Characterization of T-cell receptor V beta repertoire in ovarian tumor infiltrating lymphocytes (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Characterization of T-cell receptor V beta repertoire in ovarian tumor infiltrating lymphocytes (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A2736 1993. Unique Identifier : AIDSLINE MED/93693679
Fisk B; Tucker SL; Pollack MS; Flytzanis CN; Ioannides CG; UT M.D. Anderson Cancer Center, Houston, TX 77030

Abstract: Tumor infiltrating lymphocytes (TIL) cultured in media containing IL-2 were shown to mediate antitumor responses. We used polymerase chain reaction to characterize the TCR V beta repertoire of ovarian TIL isolated from three tumor sites of the same patient at the same time cultured under identical conditions, resulting in CD3+ cell lines with similar CD8:CD4 ratios. TIL isolated from ovary and ascites expressed a broad distribution of V beta repertoire, while the V beta phenotype of the TIL from secondary tumor (omentum) was more restricted and dominated by the V beta-1, -11 and -14 families. Importantly, the percentage of the V beta-11 family expression in omentum and ovary TIL at 3 and 5 mo was found to correlate with the levels of lysis of the tumor localized to omentum (p=0.003), followed by the lysis of primary (p=0.031) and ascites (p=0.040) tumors. The level of V beta-1 family expression in the same TIL correlated only with the lysis of the omentum tumor (p=0.014). Furthermore, in a larger group of CD8+ TIL lines, % V beta-3 family expression correlated with lysis of autologous tumor in 4/6 samples (p less than 0.03). This is the first documentation of correlation between V beta usage and tumor lysis, by effectors from different tumors.
Keywords: *Lymphocytes, Tumor-Infiltrating/IMMUNOLOGY *Ovarian Neoplasms/IMMUNOLOGY *Receptors, Antigen, T-Cell/IMMUNOLOGYKWDlymphocytes,tumor-infiltrating/immunologyKWDovarianneoplasms/immunologyKWDreceptors,antigen,t-cell/immunology
931130
M93B5838

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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