Lack of evidence for the presence of human immunodeficiency virus type 1-related sequences in patients with Graves' disease. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Lack of evidence for the presence of human immunodeficiency virus type 1-related sequences in patients with Graves' disease.

Thyroid. 1991 Winter;1(4):307-14. Unique Identifier : AIDSLINE MED/93044563
Tominaga T; Katamine S; Namba H; Yokoyama N; Nakamura S; Morita S; Yamashita S; Izumi M; Miyamoto T; Nagataki S; First Department of Internal Medicine, Nagasaki University School; of Medicine, Japan.

Abstract: The pathogenesis of human autoimmune diseases, including Graves' disease, remains to be elucidated. Recently, Ciampolillo et al. proposed that human immunodeficiency virus type 1 (HIV-1)-related retroviruses were involved in Graves' disease. Using Southern blot analysis, they found specific integration of exogenous sequences homologous to HIV-1 gag region in genome DNA of thyrocytes and peripheral blood lymphocytes (PBL) of patients with Graves' disease. In order to test their hypothesis, we examined the presence or the absence of HIV-1 gag-related sequences in Japanese Graves' patients using the polymerase chain reaction (PCR) in addition to Southern blotting. Sets of primer pairs used in PCR were designed to cover the whole span of the HIV-1 gag region. Hybridization was performed in both relaxed and stringent conditions. Our results showed that neither Southern blot hybridization nor PCR gave positive signals in any of the samples examined from Graves' patients. This suggests that HIV-1 or its closely associated viruses are unlikely to be involved in the pathogenesis of Graves' disease in Japan.
Keywords: Adolescence Adult Base Sequence Blotting, Southern DNA, Viral/ANALYSIS Female Gene Products, gag/GENETICS Graves' Disease/GENETICS/*MICROBIOLOGY Human HIV-1/GENETICS/*ISOLATION & PURIF Male Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes/GENETICS Polymerase Chain Reaction Support, Non-U.S. Gov't JOURNAL ARTICLEKWDadolescenceadultbasesequenceblotting,southerndna,viral/analysisfemalegeneproducts,gag/geneticsgraves'disease/genetics/KWDmicrobiologyhumanhiv-1/genetics/KWDisolation&purifmalemolecularsequencedatanucleicacidhybridizationoligonucleotideprobes/geneticspolymerasechainreactionsupport,non-uKWDsKWDgov'tjournalarticle
930228
M9320857

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1993. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1993. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .