Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
Pathogenesis of SIVmac251 after atraumatic inoculation of the rectal mucosa in rhesus monkeys.
Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 1. Unique Identifier : AIDSLINE PRIM10/93200939 Pauza CD; Emau P; Salvato MS; Trivedi P; Mackenzie D; Malkovsky M; Schultz KT; Wisconsin Regional Primate Research Center, University of; Wisconsin-Madison 53706.
Abstract:
One year old juvenile male rhesus monkeys were infected with SIVmac251 via atraumatic inoculation of the rectal mucosa. Virus doses ranging from 1,000 to 0.1 tissue culture infectious doses (determined on CEMx174 cells) established infection in all animals. Three of four animals receiving 1,000 or 100 doses became seropositive and were positive for virus isolation from PBMC despite the complete absence of clinical signs of disease for at least 11 months. Six of six animals receiving 10, 1, or 0.1 doses of virus were only rarely (one in five assays) positive for SIV DNA in PBMC by a one-cycle PCR assay for LTR sequences. The low dose animals remained negative for serum antibody (for at least 11 months) and we were unable to culture SIV from unfractionated PBMC. All six animals in the low dose group were remarkable for prolonged periods of weight loss (up to 200 days after infection) and persisting inability to control body weight. Infection in the low dose group of animals was confirmed by direct transfusion of 10 ml whole blood from a seronegative, virus isolation-negative, sporadic PCR-positive animal into a naive recipient and by sequencing of cloned viral DNA sequences. Transfusion established a vigorous infection within 14 days. All viral DNA sequences were highly homologous to the published sequences of SIVmac239 and 251. Animals infected via the rectal mucosa at high dose became viremic and seropositive yet failed to evolve clinical signs of disease within 11 months. Animals exposed to low doses of virus were negative for laboratory markers of infection and failed to show clinical signs of disease other than fluctuations in body weight. The ability to forestall disease progression was common to all animals infected via the rectal mucosa. In addition, animals receiving low dose infections were able to limit virus replication in PBMC despite the complete absence of serum antibody.
Keywords: Animal Antibodies, Viral/BLOOD DNA, Viral/GENETICS/ISOLATION & PURIF Intestinal Mucosa/*MICROBIOLOGY Macaca mulatta Male Polymerase Chain Reaction/METHODS Rectum/*MICROBIOLOGY Simian Acquired Immunodeficiency Syndrome/*PHYSIOPATHOLOGY SIV/GENETICS/ISOLATION & PURIF/*PATHOGENICITY ABSTRACT 930630
M9361081
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Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
