Expression of transcriptional factors in highly metastatic and nonmetastatic human rhabdomyosarcoma (RMS) (Meeting abstract).

Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.

Expression of transcriptional factors in highly metastatic and nonmetastatic human rhabdomyosarcoma (RMS) (Meeting abstract).

Proc Annu Meet Am Assoc Cancer Res; 34:A3006 1993. Unique Identifier : AIDSLINE ICDB/93693951
Kalebic T; Slade C; Rosenthal L; Zeichner S; Helman L; NCI, Pediatric Branch, Bethesda, MD 20892

Abstract: A model was developed to investigate whether highly metastatic cells and nonmetastatic cells differentially express specific transcriptional factors. Highly metastatic and nonmetastatic cell variants were isolated by in vitro cloning of a human RMS cell line RD. The HIV-LTR, containing a number of defined binding regions for transcriptional factors including AP-1, NFAT-1, NFkB, SP-1 was used as a reporter DNA sequence to study the binding of transcriptional factors from RD-4A and RD-LF cell lines. HIV-LTR-CAT was transfected into RD-4A or RD-LF recipient cell lines and the level of transcriptional activity was determined. In addition, HIV-LTR-CAT harboring a mutation in the NFkB binding region was transfected into highly metastatic and nonmetastatic cells. Gel-shift analysis was performed by using the DNA NFkB binding region produced by PCR as a probe and nuclear extracts from RD-4A and RD-LF cells. Our results show that the transcriptional activity of the HIV-LTR is 8- to 10-fold higher in highly metastatic cells than in nonmetastatic cells. In addition, while the mutation in the NFkB binding region remarkably reduces the level of HIV-LTR transcriptional activity in the nonmetastatic subclone RD-LF, the level of transcriptional activity in the highly metastatic clone RD-4A remained unchanged. These data strongly support our hypothesis that in highly metastatic and nonmetastatic cells qualitative and quantitative differences exist in expression of transcriptional factors.

Keywords: Chloramphenicol Acetyltransferase/GENETICS DNA, Neoplasm/METABOLISM Human HIV Long Terminal Repeat/*GENETICS NF-kappa B/METABOLISM Rhabdomyosarcoma/*GENETICS/SECONDARY Transcription Factors/METABOLISM Transcription, Genetic Tumor Cells, Cultured ABSTRACT
930830
M9380827

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