Molecular cloning and developmental regulation of Dictyostelium discoideum homologues of the human and yeast HIV1 Tat-binding protein. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Molecular cloning and developmental regulation of Dictyostelium discoideum homologues of the human and yeast HIV1 Tat-binding protein.

Biochem Biophys Res Commun. 1993 Jun 30;193(3):1291-6. Unique Identifier : AIDSLINE GENBANK/S64637
Shaw DR; Ennis HL; Roche Institute of Molecular Biology, Roche Research Center,; Nutley, NJ 07110.

Abstract: TBP1 and 7, two human cDNA clones, are members of a large, highly conserved family that encode proteins that bind to type 1 human immunodeficiency virus Tat protein, and may be transcription factors. Two Dictyostelium discoideum homologues, denoted DdTBP2 and 10, were isolated and characterized, and these have striking sequence similarity with both the human and yeast counterparts of the family. RNA blot analysis indicates that both DdTBP2 and 10 are developmentally regulated. Transcripts representing these cDNAs are highest in vegetatively growing cells, and their levels fall steadily throughout multicellular development and are not found in dormant or germinating spores. If indeed these proteins in Dictyostelium are also transcription factors, then this finding might indicate that only certain developmentally regulated genes require them.
Keywords: Amino Acid Sequence Animal Base Sequence Cloning, Molecular/METHODS Comparative Study Dictyostelium/GROWTH & DEVELOPMENT/GENETICS/*METABOLISM DNA-Binding Proteins/*BIOSYNTHESIS/GENETICS DNA, Fungal/GENETICS *Gene Expression Regulation, Fungal Gene Products, tat/*METABOLISM Human HIV-1/*METABOLISM Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction RNA, Messenger/*METABOLISM Saccharomyces cerevisiae/GENETICS/*METABOLISM Sequence Homology, Amino Acid JOURNAL ARTICLEKWDaminoacidsequenceanimalbasesequencecloning,molecular/methodscomparativestudydictyostelium/growth&development/genetics/KWDmetabolismdna-bindingproteins/KWDbiosynthesis/geneticsdna,fungal/geneticsKWDgeneexpressionregulation,fungalgeneproducts,tat/KWDmetabolismhumanhiv-1/KWDmetabolismmolecularsequencedataoligodeoxyribonucleotidespolymerasechainreactionrna,messenger/KWDmetabolismsaccharomycescerevisiae/genetics/KWDmetabolismsequencehomology,aminoacidjournalarticle
931030
M93A0629

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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