Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
In vitro cell proliferation study during vaccination of rhesus macaques with purified inactivated SIV.
Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 131. Unique Identifier : AIDSLINE PRIM10/93200974 Vaslin B; Le Grand R; Vogt G; Carlo D; Jensen F; Salk J; Dormont D; CEA/CRSSA, Fontenay aux Roses, France.
Abstract:
OBJECTIVES: To measure the state of helper T-cell activation in parallel with the humoral response after one, two or three injections of purified inactivated SIV delta emulsified in IFA to which cell wall skeleton (CWS) and monophospholipid (MPL) were added. METHODS: Two groups of 5 macaques were immunized with three 100 micrograms of purified SIV inactivated by 60Co gamma irradiation and beta propiolactone, on day 0, 56, 196. The first group (A) received the immunogen in an oil-in-water emulsion (RIBI Detox with CWS/MPL). A second group (B) received the immunogen in a water-in-oil emulsion (IFA with CWS/MPL). A third non-vaccinated group was used as control. Humoral responses were followed by mean of western blots and specific ELISA, neutralizing antibody and anti-cell antibody assays. PBMCs were stimulated in vitro with purified SIV. Dose dependence and kinetic of stimulation over time were studied. RESULTS: The humoral antibody responses of the B group were greater than those of the A group by at least 2 logs. Only animals in the B group had neutralizing antibodies. Proliferative responses in the B group became positive two weeks after the first injection. However, group A animals did not exhibit any lymphoproliferative responses even after the third dose. High stimulation was readily induced in the B group, reaching a peak index at 48 to 72 hours and plateauing at a dose as low as 0.03 microgram/ml. Specificity of the responses was confirmed by recombinant p27 antigen stimulation. These responses remained stable in vitro for at least two months after the third injection. Changes in stimulation indices over time will be shown. Despite high humoral and cellular response in group B animals, challenge of these monkeys with 10 to 100 AID50 of monkey PBL grown SIV mac251 resulted in infection of all animals. Virological data revealed higher levels of viremia in group B animals. The implications of these observations for SIV/HIV vaccine strategies will be discussed.
Keywords: Animal Antibodies, Viral/ANALYSIS Antibody Formation Blotting, Western Enzyme-Linked Immunosorbent Assay Gamma Rays *Lymphocyte Transformation Macaca mulatta Neutralization Tests Propiolactone/PHARMACOLOGY SIV/DRUG EFFECTS/*IMMUNOLOGY/RADIATION EFFECTS T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY Vaccines, Inactivated/*IMMUNOLOGY Viral Vaccines/*IMMUNOLOGY ABSTRACT 930630
M9361046
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Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
