Homology of human immunodeficiency virus 1 (HIV-1) sequences to human DNA mapped by in situ hybridization (Meeting abstract).

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Homology of human immunodeficiency virus 1 (HIV-1) sequences to human DNA mapped by in situ hybridization (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A3240 1993. Unique Identifier : AIDSLINE ICDB/93694196
Popescu NC; Zimonjic DB; DiPaolo JA; Lab. of Biology, NCI, Bethesda, MD 20892

Abstract: To map the virus integration site in a human T-cell line (ACH-2) with a single copy of defective virus, chromosomes were hybridized with a biotinylated HIV-1 DNA probe (Oncor, Gaithersburg, MD). The HIV integration was localized on the short arm of chromosome 7 but several other chromosomes exhibited signal at the centromere. Using the same probe, chromosomes from peripheral leukocytes of several healthy donors were hybridized and the sites of homology of HIV-1 DNA probe to human DNA were mapped at the centromeric region of chromosomes 1, 11, 17 and X. Suppression hybridization did not abrogate signals at any of the 4 centromeric sites. However, high stringency posthybridization washings resulted in labelling of the centromeric region on only chromosome 17, indicating that this region has the highest degree of sequence homology to HIV-1 DNA probe.

Keywords: Chromosomes, Human, Pair 7 DNA DNA Probes DNA, Viral Human HIV-1/*GENETICS/PHYSIOLOGY In Situ Hybridization *Sequence Homology, Nucleic Acid Virus Integration ABSTRACT
931230
M93C0828

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1993. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1993. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .