Evaluation of HIV-1 perinatal infection by a modified HIV-1 IgA immunoblot assay. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Evaluation of HIV-1 perinatal infection by a modified HIV-1 IgA immunoblot assay.

Abstr Gen Meet Am Soc Microbiol. 1993;93:435 (abstract no. T-20). Unique Identifier : AIDSLINE ASM93/93291819
Norman GL; Gross M; Yee J; Carlson J; Microbiology Reference Laboratory, Cypress, CA.

Abstract: Approximately 13-45% of children born to HIV-infected mothers are expected to become infected with HIV-1. Serological diagnosis of infection in infants is confounded by the presence of passively acquired maternal IgG. Recent reports have documented the efficacy of detecting IgA antibodies to HIV-1 for the early diagnosis of HIV in infants. Since IgA does not cross the placenta, the presence of anti-HIV IgA indicates HIV infection of the child. Using our modified assay, we tested 4 seropositive-culture negative and 6 seropositive-culture positive specimens from infants ranging in age from 3 days to 1 year. Each specimen was tested in parallel by both IgG and IgA immunoblot assays. Prior to IgA testing, IgG antibody was removed from specimens by adsorption with recombinant protein G-agarose. No IgA reactivity was detected in any of the 4 culture-negative specimens, despite strong anti-HIV IgG reactivity in the untreated specimens. Three of the culture-positive specimens showed IgA reactivity. The HIV-1 IgA immunoblot assay demonstrated high specificity (100%) and appears a valuable tool for the rapid detection of HIV-1 infection in many infants.
Keywords: Acquired Immunodeficiency Syndrome/BLOOD/*DIAGNOSIS/IMMUNOLOGY Female Human HIV Antibodies/*BLOOD HIV Seropositivity/BLOOD/*DIAGNOSIS/IMMUNOLOGY *HIV-1 IgA/*BLOOD IgG/*BLOOD Immunoblotting/METHODS Infant, Newborn Pregnancy Pregnancy Complications, Infectious/*IMMUNOLOGY ABSTRACTKWDacquiredimmunodeficiencysyndrome/blood/KWDdiagnosis/immunologyfemalehumanhivantibodies/KWDbloodhivseropositivity/blood/KWDdiagnosis/immunologyKWDhiv-1iga/KWDbloodigg/KWDbloodimmunoblotting/methodsinfant,newbornpregnancypregnancycomplications,infectious/KWDimmunologyabstract
930930
M9391152

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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