1993

HIV testing : who, what, when, where, how. [videorecording]: Marshfield, WI : The Network, [1987] 1 videocassette (31 min.) : sd., col. ; 1/2 in (Medical education for 1987) Unique Identifier : AIDSLINE AV/9300606A; Corey D
Anorectal surgery in HIV-positive patients. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (15 min.) : sd., col. ; 1/2 in (NCME telecourse ; no. 626) Unique Identifier : AIDSLINE AV/9300529A; Smith LE; Provides diagnostic clues regarding anorectal lesions common in AIDS and AIDS-related disorders, and recommends a conservative approach to surgical intervention. Often minimal or no surgery is the best course of treatment in these infection-prone individuals who heal poorly.
Complications of high-risk pregnancy. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (49 min.) : sd., col. ; 1/2 in. + 1992 (NCME telecourse ; no. 622) Unique Identifier : AIDSLINE AV/9300535A; Fox HE; Baxi Ashar L; LaSala AP; Risk assessment in pregnancy must be a dynamic and interactive process. The physician must keep abreast of the more than 3000 identified risk factors, keep up to date with innovations in treatment and management, and be sensitive to the medical, psychosocial and emotional needs of the patient.
Contemporary immunization practices. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (16 min.) : sd., col. ; 1/2 in (NCME telecourse ; no. 630) Unique Identifier : AIDSLINE AV/9300536A; Irons TG; Schedules for routine and delayed childhood immunizations have significantly changed in recent years. In addition, new vaccine products have been licensed and recommended for use. Adult immunizations have also taken on new importance, particularly for individuals with HIV infection and foreign travelers. Dr. Irons out
Fundamentals of dermatologic surgery : skin grafts. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (10 min.) : sd., col. ; 1/2 in (NCME telecourse ; no. 626) Unique Identifier : AIDSLINE AV/9300544A; Leffell DJ; Discusses the factors which determine the choice of a full thickness graft, split thickness graft, or composite graft, then demonstrates a pinch graft to repair a wound following Mohs excision of a squamous cell carcinoma.
Identifying and managing elder abuse. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (20 min.) : sd., col. ; 1/2 in (NCME telecourse ; no. 626) Unique Identifier : AIDSLINE AV/9300549A; Fulmer TT; Lacks MS; Surveys confirm the extent of elder abuse and neglect and suggest that 35 per cent of all cases represent serious physical abuse. Program delineates risk factors, abuser and victim profiles, and techniques for recognition and intervention by physicians.
HIV disease update. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1992 1 videocassette (50 min.) : sd., col. ; 1/2 in + 1 booklet (NCME telecourse ; no. 625) Unique Identifier : AIDSLINE AV/9300547A; Witt DH; Hirschorn E; Highlights latest advances in therapeutics in the care of patients with HIV disease.
Tuberculosis in the age of AIDS. [videorecording]: Secaucus, N.J. : Network for Continuing Medical Education, 1993 1 videocassette (49 min.) : sd., col. ; 1/2 in. + 1 booklet (NCME telecourse ; no. 634) Unique Identifier : AIDSLINE AV/9300570A; Reichman LB; After a century of decline, the incidence of tuberculosis in the United States has steadily increased since 1985. Now the emergence of drug-resistant strains threatens our ability to control this preventable disease. Screening, diagnosing, and treating TB have also changed over the las
[Aids : forholdsregler mot smitte med HIV i laboratoriet.]: [Oslo?] : Helsedirektoratet, 1986 16 p (AIDS-skriv ; nr. 20) Unique Identifier : AIDSLINE CAT/9307508
Policy statement on AIDS : the role of the hospital social work department.: [Chicago, Ill.] : The Society, [1987] 7 p Unique Identifier : AIDSLINE CAT/9306140
Proceedings of WHO/NICD Workshop on Serosurveillance of AIDS, November 16-19, 1987.: Delhi : National Institute of Communicable Diseases, [1987?] 197 p Unique Identifier : AIDSLINE CAT/9307365; Kumari S; Rai A
AIDS : legal and regulatory policy.: Frederick, MD : University Pub. Group, c1988 viii, 381 p Unique Identifier : AIDSLINE CAT/9306662; Curran WJ; Gostin LO; Clark M
Nutrition for the AIDS carrier.: Saratoga, Calif. : R&E Publishers, c1989 56 p. : ill Unique Identifier : AIDSLINE CAT/9306114; Cox E
Health policy readings 1988 : a source book for health service administrators and students of health service administration.: [Sydney?] : Australian Hospital Association, [1989] (Canberra Calico Quick Print) 377 p. : ill., ports Unique Identifier : AIDSLINE CAT/9306362
AIDS and hemophilia : a coordinated education, support, and personal care program.: [Calgary?] : Calgary Region, Canadian Hemophilia Society, [1989] ix, 85 p Unique Identifier : AIDSLINE CAT/9306658; McDonald JR; Dowdle G
AIDS : information and guidelines for nurses.: Pretoria : Dept. of National Health and Population Development, [1989] 16 p Unique Identifier : AIDSLINE CAT/9306663
[AIDS : Orientierungshilfen fur das Bistum Limburg.]: Limburg/Lahn : Caritasverband fur die Diozese Limburg, [1989?] 52 p Unique Identifier : AIDSLINE CAT/9306664
The:Nurse and AIDS : legal issues.: Pretoria : The Association, 1989 15 p Unique Identifier : AIDSLINE CAT/9306892; Strauss SA
Proceedings of the First Southwest Regional Hispanic AIDS Conference, May 17-20, 1989.: San Antonio, Tex. : Hispanic AIDS Committee for Education and Resources, [c1989] iv, 360 p Unique Identifier : AIDSLINE CAT/9306937
Report of the working panel on aboriginals, Torres Strait islanders, and HIV/AIDS.: [Canberra] : Dept. of Community Services and Health, [1989] viii, 30 leaves (Consultation paper ; no. 1) Unique Identifier : AIDSLINE CAT/9306957
Report of the working panel on testing for HIV/AIDS.: [Canberra] : Dept. of Community Services and Health, c1989 vii, 20 p (Consultation paper ; no. 5) Unique Identifier : AIDSLINE CAT/9306958
Report of the working panel on treatment, services and care, HIV/AIDS.: [Canberra] : Dept. of Community Services and Health, c1989 vii, 28 p (Consultation paper ; no. 6) Unique Identifier : AIDSLINE CAT/9306959
Financing care for persons with AIDS : the first studies, 1985-1988.: Frederick, MD : University Pub. Group, c1989 ix, 357 p Unique Identifier : AIDSLINE CAT/9307178; Fox DM; Thomas EH
[Therapeutische Wege : eine Orientierungshilfe fur anti-HIV-positive Menschen.]: Wien : Promedia, c1989 87 p Unique Identifier : AIDSLINE CAT/9307900; Vavrik K
Planning for HIV/AIDS care and treatment services in New South Wales, 1990-1994 : report.: North Sydney, NSW : NSW Health Dept., c1990 193 p. : ill (State health publication ; (AIDS) 90-68) Unique Identifier : AIDSLINE CAT/9306139
[L':ultima epidemia : la guerra batteriologica, dalla peste all'AIDS?.]: S. Domenico di Fiesole : Cultura della pace, c1990 205 p., [16] p. of plates. : ill (Uomo planetario ; 3) Unique Identifier : AIDSLINE CAT/9306240; Santoianni F
[Aids : de jacht op een virus.]: Maastricht : Natuur & techniek, c1990 148 p : ill., ports Unique Identifier : AIDSLINE CAT/9306246; Rozendaal S
Human sexuality : research perspectives in a world facing AIDS : background papers and reports of a workshop held at International Development Research Centre, Ottawa, Ontario, Canada, June 1989.: Ottawa : The Centre, [1990] iii, 210 p Unique Identifier : AIDSLINE CAT/9306377; Chouinard A; Albert J
Common oral lesions associated with HIV infection.: Columbus, Ohio : Ohio State University, c1990 29 p. : ill Unique Identifier : AIDSLINE CAT/9306702; Haring JI
Key issues in HIV infection and AIDS for Victorian GPs.: Melbourne : AIDS/STD Unit, Health Dept., Victoria, 1990 11 p Unique Identifier : AIDSLINE CAT/9307715
[SIDA : una enfermedad de nuestro tiempo.]: [Chile?] : Monte Grande, 1990 132 p. : ill Unique Identifier : AIDSLINE CAT/9307894; Zenteno Pardo M
Homophobia, heterosexism, and AIDS : creating a more effective response to AIDS.: Ottawa : Canadian AIDS Society, 1991 68 p Unique Identifier : AIDSLINE CAT/9306022
Common sense about AIDS.: [Atlanta, Ga.?] : American Health Consultants, v Unique Identifier : AIDSLINE CAT/9306288
[Rad og veiledning til hiv-positive.]: Oslo : Oslo Kommune, Etat for miljorettet helsevern, 1991 52 p. : ill Unique Identifier : AIDSLINE CAT/9306517; Muniz HP
Training drug treatment staff in the age of AIDS : a frontline perspective.: Rockville, Md. (5600 Fishers Ln., Rockville 20857) : U.S. Dept. of Health and Human Services, Public Health Service, Alcohol, Drug Abuse, Mental Health Administration, National Institute on Drug Abuse, Division of Applied Research, Community Research Branch, [1991] v, 36 p (Clinical report series) Unique Identifier : AIDSLINE CAT/9306558; Ashery RS
AIDS in context : a South African perspective. 1st ed.: Cape Town : Lux Verbi, 1991 111 p Unique Identifier : AIDSLINE CAT/9306660; Van Niekerk AA
Differential diagnosis in AIDS : a color guide.: St. Louis : Mosby Year Book, c1991 159 p. : ill Unique Identifier : AIDSLINE CAT/9306726; Parkin JM; Peters BS
HIV counselor perspectives. Vol. 1, no. 1 (Jan. 1991)-: Sacramento, CA : California Dept. of Health Services, State Office of AIDS, [1991- v. : ill Unique Identifier : AIDSLINE CAT/9306795
HIV/AIDS and prisons : proceedings of a conference held 19-21 November 1990.: Canberra, ACT : Australian Institute of Criminology, c1991 296 p. : ill (Conference proceedings series, ISSN 1034-5086 ; no. 4) Unique Identifier : AIDSLINE CAT/9306796; Norberry J
Men who have sex with men in Australia : AIDS : a report on the Gammaline telephone counselling service.: [Australia : s.n., 1991?] 1 v. (unpaged) : ill Unique Identifier : AIDSLINE CAT/9306863; Palmer WA
Preventing HIV infection among youth.: Rockville, MD (5600 Fishers Ln. (Rockwall II), Rockville 20857) : U.S. Dept. of Health and Human Services, Public Health Service, Alcohol, Drug Abuse, and Mental Health Administration, Office for Substance Abuse Prevention, [1991] vii, 41 p (OSAP technical report ; 5) Unique Identifier : AIDSLINE CAT/9306935; Goplerud EN; Resnik H
AIDS : information for physicians.: Harrisburg, Pa. (777 East Park Dr., Harrisburg 17105-8820) : the Society, [1991] 40 p Unique Identifier : AIDSLINE CAT/9307107
Florence conference update : HIV/AIDS surveillance data base.: Washington, D.C. : Center for International Research, U.S. Bureau of the Census, [1991] 43, 8 leaves Unique Identifier : AIDSLINE CAT/9307180
[Vrouwen en AIDS : advies.]: Amsterdam : Nationale Commissie AIDS-Bestrijding, 1991 63 p. : ill Unique Identifier : AIDSLINE CAT/9307455
State of Alaska AIDS Program.: Anchorage, Alaska (3601 C St. Suite 576, P.O. Box 240249, Anchorage 99524-0249) : Section of Epidemiology, Division of Public Health, Dept. of Health and Social Services, State of Alaska, [1991] 22 p. : ill Unique Identifier : AIDSLINE CAT/9307884
Drug addiction treatment research : German and American perspectives.: Malabar, Fla. : Krieger Pub. Co., 1992 xxii, 528 p. : ill Unique Identifier : AIDSLINE CAT/9306313; Buhringer G; Platt JJ
Ethics and law in the study of AIDS.: Washington, D.C., U.S.A. : Pan American Health Organization, Pan American Sanitary Bureau, Regional Office of the World Health Organization, c1992 x, 273 p (Scientific publication ; no. 530) Unique Identifier : AIDSLINE CAT/9306328; Fuenzalida-Puelma HL; Linares Parada AM; Serrano La Vertu D
The:role of high-risk occupations in the spread of AIDS : truck drivers and itinerant market women in Nigeria.: [Canberra] : Australian National University, [1992] 11 p (Health Transition working paper ; no. 10 (1992)) Unique Identifier : AIDSLINE CAT/9306599; Orubuloye IO
Advances on the AIDS horizon : 1992. 6th ed.: Sacramento, Calif. : R.D. Anderson Pub. Co., c1992 87 p Unique Identifier : AIDSLINE CAT/9306625; Walmer M
AIDS and primary health care : the role of non-governmental organizations.: Amsterdam : VU University Press, 1992 77 p. : ill (Primary health care publications ; 4) Unique Identifier : AIDSLINE CAT/9306659; Wolffers I
AIDS in Australia.: New York : Prentice Hall, c1992 xxiv, 392 p Unique Identifier : AIDSLINE CAT/9306661; Timewell E; Minichiello V; Plummer D
Cytokines in hemopoiesis, oncology, and AIDS II.: Berlin ; New York : Springer-Verlag, c1992 li, 800 p. : ill Unique Identifier : AIDSLINE CAT/9306712; Freund M
[Das:Sexualverhalten Jugendlicher unter der Bedrohung von AIDS.]: Berlin : Berlin Verlag Arno Spitz, c1992 iii, 231 p (Berlin Forschung ; Bd. 26) Unique Identifier : AIDSLINE CAT/9306982; Mischnick H
AIDS/HIV infection and the workplace : NIDA workgroup report. Repr. 1992.: [Rockville, Md.] : Dept. of Health and Human Services, Public Health Service, Alcohol, Drug Abuse, and Mental Health Administration ; Washington, DC : For sale by the U.S. G.P.O., Supt. of Docs., 1992 viii, 36 p. : ill (DHHS publication ; no. (ADM) 92-1716) Unique Identifier : AIDSLINE CAT/9307108
AIDS in the industrialized democracies : passions, politics, and policies.: New Brunswick, N.J. : Rutgers University Press, c1992 xi, 393 p Unique Identifier : AIDSLINE CAT/9307539; Kirp DL; Bayer R
[L'Education sexuelle au temps du SIDA.]: Toulouse : Privat, c1992 190 p (Pratiques sociales) Unique Identifier : AIDSLINE CAT/9307727; Ruffiot A
Modeling HIV transmission and AIDS in the United States.: Berlin ; New York : Springer-Verlag, c1992 xi, 234 p. : ill (Lecture notes in biomathematics ; 95) Unique Identifier : AIDSLINE CAT/9307767; Hethcote HW; Van Ark JW
[Vejledning om human immundefekt virus HIV og forebyggelse af blodbaren smitte.]: [Kobenhavn?] : Sundhedsstyrelsen, 1992 86 p. : ill Unique Identifier : AIDSLINE CAT/9307921
Interaction of the human T cell leukemia virus-I transactivator protein Tax with the host cell transcriptional machinery.: Diss Abstr Int [B]; 53(6):2692 1992. Unique Identifier : AIDSLINE ICDB/93690097; Matthews MH; Univ. of Colorado at Boulder; The Tax protein, encoded by human T cell leukemia virus-I (HTLV-I), is a potent transactivator of transcription. Because several of the genes transactivated by Tax are involved in regulation of cell growth, it is thought that transactivation may contribute to viral oncogenesis. Unlike a number of other viral oncogenes
Treatment of lymphomas with 131I-Lym-1 (Meeting abstract).: Fourth Annual Symposium: Current Status and Future Directions of Immunoconjugates. Diagnostic and Therapeutic Applications in Benign and Malignant Disorders. January 23-26, 1992, Key Biscayne, FL, p. 68, 1992.. Unique Identifier : AIDSLINE ICDB/93690321; DeNardo GL; Univ. of California Davis, Molecular Cancer Inst., 1508 Alhambra; Blvd., Sacramento, CA 95816; The development of monoclonal antibodies has stimulated new interest in their use for specific targeting as vehicles for the diagnosis and treatment of cancer. While this approach is analogous to the use of chemotherapy, it has the advantage of specific targeting to the cancer. While targeting of all of the antibody t
Localization and characterization of transforming regions in human cytomegalovirus and human herpesvirus 6.: Diss Abstr Int [B]; 53(7):3300 1993. Unique Identifier : AIDSLINE ICDB/93690162; Thompson JT; Georgetown Univ. Medical Center; Human herpesviruses have been associated with the etiology of several human cancers. The role these viruses play in carcinogenesis has not been delineated. To elucidate the role of human herpesviruses in carcinogenesis, in vitro model systems have been developed which have identified multiple DNA fragments capable of
Pathogenesis of SIVmac251 after atraumatic inoculation of the rectal mucosa in rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 1. Unique Identifier : AIDSLINE PRIM10/93200939; Pauza CD; Emau P; Salvato MS; Trivedi P; Mackenzie D; Malkovsky M; Schultz KT; Wisconsin Regional Primate Research Center, University of; Wisconsin-Madison 53706.; One year old juvenile male rhesus monkeys were infected with SIVmac251 via atraumatic inoculation of the rectal mucosa. Virus doses ranging from 1,000 to 0.1 tissue culture infectious doses (determined on CEMx174 cells) established infection in all animals. Three of four animals receiving 1,000 or 100 doses became ser
Biology of acute infection with macrophage-tropic SIVmac.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 10. Unique Identifier : AIDSLINE PRIM10/93200940; Ringler D; Mori K; Sasseville V; Walsh D; Pauley D; Hesterberg P; Daniel M; Desrosiers R; Division of Pathology, Harvard Medical School, Southborough, MA.; We previously demonstrated that molecularly-cloned SIVmac239 replicates in lymphoid cells and induces AIDS in rhesus macaques, yet replicates poorly in simian alveolar macrophage cultures. In some SIVmac239-infected animals, macrophage-tropic variants of SIVmac239 arise in vivo and can be isolated from the peripheral
Characterization of AZT susceptibility of biological SIVmac and molecular clones, and emergence of AZT resistant mutants in vitro.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 108. Unique Identifier : AIDSLINE PRIM10/93200941; Van Rompay KK; Marthas ML; Lohman BL; Pedersen NC; California Regional Primate Research Center, University of; California, Davis 95616.; BACKGROUND: It is not known whether AZT resistant HIV mutants, which emerge in HIV following long-term AZT therapy, have altered pathogenicity. Therefore, it is still controversial whether or not to discontinue AZT administration in these people. Beca
Correlation of immunological, clinical, neuropathological, and neurochemical and neurochemical parameters with motor and cognitive impairments observed in SIV-infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 103. Unique Identifier : AIDSLINE PRIM10/93200942; Rausch DM; Murray EA; Lendvay J; Sharer LR; Heyes M; Nohr D; Weihe E; Eiden LE; National Institute of Mental Health Bethesda, MD.; A group of rhesus macaques infected with SIVB670-Delta exhibited variegated cognitive and motor impairments as described in some HIV-infected humans (Murray et al., Science 255:1246, 1992). In each case, CNS impairments preceded signs of SIV-induced illness. Blood and CSF samples taken throughout the course of the stu
Effect of dosing frequency on AZT prophylaxis against SIV infection in cynomolgus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 107. Unique Identifier : AIDSLINE PRIM10/93200943; Tsai CC; Follis KE; Grant R; Nolte RE; Bartz C; Benveniste RE; Sager PR; Regional Primate Research Center, University of Washington,; Seattle 98195.; Early AZT treatment has been shown to prolong life for certain HIV-infected persons if taken before the symptoms of AIDS develop. However, it is still uncertain whether dosing frequency of AZT affects anti-HIV activity. Therefore, we studied the effec
In vivo tracking of lymphocytes in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 105. Unique Identifier : AIDSLINE PRIM10/93200944; Salvato M; Johnson E; Rater M; Pauza CD; Dept. of Pathology and Lab. Medicine, Wisconsin Regional Primate; Research Center, Madison 53706.; Our goal is to develop lymphocyte replacement therapy in SIV-infected rhesus macaques. Pilot studies to determine the efficacy of storing, culturing and re-introducing lymphocytes monitored the distribution and half-life of re-introduced lymphocyte cultures. Lymphocytes were isolated from rhesus monkeys and marked eit
Longitudinal evaluation of immune dysfunction in SIV-infected rhesus macaques, monitored by a novel tri-parametric immunological staging system.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 100. Unique Identifier : AIDSLINE PRIM10/93200945; Lavergne JA; Del Llano AM; Amieiro JP; Kraiselburd EN; Kessler MJ; Malaga C; Department of Microbiology, University of Puerto Rico, San Juan; 00936-5067.; A novel immunological staging system (ISS) based on three in vitro parameters of immune function (blood cell apoptosis, mitochondrial metabolism and proliferative response to PWM) was utilized for the longitudinal follow-up of a group of eight SIV-infected rhesus macaques, during fifteen months after their infection w
Molecular characterization of STLV-1-positive lymphomas in baboons (Papio hamadryas).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 109. Unique Identifier : AIDSLINE PRIM10/93200946; Voevodin AF; Shields P; Klotz IN; Kutitova OK; Tsargush AS; Institute of Pulmonology, Moscow, Russia.; Epizootiology, pathology, virology and immunology of malignant lymphomas in baboons (Papio hamadryas) were described earlier (for review see B. Lapin in: Viral Diseases in Laboratory and Captive Animals, Ed. G. Darai, 1988, p. 135). A strong, presumably aetiological linkage was found between these lymphomas and the HT
Non-sexual horizontal transmission of SIVSMM.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 101. Unique Identifier : AIDSLINE PRIM10/93200947; McClure HM; Novembre FJ; Hirsch VM; Lockwood E; Saucier M; Hamm T; Johnson PR; Yerkes Primate Research Center, Emory University, Atlanta, GA; 30322.; The mode of transmission of SIV among nonhuman primates has not been fully documented, although studies have suggested sexual transmission, mother-to-infant transmission, and non-sexual horizontal transmission from bites. As information of this type is needed for colony management and to determine the type of housing
Response to superantigens in HIV-1 and HIV-2 infected nonhuman primates.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 102. Unique Identifier : AIDSLINE PRIM10/93200948; Murthy KK; Guevara SM; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, TX 78228.; Superantigens are bacterial endotoxins that act as potent stimulators of T cells from mice and man. According to recent studies, proliferative response to superantigens is depressed in HIV-1 infected humans, presumably due to deletion of T cell clones mediated by viral infection. Therefore, we evaluated the response o
Transmission of SIVMne in adult Macaca nemestrina.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 106. Unique Identifier : AIDSLINE PRIM10/93200949; Thompson J; Kuller L; Watanabe R; Firpo P; Morton WR; Regional Primate Research Center, University of Washington,; Seattle 98195.; We have previously reported results of a pilot study which indicated the heterosexual transmission of the simian immunodeficiency virus (SIVMne) from female to male pigtailed macaques (M. nemestrina). To better quantify this mode of transmission and to exclude other factors of transmission such as biting and scratchin
Characterization of SIV envelope proteins derived from SIVmac infected macaques shortly after infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 117. Unique Identifier : AIDSLINE PRIM10/93200950; Hulskotte EG; Rimmelzwaan GF; Bosch ML; de Vries P; Heeney JL; Osterhaus AD; Laboratory of Immunobiology, RIVM, Bilthoven, The Netherlands.; We have shown that vaccination with an ISCOM preparation based on envelope protein enriched whole SIVmac is effective against subsequent challenge with the homologous strain of SIV. It is of interest to know whether envelope protein alone incorporated into ISCOMs would also be able to confer protection against SIV cha
Dot blot to screen for antibodies to SRV-2 in the field.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 111. Unique Identifier : AIDSLINE PRIM10/93200951; Welch MJ; Thouless ME; University of Washington Regional Research Primate Center,; Seattle.; The establishment of an SRV-free breeding colony of M. nemestrina and M. fascicularis on Tinjil Island, Indonesia requires extensive testing of wild-caught macaques. The animals are currently held in quarantine for many weeks while their SRV status is determined by sensitive ELI1
Human cell-grown HIV-1 vaccine protects macaques against challenge with SIVmac.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 113. Unique Identifier : AIDSLINE PRIM10/93200952; Cranage MP; Polyanskaya N; McBride B; Cook N; Greenaway P; Dennis M; Ashworth T; Farrar G; Division of Pathology, PHLS Centre for Applied Microbiology &; Research, Porton Down, Salisbury, U.K.; The recent observations that protection against SIVmac challenge correlates with anti-cell antibody responses, that cells alone can elicit a degree of anti-viral protection and that protection is limited to challenge stocks grown in human cells, has lead to a re-evaluation of the role of anti-cell antibodies in SIV va1
Immune enhancement and attenuation of vaccinia virus recombinants expressing empty viral particles of SIV and lymphokines.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 115. Unique Identifier : AIDSLINE PRIM10/93200953; Giavedoni LD; Parodi L; Ahmad S; Bassiri M; Yilma T; International Laboratory of Molecular Biology for Tropical; Disease Agents, University of California, Davis 95616.; Simian immunodeficiency virus (SIV) infection in rhesus macaques is a model for human immunodeficiency virus (HIV) in humans. Limited protection against SIV has been demonstrated in rhesus macaques vaccinated with live attenuated or inactivated viral vaccine. However, the use of whole inactivated or live, virus vaccin1
Immunization of rhesus macaques by lipopeptides induces cytolytic T lymphocytes (CTL).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 112. Unique Identifier : AIDSLINE PRIM10/93200954; Bourgault I; Venet A; Levy JP; ICGM, U 152, Hopital Cochin, Paris, France.; INTRODUCTION: An effective vaccination against HIV/SIV will require the generation of a CTL response in addition to neutralizing antibodies in order to kill early infected cells. After determining that NEF and GAG proteins were highly immunogenic for induction of CTL in SIVmac251-infected macaques (J. Immunol. 1992; 11
Infectious SRV in excreta of viremic M. nemestrina?: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 110. Unique Identifier : AIDSLINE PRIM10/93200955; Welch MJ; Moazed TC; Morton WR; University of Washington Regional Research Primate Center,; Seattle.; SRV is a naturally occurring type D retrovirus which causes disease in macaque species. After exposure to SRV, most macaques become nonviremic seropositive and show no clinical signs of SRV infection. However, a small number become persistently viremic, displaying only mild clinical signs of SRV infection. Previous st1
Pre-challenge neutralization titers reflect post-challenge virus load in HIV-1 vaccinated chimpanzees.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 116. Unique Identifier : AIDSLINE PRIM10/93200956; Heeney J; Thiriart C; Koornstra W; Dubbes R; ten Haaft P; Delers A; Fabry L; Francotte M; von Opstal O; Culp J; et al; Laboratory of Viral Pathogenesis, ITRI-TNO, Rijswijk, The; Netherlands.; A quantitative virus isolation assay was developed for the determination of virus load in HIV-1 infected chimpanzees. In a study designed to study immunological correlates of HIV-1 vaccine protection, two different recombinant HIV-1 envelope preparations were used. Recombinant forms of HIV-1 env gp160 and gp120 (III B1
Production of genetically inactivated SIVsmmPBj14 viruslike particles for use as a potential vaccine.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 114. Unique Identifier : AIDSLINE PRIM10/93200957; Deschamps M; Horth M; Kummert S; Gelderblom H; Thiriart C; Bruck C; Burny A; Laboratory of Biological Chemistry, Free University of Brussels,; Rhode-St-Genese, Belgium.; We have recently established a stable Vero cell line that produces genetically inactivated SIVsmPBj1.9 (molecular clone of SIVsmmPBj14) viral particles that are very similar to the native virus and that are non-infectious in vitro. The SIVsmmPBj1.9 provirus deleted of its 3 LTR, was expressed in Vero cells in stable t1
