Synthesis of site-specifically modified oligoribonucleotides for studies of the recognition of TAR RNA by HIV-1 tat protein and studies of hammerhead ribozymes.

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Synthesis of site-specifically modified oligoribonucleotides for studies of the recognition of TAR RNA by HIV-1 tat protein and studies of hammerhead ribozymes.

Nucleic Acids Symp Ser. 1991;(24):55-8. Unique Identifier : AIDSLINE MED/93027432
Slim G; Pritchard C; Biala E; Asseline U; Gait MJ; MRC Laboratory of Molecular Biology, Cambridge, UK.

Abstract: Synthetic oligoribonucleotides having single uracil residues replaced by dU, dT, 2'-O-methylU or 5-bromodU have been prepared and used in the study of the interaction of HIV-1 tat protein with an RNA stem-loop. The preparation of phosphoramidites of 5-bromouridine and purine riboside suitable for use in solid-phase oligoribonucleotide synthesis is also described. The effect of adenine replacement by purine in a hammerhead ribozyme has also been determined.

Keywords: Base Sequence Gene Products, tat/*METABOLISM HIV-1/*METABOLISM Indicators and Reagents Molecular Sequence Data Nucleic Acid Conformation Oligoribonucleotides/*CHEMICAL SYNTHESIS RNA-Binding Proteins/*METABOLISM RNA, Catalytic/CHEMISTRY/*METABOLISM Support, Non-U.S. Gov't Uracil/*ANALOGS & DERIVATIVES JOURNAL ARTICLE
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M9311146

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