Topoisomerase I and II cleavage sites in the long terminal repeat and genomic integration region of HIV-1 (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Topoisomerase I and II cleavage sites in the long terminal repeat and genomic integration region of HIV-1 (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A2521 1993. Unique Identifier : AIDSLINE MED/93693474
Pommier Y; Poddevin B; Jenkins J; Kohn KW; Lab. of Molecular Pharmacology, DTP, DCT, NCI, Bethesda, MD 20892

Abstract: Proviral promoters are located at the U3/R junction of the long terminal repeats (LTR). The present study is the first mapping and sequencing of topoisomerase (topo) cleavage sites in the U3/R LTR region of HIV-1 DNA using a plasmid containing the LTR integrated in a segment of genomic DNA. We find that amsacrine and teniposide induce specific topo II cleavage patterns, but that strong common sites reside preferentially at the promoter site and in the TATAA box. This is consistent with our previous finding of an intense top2 cleavage site in the human c-myc promoter (Cancer Res 52:3125, 1992). The first 100 bases upstream from the promoter and containing the Sp1 and NF-kB sites are poorly cleaved, and cleavage in the integration region does not coincide precisely with the junction of viral and genomic DNA. Topo I cleavage sites induced by camptothecin occur preferentially in the TAR and 100-103 bp upstream from the promoter. These findings demonstrate preferential topo I and II binding in the HIV-1 DNA transcription-regulatory region, and suggest potential interactions between cellular topoisomerases and the multiple factors involved in HIV-1 transcription and replication.
Keywords: *DNA Topoisomerase/GENETICS *DNA Topoisomerase (ATP-Hydrolysing)/GENETICS *HIV-1/GENETICS *Repetitive Sequences, Nucleic Acid *Transcription, GeneticKWDdnatopoisomerase/geneticsKWDdnatopoisomerase(atp-hydrolysing)/geneticsKWDhiv-1/geneticsKWDrepetitivesequences,nucleicacidKWDtranscription,genetic
931130
M93B5840

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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