SIV host range specificity transfer to recombinant HIV-1 virions via SIV envelope glycoproteins.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 11. Unique Identifier : AIDSLINE PRIM10/93200958; Johnston PB; Hunter E; Department of Microbiology, University of Alabama, Birmingham; 35294.; The host range specificity of simian immunodeficiency virus, SIVmac239, appears to be modulated by the cytoplasmic domain of its envelope glycoprotein. Propagation of SIVmac239 in HUT78 cells results in truncation of the transmembrane (TM) protein to 207-211 amino acids. We sought to determine whether further truncati1
Vaccination against SIV infection with subunit proteins vs inactivated virus.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 118. Unique Identifier : AIDSLINE PRIM10/93200959; Israel ZR; Maul DH; Bruck C; Thiriart C; Mullins JI; Fultz PN; Burny A; Hoover EA; Department of Pathology, Colorado State University, Fort Collins; 80523.; The goal of this work was to evaluate the efficacy of immunization with either env or env+gag proteins vs. whole inactivated virus prepared from SIVmac251 clone BK28. Twenty three juvenile M. mulatta were placed into 6 vaccine groups of 3 macaques each as follows: (1) WIV boosts after priming with live intradermal VV 1
Vaccine studies in rhesus macaques using whole inactivated SIVMAC immunogen.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 119. Unique Identifier : AIDSLINE PRIM10/93200960; Kurth R; Hartung S; Konig H; Ennen J; Cichutek K; Jensen F; Norley S; Paul-Ehrlich-Institut, Langen, FRG.; Eight rhesus macaques were immunized intramuscularly four times (0, 1, 2, 4 months) with 0.5 mg or 0.1 mg of formalin inactivated whole SIV immunogen in the presence of muramyl dipeptide (MDP). After intravenous challenge with 50 MID50 of SIVmac251-32H, three of the four animals from the low dose group (plus the nonva1
Cryptic neutralizing epitopes on SIVagm gp120; role in viral entry.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 12. Unique Identifier : AIDSLINE PRIM10/93200961; Allan JS; Whitehead E; Strout K; Dunham R; Kanda P; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas 78228.; We previously showed that addition of soluble CD4 to SIVagm viruses leads to the enhanced kinetics of infection and cytopathology in human cell lines. We have termed this process receptor-mediated activation. Recent evidence also suggests that HIV-1 and HIV-2 also undergo conformational changes associated with recepto1
Efficiency of a subunit vaccine consisting of GP130 micelles in protection of rhesus monkeys against challenge with SIVmac251/32H.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 122. Unique Identifier : AIDSLINE PRIM10/93200962; Luke W; Voss G; Dittmer U; Coulibaly C; Stahl-Hennig C; Petry H; Hunsmann G; German Primate Center, Gottingen.; Three rhesus monkeys were immunized four times four weeks apart with a total of 400 g of gp130 micelles per animal, mixed with KLH and emulsified in incomplete Freund s adjuvant. All immunized animals have developed antibody titers of about 10(5) against gp130. Neutralizing and CD4-binding inhibiting antibodies could 1
Immunization of cynomolgus macaques with the envelope glycoprotein gp130 of HIV-2ben confers protection against the homologous but not the heterologous virus challenge with HIV-2SBL-6999.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 123. Unique Identifier : AIDSLINE PRIM10/93200963; Luke W; Voss G; Stahl-Hennig C; Coulibaly C; Petry H; Hunsmann G; German Primate Center, Gottingen.; Nine cynomolgus monkeys were immunized over a period of 52 weeks with gp130 of HIV-2ben mixed with KLH containing 1.1 mg of purified gp130 per animal. For the first 3 immunizations we used incomplete Freund s adjuvant. Because of severe inflammatory reactions antigen was adsorbed to aluminium hydroxide for the later 41
Immunization of rhesus monkeys with split and subunit vaccines of SIVagmTYO-7.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 121. Unique Identifier : AIDSLINE PRIM10/93200964; Luke W; Stahl-Hennig C; Wyand MS; Manson K; Petry H; Voss G; Hunsmann G; German Primate Center, Gottingen.; Three rhesus monkeys were immunized with gp130 micelles linked to KLH three times four weeks apart with a total of 60 micrograms of gp130 in incomplete Freund s adjuvant. Three additional rhesus monkeys were vaccinated with a Tween-Ether (TE) extract of purified SIVagmTYO-7 following the same immunization schedule as 1
Immunogenicity of SIV gp120 and gp41 envelope proteins expressed as hybrid Ty-VLPs.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 129. Unique Identifier : AIDSLINE PRIM10/93200965; Stasiak P; Stenner N; Stabler G; Kent K; Almond N; Kitchin P; Mills K; Layton G; Adams S; Kingsman A; British Bio-technology Ltd., Oxford, U.K.; SIV infection of macaques has been developed as a model for AIDS resulting from HIV infection of humans. By analogy with HIV, it is likely that the envelope protein of SIV will play an important role in eliciting neutralising, and possibly protective, responses. It is therefore important to define epitopes within the 1
Modification of viral load after immune stimulation of SIV-infected rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 120. Unique Identifier : AIDSLINE PRIM10/93200966; Le Grand R; Kress JP; Vogt G; Vaslin B; Roques P; Theodoro F; Aubertin AM; Dormont D; CEA/CRSSA, Fontenay aux Roses, France.; OBJECTIVES: To assess the influence of adjuvant injection on the clinical status and on the seric and PBMC viral loads of SIV infected monkeys. METHODS: Two groups of two rhesus monkeys were injected intravenously with cell free SIVmac251 or SIVsm, respectively three and one year before they were injected with 1 ml of1
Molecular cloned SIVmac32H vaccine and challenge virus.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 127. Unique Identifier : AIDSLINE PRIM10/93200967; Rud EW; Yon JR; Quirk J; Corbin AY; Clarke BE; Mackett M; Stott EJ; Kent KA; Corcoran T; Cook N; et al; Wellcome Research Laboratories, Dept. of Mol. Sci., Beckenham,; Kent, U.K.; We molecularly cloned, sequenced and titrated genomic clones of the SIVmac32H vaccine challenge virus used by the British MRC AIDS Directed Programme. The env gene was subcloned out of the full length molecular clone, SIVmac32H(pJ5) and inserted into various expression vectors. The molecular clones of SIVmac32H were s1
Passive immunization and challenge of rhesus macaques with SIVMAC.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 126. Unique Identifier : AIDSLINE PRIM10/93200968; Rosenthal A; Jennings MB; Cotterman R; Schmerl S; Yee JL; Joye S; Gardner MB; Dept. of Pathology, School of Medicine, University of California,; Davis.; 14 monkeys were infused with purified immunoglobulin (Ig) or heat inactivated plasma prior to inoculation with 10-100 I.D. 50 of cell-free SIVMAC. 4 of 8 animals infused with plasma from an SIV-immunized animal were protected against infection. None of the 6 animals that were infused with plasma or Ig from infected an1
Passive protection of neonatal rhesus monkeys born of mothers immunized with whole inactivated SIV.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 125. Unique Identifier : AIDSLINE PRIM10/93200969; Murphey-Corb M; Davison-Fairburn B; Ohkawa S; Martin L; Tulane Primate Center, Covington, LA 70433.; Four pregnant female rhesus monkeys were immunized with three 100 micrograms doses of formalin-inactivated SIV + SAF-M during pregnancy, with the final immunization given 2-4 weeks prior to delivery. One infant was born prematurely due to an incompetent cervix in the mother and died. The remaining 3 infants were deliv1
Simian antibody responses to linear and conformational epitopes of SIV envelope following vaccination or infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 128A. Unique Identifier : AIDSLINE PRIM10/93200970; Silvera P; Flanagan B; Kent K; Powell C; Stott J; National Institute for Biological Standards and Control, Potters; Bar, Herts, UK.; Cynomolgus macaques were infected with SIV or vaccinated with one of three different recombinant envelope proteins. Their antibody responses were quantitated by ELISA using, as antigens, native virus, recombinant proteins or overlapping peptides analogous to the entire envelope protein. Plasma were also examined for a1
Spectrum of neutralizing sera in macaques immunized with either HIV-1B RU/HBsAg or HIV-1 MN/HBsAg hybrid particles.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 128. Unique Identifier : AIDSLINE PRIM10/93200971; Schlienger K; Mancini M; Riviere Y; Tiollais P; Michel ML; Unite de Recombinaison et Expression Genetique, Institut; Pasteur, Paris, France.; Hepatitis B surface antigen (HBsAg) produced by recombinant DNA technology is now largely and safely used for worldwide hepatitis B vaccination. We recently reported that recombinant HIV/HBsAg particles presenting the HIV-1 BRU envelope principal neutralizing determinant (PND) on their surface allowed us to generate p1
Detection of cellular antibodies in SIV immunized monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 132. Unique Identifier : AIDSLINE PRIM10/93200972; Yee JL; Jennings MB; Acevedo R; Gardner MB; Department of Medical Pathology, University of California, Davis.; Understanding the specificity and function of antibodies to cells and/or cellular components in immunized monkeys is important in vaccine development. In a retrospective study using frozen pre- and post-immunization sera from monkeys immunized with various inactivated SIVmac (grown in human cell lines)/adjuvant combin1
Immunization of Macaca fascicularis with inactivated SIV preparations: a) challenge with human- or monkey-derived-SIV; b) effects of longer immunization schedule.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 130. Unique Identifier : AIDSLINE PRIM10/93200973; Titti F; Koanga Mogtomo L; Borsetti A; Geraci A; Sernicola L; Panzini G; Turillazzi GP; Pace M; Baroncelli S; Zamarchi R; et al; Istituto Superiore di Sanita, Rome, Italy.; OBJECTIVE: To evaluate i) the efficacy of two different vaccine preparations and schedules of immunization, ii) whether there is a different protection against human (32H) or monkey-derived-SIV, iii) the role of anti-cellular antibodies in protective immunity. RESULTS: 5 males Macaca fascicularis (Mf) (group A) were i1
In vitro cell proliferation study during vaccination of rhesus macaques with purified inactivated SIV.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 131. Unique Identifier : AIDSLINE PRIM10/93200974; Vaslin B; Le Grand R; Vogt G; Carlo D; Jensen F; Salk J; Dormont D; CEA/CRSSA, Fontenay aux Roses, France.; OBJECTIVES: To measure the state of helper T-cell activation in parallel with the humoral response after one, two or three injections of purified inactivated SIV delta emulsified in IFA to which cell wall skeleton (CWS) and monophospholipid (MPL) were added. METHODS: Two groups of 5 macaques were immunized with three 1
Recombinant SIVgp160 does not induce protective immunity against experimental infection of rhesus macaques with SIV.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 133. Unique Identifier : AIDSLINE PRIM10/93200975; Yilma T; Ahmad S; Giavedoni L; el-Amad Z; Scandella C; Haigwood N; Marthas M; Gardner M; Luciw P; University of California, Davis 95616.; Simian immunodeficiency virus (SIV) infection of rhesus macaques is a model for human immunodeficiency virus (HIV) in humans. Inactivated and modified live, whole-virus vaccines have provided limited protective immunity against SIV in rhesus macaques. Because of safety concerns in the use of inactivated or live, whole1
The combined assessment of cellular apoptosis, mitochondrial function and proliferative response to PWM has predictive value in SIV infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 13. Unique Identifier : AIDSLINE PRIM10/93200976; Del Llano AM; Lavergne JA; Department of Microbiology, University of Puerto Rico, San Juan; 00936-5067.; Routine monitoring of SIV-infected macaques includes the assessment of clinical parameters of disease progression, such as persistent lymphadenopathy, diarrhoea or weight loss. However, these clinical signs usually become evident only after significant immunologic impairment has already occurred in the animal. The nee1
Update of HIV and SIV challenge/protection studies in vaccinated nonhuman primates: progress in first and second order studies.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 131A. Unique Identifier : AIDSLINE PRIM10/93200977; Warren JT; Dolatshahi M; EMMES Corporation, Potomac, Maryland 20854.; We maintain an international database (the AIDS Vaccine Surveillance System, AVSS) that tracks the rapidly growing number of preclinical nonhuman primate (NHP) AIDS vaccine/live virus challenge studies (Warren and Dolatshahi, J Med Primatol 21:139-186, 1992). Summary data on more than 100 preclinical HIV/SIV vaccines 1
A cytotoxic T lymphocyte epitope shared between HIV-2 and SIV.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 14. Unique Identifier : AIDSLINE PRIM10/93200978; Gotch F; Gallimore A; Whittle H; McMichael A; Institute of Molecular Medicine, Oxford, U.K.; It has been reported that some strains of HIV-2 are more closely related to SIVsm/SIVmac virus than to any known virus of human derivation. HIV-2 and SIV can cause immunodeficiency, manifested by a reduction in the number of CD4+ lymphocytes, but both viruses are thought to be less pathogenic than HIV-1. Experimental 1
