In vivo tracking of lymphocytes in rhesus macaques.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

In vivo tracking of lymphocytes in rhesus macaques.

Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 105. Unique Identifier : AIDSLINE PRIM10/93200944
Salvato M; Johnson E; Rater M; Pauza CD; Dept. of Pathology and Lab. Medicine, Wisconsin Regional Primate; Research Center, Madison 53706.

Abstract: Our goal is to develop lymphocyte replacement therapy in SIV-infected rhesus macaques. Pilot studies to determine the efficacy of storing, culturing and re-introducing lymphocytes monitored the distribution and half-life of re-introduced lymphocyte cultures. Lymphocytes were isolated from rhesus monkeys and marked either with a fluorescent dye or retroviral vectors and tracked in vivo. Dye-labeled cells were re-introduced into the original monkeys, and either monitored by blood draws over a 3 month period, or identified within peripheral organs upon autopsy. Optimum conditions for culturing and labeling lymphocytes were determined. Labeled lymphocytes could be detected in the circulation for at least 100 days by either flow cytometry or fluorescence microscopy if dye labeled or by polymerase chain reaction if labeled with a retroviral vector.

Keywords: Animal Cells, Cultured Flow Cytometry/METHODS Fluorescent Dyes Genetic Vectors Lymphocytes/IMMUNOLOGY/PATHOLOGY/*PHYSIOLOGY Macaca mulatta Microscopy, Fluorescence/METHODS Polymerase Chain Reaction/METHODS Retroviridae/GENETICS Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY/THERAPY ABSTRACT
930630
M9361076

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1993. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1993. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .