Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens.

Mem Inst Oswaldo Cruz. 1992 Apr-Jun;87(2):239-47. Unique Identifier : AIDSLINE MED/93375900
Caterino-de-Araujo A; Secao de Imunologia, Instituto Adolfo Lutz, SP, Brasil.

Abstract: The present report describes an alternative method for in vitro detection of HIV-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analyzed. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.
Keywords: Adolescence Adult Blotting, Western Child Child, Preschool Comparative Study Enzyme-Linked Immunosorbent Assay Female Human HIV Antibodies/IMMUNOLOGY/*ISOLATION & PURIF HIV Antigens/IMMUNOLOGY HIV Seropositivity/*IMMUNOLOGY HIV-1/*IMMUNOLOGY Infant Middle Age Pregnancy Sensitivity and Specificity Support, Non-U.S. Gov't Time Factors JOURNAL ARTICLEKWDadolescenceadultblotting,westernchildchild,preschoolcomparativestudyenzyme-linkedimmunosorbentassayfemalehumanhivantibodies/immunology/KWDisolation&purifhivantigens/immunologyhivseropositivity/KWDimmunologyhiv-1/KWDimmunologyinfantmiddleagepregnancysensitivityandspecificitysupport,non-uKWDsKWDgov'ttimefactorsjournalarticle
931230
M93C0781

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