Factors influencing initial infection of resting T-cells with simian immunodeficiency virus.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 15. Unique Identifier : AIDSLINE PRIM10/93200979; Firpo PP; Liang A; Clark E; University of Washington, Primate Center, Seattle.; Both HIV and SIV are highly tropic for cells expressing the CD4 receptor. After viral binding to CD4 and entry of virus into host cells, viral DNA is synthesized in the cytosol and eventually integrated into the host genome. We have previously reported that unstimulated or activated T cells have detectable SIV-DNA 24 1
A neutralization epitope on the surface envelope glycoprotein (gp130) of simian immunodeficiency virus (SIVmac).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 16. Unique Identifier : AIDSLINE PRIM10/93200980; Torres JV; Malley A; Banapour B; Anderson DE; Benjamini E; Gardner MB; Department of Medical Microbiology and Immunology, School of; Medicine, University of California, Davis 95616.; SIVmac infection of macaques is an important animal model for human AIDS that is being used for development of antiviral therapies and vaccines. For the effective use of this model in vaccine design and testing, we need to acquire a better understanding of the molecular details concerning the immune response to retrov1
Nucleotide changes in the envelope gene of SIVmac239 during neuroadaptation in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 17. Unique Identifier : AIDSLINE PRIM10/93200981; Anderson MG; Hauer DA; Joag SV; Narayan O; Clements JE; Div. Comparative Med., Johns Hopkins University School of; Medicine, Baltimore, MD 21205.; An infectious molecular clone of SIVmac239, which is highly T-cell-tropic and grows poorly in monocytes and macrophages in vitro, maintained its T-cell tropism in infected animals (Kestler et. al., 1990; Naidu et. al., 1988; Sharma et. al., 1991). SIVmac239 infected animals developed AIDS, their macrophages did not be1
Molecular basis of SIVmac neuropathogenesis.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 18. Unique Identifier : AIDSLINE PRIM10/93200982; Kodama T; Kawahara T; Ferber N; Desrosiers RC; Axthelm MK; Oregon Regional Primate Research Center, Beaverton 97007.; Rhesus macaque Mm155-88 died 27 months after infection with molecularly cloned T-cell tropic SIVmac239. This animal was bilaterally blind at the time of death, and histologically showed severe encephalopathy characterized by an accumulation of multinucleated giant cells within the optic chiasm, cerebral white matter, 1
Restricted replication of SIVmac239 in macrophages is determined by env but is not due to restricted entry.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 19. Unique Identifier : AIDSLINE PRIM10/93200983; Mori K; Ringler D; Desrosiers R; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA 01772-9102.; CD4+ T-lymphocytes and macrophages are the major targets of SIV infection in vivo and in vitro, similar to HIV-1. Molecularly cloned SIVmac239 causes AIDS in rhesus monkeys but it replicates very poorly in primary rhesus monkey alveolar macrophage and blood monocyte cultures. We have documented several cases in which 1
Viral distribution and cellular targets of SIV in intestinal mucosa of rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 2. Unique Identifier : AIDSLINE PRIM10/93200984; Heise C; Vogel P; Lackner A; Dandekar S; Dept. of Internal Medicine, University of California, Davis; 95616.; To examine SIV pathogenesis and the development of intestinal dysfunction, 24 rhesus macaques were inoculated with either a biological isolate of SIV (SIVmac), a pathogenic (SIVmac-239) or non-pathogenic (SIVmac-1A11) clone. Two animals from each group were euthanized at 2, 8, 13 and 23 weeks postinoculation (pi) and 1
Mutational and functional analysis of V3 of SIVmac.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 20. Unique Identifier : AIDSLINE PRIM10/93200985; Kirchhoff F; Desrosiers R; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA 01772-9102.; We have initiated studies to determine whether sequences in SIVmac env that correspond to V3 of HIV-1 are functionally equivalent to V3 of HIV-1. Antibodies raised to V3 of HIV-1 env can neutralize HIV-1 infectivity and the V3 region has been shown to be important for HIV-1 entry into cells. Antibodies raised to the c1
Molecular evolution and epidemiology of SIV during early infection of macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 21. Unique Identifier : AIDSLINE PRIM10/93200986; Hynes NA; Hirsch VM; LID/NIAID/NIH, Rockville, MD.; Many aspects of the early events of SIV-infection of macaques and human AIDS remain poorly defined. In the process of infection, SIV-infected macaques and AIDS patients are apparently exposed to a quasispecies of viral genomes with potentially different pathogenic properties. Whether the host selects a single or multi1
Does sequence diversity of the Nef gene contribute to the disease resistance and susceptibility to SIV infection in naturally infected mangabeys and experimentally infected macaques?: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 22. Unique Identifier : AIDSLINE PRIM10/93200987; Villinger F; Lauro S; Folks TM; Ansari AA; Dept. of Pathology, Emory University School of Medicine, Atlanta,; GA.; Recent studies have demonstrated the in vivo importance of the Nef gene of SIV for the induction of disease in macaques. A large number of sooty mangabeys at the Yerkes Regional Primate Research Center are naturally infected with SIV but, to date, remain asymptomatic. Virus isolated from a mangabey (SIVsmm-9), when in1
In vivo and in vitro effects of SIV mutants deleted in the non-essential genes.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 23. Unique Identifier : AIDSLINE PRIM10/93200988; Gibbs J; Regier D; Daniel M; Desrosiers R; Department of Microbiology, Harvard Medical School, Southborough,; MA.; The so-called non-essential genes of SIV are vif, vpx, vpr and nef. These genes can be deleted with little or no effect on virus growth in vitro under most conditions. However, the roles of these genes remain unclear. Similarly, the importance of the negative regulatory element, or NRE remains to be determined. We hav1
Packaging of SIV RNA into HIV-1 particles; characterization of the SIV encapsidation region.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 24. Unique Identifier : AIDSLINE PRIM10/93200989; Rizvi TA; Panganiban A; McArdle Laboratory for Cancer Research, University of Wisconsin,; Madison 53706.; The putative cis-acting encapsidation sites of SIV and HIV-1 bear no obvious primary sequence similarity. Moreover, while regions of simpler type C retroviruses required for encapsidation have been identified through mutational analysis, the characteristics of the RNA that facilitate incorporation into assembling caps1
The role of NFkB in SIVmac239 replication.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 25. Unique Identifier : AIDSLINE PRIM10/93200990; Bellas R; Hopkins N; Li Y; Center for Cancer Research, Massachusetts Institute of; Technology, Cambridge 02139.; The binding site for nuclear factor kB (NFkB) is highly conserved among isolates of the human and simian immunodeficiency viruses (HIV and SIV, respectively). We have investigated the in vitro growth properties of SIVmac239 possessing 0, 1 (wild-type), or 2 kB sites. These viruses all replicate well in established CD41
Infection of Macaca nemestrina by human immunodeficiency virus type 1: a new primate model for HIV-1 infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 26. Unique Identifier : AIDSLINE PRIM10/93200991; Agy MB; Frumkin L; Corey L; Florey M; Thompson J; Schmidt A; Panther L; Koehler J; Morton WR; Katze MG; Regional Primate Research Center, U. of Washington, Seattle.; We have previously reported HIV-1 in vitro replication in PBMC from M. nemestrina but not in cells from M. fascicularis or M. mulatta. Furthermore, we reported on the infection of 2 M. nemestrina describing virus recovery, detection of HIV-1 specific DNA in PBMC, & antibody response of the infected animals during 1
Lymphomagenesis in SIV immunosuppressed cynomolgus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 27. Unique Identifier : AIDSLINE PRIM10/93200992; Biberfeld P; Feichtinger H; Li SL; Kaaya EE; Ernberg I; Voevodin AF; Putkonen P; Biberfeld G; Immunopathology Lab., Karolinska Institute, Stockholm, Sweden.; OBJECTIVE: To characterize lymphomagenesis observed in malignant lymphomas (ML) of SIV immunosuppressed cynomolgus monkeys. MATERIAL AND METHODS: SIVsm infected cynomolgus monkeys with malignant lymphomas (ML) (15/40) were studied by immunohistopathology, anticomplement immunofluorescence (ACIF), Western blot (WB), po
Analysis of simian T-cell leukemia virus type-1-associated malignancies in a captive baboon colony.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 28. Unique Identifier : AIDSLINE PRIM10/93200993; Mone J; Hubbard G; Leland M; Allan JS; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas.; Simian T-cell leukemia virus type 1 (STLV-I) is a type C retrovirus very similar to human T-cell leukemia virus type 1, the etiologic agent of adult T-cell leukemia/lymphoma in humans. Most species of old-world primates harbor STLV-I, and this virus has been associated with malignancies in several primate species incl
Maternal-fetal transmission of SIV in 2 species of macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 29. Unique Identifier : AIDSLINE PRIM10/93200994; Davison-Fairburn B; Baskin G; Murphey-Corb M; Tulane Primate Center, Covington, LA 70433.; We have previously shown that inoculation of 10 pregnant rhesus monkeys with SIV/DeltaB670 induced 2 abortions coincident with rapid disease progression in the female. The remaining neonates were delivered 10 days prior to term by Caesarean section and found to be uninfected. (Davison-Fairburn et. al. J.Med. Primatol;
In vivo passage of HIV-2 EHO in Macaca nemestrina results in persistent infection, disease progression, and altered cellular tropism.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 3. Unique Identifier : AIDSLINE PRIM10/93200995; McClure J; Misher L; Schmidt A; Steele J; Morton W; Hu SL; Bristol-Myers Squibb, Seattle, WA 98121.; HIV-2EHO is representative of a subgroup of HIV-2 which demonstrates enhanced cytopathogenicity, increased replicative rate, and extended host range in vitro as compared to HIV-2ROD. HIV-2EHO can be further distinguished by the molecular weight of its envelope precursor and external glycoprotein which are 20 kDa small
Animal model for pediatric AIDS: pathology in infant rhesus monkeys infected with SIVSMM through maternal transmission.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 30. Unique Identifier : AIDSLINE PRIM10/93200996; Klumpp SA; Anderson DC; Novembre FJ; McClure HM; Yerkes Regional Primate Research Center, Emory University,; Atlanta, Georgia 30322.; Cases of pediatric acquired immunodeficiency syndrome (AIDS) are increasing due to the rising incidence of HIV infection in women of childbearing age. The literature suggests that disease progression and manifestations of pediatric AIDS are different from adult AIDS. Twenty-five percent of infant rhesus monkeys born t
Vertical transmission of SIVmac239 in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 31. Unique Identifier : AIDSLINE PRIM10/93200997; Mossman SP; O'Neil SP; Hoover EA; Maul DH; Department of Pathology, Colorado State University, Fort Collins; 80523.; We used SIV infection of pregnant macaques to study the targeting of SIV to tissues involved in the transplacental, peri- and post-natal transmission of virus to offspring. Six pregnant rhesus macaques were infected intravenously with 10(4) TCID50 SIVmac239, three at mid-second and three at mid-third trimester. The in
Prenatal inoculation of macaque fetuses with SIV and HIV.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 32. Unique Identifier : AIDSLINE PRIM10/93200998; Ochs HD; Morton WR; Kuller LD; Zhu Q; Tsai CC; Kimpo C; Schmidt A; Agy MB; Benveniste RE; Department of Pediatrics, University of Washington, Seattle; 98195.; To determine the effect of simian (SIV) and human (HIV) immunodeficiency virus on the development of the fetus, we have inoculated pregnant M. nemestrina and their fetuses at various gestational ages with SIVMne or with HIV-1LAI. Intra-amniotic inoculation with SIVMne was attempted in four animals between 91-116 days
Prenatal SIV infection in the rhesus macaque (Macaca mulatta).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 33. Unique Identifier : AIDSLINE PRIM10/93200999; Tarantal AF; Marthas ML; McChesney MB; Miller CJ; Lerche NW; Pedersen NC; Hendrickx AG; California Regional Primate Research Center, University of; California, Davis 95616-8542.; Current information suggests that HIV infection in human infants can occur during all stages of pregnancy. Based on these data and the varied outcome observed for infants infected with HIV, it has been hypothesized that the timing of infection prenatally may be related to the ultimate disease course postnatally. In an
Simian immunodeficiency virus (SIV) infection of infant rhesus macaques as a model to test antiretroviral drug prophylaxis and therapy: oral AZT prevents SIV infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 34. Unique Identifier : AIDSLINE PRIM10/93201000; Van Rompay KK; Marthas ML; Ramos RA; Mandell CP; McGowan EK; Joye SM; Pedersen NC; California Regional Primate Research Center, University of; California, Davis 95616.; The prophylactic and therapeutic properties of 3 -azido-3 -deoxythymidine ( AZT ) against simian immunodeficiency virus (SIV) infection were tested in 4 three-month-old rhesus macaques. The infant monkeys were inoculated intravenously with a low dose (1
