Chemiluminescent detection of PCR amplified HIV-1 DNA from peripheral blood mononuclear cells of migrant farm workers.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Chemiluminescent detection of PCR amplified HIV-1 DNA from peripheral blood mononuclear cells of migrant farm workers.

Abstr Gen Meet Am Soc Microbiol. 1993;93:435 (abstract no. T-17). Unique Identifier : AIDSLINE ASM93/93291818
Bankowski MJ; Schulte J; Frees N; Horton P; Landay A; Staes B; Shuburg R; Caueffield D; Knapp R; Glinski B; et al; Diagnostic Services, Inc., Naples Community Hospital, FL.

Abstract: A recent study of migrant farm workers in Florida showed a high seropositivity rate for both HIV-1 (5%) and syphilis (8%) [CDC. MMWR 1992;41:723-725]. We selected a subgroup of 35 from these 310 participants to further investigate with both HIV-1 co-culture and the polymerase chain reaction (PCR). Our patient group contained 9/35 (HIV IFA+/FTA-), 16/35 (HIV IFA-/FTA+), 4/35 (HIV IFA+/FTA+) and 6/35 (HIV IFA-/FTA-). An HIV primer set (SK145/SK431) capable of amplifying both HIV-1 and HIV-2 DNA was used in the PCR. PCR product was detected using southern hybridization with an alkaline phosphatase-labeled SK102 probe. This nonradioactive chemiluminescent detection format was capable of detecting 2 copies of HIV-1 DNA contained in the original PCR reaction mix. Our results show the following: 1. PCR product was not detected and HIV was not cultured in neither the HIV IFA-/FTA+ or HIV IFA-/FTA- groups. 2. PCR, compared to HIV IFA, showed a 92.3% sensitivity and a 100% specificity and HIV co-culture 69.2% and 100% respectively. The single HIV IFA+ specimen that gave a negative HIV PCR reaction also showed a weak reaction in the beta globin PCR control reaction and was co-culture negative. In conclusion, our results indicate that this nonradioactive chemiluminescent detection format is both sensitive and specific.

Keywords: Chemiluminescence Comparative Study DNA, Viral/*BLOOD/GENETICS Florida Human HIV Seropositivity/BLOOD/*DIAGNOSIS HIV-1/GENETICS/*ISOLATION & PURIF Leukocytes, Mononuclear/*MICROBIOLOGY Polymerase Chain Reaction/*METHODS *Transients and Migrants ABSTRACT
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