Stimulation of peripheral blood mononuclear cells (PBMNCs) from chronic lymphatic leukemia (CLL) patients by an antisense phosphorothioate oligomer to the rev gene of HIV (Meeting abstract). NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Stimulation of peripheral blood mononuclear cells (PBMNCs) from chronic lymphatic leukemia (CLL) patients by an antisense phosphorothioate oligomer to the rev gene of HIV (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A2713 1993. Unique Identifier : AIDSLINE ICDB/93693657
Branda RF; Moore AL; Mathews L; McCormack JJ; Zon G; Dept. of Medicine, Vermont Cancer Center, Univ. of Vermont,; Burlington, VT 05401

Abstract: Serious infections are frequent complications of CLL. We observed that an antisense oligomer designed to treat HIV infection caused splenomegaly and hypergammaglobulinemia in mice. This anti-rev 27-mer also is a potent B-cell mitogen and induces production of high titers of IgM and IgG in normal mice in vivo, and in normal mouse splenocytes and normal human PBMNCs in vitro. We now report that similar immunostimulatory effects were seen with an anti-rev 21-mer phosphorothioate but not with a 20-mer p53 antisense oligomer or a 28-mer anti-rev phosphodiester. Moreover, this 27-mer oligomer is active in cells from about 50% of CLL patients tested. PBMNCs from responding patients after exposure to anti-rev oligomer had stimulation indices of 15 to 117 and produced higher levels of IgM and IgG in vitro than the same cells treated with pokeweed mitogen. This novel agent may be useful to enhance gamma globulin production and thereby prevent the infections which occur in CLL.
Keywords: Animal Genes, rev/*GENETICS Human IgG/ANALYSIS IgM/ANALYSIS Leukemia, Lymphocytic, Chronic/*IMMUNOLOGY Leukocytes, Mononuclear/*IMMUNOLOGY *Lymphocyte Transformation Mice ABSTRACTKWDanimalgenes,rev/KWDgeneticshumanigg/analysisigm/analysisleukemia,lymphocytic,chronic/KWDimmunologyleukocytes,mononuclear/KWDimmunologyKWDlymphocytetransformationmiceabstract
931230
M93C0830

Copyright © 1993 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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