Establishment of a leukemic T-cell line expressing a conformationally different but functional IL-2R (Meeting abstract).

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Establishment of a leukemic T-cell line expressing a conformationally different but functional IL-2R (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A322 1993. Unique Identifier : AIDSLINE ICDB/93691308
Sahraoui Y; Thedoropoulo M; Ammar A; Clemenceau C; Jasmin C; Georgoulias V; INSERM U268, Hopital Paul Brousse, Villejuif, France

Abstract: Leukemic cells from a 45-yr-old male patient with an acute lymphoblastic leukemia of T-cell origin were cultured in vitro in the absence of any added growth factor for up to 2 yr and a continuous cell line (NOI-90) was established. Phenotypic and karyotypic analysis of NOI-90 cells revealed only small differences from initial leukemic cells. NOI-90 cells expressed a low amount of the IL2R alpha and IL-2R beta of IL-2R, respectively, and scatchard analysis revealed the presence of a small number of high-affinity IL-2R, which was unchanged during a 18-mo period. The NOI-90 cells secrete a biological IL2 activity and the specific mRNA could be detected by PCR. Although added IL2 could not induce cell proliferation, monoclonal antibodies (MoAb) against IL2R alpha, IL2R beta and IL2, as well as an immunosuppressive molecule, p29, secreted by HIV-infected adherent cells, could inhibit spontaneous cell proliferation in a dose-dependent manner. The effect of MoAb against IL-2R beta on the spontaneous cell proliferation is not observed on normal activated T-cells (PHA-blasts) in the same conditions. The antiproliferative effect at 37 C of anti-IL2R beta is quite striking, since in normal PHA-blasts, 3.58 pM IL2 displaces the moAbs, whereas in NOI-90 cells 3580 pM, IL2 does not displace the anti-IL2R beta. These data suggest in a particular subset of T-ALL patients, the existence of an heterodimer with a different configuration defining a thousand increased affinity of this epitope for this MoAb.

Keywords: Cell Division/GENETICS Cell Line Gene Expression Regulation, Leukemic/*PHYSIOLOGY Human Leukemia, T-Cell, Acute/*GENETICS/PATHOLOGY Male Middle Age Protein Conformation Receptors, Interleukin-2/*GENETICS ABSTRACT
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M9380837

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