Envelope glycoproteins of HIV-1, HIV-2, and SIV purified with Galanthus nivalis agglutinin induce strong immune responses. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Envelope glycoproteins of HIV-1, HIV-2, and SIV purified with Galanthus nivalis agglutinin induce strong immune responses.

AIDS Res Hum Retroviruses. 1993 May;9(5):431-8. Unique Identifier : AIDSLINE MED/93305382
Gilljam G; Department of Virology, National Bacteriological Laboratory,; Stockholm, Sweden.

Abstract: Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.
Keywords: Animal Antibodies, Viral/BIOSYNTHESIS Antigens, CD4 Antigens, Viral/ISOLATION & PURIF Gene Products, env/IMMUNOLOGY/*ISOLATION & PURIF Human HIV Antibodies/BIOSYNTHESIS HIV Antigens/ISOLATION & PURIF HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY Lectins Macaca fascicularis Mice Mice, Inbred BALB C Neutralization Tests SIV/*IMMUNOLOGY JOURNAL ARTICLEKWDanimalantibodies,viral/biosynthesisantigens,cd4antigens,viral/isolation&purifgeneproducts,env/immunology/KWDisolation&purifhumanhivantibodies/biosynthesishivantigens/isolation&purifhiv-1/KWDimmunologyhiv-2/KWDimmunologylectinsmacacafascicularismicemice,inbredbalbcneutralizationtestssiv/KWDimmunologyjournalarticle
931030
M93A0726

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