[Immunoenzymatic labeling of monoclonal antibodies for surface antigens of T-cells using immune complexes of APAAP in patients with interstitial lung disease]

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

[Immunoenzymatic labeling of monoclonal antibodies for surface antigens of T-cells using immune complexes of APAAP in patients with interstitial lung disease]

Chung Hua Nei Ko Tsa Chih. 1992 Dec;31(12):748-51, 779. Unique Identifier : AIDSLINE MED/93321514
Hou XM; Xin H; Zhao HW; Respiratory Disease Research Institute, China Medical University,; Shenyang.

Abstract: The use of unlabeled antibody bridging technique with alkaline phosphatase monoclonal anti-alkaline phosphatase (APAAP) complexes makes it possible to solve the problem of short durability of immunofluorescent staining and the problem of nonspecific endogenous enzyme interference of blood cells with immunoperoxidase method. The technique of APAAP allows satisfactorily to demonstrate the cytoplasmic and surface membrane antigens of T-cells both in peripheral blood and bronchoalveolar lavage fluid (BALF). With the technique studied, the subsets of T-lymphocytes simultaneously in both peripheral blood and BALF of 26 patients with interstitial lung disease and of 16 apparently healthy subjects. The results showed: (1) In patients with interstitial pulmonary fibrosis (IPF) CD8 cells in BALF were higher in number than those in peripheral blood and BALF of normal subjects (P < 0.01). It is suggested that abnormalities of T-Lymphocytes might also play a role in the pathogenesis of IPF. (2) CD4 cells in BALF of patients with sarcoidosis were significantly higher in number than those in other groups (P < 0.01). However, CD8 cells in BALF of patients with sarcoidosis were lower in number than those in others (P < 0.01). The higher ratio of CD4/CD8 was found in sarcoidosis patients during active stage. The findings suggested that change of the ratio of CD4/CD8, as an immunoregulatory abnormalities in lung, could be regarded as one of parameters in assessing the activity in patients with sarcoidosis.

Keywords: Adult Aged Alkaline Phosphatase Antibodies, Monoclonal Antigens, Surface/*ANALYSIS Bronchoalveolar Lavage Fluid/CYTOLOGY *CD4-CD8 Ratio English Abstract Female Human Immunoenzyme Techniques Lung Diseases/IMMUNOLOGY Male Middle Age Pulmonary Fibrosis/*IMMUNOLOGY Sarcoidosis/IMMUNOLOGY T-Lymphocyte Subsets/*IMMUNOLOGY T-Lymphocytes/IMMUNOLOGY JOURNAL ARTICLE
931030
M93A0760

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