SAV, an archaebacterial gene with extensive homology to a family of highly conserved eukaryotic ATPases.

Important note: Information in this article was accurate in 1994. The state of the art may have changed since the publication date.

SAV, an archaebacterial gene with extensive homology to a family of highly conserved eukaryotic ATPases.

J Mol Biol. 1994 Jan 7;235(1):396-401. Unique Identifier : AIDSLINE GENBANK/L17042
Confalonieri F; Marsault J; Duguet M; Laboratoire d'Enzymologie des acides Nucleiques, Universite; Paris-Sud, Orsay, France.

Abstract: Nucleotide sequencing of a region of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius allowed us to identify an open reading frame of 780 amino acids strikingly similar to a family of eukaryotic ATPases, involved in a variety of biological functions. Sequence analysis of the predicted polypeptide revealed 63 to 66% similarity with S. cerevisiae CDC 48p and its related genes in amphibians (p97ATPase) and mammals (Valosin Containing Protein, VCP), all possibly involved in the regulation of the cell cycle. The finding of an archaebacterial equivalent of these proteins with a high degree of similarity suggests that it represents the same gene in these various species. The new archaebacterial ORF, called SAV (S. acidocaldarius VCP-like) exhibited the usual signature of all members of the family, a highly conserved domain of about 200 amino acids, which is duplicated. Thus, apart from the VCP-like proteins, SAV also appeared similar, although less clearly, to other ATPases, members of the family, involved in vesicle-mediated transport (NSF, Sec18p), peroxysome assembly (PAS1p), and gene expression in yeast (SUG1p) and in human immunodeficiency virus (TBP-1). Finally, the discovery of the archaebacterial gene could enlighten not only the evolutionary relationships between the members of this complex ATPase family, but also the cellular function of these proteins, that is presently obscure.

Keywords: Adenosinetriphosphatase/*GENETICS Amino Acid Sequence Animal Base Sequence Comparative Study *Conserved Sequence DNA-Binding Proteins/GENETICS *Evolution *Genes, Bacterial *Genes, Reiterated HIV/GENETICS Mice Molecular Sequence Data Open Reading Frames Phylogeny Restriction Mapping Saccharomyces cerevisiae/GENETICS Sequence Homology, Amino Acid Sulfolobus acidocaldarius/*ENZYMOLOGY/*GENETICS Support, Non-U.S. Gov't Swine Translation, Genetic JOURNAL ARTICLE
940430
M9440235

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1994. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1994. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .