Detection of 28 and 65 kDa proteins in Mycobacterium avium-intracellulare complex-infected human macrophages.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Detection of 28 and 65 kDa proteins in Mycobacterium avium-intracellulare complex-infected human macrophages.

Abstr Gen Meet Am Soc Microbiol. 1993;93:180 (abstract no. U-66). Unique Identifier : AIDSLINE ASM93/93291797
Ippoliti F; Fattorini L; Damiani G; Santoro C; Arizzi M; Xiao Y; Orefici G; La Sapienza University, Rome, Italy.

Abstract: Organisms belonging to Mycobacterium avium-intracellulare complex (MAC) are the most common bacterial pathogens isolated from acquired immunodeficiency syndrome (AIDS)-patients. MAC are usually present within tissue macrophages where they proliferate with little evidence of inflammatory response. To investigate if members of the heat shock proteins released by mycobacteria during growth are available for immune recognition we monitored the presence of both 28 and 65 kDa proteins in a model of CD68+ human macrophages infected with MAC transparent colonial variant (serovar 21 from an AIDS patient) and cultured for 10 days. To this end H60 and H105 monoclonal antibodies recognizing M. tuberculosis 28 and 65 kDa proteins, cross-reactive with MAC in ELISA test, were used in an immunocytochemical assay. MAC efficiently proliferated within cells and the ratio MAC/macrophage increased from approximately 1 to 10 during incubation. Macrophage positivity for 65 kDa and 28 kDa proteins was 3, 48, 64, 50% and 0, 15, 21, 48%, respectively on days 0, 5, 7, 10, suggesting a modulated expression of the two antigens during infection. Uninfected or LPS-treated macrophages were negative. Immunocytochemistry do not allow to discriminate between surface or cytoplasmic nature of the two proteins detected however the discrepancy between bacterial replication and expression of the 65 kDa protein at 10 days seems to indicate a surface more than cytoplasmic localization of this antigen.

Keywords: Antibodies, Bacterial Antibodies, Monoclonal Antigens, CD/IMMUNOLOGY Antigens, Differentiation, Myelomonocytic/IMMUNOLOGY AIDS-Related Opportunistic Infections/IMMUNOLOGY/*MICROBIOLOGY Cells, Cultured Heat-Shock Proteins/ANALYSIS/*BIOSYNTHESIS Human Macrophages/IMMUNOLOGY/*MICROBIOLOGY Molecular Weight Mycobacterium avium Complex/GROWTH & DEVELOPMENT/ISOLATION & PURIF/*PHYSIOLOGY Mycobacterium avium-intracellulare Infection/ETIOLOGY/ *MICROBIOLOGY Mycobacterium tuberculosis/IMMUNOLOGY Support, Non-U.S. Gov't ABSTRACT
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M9391174

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