Restriction endonuclease patterns of non-serotypable adenoviruses recovered from AIDS patients.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Restriction endonuclease patterns of non-serotypable adenoviruses recovered from AIDS patients.

Abstr Gen Meet Am Soc Microbiol. 1993;93:429 (abstract no. S-13). Unique Identifier : AIDSLINE ASM93/93291807
Chegas PM; Pierce CS; Riepenhoff-Talty MR; Amsterdam D; Children's Hospital of Buffalo, N.Y.

Abstract: Over a two year period, 25 adenovirus (AV) isolates were recovered from fecal samples of 14 HIV-seropositive males. Specimens were inoculated into A-549 cells. Virus replication as evidenced by cytopathic effect was confirmed by specific AV immunofluorescent staining. Using conventional serum neutralization (SN) three isolates were identified using antisera against AV serotypes 1-39; 14 were identified using antisera against the new candidate strains, 42-47; and eight could not be identified. To further characterize the AD isolates, restriction endonuclease digestion of viral DNA was performed. Viral DNA was extracted from infected cells using a modification of the Hirt procedure, purified, concentrated and then subjected to digestion with restriction endonucleases, HIND III, BAM HI and Sma I. Restriction fragments separated by agarose gel electrophoresis were stained with ethidium bromide and viewed under UV-illumination. Analysis of the gel patterns confirmed the SN results and demonstrated that isolates obtained from the same patients had identical genotypes, and that the genotypes of isolates varied among different patients. Four unique restriction patterns were found among the eight non-serotypable viral isolates. These may represent new AV enteric pathogens associated with immunosuppressed patients.

Keywords: Adenoviruses, Human/GENETICS/*ISOLATION & PURIF/*PHYSIOLOGY Cell Line DNA, Viral/*ANALYSIS/GENETICS/ISOLATION & PURIF Electrophoresis, Agar Gel Feces/MICROBIOLOGY Human HIV Seropositivity/*MICROBIOLOGY Immune Sera Male Restriction Mapping Virus Replication ABSTRACT
930930
M9391164

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