Inactivated SIV in biodegradable microspheres protects rhesus macaques from vaginal challenge.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 35. Unique Identifier : AIDSLINE PRIM10/93201001; Marx PA; Gettie A; Eldridge JH; Staas JK; Gilley RM; Mulligan MJ; Mestecky J; Compans RW; Div. of Virology and Immunology, New Mexico State University,; Holloman AFB 88330.; To test biodegradable copolymer poly (DL-lactide-co-glycolide) (DL-PLG) as a vaccine formulation for mucosal immunity and protection in the simian immunodeficiency virus (SIV) model, 7 macaques received 3 IM injections which consisted of 100 micrograms of formalin-inactivated SIVmac251 (Davis stock) in DL-PLG microsph
Immunization with recombinant SIVmne gp160 vaccines partially protects macaques against uncloned homologous virus challenge.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 36. Unique Identifier : AIDSLINE PRIM10/93201002; Hu SL; Stallard V; Misher L; Langlois AJ; Kuller LR; Morton WR; Benveniste RE; Bristol-Myers Squibb, Pharmaceutical Research Institute, Seattle,; WA.; We previously reported that immunization with recombinant SIVmne gp160 vaccines protected macaques against a low-dose challenge by a cloned homologous virus. We have extended these studies to investigate whether the same immunized animals would also be protected against uncloned homologous virus challenge. Four Macaca
HIV-2 and SIV vaccine efficacy studies using monkey cell grown challenge viruses.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 37. Unique Identifier : AIDSLINE PRIM10/93201003; Putkonen P; Thorstensson R; Biberfeld G; Department of Immunology, National Bacteriological Laboratory,; Stockholm, Sweden.; HIV-2 VACCINE STUDY: Four cynomolgus monkeys were first immunized intramuscularly five times with an inactivated HIV-2 preparation (total dose 0.7 mg) in iscoms over a period of 16 months and then boosted at 18 and 20 months with HIV-2 V3 synthetic peptide of HIV-2 gp 125 coupled to iscoms. The four vaccinated animals
Passive immunization with antibodies to conserved envelope peptides protects rhesus macaques from SIV infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 38. Unique Identifier : AIDSLINE PRIM10/93201004; Lewis MG; Shafferman A; McCutchan FE; Benveniste RE; Montifiori DC; Lai CY; Bellah SW; McKinnon KM; Yalley-Ogunro J; Burke DS; et al; Henry M. Jackson Foundation, Rockville MD 20850.; We have previously reported that naive macaques immunized with four conserved peptides (two from the gp120 and two from the gp41) fused to beta-galactosidase (beta gal) and formulated in complete Freund s adjuvant have an altered disease course following infection with SIVmne/E11s. The objective of this study was to e
Horizontal transmission of SIV in juvenile male rhesus immunized with inactivated, whole-virus vaccine.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 39. Unique Identifier : AIDSLINE PRIM10/93201005; Jennings MB; Lowenstine LL; Rosenthal A; Yee JL; Cotterman R; Antipa L; Hendrickx A; Gardner MB; Department of Pathology, School of Medicine, University of; California, Davis.; Four healthy juvenile male rhesus (Macaca mulatta) were inoculated IV with SIVmac and put in a group cage with 4 control animals and 4 animals immunized with inactivated, whole-virus vaccine previously shown to protect against cell-free, IV challenge with SIVmac. All four inoculates were antigenemic and PBMC culture p
Dynamics of the immune system response in cerebrospinal fluid and blood of SIVmac-infected rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 4. Unique Identifier : AIDSLINE PRIM10/93201006; Sopper S; Hemm S; Meixensberger J; Coulibaly C; Stahl-Hennig C; Hunsmann G; ter Meulen V; Dorries R; Institut fur Virologie und Immunbiologie, Universitat; Wurzburg, Germany.; Infection by the human immunodeficiency virus (HIV) regularly afflicts the central nervous system (CNS). To date, little is known about possible relationships between CNS-localized immune responses and HIV-associated encephalopathies. For obvious reasons studies of this question are severely hampered in patients. Thus
A type D SRV-2 virus-specific CD8 T cell that regulates virus-induced T cell proliferation in Celebes macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 40. Unique Identifier : AIDSLINE PRIM10/93201007; Malley A; Pangares N; Mayo SK; Zeleny-Pooley M; Torres JV; Benjamini E; Axthelm MK; Oregon Regional Primate Research Center, Beaverton 97006.; Previous studies in mice and rhesus macaques demonstrated that SRV-1 and SRV-2 viruses induce significant virus-specific T cell proliferation. In addition, these studies defined SRV-2 envelope peptide 96-102 (unique to SRV-2), and envelope peptides 233-249 and 127-152 (shared by SRV-1 and SRV-2) as T cell epitopes tha
Role of SIV-specific cellular and humoral immunity in protection against SIV infection in macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 41. Unique Identifier : AIDSLINE PRIM10/93201008; Mills KH; Jones WC; Kitchin PA; Page M; Chan WL; Taffs F; Bruck C; Thiriart C; DeWilde M; Rud E; et al; NIBSC, Potters Bar, Herts, U.K.; Although protection against SIV infection has been demonstrated by immunization with inactivated SIV, we and others have shown that the protective immune response may be, in part, generated by human T cell components of the crude virus vaccines. The reported success with recombinant SIV vaccines is limited to a study
Beta 2-microglobulin and HLA class II DR co-purify with HIV and SIV, and antisera to these proteins neutralize virus infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 42. Unique Identifier : AIDSLINE PRIM10/93201009; Benveniste R; Henderson L; Sowder R 2d; Bess J Jr; Mann D; Arthur L; National Cancer Institute, Frederick, MD 21702.; Previous studies with subunit vaccines have shown that immunization with viral envelope antigens alone are sufficient to elicit protective immunity against SIV or HIV. The finding that macaques immunized with uninfected human cells were protected against challenge from SIV grown in human cells (Stott, Nature, 1991) ra
SIV and STLV-1 seroprevalence in east African nonhuman primate species.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 43. Unique Identifier : AIDSLINE PRIM10/93201010; Aman RA; Isahakia M; Villinger F; Mayne A; Jehuda-Cohen T; Powell J; Vuchetich M; Suleman MA; Chege A; Chirchir I; et al; Institute for Primate Research, Karen, Nairobi, Kenya.; Blood samples from a total of 176 nonhuman primates of various species were screened for the presence of antibodies that react by ELISA and Western blots (W.B.) against SIVsmm, HIV-2, and STLV-1. In addition, PBMC from each sample was cultured in vitro with pokeweed mitogen, and the supernatant fluid was assayed for a
Identification of the V1 region of the SIV surface glycoprotein as a neutralization determinant.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 44. Unique Identifier : AIDSLINE PRIM10/93201011; Anderson MG; Joag SV; McEntee MF; Narayan O; Clements JE; Johns Hopkins University School of Medicine, Baltimore, Md 21205.; Lentiviruses of horses, sheep and humans have been shown to undergo antigenic variation within the host. To examine whether this occurs in macaques infected with molecularly cloned virus, a monkey (2D) was infected with SIVmac239 and the presence of neutralizing antibody was identified. Viruses were isolated at variou
Analysis of the frequencies of TH0, TH1, and TH2 subsets of cloned lymphoid cells in mangabeys and macaques, based on cytokine profiles.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 45. Unique Identifier : AIDSLINE PRIM10/93201012; Ansari AA; Mayne A; Hunt D; Villinger F; Dept. of Pathology, Emory University School of Medicine, Atlanta,; GA.; Recent evidence suggests that human lymphoid T cells can be distinguished into subsets based on the cytokine profile of each subset, similar to that described in the murine system. TH0 cells are classified as those TH cells that secrete IL-2 , IFN-gamma, IL-4, IL-6, and
Staphylococcal enterotoxins (superantigens) increase viral expression in SIV-infected lymphocytes from Macaca nemestrina.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 46. Unique Identifier : AIDSLINE PRIM10/93201013; Axberg I; Polacino-Firpo P; Clark EA; Regional Primate Research Center, University of Washington,; Seattle 98195.; Staphylococcal enterotoxins, also called superantigens (SAg), are proteins secreted from Staphylococcus aureus (S. aureus) that can cause food poisoning in man and experimental animals. We have found that SAg stimulate macaque peripheral blood lymphocytes (PBL) to proliferate, and the TCR V beta expression changes dep
The induction of heat shock proteins in cells infected with SIV251.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 47. Unique Identifier : AIDSLINE PRIM10/93201014; Bartz SR; Welch WJ; Malkovsky M; Department of Medical Microbiology and Immunology, University of; Wisconsin, Madison 53706.; It has been observed that some viruses (both DNA and RNA) can activate the synthesis of heat shock proteins (HSP s). By Western blotting we show that the infection of MOLT4 clone 8 cells by SIV251 results in an increase in HSP70 and HSP58. In addition, the increase in HSP70 and HSP58 appears to be differentially regul
Pathologic effects of lymphotropic and macrophage-tropic simian immunodeficiency virus (SIVMAC) correlate with decreases in macrophage precursors in progenitor-enriched bone marrow populations obtained by counterflow centrifugal elutriation (CCE).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 48. Unique Identifier : AIDSLINE PRIM10/93201015; Beltz LA; Zink MC; Adams RJ; Narayan O; Donnenberg AD; University of Pittsburgh School of Medicine, PA 15213.; Pathological findings were compared between groups of rhesus macaques infected with SIV delta (n = 2), SIV251 (n = 3), molecularly cloned lymphotropic SIV239 (n = 3), or SIV239 passaged in vivo (macrophage-tropic, SIV239M phi, n = 7). The frequency of functional macrophage (M phi) progenitors (M phi Pro) in bone marro
Early stages of SIV-induced lymph node pathogenesis in the rhesus macaque.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 49. Unique Identifier : AIDSLINE PRIM10/93201016; Chakrabarti L; Isola P; Cumont MC; Claessens-Maire MA; Hurtrel M; Montagnier L; Hurtrel B; Unite d'Oncologie Virale, Institut Pasteur, Paris, France.; The occurrence of marked symptoms prior to seroconversion are thought to have predictive value for the risk of development of AIDS. Characterization of the early stages of HIV-induced pathogenesis is therefore needed to understand the parameters determining the evolution towards AIDS. Experimental inoculation of SIVma
Early intrathecal events in rhesus macaques infected with pathogenic (SIVmac-239) or nonpathogenic (SIVmac-1A11) clones of SIVmac or with biologic SIVmac.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 5. Unique Identifier : AIDSLINE PRIM10/93201017; Smith MO; Heyes MP; Lackner AA; California Regional Primate Research Center, UC Davis.; We examined changes in cerebrospinal fluid (CSF) in SIV-infected rhesus macaques during the early stages of infection and correlated this data with disease findings at necropsy. Twenty four juvenile male rhesus macaques were divided into groups of 8 and inoculated intravenously with equal doses of biologic SIVmac (der
Detection of simian T-cell leukemia virus type 1 (STLV-1) in lymphomatous tissues from SIV-positive macaques that died of simian AIDS.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 50. Unique Identifier : AIDSLINE PRIM10/93201018; Chikobava M; Shatzel H; Lowenstine L; Yakovleva L; Lapin B; Russian Academy of Medical Sciences, Sochi-Adler Russian; Federation.; Formalin-fixed, paraffin-embedded lymphoid tissues from four stump-tailed macaques (Macaca arctoides), that died of simian AIDS accompanied by lymphoma in 1969-70 at the California Primate Research Center and that were known to be seropositive for SIV, were examined retrospectively for STLV-1 using PCR. Two primer pai
Tumor necrosis factor in SAIDS.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 51. Unique Identifier : AIDSLINE PRIM10/93201019; Didier ES; Soike KF; Tulane University Regional Primate Research Center, Covington, LA; 70433.; Tumor necrosis factors (TNF-alpha and -beta) play important roles during viral, bacterial and parasitic infections. Beneficial effects of TNF include regulating immune responses for controlling pathogen growth, however, TNF also contributes to the cachexia and septic shock sometimes associated with these infections. T
Biological comparison of the LTR sequences from the acutely pathogenic SIVSMMPBj14 and a closely related non acute strain.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 52. Unique Identifier : AIDSLINE PRIM10/93201020; Dollard SC; Fultz PN; Dewhurst S; Dept. of Microbiology and Immunology, University of Rochester; Medical Center, NY 14642.; Molecularly cloned SIVs capable of inducing acute fatal disease in pig-tailed macaques have been derived previously from a biological clone (bcl-3) of the PBj14 isolate of SIV from sooty mangabey monkeys (SIVSMMPBj14). A closely related biological clone (bcl-1), also derived from the PBj14 isolate, fails to induce acu
Differing infectivity of SIVsm and HIV-1 for human and rhesus cell-lines expressing human/rhesus chimeric CD4 molecules.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 53A. Unique Identifier : AIDSLINE PRIM10/93201021; Fomsgaard A; Johnson PR; Hirsch VM; Dept. of Clin. Microbiol., University Hospital of Copenhagen,; Denmark.; Differences in the pathogenicity, cellular tropism and cytopathic effects of various HIV and SIV may depend on host-specific factors in addition to viral determinants. CD4 is the cellular receptor for HIV and SIV and variation in CD4 structures involved in virus binding (V1-domain), entry, and syncytia formation (J1-d
