Elution mode of Pneumocystis carinii cysts in gravitational field-flow fractionation.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Elution mode of Pneumocystis carinii cysts in gravitational field-flow fractionation.

J Chromatogr. 1992 Aug 7;579(1):143-52. Unique Identifier : AIDSLINE MED/93077740
Bories C; Cardot PJ; Abramowski V; Pous C; Merino-Dugay A; Baron B; Mougeot G; Laboratoire de Chimie Analytique et d'Electrochimie Organique,; Universite Paris-Sud, Chatenay-Malabry, France.

Abstract: The simplest field-flow fractionation technique, i.e. gravitational, was used in an attempt to purify a Pneumocystis carinii cyst suspension. This parasite is an opportunistic invader in immunocompromised patients, especially those suffering from AIDS. The cyst stage is spherical and 5 microns in diameter. Unexpected retention times, not systematically related to the size and the density of the parasite, were obtained under various experimental conditions. When silicone-coated walls were used, Pneumocystis carinii cysts were eluted in the void volume, whereas when uncoated walls were used with a sodium dodecyl sulphate-enriched carrier phase, retention was observed. These phenomena are probably related to the high degree of hydrophobicity of these micrometre-sized biological particles; this degree can be easily determined. The use of the gravitational field-flow fractionation technique can be of a great interest for the development of new methods for diagnostic purposes. Particle-wall interactions and their modifications due to the carrier phase or to the wall treatment can be employed in the search for new bronchoalveolar lavage solutions.

Keywords: Animal Bronchoalveolar Lavage Fluid/MICROBIOLOGY Centrifugation Cysts/*MICROBIOLOGY/PATHOLOGY Female Fractionation/*METHODS *Gravitation Human Lung Diseases/MICROBIOLOGY Pneumocystis carinii/*ISOLATION & PURIF Pneumonia, Pneumocystis carinii/PATHOLOGY Pulmonary Alveoli/MICROBIOLOGY Rats Rats, Wistar JOURNAL ARTICLE $KWDmicrobiology/pathologyfemalefractionation/$
930330
M9331060

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