Detection of cytomegalovirus in urine of HIV-infected patients by DNA-DNA hybridization comparison with virus isolation, immunofluorescence and immunoperoxidase. NLM AIDSLINE Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

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Detection of cytomegalovirus in urine of HIV-infected patients by DNA-DNA hybridization comparison with virus isolation, immunofluorescence and immunoperoxidase.

Mem Inst Oswaldo Cruz. 1992 Jan-Mar;87(1):99-102. Unique Identifier : AIDSLINE MED/93375889
Valdivia A; Marrero M; Alvarez M; Mune M; Valdes O; Roges G; Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba.

Abstract: Immunofluorescence and immunoperoxidase test directed against early viral antigens, and DNA-DNA hybridization were compared with viral isolation for their abilities to detect Cytomegalovirus (CMV) in the urine of 89 HIV infected patients. From the 100 urine samples collected, 70 were found positive by at least one method. Considering viral isolation as the gold standard technique, immunofluorescence and immunoperoxidase had a sensitivity of 92.3% and 88% respectively, with a specificity in both cases of 95%. DNA-DNA hybridization showed a sensitivity of 90% but with lower (60%) specificity. All of the three assays were effective in detecting CMV from urine and the technical advantage of each is discussed.
Keywords: Antibodies, Viral/*URINE Comparative Study Cytomegalovirus/*ISOLATION & PURIF Cytomegalovirus Infections/COMPLICATIONS/DIAGNOSIS/*URINE DNA, Viral Fluorescent Antibody Technique Human HIV Infections/COMPLICATIONS/*URINE Immunoenzyme Techniques Nucleic Acid Hybridization Sensitivity and Specificity JOURNAL ARTICLEKWDantibodies,viral/KWDurinecomparativestudycytomegalovirus/KWDisolation&purifcytomegalovirusinfections/complications/diagnosis/KWDurinedna,viralfluorescentantibodytechniquehumanhivinfections/complications/KWDurineimmunoenzymetechniquesnucleicacidhybridizationsensitivityandspecificityjournalarticle
931230
M93C0767

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