Induced simian immunodeficiency virus (SIVmne) infection in cynomolgus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 53. Unique Identifier : AIDSLINE PRIM10/93201022; Follis KE; Tsai CC; Grant R; Wu HN; Benveniste RE; Regional Primate Research Center, University of Washington,; Seattle 98195.; Seventeen naive cynomolgus macaques were used for this study. An uncloned SIVmne viral stock that had been propagated in a human T cell line was used for intravenous inoculation (viral challenge). First, in order to determine the MID50 (monkey infectious dose 50%) for SIVmne, ten macaques were challenged with 10 fold
Evidence for increased cytokine production in early stages of SIV infection of macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 54. Unique Identifier : AIDSLINE PRIM10/93201023; Fox LM; Poli G; Hirsch VM; Fauci AS; Laboratory of Immunoregulation, NIAID, NIH, Bethesda, MD 20892.; Pro-inflammatory and immunoregulatory cytokines are capable of stimulating both HIV and SIV expression in vitro. Elevated cytokine levels in the plasma or secreted in vitro from peripheral blood mononuclear cells (PBMC) have been found in individuals infected with HIV, and from alveolar macrophages of macaques infecte
DNA sequence analysis of the env region of HIV-2 isolates from a macaques transmission study.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 55. Unique Identifier : AIDSLINE PRIM10/93201024; Grant R; Misher L; Schmidt A; Morton WR; McClure J; Hu SL; University of Washington Primate Research Center, Seattle.; We previously reported that two prototype HIV-2 isolates, ROD and EHO, have distinct in vitro and in vivo properties. The ROD isolate is minimally cytopathic in macaque peripheral blood mononuclear cells (PBMC) and has a surface glycoprotein of 130 kD as determined by gel electrophoresis. The EHO isolate is highly cyt
SIV-associated diarrhea and enteropathy in the African green monkey.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 56. Unique Identifier : AIDSLINE PRIM10/93201025; Guhad FA; Farah IO; Chirchir IO; Machai PN; Institute of Primate Research, Karen, Nairobi, Kenya.; Clinical and necropsy records from 22 African Green Monkeys that were naturally infected with SIV virus and had died of diarrheal diseases were reviewed. The relationship between serostatus and diarrhea as well as enteropathy was evaluated. The seropositive monkeys (n = 10) had averages of 6 bouts and 12.6 days of dia
The benign course of HIV-1 in chimpanzees may be reflected by the infection resistant nature of cells with antigen presenting function.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 57. Unique Identifier : AIDSLINE PRIM10/93201026; Heeney J; Miedema F; Kootstra N; Meyaard L; Koornstra W; Dubbes R; Tersmette M; Schuitemaker H; Laboratory of Viral Pathogenesis, ITRI-TNO, Rijswijk, The; Netherlands.; We propose that one mechanism of lymphocyte dysfunction and death in the development of AIDS may be due to altered lymphocyte activation signals caused in part by defects of infected antigen presenting cells (APC). Unlike human peripheral blood lymphocytes (PBLs) we found that in all but one chimpanzee we were unable
The natural history of early simian immunodeficiency virus infection of macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 58. Unique Identifier : AIDSLINE PRIM10/93201027; Hynes NA; Goldstein S; London WT; Elkins WR; Hirsch VM; LID/NIAID/NIH, Rockville, MD.; The natural history of simian immunodeficiency virus (SIV) infection of macaques remains poorly understood. This is particularly true of the early stages of infection when critical virus-host interactions may determine the ultimate disease course of infected animals. Early events may play a similar role in HIV-1 infec
Antigenic variation of molecularly cloned SIVmac239 during persistent infection in a rhesus macaque.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 59. Unique Identifier : AIDSLINE PRIM10/93201028; Joag SV; Anderson MG; Clements JE; McEntee MF; Adams RJ; Narayan O; Division of Comparative Medicine, Johns Hopkins University School; of Medicine, Baltimore, MD 21205.; 8 rhesus macaques inoculated with SIVmac239 developed viremia and virus-binding antibodies, but only one (macaque 2D) developed neutralizing antibodies to the virus. Macaque 2D plasma also neutralized SIVmac251, although at a lower titer. Viremia persisted in macaque 2D even in the presence of neutralizing antibodies.
VCAM-1 is induced and mediates monocyte adhesion to endothelium in SIV encephalitis.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 6. Unique Identifier : AIDSLINE PRIM10/93201029; Sasseville V; Newman W; Lackner A; Smith M; Lausen N; Hesterberg P; Beall D; Ringler D; Division of Pathology, Harvard Medical School, Southborough, MA.; SIV/HIV encephalitis is characterized by the presence of parenchymal and perivascular macrophages and multinucleated giant cells in the brain. Like other inflammatory responses in the brain, these cells, as monocytes, traverse from the vascular lumen to the Virchow-Robin space. Requisite for this process is the succes
Infection of Macaca nemestrina with HIV-2/EHO by vaginal inoculation with and without Lactobacillus acidophilus.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 60. Unique Identifier : AIDSLINE PRIM10/93201030; Klebanoff S; Morton W; Hillier S; McClure J; Thompson J; Schmidt A; University of Washington, Seattle 98195.; Peroxidases, combined with H2O2 and halide, form a powerful antimicrobial system that is effective against a variety of microorganisms, including viruses. Lactic acid bacteria, including lactobacilli, generate H2O2 that can be toxic to bacteria, fungus, and viruses, particularly when combined with peroxidase and halid
Successful intrarectal inoculation of two macaque species with SIVMne CL E11S.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 61. Unique Identifier : AIDSLINE PRIM10/93201031; Kuller L; Benveniste RE; Clark EA; Katze MG; Morton WR; Regional Primate Research Center, University of Washington,; Seattle 98195.; In a previous study of six M. nemestrina inoculated intrarectally with SIVMne CL E11S, a single-cell clone of SIVMne, virus was isolated at various time points from the animals inoculated with 1 x 10(4) and 1 x 10(3) tissue culture infectious dose (TCID) and one animal died of AIDS at 19 weeks post-inoculation. In a m
Specificity, sensitivity, and reproducibility for the detection of SIV-specific antibodies in the serum and supernatant fluid of PWM-stimulated PBMC from mangabeys and macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 62. Unique Identifier : AIDSLINE PRIM10/93201032; Mayne A; Jehuda-Cohen T; Villinger F; Ansari AA; Dept. of Pathology, Emory Univ. School of Medicine, Atlanta, GA.; Most laboratories either utilize the commercially available HIV-2 kit or laboratory-prepared SIV preparations for the detection of SIV-specific antibodies by ELISA and then perform Western blot or RIPA on ELISA-positive samples for confirmatory purposes. The relative specificity, sensitivity, and reproducibility of th
Isolation of a new foamy virus from an orangutan with encephalopathy.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 63. Unique Identifier : AIDSLINE PRIM10/93201033; McClure MO; Schulz TF; Simpson G; Christie I; Cunningham A; Kirkwood J; Ali M; Weiss RA; Department of Genitourinary Medicine, St Mary's Hospital Medical; School, London.; Although Foamy viruses (Spumaretroviruses) have been isolated from a variety of species, including on rare occasions from humans, their association with any disease is still controversial. We report here the isolation of a new foamy virus form, and the prevalence of neutralising antibodies to this virus in, captive Or
Serial in vivo passage of SIV from infected macaques with end stage viremia results in increase in strain virulence.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 64. Unique Identifier : AIDSLINE PRIM10/93201034; Niphuis H; Holterman L; Dubbes RH; Koornstra WH; Laman JD; Baskin GB; Murphy-Corb M; Heeney JL; Dept. of Chronic and Infectious Diseases, ITRI-TNO, Rijswijk, The; Netherlands.; To determine if repeated in vivo passage of SIVB670 resulted in increased virulence, peripheral blood mononuclear cells (PBMC) of endstage SIV infected macaques were passed intravenously into naive animals. Ten age-matched juvenile rhesus macaques were infected with an equivalent dose of SIVB670. The first macaque to
Structure of nef genes of long term healthy survivors of HIV-1 infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 65. Unique Identifier : AIDSLINE PRIM10/93201035; Premkumar D; Salkowitz J; Hirsch M; Kestler HW; Department of Molecular Biology, Cleveland Clinic Foundation.; The nef gene of molecular clone SIVmac239 has been shown to be required for viral pathogenesis and for maintaining high virus loads in infected rhesus monkeys. The deletion of 182 base pairs in the nef gene of SIVmac239 resulted in virus that was unable to sustain high loads and eliminated its pathogenic potential (Ke
Early events in simian immunodeficiency virus-infected macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 66. Unique Identifier : AIDSLINE PRIM10/93201036; Ramos RA; Lohman BL; Van Rompay KK; Miller CL; Marthas ML; Department of Medicine, School of Veterinary Medicine, University; of California, Davis.; High cell-associated virus load and high plasma virus titers have been detected between 2 and 23 weeks post-inoculation (p.i.) in macaques infected with pathogenic SIV (uncloned SIVmac and SIVmac239), but not nonpathogenic SIVmac1A11 (R.A. Ramos, et al., manuscript in preparation). This suggests that critical events w
Association of arthritis in SIV infected rhesus macaques with Shigella flexneri breakdown products in the intestine, mesenteric lymph node and synovium.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 67. Unique Identifier : AIDSLINE PRIM10/93201037; Roberts ED; Smith V; Dept. of Pathol., Tulane Regional Primate Research Center,; Covington, Louisiana 70433.; Arthritis is increasingly being recognized in HIV patients who test negative for rheumatoid factor, negative for HLA-B27 and negative for psoriatic arthritis. A reactive joint disease has been recently documented in SIV/Delta-infected rhesus macaques. A primary-viral arthritis was demonstrated in only 11% of the macaq
Immunological deterioration and proviral levels in LN of SIV-infected macaques predicts progression to AIDS.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 68. Unique Identifier : AIDSLINE PRIM10/93201038; Rosenberg YJ; Zack PM; Greenhouse JJ; Leon EC; Papermaster SF; White BD; Eddy GA; Burke DS; Lewis MG; Henry M. Jackson Foundation Research Laboratory, Rockville, MD.; Lymphocytes in the blood constitute only 1-2% of the total pool. Although the decline in blood CD4+ cells during HIV and SIV infections is well characterized and has predictive value, how these changes reflect those in lymphoid organs which contain the bulk of lymphocytes is not well understood. Studies in 15 monkeys
The molecular basis of simian immunodeficiency virus (SIV) NEF function.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 69. Unique Identifier : AIDSLINE PRIM10/93201039; Salkowitz J; Premkumar D; Kestler HW; Department of Molecular Biology, Cleveland Clinic Foundation.; The nef gene of the pathogenic molecular clone SIVmac239 was shown to be required for viral pathogenicity and for maintenance of high virus loads in rhesus monkeys (Kestler et al., Cell 65: 651-662). Deletion of 182 base pairs in the nef gene produced a virus that was unable to sustain high loads observed in rhesus mo
Early events and cellular targets in bone marrow of SIV-infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 7. Unique Identifier : AIDSLINE PRIM10/93201040; Mandell CP; Jain NC; Miller CJ; Marthas M; Dandekar S; Department of Clinical Pathology, California Primate Research; Center, Davis.; Our previous studies detected SIV-infected mononuclear cells in bone marrow (BM) as early as 2 weeks post-inoculation. Prominent histologic abnormalities included myeloid hyperplasia and megakaryocytic hyperplasia. The purpose of this study was to identify the in vivo cellular targets in BM in early SIV infection and
Expression of cell adhesion molecules in the jejunum of SIV infected rhesus macaques (Macaca mulatta).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 70. Unique Identifier : AIDSLINE PRIM10/93201041; Stone J; Elices M; Canfield D; Dandekar S; Dept. of Internal Medicine, University of California, Davis; 95616.; SIV-infected rhesus macaques experiencing malabsorption had lymphocytic and histiocytic inflammatory infiltrates accompanied by high levels of SIV RNA in the jejunum. Leukocyte adhesion molecules participate in many leukocyte functions needed for immune and inflammatory responses, by specific receptor-ligand interacti
The role of interferon in the development of AIDS.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 70A. Unique Identifier : AIDSLINE PRIM10/93201042; Tovey MG; Lebon P; Meyer F; Hurtrel B; Gresser I; Venet A; Girard M; Aubertin AM; IRSC-CNRS, Villejuif, Hopital Saint-Vincent de Paul, Strasbourg,; France.; Considerable evidence suggests that co-factors including cytokines play a determining role in the development of the acquired immunodeficiency syndrome (AIDS) following infection by the human immunodeficiency virus (HIV). The presence of acid-labile interferon (IFN) alpha in the sera of AIDS patients has been shown to
