Oligonucleotides and their derivatives as tools for investigations of protein-nucleic acid interactions in template biocatalysis. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Oligonucleotides and their derivatives as tools for investigations of protein-nucleic acid interactions in template biocatalysis.

Nucleic Acids Symp Ser. 1991;(24):185-8. Unique Identifier : AIDSLINE MED/93027307
Lavrik OI; Institute of Bioorganic Chemistry, Siberian Division, Academy of; Sciences of USSR, Novosibirsk.

Abstract: On the basis of quantitative characteristics (Kd, Km, Gibbs energy values) for the interaction of oligonucleotides with template and primer site of eucaryotic and procaryotic DNA polymerases, a general model of template-primer interaction with these enzymes was suggested. The interactions of AMV- and HIV- reverse transcriptases with various 5'-derived oligonucleotides and with human DNA polymerase alpha and Klenow fragment are compared. The results obtained suggest a method to improve selectively the affinity of an oligonucleotide primer to RNA template with AMV- and HIV-reverse transcriptases.
Keywords: Base Composition Base Sequence Catalysis DNA Polymerase I/*METABOLISM Human HIV/ENZYMOLOGY Kinetics Molecular Sequence Data Myeloblastosis Virus, Avian/ENZYMOLOGY Nucleic Acids/*METABOLISM *Oligodeoxyribonucleotides Proteins/*METABOLISM RNA-Directed DNA Polymerase/*METABOLISM Templates Thermodynamics JOURNAL ARTICLEKWDbasecompositionbasesequencecatalysisdnapolymerasei/KWDmetabolismhumanhiv/enzymologykineticsmolecularsequencedatamyeloblastosisvirus,avian/enzymologynucleicacids/KWDmetabolismKWDoligodeoxyribonucleotidesproteins/KWDmetabolismrna-directeddnapolymerase/KWDmetabolismtemplatesthermodynamicsjournalarticle
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M9311128

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