Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

Nucleic Acids Symp Ser. 1991;(25):79-80. Unique Identifier : AIDSLINE MED/93065322
Izuta S; Saneyoshi M; Sakurai T; Suzuki M; Kojima K; Yoshida S; Laboratory of Cancer Cell Biology, Nagoya University School of; Medicine, Japan.

Abstract: Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.
Keywords: Animal Antiviral Agents/*PHARMACOLOGY Cattle DNA Polymerase III/*ANTAGONISTS & INHIB HIV/DRUG EFFECTS Male Thymine Nucleotides/*PHARMACOLOGY Zidovudine/*ANALOGS & DERIVATIVES/PHARMACOLOGY JOURNAL ARTICLEKWDanimalantiviralagents/KWDpharmacologycattlednapolymeraseiii/KWDantagonists&inhibhiv/drugeffectsmalethyminenucleotides/KWDpharmacologyzidovudine/KWDanalogs&derivatives/pharmacologyjournalarticle
930228
M9320851

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