Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
Pathogenesis of avian nephroblastoma induced by MAV-2(O).
Diss Abstr Int [B]; 52(9):4582 1992. Unique Identifier : AIDSLINE ICDB/93682290 Aidarous HM; Colorado State Univ.
Abstract:
Inoculation of B14 strain embryos at 10 days of incubation with the subgroup B myeloblastosis associated virus, MAV-2(O), results in a massive nephroblastoma by 12 wk of age. The purpose of this dissertation was to examine the possible factors that modulate tumor cell phenotype in avian nephroblastomas. The origin of nephroblastoma was tested by morphometric analysis of nephrogenic rests. In MAV-2(O) infected chickens, nephrogenic rests persisted until 7 wk of age, and a transitional state appeared to exist between decline of the nephrogenic rests and the development of nephroblastoma. To test for nephroblastoma inducing capability of MAV-2(O) gene regions, 10-day chick embryos were infected with recombinant viruses constructed by Rose Aurigemma between MAV-2(O) and UR2AV avian leukosis virus. These viruses have different envelope determinants and pathogenic spectra. Morphometric analysis of nephroblastoma areas indicated that the env and LTR portion of MAV-2(O) were sufficient to induce nephroblastoma similar to the parent virus. In situ hybridization demonstrated that the env region of the MAV-2(O) genome was expressed at high levels in tumor tissue. Immunocytochemical staining using the avidin-biotin technique was used to localize the virus and proto-oncogenes c-fos and c-jun in groups of chickens of different ages. The results indicated that p27 expression correlated with the stage of disease. Proto-oncogenes c-fos and c-jun appeared to be involved in the induction of the nephroblastomas. Expression of P105 (RB-1) was examined by the western blot technique using mouse monoclonal antibody C36. The results showed that P105 was expressed in nephroblastomas, ascitic fluid exudate, and at lower levels of expression in lysates from liver and spleen from chickens bearing tumors. The final experiment was a Southern blot analysis for similarities between avian and human Wilms' tumor. The DNA from infected and uninfected chicken kidneys were tested using CAT-10 and PcT-29 human probes at 11P13 of chromosome 11. The results showed a diminished hybridization signal in tumor DNA when compared with control DNA. The conclusions from these experiments are that nephroblastomas originate from nephrogenic rests. Furthermore there appear to be more than one factor modulating tumor phenotype. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD92-07314).
Keywords: Animal Chickens DNA Probes DNA, Neoplasm/METABOLISM Gene Products, env/GENETICS Genome, Viral Kidney Neoplasms/*ETIOLOGY/GENETICS/METABOLISM *Myeloblastosis Virus, Avian/GENETICS Nephroblastoma/*ETIOLOGY/GENETICS/METABOLISM Proto-Oncogene Proteins c-fos/GENETICS/METABOLISM Proto-Oncogene Proteins c-jun/GENETICS/METABOLISM Proto-Oncogene Proteins c-myc/GENETICS/METABOLISM Recombination, Genetic Repetitive Sequences, Nucleic Acid/GENETICS Signal Transduction THESIS 930930
M9391191
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
