223 (abstract no. PB0320). Unique Identifier : AIDSLINE ICA10/94369781; Ruiz Illescas R; Torrecillas L; Teran L; Centro Medico 20 de Noviembre, Issste: Mexico, City.; OBJECTIVE: Investigate if AM3 has a cytoprotective effect by restoring cytopenias induced by the use of AZT and other chemotherapy drugs, and if AM3 improves response to treatment in AIDS associated neoplasm by stimulating immune system. METHODS: 26 m
Pharmacokinetics of GM-CSF and early leucocyte response after subcutaneous (SC) GM-CSF administration to AIDS patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):223 (abstract no. PB0321). Unique Identifier : AIDSLINE ICA10/94369782; Henrivaux P; Fairon Y; C. H. St Joseph-Esperance, Liege, Belgium.; INTRODUCTION. Our study aimed to assess the pharmacokinetic profile of sc GM-CSF (Leucomax, Schering-Plough ) in AIDS patients, to verify the efficacy of low doses of GM-CSF in AIDS patients and to verify the rapid leucocyte response that we suspected from our clinical experienc
Prevention of severe neutropenia in AIDS patients with intermittent, low-dose G-CSF (filgrastim).: Int Conf AIDS. 1994 Aug 7-12;10(1):223 (abstract no. PB0323). Unique Identifier : AIDSLINE ICA10/94369783; Balbiano R; Degioanni M; Valle M; Crivelli P; Bordino C; Mastinu A; Biglino A; Department of Infectious Diseases, General Hospital, Asti, Italy.; OBJECTIVE: to evaluate the efficacy of an intermittent, low dosage of G-CSF in maintaining neutrophil count above 1000/microliters in AIDS patients at risk of developing severe neutropenia ( 500
Contribution of intravenous immunoglobulins (IVIG) therapy in HIV infected adults.: Int Conf AIDS. 1994 Aug 7-12;10(1):224 (abstract no. PB0325). Unique Identifier : AIDSLINE ICA10/94369784; Mars ME; Quinson AM; North MO; Gallais H; Infectious Diseases Unit, Hopital La Conception, Marseille,; France.; OBJECTIVE: To determine the role of IVIG therapy in HIV infected adults. METHODS: Between november 1992 and july 1993, 35 patients (12 homosexual men, 12 intravenous drug users, 9 heterosexual contaminated, 2 transfusion contaminated; 13 women, 22 men; mean age 36.5 years) were enrolled (group A) in a therapeutic prot
Effect of long term therapy with glycyrrhizin for HIV infection in a hemophilia patient.: Int Conf AIDS. 1994 Aug 7-12;10(1):224 (abstract no. PB0326). Unique Identifier : AIDSLINE ICA10/94369785; Imaizumi M; Goto Y; Tada K; Mori K; Dept. of Pediatrics, Tohoku Univ. School of Medicine, Sendai,; Japan.; Glycyrrhizin (GL), an aqueous extract of licorice root in Chinese medicine, has as an antiinflammatory as well as anti-viral effects. Recently, based on in vitro studies proving anti-HIV effects of GL, a clinical trial of GL for HIV infection has been under investigation. In this report, we present a patient who has b
Human recombinant erythropoietin (r-Hu EPO) for treatment of anemia in HIV-infected children.: Int Conf AIDS. 1994 Aug 7-12;10(1):224 (abstract no. PB0324). Unique Identifier : AIDSLINE ICA10/94369786; Caselli D; Maccabruni A; Fundaro C; Portelli E; De Santis U; Gabiano C; Forni GL; Dept of Pediatrics, IRCCS Policl. San Matteo, Pavia, Italy.; BACKGROUND: Anemia is a frequent complication of HIV infection in children, due both to HIV itself and, even more frequently, to antiretroviral therapy. Treatment with r-Hu EPO was reported in HIV infected adults, but data are not available for pediatric patients. PATIENTS: 10 children (5 males, age 3 months-10 years,
Thalidomide for treatment of aphthous ulcers in patients with HIV.: Int Conf AIDS. 1994 Aug 7-12;10(1):224 (abstract no. PB0327). Unique Identifier : AIDSLINE ICA10/94369787; Ball SC; Sepkowitz KA; Jacobs JL; Center for Special Studies, New York Hospital-Cornell Medical; Center, New York.; OBJECTIVE: To examine the efficacy of thalidomide(Tmd) for treatment of aphthous ulcers(AU) in patients with HIV. METHODS: Case report and review of the English literature by Medline search. RESULTS: We recently successfully treated a 31 year old woman with CD4 10/mm and AU refractory to steroid therapy with 400 mg/d
Beneficial effects of intravenous immunoglobulins (IVIg) in the treatment of ARC patients.: Int Conf AIDS. 1994 Aug 7-12;10(1):225 (abstract no. PB0330). Unique Identifier : AIDSLINE ICA10/94369788; Saint-Marc T; Berra N; Perraud P; Livrozet JM; Fournier F; Touraine JL; Transplantation & Clinical Immunology Unit, Hop. E. Herriot,; Lyon, France.; OBJECTIVE: Evaluation of the clinical and immunological effects of systematic and regular infusions of immunoglobulins in adult patients with a severe deficiency of cell-mediated immunity and of antibody production related to HIV infection. METHODS: Starting in January 1991, 44 ARC patients were enrolled in a prospect
CD4 counts during treatment of herpes simplex infection in HIV-1 infected children.: Int Conf AIDS. 1994 Aug 7-12;10(1):225 (abstract no. PB0328). Unique Identifier : AIDSLINE ICA10/94369789; Fomin J; Voronin E; Rakhmanova A; Popova I; Medical Academy Postgraduate Education, St. Petersburg, Russia.; OBJECTIVE: Herpes simplex virus 1 (HSV 1) infection of skin is the most often clinical symptom in russian children infected HIV-1 by parenteral route. The aim was to examine changes in CD4 counts in the course of HSV 1 infection in children long-term treated by Retrovir (Wellcome). We also wished to compare CD4 counts