The MCR (mycoplasmal cell reactivity), a new concept in cellular infectivity: application as amplification factor for in vitro diagnosis purpose. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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The MCR (mycoplasmal cell reactivity), a new concept in cellular infectivity: application as amplification factor for in vitro diagnosis purpose.

Abstr Gen Meet Am Soc Microbiol. 1993;93:165 (abstract no. G-19). Unique Identifier : AIDSLINE ASM93/93291793
Pautrat G; Regis R; Julia F; Dombrecht S; Escarguel C; Papierok G; International Mycoplasma (Groupe STAGO), ZAC de Signes, France.

Abstract: Mycoplasma has been implied as cofactor in AIDS (MONTAGNIER and lo Groups). We tested different species of Mycoplasma (M. hominis, U. urealyticum, M. orale, M. salivarium, M. fermentans, M. pirium, M. genitalium, etc) on in vitro cellular permissive systems for infectious agents of urogenital and pulmonary tracts (respectively: Chlamydiae Trachomatis/Mac Coy Cells and HSV/Vero Cells, Chlamydiae Twar/Hela Cells and RSV/Hep-2 or Vero Cells). In all cases, according to the kind of mycoplasma used, we got an impressive amplification of the response in terms of cytopathogenicity, antigen labelling (EIA, IF) and infectious titration, compared to unmycoplasmisated Cells (control). Cell mycoplasmisation or absence of mycoplasma cellular contamination was controlled by Dapi fluorescence technique. These results display that Mycoplasm has potentiality for being also in vivo cofactor in other pathologies than AIDS, and allow to use by MCR technology, much more sensitive in vitro cellular models taking into account the in vivo ecosystem, without any amplification of false positive signal.
Keywords: Acquired Immunodeficiency Syndrome/*COMPLICATIONS Animal Carcinoma, Hepatocellular Cell Line Chlamydia/PHYSIOLOGY Chlamydia trachomatis/PHYSIOLOGY Hela Cells Human Liver Neoplasms Mycoplasma/*GROWTH & DEVELOPMENT/ISOLATION & PURIF Mycoplasma Infections/*COMPLICATIONS Sarcoma Viruses, Avian/PHYSIOLOGY Simplexvirus/PHYSIOLOGY Tumor Cells, Cultured Vero Cells ABSTRACTKWDacquiredimmunodeficiencysyndrome/KWDcomplicationsanimalcarcinoma,hepatocellularcelllinechlamydia/physiologychlamydiatrachomatis/physiologyhelacellshumanliverneoplasmsmycoplasma/KWDgrowth&development/isolation&purifmycoplasmainfections/KWDcomplicationssarcomaviruses,avian/physiologysimplexvirus/physiologytumorcells,culturedverocellsabstract
930930
M9391178

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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