The envelope proteins of the human immunodeficiency virus Type-1 expressed on the surface of the env gene-transfected cells of a human CD4-positive leukemic line, with or without vpu, serve as targets for immune attack (Meeting abstract).

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International Association for Comparative Research on Leukemia and Related Diseases, 16th Symposium. July 11-16, 1993, Montreal, Quebec, Canada, A59, 1993.. Unique Identifier : AIDSLINE ICDB/94698656
Ahmad A; Menezes J; Lab. of Immunovirology, Ste-Justine Hosp. and Univ. of Montreal,; 3175 Ste-Catherine Rd., Montreal, Quebec, Canada H3T 1C5

Abstract: A CD4-positive human leukemic cell line (CEM) was transfected with the env gene of the human immunodeficiency virus type-1 (HIV-1). Two expression vectors with or without the potential to encode the regulatory protein vpu were used. The transfected cell clones expressing the env gene-encoded proteins were established. The surface protein gp120 was expressed only in clones transfected with the vpu-positive expression vector, whereas in the clones transfected with vpu-negative vector, gp160 could be demonstrated on the surface of the cells. The latter clones always expressed higher amounts of the transfected gene products. No decrease in the density of the surface CD4 was observed in the two kinds of clones. The envelope proteins expressed on the surface of both kinds of these clones served as target for immune attack which was evidenced by the fact that a significant (p less than 0.05) lysis of the env-gene transfected cells was observed in antibody-dependent cellular cytotoxicity (ADCC) assays using serum from HIV-infected individuals. These clones will be very useful in studying the immunobiology of the envelope proteins of HIV-1 and the interaction between them and vpu in the context of CD4-positive human lymphoid cells. They would also serve as useful tools in studying the humoral as well as cellular immune responses to the envelope proteins of the HIV-1 in HIV-infected individuals.

Keywords: Antibody-Dependent Cell Cytotoxicity Antigens, CD4/*ANALYSIS Gene Expression Gene Products, env/GENETICS/IMMUNOLOGY Gene Products, vpu/GENETICS/*IMMUNOLOGY *Genes, env Human HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY HIV Infections/IMMUNOLOGY HIV-1/GENETICS/*IMMUNOLOGY Protein Precursors/GENETICS/IMMUNOLOGY Tumor Cells, Cultured ABSTRACT
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M9480794

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