Dot blot to screen for antibodies to SRV-2 in the field. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Dot blot to screen for antibodies to SRV-2 in the field.

Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 111. Unique Identifier : AIDSLINE PRIM10/93200951
Welch MJ; Thouless ME; University of Washington Regional Research Primate Center,; Seattle.

Abstract: The establishment of an SRV-free breeding colony of M. nemestrina and M. fascicularis on Tinjil Island, Indonesia requires extensive testing of wild-caught macaques. The animals are currently held in quarantine for many weeks while their SRV status is determined by sensitive ELISA and virus culture tests. However a rapid prescreen, performed at the time of capture, could ensure that only macaques with negative preliminary results will be entered into the quarantine process. A dot blot test would be suitable for the difficult field conditions at the sites of wild macaque capture, because results can be obtained and interpreted without sophisticated equipment. To develop the dot blot, inactivated, solubilized membrane antigens were prepared from SRV-2 infected and uninfected A549 cells and were freeze-dried with preservatives. By Western blot, it was shown that gp70, p27, gp20, p14, p12 and p10 were present in the reconstituted SRV-2 antigen and absent in the uninfected cell antigen. The dot blot was optimized using these antigens with biotinylated anti-human IgG and streptavidin peroxidase conjugates. Results obtained with tests using positive and negative sera and whole blood from M. nemestrina will be presented.
Keywords: Animal Animals, Wild Antibodies, Viral/*BLOOD Blotting, Western/METHODS Cell Line Enzyme-Linked Immunosorbent Assay/METHODS Indonesia Macaca fascicularis/*MICROBIOLOGY Macaca nemestrina/*MICROBIOLOGY Quarantine Retroviruses Type D, Simian/*IMMUNOLOGY ABSTRACTKWDanimalanimals,wildantibodies,viral/KWDbloodblotting,western/methodscelllineenzyme-linkedimmunosorbentassay/methodsindonesiamacacafascicularis/KWDmicrobiologymacacanemestrina/KWDmicrobiologyquarantineretrovirusestyped,simian/KWDimmunologyabstract
930630
M9361069

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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