Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
Inhibition of HTLV-I entry in peripheral blood mononuclear cells by monoclonal antibody 34-23 (Meeting abstract).
FASEB J; 6(4):A1296 1992. Unique Identifier : AIDSLINE ICDB/93687101 Fan N; Poiesz BJ; Gavalchin J; Silverstone A; State Univ. of New York Health Science Center, Syracuse, NY 13210
Abstract:
A monoclonal IgM antibody, MAb 34-23, that identifies a cell surface antigen on cells susceptible to infection by HTLV-I has been produced and its ability to block HTLV-I binding to target cells and inhibit cell-free HTLV-I entry into target cells examined. Preincubation of PHA-activated peripheral blood mononuclear cells (PBMS) with 3.0 ug of MAb 34-23 prior to the addition of rhodamine-18-labeled HTLV-I virions (R-HTLV-I) results in a 15-channel decrease in mean channel fluorescence (log scale) when cell fluorescence is analyzed by flow cytometry. The decrease in binding of R-HTLV-I by MAb 34-23 was nearly equivalent to that observed when target cells were preincubated with 50 ug of P19-normalized, unlabeled HTLV-I. Furthermore, recombinant HTLV-I env protein inhibited MAb 34-23 binding to target cells in a competitive ELISA assay. Next, a PCR-based cell-free HTLV-I infection assay was developed to test the effect of MAb 34-23 on HTLV-I infection of target cells. Preincubation of target cells with MAb 34-23 reduced significant HTLV-I entry in PBMS. When the target cells were preincubated with 100, 50, 10 or 2 ug of MAb 34-23, the HTLV-I proviral DNA signal generated after infection was reduced by 91%, 82%, 43% and 31%, respectively. No inhibition was observed when MOPC 104E, a control IgK antibody, was used as the inhibitor. The antigen identified by MAb 34-23 was analyzed by Western blot, and 4 bands of mol wt 32, 45, 58 and 75 kD were identified. Interestingly, both HTLV-I virions and recombinant HTLV-I env protein were found to react with 3 proteins of mol wt 32, 45 and 75 kD, similar to those identified with MAb 34-23. Recombinant HTLV-I env protein also reacts with affinity-purified Mab 34-23 when analyzed by SDS-PAGE and Western blot. Taken together, these data suggest that MAb 34-23 may identify a putative cellular receptor(s) for HTLV-I.
Keywords: *Antibodies, Monoclonal Antigens, Viral/IMMUNOLOGY Gene Products, env/IMMUNOLOGY HTLV-I/*IMMUNOLOGY/PHYSIOLOGY Leukocytes, Mononuclear/*IMMUNOLOGY Virus Replication ABSTRACT 930430
M9340830
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Important note: Information in this article was accurate in 1993. The state of the art may have changed since the publication date.
