Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
Lymphocyte activation in HIV-1 infection. I. Predominant proliferative defects among CD45R0+ cells of the CD4 and CD8 lineages.
AIDS. 1993 May;7(5):613-24. Unique Identifier : AIDSLINE MED/93305206 Janossy G; Borthwick N; Lomnitzer R; Medina E; Squire SB; Phillips AN; Lipman M; Johnson MA; Lee C; Bofill M; Department of Clinical Immunology, Royal Free Hospital and School; of Medicine, London, UK.
Abstract:
OBJECTIVES AND DESIGN: The proliferative defects of CD4 and CD8 cells taken from 474 HIV-1-seropositive individuals during various stages of disease were quantitated. Phytohaemagglutinin (PHA) and soluble anti-CD3 were used in optimal mitogenic concentrations in the presence of recombinant interleukin-2 (rIL-2) and conditioned medium, and the proliferation of cells from HIV-1-seropositive donors was assessed in co-culture with HIV-1-seronegative cells in order to exclude effects of cytokine deficiency. Defects within the CD45RA+ ('unprimed') and CD45R0+ ('primed') T-cell populations were also investigated. METHODS: Quantitative immunofluorescence and double and triple labelling in flow cytometry were performed for (1) CD25 (IL-2 receptor alpha chain) expression, (2) lymphocyte and T-cell survival, and (3) blast transformation and proliferation--in relation to the original input of cells for each subpopulation. RESULTS: T cells from normal and HIV-1-seropositive donors were CD25+ at day 1. In HIV-1-seropositive patients a variable number of CD4 and CD8 lymphocytes failed to further increase CD25, and died as a sign of activation-associated lymphocyte death (AALD). Forty-two per cent of asymptomatic subjects, including 32% of those with CD4 cell counts > 400 x 10(6)/l, showed a poor blast transformation (< 30% blasts). Cells from HIV-1-seropositive donors showed poor blast responses when co-cultured with HIV-1-seronegative cells; both CD4 and CD8 cells were handicapped. In asymptomatic HIV-1-seropositive people T cells with the CD45R0+ RA- ('primed') phenotype were three to five times more vulnerable to AALD than the CD45RA+ RO- ('unprimed') cells. In patients in Centers for Disease Control and Prevention (CDC) disease stage IV both CD45R0+ and -RA+ populations were severely affected. CONCLUSIONS: This is the first quantitative analysis to demonstrate that in HIV-1 infection mitogen-stimulated CD45R0+ ('primed') T cells preferentially die upon activation. Both the CD4 and CD8 lineages are affected, as seen in animal models of graft versus host disease. AALD may explain defects of immunological memory. The analysis of AALD may be a suitable assay for studying whether antiviral drugs influence the proliferative responses of lymphocytes.
Keywords: Antigens, CD3 Antigens, CD4 Antigens, CD45 Antigens, CD8 Cell Death Female Human HIV Infections/*IMMUNOLOGY/PATHOLOGY HIV Seropositivity/IMMUNOLOGY *HIV-1 In Vitro *Lymphocyte Transformation Male Phytohemagglutinins/PHARMACOLOGY Support, Non-U.S. Gov't T-Lymphocyte Subsets/*IMMUNOLOGY/PATHOLOGY JOURNAL ARTICLE 931030
M93A0750
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Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
