Oligodeoxyribonucleotide phosphorothioate fluxes and localization in hematopoietic cells. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Oligodeoxyribonucleotide phosphorothioate fluxes and localization in hematopoietic cells.

Antisense Res Dev. 1992 Spring;2(1):27-39. Unique Identifier : AIDSLINE MED/93044239
Marti G; Egan W; Noguchi P; Zon G; Matsukura M; Broder S; Division of Biochemistry and Biophysics, Food and Drug; Administration, Bethesda, Maryland.

Abstract: An antisense oligonucleotide phosphorothioate, previously shown to inhibit HIV-1 viral expression in chronically infected H9 cells, was fluorescently labeled to study oligonucleotide fluxes and localization within living cells. Observations based on flow cytometry and fluorescence microscopy show the following: within around 0.5-2 h, an apparent steady-state distribution of the oligonucleotide is achieved in which the intracellular oligonucleotide concentration is less than that present in the external medium; following oligonucleotide uptake and resuspension of the cells in oligonucleotide-free medium, an oligonucleotide efflux, with a time constant similar to that for uptake, is observed (although a significant fraction of the phosphorothioate remains within the cell); cellular uptake as a function of the external oligonucleotide concentration is nonlinear, being more efficient at lower concentrations (less than 2 microM); and a predominant oligonucleotide localization within the cell nucleus and perinuclear organelles is observed.
Keywords: Base Sequence Biological Transport Cell Line Flow Cytometry Hela Cells Human Indicators and Reagents Kinetics Microscopy, Fluorescence Molecular Sequence Data Oligonucleotides, Antisense/CHEMICAL SYNTHESIS/*METABOLISM Support, U.S. Gov't, P.H.S. Thiophosphoric Acid Esters/*METABOLISM Tumor Cells, Cultured JOURNAL ARTICLEKWDbasesequencebiologicaltransportcelllineflowcytometryhelacellshumanindicatorsandreagentskineticsmicroscopy,fluorescencemolecularsequencedataoligonucleotides,antisense/chemicalsynthesis/KWDmetabolismsupport,uKWDsKWDgov't,pKWDhKWDsKWDthiophosphoricacidesters/KWDmetabolismtumorcells,culturedjournalarticle
930228
M9320734

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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