Neuro-ophthalmic manifestations of simian immunodeficiency virus infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 71. Unique Identifier : AIDSLINE PRIM10/93201043; Vogel P; Lackner AA; Lowenstine LJ; Miller CJ; California Regional Primate Research Center, UC Davis.; Ophthalmic lesions are seen in up to 94% of AIDS patients and often are the result of opportunistic infections or neoplasms. The existence of a primary HIV-induced retinopathy has been difficult to demonstrate in humans because of frequently superimposed CMV-induced lesions in terminal AIDS. HIV has been isolated from
Rearing history and survival time in SIV-infected juvenile rhesus monkeys (Macaca mulatta).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 72. Unique Identifier : AIDSLINE PRIM10/93201044; Clarke MR; Harrison RM; Martin LN; Tulane Regional Primate Research Center, Covington LA 70433.; Data from two samples of SIV-infected animals were analyzed to compare developmental, social, and environmental histories with survival time following inoculation. The first data set included animals that were infected for different research protocols (e.g., progression of disease, controls for drug studies), and incl
Cyclic patterns of cell free SIV production and spread in peripheral blood of rhesus macaque monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 73. Unique Identifier : AIDSLINE PRIM10/93201045; Emau P; MacKenzie D; Malkovsky M; Pauza CD; Department of Pathology & Laboratory Medicine, University of; Wisconsin, Madison 53706.; We examined the temporal changes in cell free virus titres to determine the humoral mechanisms that control virus dissemination in rhesus monkeys. Plasma virus titres were compared to accumulated levels of total SIV DNA, circular viral DNA, SIV-specific antibody and CD4+ T cells. Three monkeys were infected by either
Prevalence of antibodies to HTLV-1/STLV-1 in a large colony of captive chimpanzees.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 74. Unique Identifier : AIDSLINE PRIM10/93201046; Harrison RE; Murthy KK; Melendez GI; Tuck PA; Eichberg JW; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, TX 78228.; Serum samples from a colony of 198 chimpanzees (Pan troglodytes) were screened for the presence of antibody to HTLV-1/STLV-1 by enzyme immunoassay. All positive serum was analyzed for confirmation by Western blot; 20 of the 198 samples were confirmed to be positive. No evidence of vertical transmission of infection wa
Establishment of quantitative cytokine assays and detection of mRNA coding for the cytokines in lymphoid cells from rhesus macaques and sooty mangabeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 75. Unique Identifier : AIDSLINE PRIM10/93201047; Hunt D; Vuchetich M; Villinger F; Findley H; Mayne A; Folks T; Ansari AA; Dept. of Pathology, Emory Univ. School of Medicine, Atlanta, GA.; Certain select cytokines such as TNF have been shown to markedly influence the replication of lentivirus in vitro, and marked increases of cytokines such as IL-6 have been shown to occur in the sera of lentivirus-infected humans and nonhuman primates. It is hypothesized that qualitative and/or quantitative changes in
CD8 mediated suppression generated in macaques following vaccination with SIV GP160 does not affect viral load after challenge.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 76. Unique Identifier : AIDSLINE PRIM10/93201048; Lohman B; McChesney M; Ahmed S; Giavedoni L; Yilma T; Marthas M; California Regional Primate Research Center, University of; California, Davis 95616.; Immunization with SIVgp160 does not protect against primary infection with SIVmac (T. Yilma, et al., abstract submitted), but we have found that some immunized groups have decreased viral load after challenge. CD8 mediated suppression has been proposed as a mechanism of controlling HIV and SIV infection. In this study
SIVagm in lymphoid tissues of experimentally infected African green monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 77. Unique Identifier : AIDSLINE PRIM10/93201049; Miller CL; Ramos RA; Vogel AP; Isahakia M; Marthas ML; Marx PA; California Regional Primate Research Center, University of; California, Davis.; The simian immunodeficiency virus (SIVagm) isolated from African green monkeys (AGMs) is a lentivirus distantly related to SIVmac. This virus does not cause simian acquired immune deficiency (SAIDS) in its natural host. Other investigators have demonstrated that virus can be recovered from peripheral blood mononuclear
In vivo reactivation of SRV-2/W by experimentally-induced immunosuppression.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 78. Unique Identifier : AIDSLINE PRIM10/93201050; Moazed TC; Maggio-Price L; Shiota F; Kennedy B; Thouless ME; Department of Pathobiology, University of Washington, Seattle; 98195.; SRV-2/W (Simian Retrovirus serotype 2 Washington) is a widespread retrovirus of pig-tailed macaques (M. nemestrina) at the University of Washington Regional Primate Research Center (UWRPRC). After infection and seroconversion the virus is assumed to establish latency with affected animals demonstrating no apparent cli
Primary human brain capillary endothelial cells are permissive for HIV infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 79. Unique Identifier : AIDSLINE PRIM10/93201051; Moses AV; Bloom FE; Pauza CD; Nelson JA; Department of Immunology, Scripps Research Institute, La Jolla,; CA 92037.; The AIDS dementia complex is a sequelae commonly observed in HIV-infected individuals, but the pathogenic mechanisms involved in this neurological disorder are still obscure. The principal cellular targets identified for HIV in the CNS are cells of the macrophage lineage and brain
The absence of T-cell priming for programmed cell death and GP120 induced anergy correlates with natural resistance of chimpanzees to immunosuppressive disease development.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 8. Unique Identifier : AIDSLINE PRIM10/93201052; Heeney J; Gougeon ML; Di Rienzo AM; Montagnier L; Laboratory of Viral Pathogenesis, ITRI-TNO, Rijswijk, The; Netherlands.; A cohort of chimpanzees infected experimentally or naturally with HIV-1 or related lentiviruses has been studied together with uninfected controls for evidence of disease progression. The aim of this study group is to elucidate possible mechanisms of natural disease resistance in this species to the development of AID
Biosafety guidelines for working with non-human primates in retrovirus research.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 80A. Unique Identifier : AIDSLINE PRIM10/93201053; Richmond JY; Brown B; Khabbaz R; Kaplan J; Centers for Disease Control, Atlanta, GA 30333.; Guidelines for work with infectious pathogens in laboratory animals are detailed in the CDC/NIH publication, Biosafety in Microbiological and Biomedical Laboratories. These guidelines describe suitable precautions for working with pathogens at various levels of containment (i.e., Animal Biosafety Levels 1, 2, 3, and 4
Fetal and postnatal immune responses to S.I.V. following in utero infection of rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 80. Unique Identifier : AIDSLINE PRIM10/93201054; Otsyula M; Tarantal A; Lerche N; Marthas M; Miller C; Pedersen N; Hendrickx A; McChesney M; California Regional Primate Research Center, University of; California-Davis 95616-8542.; Infection of infants with the Human Immunodeficiency Virus results in a fatal immunodeficiency disease in infancy or in later childhood. We have used the animal model of AIDS, Simian Immunodeficiency Virus (SIV) infection of rhesus macaques, to understand disease pathogenesis in pediatric HIV infection. Rhesus monkey
SIV variants evolving in vivo from molecularly cloned virus are fast replicating and highly cytopathic to cells in culture.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 81. Unique Identifier : AIDSLINE PRIM10/93201055; Rudensey L; Overbaugh J; Department of Microbiology, University of Washington, Seattle; 98195.; We have previously demonstrated that the progression to simian AIDS is associated with the evolution of envelope genotypes with mutations primarily localized to the V1 and V4 regions of the SIV env gene. A representative mixture of the variants present at later stages of disease in the animal was maintained when the v
Activation of macaque lymphocyte subsets by SIVsmmPBj14.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 82. Unique Identifier : AIDSLINE PRIM10/93201056; Schwiebert R; Fultz PN; Department of Comparative Medicine, University of Alabama,; Birmingham 35294.; In vitro SIVsmmPBj14 replicates efficiently in both resting and mitogen-stimulated PBMCs and appears to induce proliferation of cells as virus accumulates in the culture supernatants. Infection of pig-tailed macaques (Macaca nemestrina) with SIVsmmPBj14 generally results in acute disease and death in 1 to 2 weeks. Acu
Transfection and transformation with simian AIDS-RF DNA in NIH3T3 cells.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 83. Unique Identifier : AIDSLINE PRIM10/93201057; Tsai CC; Wu HN; Malinak CJ; Grant RF; Regional Primate Research Center, University of Washington,; Seattle 98195.; Certain pigtailed macaques with simian D-retrovirus serotype 2 (SRV-2)-induced simian acquired immunodeficiency syndrome (AIDS) have a high incidence of retroperitoneal fibromatosis (RF) and other neoplasia. The mechanisms of SRV-2 induced AIDS-associated neoplasia have not been determined. We investigated the transfo
The failure of a recombinant SIV vaccine is not solely due to the evolution of escape mutants in vivo.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 84. Unique Identifier : AIDSLINE PRIM10/93201058; Almond N; Jenkins A; Heath AB; Kitchin P; AIDS Collaborating Centre, NIBSC, Potters Bar, UK.; To date there has been only one report describing the successful use of a monotypic recombinant vaccine to protect macaques against experimental infection with SIV (Hu et al, Science, 1992, 255, 456). One reason for this may be the variability of the retroviral genome. Thus, new viral sequence variants emerge upon inf
Characterization of a SRV-2 3' open reading frame present in virus infected tumor cells.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 85. Unique Identifier : AIDSLINE PRIM10/93201059; Grant RF; Malinak CJ; Wu H; Tsai CC; University of Washington Primate Research Center, Medical Lake; 99022.; The polymerase chain reaction (PCR) was used to amplify SRV-2 messenger RNA from several cases of retroperitoneal fibromatosis (RF). mRNA was purified from frozen tissue samples of RF origin dating from 1988 through 1992. Complementary DNA (cDNA) was synthesized using reverse transcriptase and the cDNA was used as tem
Isolation, cloning, and sequencing of simian foamy virus from chimpanzee.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 86. Unique Identifier : AIDSLINE PRIM10/93201060; Herchenroder O; Renne R; Mergia A; Cobb KE; Murthy KK; Luciw PA; Dept. of Medical Pathology, University of California, Davis; 95616.; Foamy viruses, also called syncytium-forming or spumaviruses, are one of seven genera of retroviruses and have been found in various species including primates and man. Apparently they induce a latent infection in their host. Although foamy viruses infect several cell types representing various tissues of numerous spe
Partial characterization of SIVsm chronically infected CEM X 174 cells.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 87. Unique Identifier : AIDSLINE PRIM10/93201061; Hernandez S; Beltran M; Lavergne J; Malaga C; Kessler M; Kraiselburd E; SIVsm chronically infected cultures were obtained after infection of CEM X 174 cells with either SIVsm H3 or SIVsm/E660. These phenotypically CD4-cells, formed syncytia when cocultivated with CD4+ cells, and produced infectious, as well as non-infectious viruses. Single cell clones derived from these cultures were gro
Simian AIDS type D retrovirus serotype 2 and variant viruses: molecular cloning and sequence analyses of the envelope glycoprotein genes.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 88. Unique Identifier : AIDSLINE PRIM10/93201062; Horenstein GR; Neilson K; Kelley RD; Crabtree L; Searles RP; Nipper V; Hallick LM; Axthelm MK; Machida CA; Dept. of Microbiology, Oregon Health Sciences University,; Portland 97201.; Type D simian retrovirus (SRV) infection is the cause of most Simian Acquired Immunodeficiency Syndrome (SAIDS)-associated mortality in biomedical research colonies of Asian macaques. Serotype 2 retroviruses have been associated with severe immunodeficiency and retroperitoneal fibromatosis in Celebes, pigtailed, and l
tat relieves the host dependence of HIV in stimulated primary human macrophages.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 89. Unique Identifier : AIDSLINE PRIM10/93201063; Moses AV; Ibanez CE; Gaynor R; Nelson JA; Ghazal P; Department of Immunology, Scripps Research Institute, La Jolla,; CA 92037.; Macrophages constitute a key cell lineage harboring HIV in AIDS patients. Stimulation of macrophages from asymptomatic individuals via a T cell mediated pathway results in reactivation of HIV replication. We have utilized an in vitro model system to identify transcription elements that regulate latent or productive HI
