In vitro infection of human macrophages by human T-cell leukemia/lymphotropic virus type I (HTLV-I).

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

In vitro infection of human macrophages by human T-cell leukemia/lymphotropic virus type I (HTLV-I).

AIDS Res Hum Retroviruses. 1992 Nov;8(11):1845-9. Unique Identifier : AIDSLINE MED/93143992
Koralnik IJ; Lemp JF Jr; Gallo RC; Franchini G; Laboratory of Tumor Cell Biology, National Cancer Institute,; National Institutes of Health, Bethesda, MD 20892.

Abstract: HTLV-I is associated with a neurological syndrome designated Tropical Spastic Paraparesis/HTLV-I associated myelopathy (TSP/HAM). To determine whether HTLV-I can replicate in human primary macrophages and thus contribute to HTLV-I dissemination in the nervous system, elutriated human macrophages were infected cell-free with the HTLV-ICR and HTLV-IBOU isolates from patients with adult T-cell leukemia and TSP/HAM, respectively. Viral production was monitored by measuring the viral p24 gag antigen in the cell culture supernatant, by electron microscopy (EM) and by polymerase chain reaction (PCR) on viral DNA and RNA. The HTLV-I p24 gag antigen was detected 21 days after infection with either isolate, and the presence of mature viral particles was demonstrated by electron microscopy one month after infection. Viral sequences were amplified by PCR analysis of the infected macrophages' DNA. Spliced mRNAs for the p40tax and p27rex proteins, as well as the p12I, and p30II proteins encoded by the pX region were readily identified by reverse transcriptase PCR. Altogether, these data indicate that HTLV-I replication occurs in vitro in primary human macrophages. Whether macrophage infection occurs also in vivo and is a crucial step in the induction of the neurological manifestations observed in TSP/HAM remains an open question.

Keywords: Base Sequence DNA, Viral/ANALYSIS Human HIV Core Protein p24/ANALYSIS HTLV-I/*GROWTH & DEVELOPMENT Leukemia, T-Cell/MICROBIOLOGY Macrophages/*MICROBIOLOGY/PATHOLOGY Molecular Sequence Data Paraparesis, Tropical Spastic/MICROBIOLOGY Polymerase Chain Reaction RNA Splicing RNA, Messenger/ANALYSIS RNA, Viral/ANALYSIS Support, Non-U.S. Gov't Virus Replication JOURNAL ARTICLE
930530
M9350975

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