Identification and culture of Kaposi's sarcoma-like spindle cells from the peripheral blood of HIV-1-infected individuals and normal controls (Meeting abstract).

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Identification and culture of Kaposi's sarcoma-like spindle cells from the peripheral blood of HIV-1-infected individuals and normal controls (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A1122 1993. Unique Identifier : AIDSLINE ICDB/93692093
Browning PJ; Sechler JM; Washington RH; Yarchoan R; Gallo RC; LTCB, NCI, Bethesda, MD 20892

Abstract: Kaposi's sarcoma (KS) is a multifocal proliferative disorder of endothelial cells. To investigate why KS is multifocal, equal numbers of peripheral blood mononuclear cells from 48 HIV-1+ patients (pts) with and without KS and 27 HIV-1- normal controls were cultured in the presence of activated lymphocyte-conditioned medium. We grew spindle-shaped adherent cells that had an endothelial phenotype expressing the following surface markers: Ulex europeaus lectin+, EN7/44+, BNH9+, alpha-smooth muscle actin+, CD13+, CD34+, CD36+, HLA-DR+; however, they were Factor VIIIR-. These cells were phenotypically similar to some of the spindle cells found in KS tumors. The number of spindle cells cultured per cm2 of culture surface area, from the peripheral blood of HIV-1+ pts with KS and HIV-1+ pts at high risk for developing KS (eg, homosexual and bisexual males, female sexual partners of bisexual males), was increased 79-fold and 24-fold, respectively, when compared with HIV-1- controls. There was no increase in the number of spindle cells cultured from HIV-1+ pts at low risk for developing KS (eg, heterosexual males, women and iv drug abusers), when compared with HIV-1- controls. The increased number of spindle cells cultured from HIV-1+ pts with KS and HIV-1+ high-risk pts may explain the multifocal nature of KS, and may provide new leads regarding the etiology of KS.

Keywords: Cells, Cultured Endothelium, Vascular/PATHOLOGY Female Human HIV Infections/*PATHOLOGY HIV Seropositivity/PATHOLOGY *HIV-1 Male *Neoplasm Circulating Cells Risk Factors Sarcoma, Kaposi's/*PATHOLOGY Skin Neoplasms/*PATHOLOGY Tumor Cells, Cultured/*PATHOLOGY ABSTRACT
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M9380835

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