Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
Transmission of SIVMne in adult Macaca nemestrina.
Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 106. Unique Identifier : AIDSLINE PRIM10/93200949 Thompson J; Kuller L; Watanabe R; Firpo P; Morton WR; Regional Primate Research Center, University of Washington,; Seattle 98195.
Abstract:
We have previously reported results of a pilot study which indicated the heterosexual transmission of the simian immunodeficiency virus (SIVMne) from female to male pigtailed macaques (M. nemestrina). To better quantify this mode of transmission and to exclude other factors of transmission such as biting and scratching, a follow-up study was undertaken. An SIV virus-positive, seropositive female with a normal menstrual cycle (used in the pilot study) was selected. The female showed signs of immunosuppression including CD4+ cell depletion, but was clinically healthy. A male, shown to be a proven breeder and determined to be SIV/SRV antibody-negative and virus-negative was given access to the female for two to three hours a day, three days a week, except when the female was menstruating. The interactions between the two animals were monitored by videotape and scored for duration and number of penile/vaginal penetrations. The video was also used to rule out other sources of transmission such as scratching or biting. The male was monitored for viremia and seroconversion every two weeks. The female was monitored every two weeks for any changes in viremia or antibody titer. Peripheral blood mononuclear cells (PBMC) were isolated on a Ficoll gradient and co-cultivated with the C8166 T cell line. In addition, polymerase chain reaction assay (PCR) was performed on PBMC and other lymphoid tissues. Other parameters monitored included lymphocyte subsets, body weight, temperature, lymphadenopathy, complete blood counts (CBC) and serum chemistries. The female's cervix was visually examined for ectopy prior to breeding and cervical swabs were collected monthly to assay for the presence of virus via immunofluorescence. Urethral swabs were also collected monthly for detection of viral antigen by immunofluorescence.
Keywords: Animal Antibodies, Viral/ANALYSIS Antigens, Viral/ANALYSIS Behavior, Animal Cervix Uteri/MICROBIOLOGY/PATHOLOGY Copulation Female Lymphocyte Subsets/IMMUNOLOGY Macaca nemestrina Male Polymerase Chain Reaction/METHODS Sexually Transmitted Diseases/*TRANSMISSION Simian Acquired Immunodeficiency Syndrome/BLOOD/IMMUNOLOGY/ *TRANSMISSION *SIV Urethra/MICROBIOLOGY ABSTRACT
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Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