Seroconversion to simian immunodeficiency virus (SIV) in two laboratory workers.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 9. Unique Identifier : AIDSLINE PRIM10/93201064; Khabbaz R; Rowe T; Murphey-Corb M; Heneine W; Schable C; George JR; Pau C; Parekh B; Folks T; Centers for Disease Control, Atlanta, GA 30333.; SIV infection can cause AIDS in macaque monkeys. We report on two laboratory workers who seroconverted to SIV after exposures in SIV-research laboratories. The first is an animal worker who was accidentally stuck with a needle used to collect blood from a macaque monkey experimentally infected with SIV. Serial serolog
Regulatory elements in the long terminal repeat (LTR) of simian foamy virus type 3 (SFV-3).: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 90. Unique Identifier : AIDSLINE PRIM10/93201065; Renne R; Mergia A; Renshaw-Gegg LW; Neumann-Haefelin D; Luciw PA; University of California, Davis 95616.; Spumaviruses, or foamy viruses, are one of seven genera in the retroviridae family, and unique isolates have been recovered from many mammalian species including cats, hamsters, cows, sea lions, several non-human primates and man. Simian foamy virus type 3 (SFV-3), isolated from African green monkeys, has a complex ge
A molecular clone of STLV-I isolated from a diseased African green monkey.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 91. Unique Identifier : AIDSLINE PRIM10/93201066; Traina-Dorge V; Blanchard J; Murphey-Corb M; Tulane Regional Primate Research Center, Covington, LA.; Human T-cell leukemia virus (HTLV-I) is a type C retrovirus associated with adult T-cell leukemia. Seroepidemiological evidence suggests almost all species of Old World monkeys are naturally infected with the closely related simian virus, STLV-I. STLV-I can immortalize monkey cells in vitro. However, no direct viral l
Identification of a generalised packaging sequence for D-type retroviruses and generation of a D-type retroviral vector.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 92. Unique Identifier : AIDSLINE PRIM10/93201067; Vile RG; Ali M; Hunter E; McClure MO; Department of Communicable Diseases, St. Mary's Hospital Medical; School, London.; In order to construct vectors based upon D-type, rather than C-type, retroviruses, a 624 bp fragment of Mason Pfizer monkey virus (MPMV) has been identified, which can serve as a packaging sequence for at least two D-type retroviruses. When this fragment is included in a h
Analysis of the TNF-beta gene in rhesus macaques and sooty mangabeys.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 93. Unique Identifier : AIDSLINE PRIM10/93201068; Villinger F; Folks T; Mayne A; Vuchetich M; Findley H; Ansari AA; Dept. of Pathology, Emory Univ. School of Medicine, Atlanta, GA.; The regulation of HIV-1 and SIV virus replication in vitro by cytokines has been studied by a number of laboratories. Cytokines such as TNF have been shown to markedly upregulate HIV-1 replication. Recent data have shown that there is a functional polymorphism in the TNF-beta gene in humans which results in individual
Correlation of pathological findings at necropsy with a novel immunological evaluation and staging system in SIV-infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 94. Unique Identifier : AIDSLINE PRIM10/93201069; Amieiro JP; Del Llano AM; Lavergne JA; Kraiselburd E; Kessler MJ; Malaga CA; Animal Resources Center, University of Puerto Rico.; A group of eight juvenile Rhesus macaques, infected with different concentrations of SIVsm/E660 (10(4.8)TCID50/ml) were euthanized fifteen month post-infection. Pathological findings at the time of necropsy were correlated with a novel staging system based on a tri-parametric immunological evaluation recently develope
Titration in pig-tailed macaques of the HIV-1 IIIB vaccine virus challenge stock.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 95. Unique Identifier : AIDSLINE PRIM10/93201070; Benveniste R; Agy M; Katze M; Corey L; Hu SL; Tsai CC; Morton W; Arthur L; NCI, Frederick, MD.; The observation that pig-tailed macaques (M. nemestrina) can be infected with HIV-1 (Science 257: 103, 1992) has raised the possibility that this primate species may become useful for the evaluation of HIV-1 vaccines and antiviral therapies. Recent HIV-1 vaccine trials in chimpanzees have used HIV-1 IIIB (lot 040) as
Quality control in immunophenotyping of nonhuman primate AIDS animal models.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 96. Unique Identifier : AIDSLINE PRIM10/93201071; Brodie AR; Brown AS; McClure HM; Yerkes Primate Research Center, Emory University, Atlanta, GA.; A critical determination in AIDS patients and in nonhuman primate AIDS animal models is the determination of CD4+ lymphocytes. This value is used to monitor disease progression and response to therapy, and should be reported in absolute numbers. The latter depends on determining the percent of lymphocytes in a differe
Quantification of intraocular inflammatory responses to subretinal inoculation of rhesus CMV in eyes of normal and SIV immunosuppressed rhesus macaques.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 97. Unique Identifier : AIDSLINE PRIM10/93201072; Conway MD; Peyman GA; Murphey-Corb M; Soike KF; Bohn R; Martin L; LSU Eye Center, Louisiana State University Medical Center School; of Medicine, New Orleans 70112.; Three rhesus macaque monkeys (Macaca mulatta) were injected subretinally with 100 microliters of rhesus cytomegalovirus resuspended in phosphate buffered saline to a titer of 10(4) pfu/ml. Two animals were immunosuppressed as a result of infection with SIV/Delta B670 as evidenced b
Immunological and virological effects of opiate dependency in rhesus macaques: studies on SIV/SMM infection.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 98. Unique Identifier : AIDSLINE PRIM10/93201073; Donahoe RM; Bryd L; McClure HH; Fultz P; Brantley M; Marsteller F; Ansari AA; Aceto M; Emory University, Atlanta, GA.; A 2-1/2 year study was conducted to determine the effect of morphine on the immunological status of rhesus monkeys in the presence and absence of infection with the sooty mangabey strain of simian immunodeficiency virus (SIV/SMM). Opiate dependency was maintained by injection of 3mg/kg morphine per monkey, every 6 hr.
Susceptibility of macaque T lymphocytes and monocyte/macrophages to HIV-1.: Symp Nonhum Primate Models AIDS. 1992 Nov 17-20;10:abstract no. 99. Unique Identifier : AIDSLINE PRIM10/93201074; Gartner S; Liu Y; Rosenberg Y; Lewis M; Zack P; Eddy G; Henry M. Jackson Foundation Research Laboratory, Rockville,; Maryland 20850.; Peripheral blood mononuclear cells from a number of pig-tailed, cynomolgus and rhesus macaques were screened in parallel in vitro for susceptibility and permissivity to several strains of HIV-1. Cultures of monocyte/macrophages (MM) as well as T lymphocytes, were monitored for extracellular HIV-1 p24 production. Polym
Efficacy of ivermectin in the treatment of strongyloidiasis complicating AIDS.: Abstr Gen Meet Am Soc Microbiol. 1992;92:11 (abstract no. A-62). Unique Identifier : AIDSLINE ASM92/93201075; Torres J; Isturiz R; University Hospital, Universidad Central de Venezuela.; Strongyloides stercoralis is endemic in tropical regions worldwide. At least six documented cases of disseminated disease and AIDS have been reported. In the immunocompromised host, persistent or relapsing complications of extra-intestinal infection may ensue despite adequate standard courses of treatment. Recently, i
Enhancement of HTLV-I/II, HIV-I and HLA-DQ alpha PCR amplification efficiency using uracil-N-glycosylase.: Abstr Gen Meet Am Soc Microbiol. 1992;92:116 (abstract no. D-121). Unique Identifier : AIDSLINE ASM92/93201076; Kinard S; Butcher A; Wang Z; Spadoro J; Roche Diagnostic Systems, Fair Lawn, New Jersey.; Uracil-N-Glycosylase (UNG) has been used to prevent the occurrence of false positives due to the carryover of PCR amplified DNA. We determined the effects of UNG on amplification efficiency in PCR-based, colorimetric detection systems for HTLV-I/II, HIV-I and HLA-DQ alpha. Peripheral blood mononuclear cells were isola
Antigenic and DNA analyses of different clinical isolates of Mycoplasma penetrans, a new species of mycoplasma from patients with AIDS.: Abstr Gen Meet Am Soc Microbiol. 1992;92:159 (abstract no. G-2). Unique Identifier : AIDSLINE ASM92/93201077; Wang RY; Hayes M; Kotani H; Newton P 3d; Shih J; Lo SC; ARP, Armed Forces Inst. of Pathol., Wash. D.C.; An unusual organism (GTU-54), isolated from urine of an HIV+ male homosexual, has remarkable pathobiological properties of adhesion, hemadsorption and cytadsorption known to be associated with in vivo virulence. Biochemical, ultrastructural, antigenic and DNA studies revealed it to be a previously unknown mycoplasma t
Comparison of urogenital mycoplasmas found in the urine of AIDS and non-AIDS individuals.: Abstr Gen Meet Am Soc Microbiol. 1992;92:159 (abstract no. G-1). Unique Identifier : AIDSLINE ASM92/93201078; Hayes MM; Dawson MS; Wang R; Pierce P; Shih J; Lo SC; ARP, AFIP, Wash. DC.; Mycoplasma fermentans has been shown to be associated with AIDS-associated nephropathy in HIV+ patients and has been isolated from and detected by the polymerase chain reaction (PCR) in the urine of a number of HIV+ patients. To determine the extent of M. fermentans and other genital mycoplasmas found in HIV+ individu
Recovery of mycoplasma in respiratory cultures from HIV+ and HIV- patients: effect of isolation medium.: Abstr Gen Meet Am Soc Microbiol. 1992;92:160 (abstract no. G-3). Unique Identifier : AIDSLINE ASM92/93201079; Antik N; Wakefield T; Debiase J; Britton B; Tully J; Rose D; Oldach D; Mundy L; Auwaerter P; Burton A; et al; Johns Hopkins Med. Institutions, Baltimore, Md.; Throat swabs from patients with community acquired pneumonia, transported in SP 4 medium were stored at -70 degrees C until processed. Of > 450 samples, 11/1/90-10/30(91, 94 have been processed in 4 media. To date, 40 are positive (43 isolates), 45 negative, 9 in progress. Recoveries compared in Hanna RS flasks (HF),
Characterization of a new drug-resistant Mycobacterium species associated with pulmonary disease in AIDS patients.: Abstr Gen Meet Am Soc Microbiol. 1992;92:169 (abstract no. U-24). Unique Identifier : AIDSLINE ASM92/93201080; Yakrus M; Butler W; Kilburn J; Plikaytis B; Moss C; Silcox V; Floyd M; Vadney F; Gross W; Centers for Disease Control, Atlanta, Ga.; Seventeen isolates of biochemically identical, drug-resistant strains classified as either Mycobacterium avium complex or M. xenopi were further characterized. Specimen sources were mainly pulmonary, with seven isolates from AIDS patients. Testing included high-performance liquid chromatography (HPLC) of mycolic acids
Fastidious mycobacteria resembling M. simiae from AIDS patients.: Abstr Gen Meet Am Soc Microbiol. 1992;92:169 (abstract no. U-25). Unique Identifier : AIDSLINE ASM92/93201081; Coyle MB; Carlson LC; Wallis CK; Leonard RB; Raisys V; Harborview Medical Center, Seattle, WA.; Slow-growing fastidious mycobacteria were recovered from blood cultures of 7 AIDS patients within 2 years. The median time to detection in Bactec 13A broth was 28 days longer than for M. avium complex. Isolates failed to grow on routine subcultures to solid media, but grew in broth media including Middlebrook 7H9 and
Isolation of fastidious mycobacteria from AIDS patients.: Abstr Gen Meet Am Soc Microbiol. 1992;92:169 (abstract no. U-26). Unique Identifier : AIDSLINE ASM92/93201082; Jackson KM; Sievers AS; Ross BC; Dwyer BW; Fairfield Hospital, Victoria, Australia.; We have isolated four fastidious mycobacteria from AIDS patients since 1988. These 4 strains were initially isolated in BACTEC 12B and/or BACTEC 13A broths and failed to grow on routine Lowenstein-Jensen medium and egg yolk agar. The first patient had acid fast bacilli isolated from an abdominal lymph node, blood and
A comparative study of Mycobacterium avium complex (MAC) isolated from immunocompromised and immunocompetent patients and from environmental sources. 1. Recovery of MAC from Los Angeles potable water.: Abstr Gen Meet Am Soc Microbiol. 1992;92:170 (abstract no. U-32). Unique Identifier : AIDSLINE ASM92/93201083; Froman S; Glover N; Holtzman A; Aronson T; Berlin OG; Dominguez P; Anderson S; Overturf G; Olive View Med. Ctr, Sylmar, CA.; Los Angeles water is being examined as a possible source of MAC infection in immunocompromised (mainly AIDS) and immunocompetent patients. These studies will include 14 reservoirs supplying 90% of the city s water, 8 major hospitals and 50 homes. One liter water samples were collected in sterile polypropylene bottles
Dissemination of enteric Mycobacterium avium infections in mice infected with MAIDS retrovirus.: Abstr Gen Meet Am Soc Microbiol. 1992;92:170 (abstract no. U-28). Unique Identifier : AIDSLINE ASM92/93201084; Furney SK; Orme IM; Department of Microbiology, Colorado State University, Fort; Collins 80523.; Disseminated M. avium infections in AIDS patients is associated with CD4 T cell deficiency, and probably arises initially as a gut infection. We report here a new model of M. avium infection that may prove useful both in investigating the pathogenesis of the disease, and in testing new anti-mycobacterial drugs. Mice w
Incidence of mycobacterial infections in HIV positive intravenous drug abusers: analysis of 246 necropsy cases.: Abstr Gen Meet Am Soc Microbiol. 1992;92:170 (abstract no. U-30). Unique Identifier : AIDSLINE ASM92/93201085; Kaminski Z; Pearce M; Lombardo J; Goode R; Bloch A; Shilkret K; Lyons M; New Jersey Medical School-UMDNJ, Newark.; The object of this study is to determine whether HIV-Positive, intravenous drug abusers (IVDA) from the Newark, N.J. area who were autopsied at the Office of the N.J. State Medical Examiner were more likely to be culture positive for mycobacteria than the controls consistin