Expression of a peptide related to the murine mammary tumor virus (MTV) but not to the human MTV in BALB/c cells of the B lineage (Meeting abstract).

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Expression of a peptide related to the murine mammary tumor virus (MTV) but not to the human MTV in BALB/c cells of the B lineage (Meeting abstract).

FASEB J; 6(5):A1706 1992. Unique Identifier : AIDSLINE ICDB/93687601
Lopez-Cepero M; Keydar I; Dion A; Riley R; Lopez DM; Dept. of Microbiology and Immunology, Univ. of Miami Sch. of; Medicine, Miami, FL 33136

Abstract: Splenic B cells from BALB/c mice express murine MTV (MMTV)-related protein on their cell membranes. This has been demonstrated by flow cytometry and by Western blots, using polyclonal anti-MMTV antibody. This protein has a mol wt of 68 kD and shares antigenic moieties with the env proteins gp52 and gp36. BALB/c bone marrow B220+ sIg- cells also display the 68-kD protein on their surface. In mouse and human DNA, there is a sequence coding for a protein related to MMTV gp52. There is a 60% homology between these sequences in mouse and human; however, the peptides vastly differ. C2-(M) and C2-(H) are synthetic peptides derived from sequences which, respectively, encode for a portion of MMTV gp52 and of a human MTV-gp52-related molecule. Polyclonal antibodies against these C2 peptides were used to further explore the nature of the 68-kD protein expressed on BALB/c B cells. Fluorescence analyses show that these cells stain specifically with the anti-C2-(M) antibody but not with the anti-C2-(H). Western blots of splenic B-cell membrane preparations, using the anti-C2-(M) antibody, detect a protein of mol wt comparable to the one previously detected with the polyclonal anti-MMTV antibody. However, no protein band could be seen when anti-C2-(H) antibody was used. These results indicate that the mouse C2 peptide NH2-Trp Glu Asn Ser Pro Lys Asp Pro Asn Asp Arg Asp-COOH is contained within the protein preferentially expressed on B lymphocytes of BALB/c mice and may serve as a tool for detecting cells of the B lineage at various stages of differentiation.

Keywords: Amino Acid Sequence Animal B-Lymphocytes/*METABOLISM Gene Products, env/METABOLISM Mammary Tumor Viruses, Mouse/GENETICS/*METABOLISM Mice Mice, Inbred BALB C Neoplasm Proteins/GENETICS/*METABOLISM Peptides/METABOLISM Spleen/CYTOLOGY ABSTRACT
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M9320864

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