Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
DETECTION AND CHARACTERIZATION OF T-CELL LEUKEMIA VIRUS-LIKE PROVIRAL SEQUENCES IN PBLS AND TISSUES OF BABOONS BY PCR. A NEW T-CELL LEUKEMIA/LYMPHOMA TYPE I RETROVIRUS (MEETING ABSTRACT)
Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 86, 1991.. Unique Identifier : AIDSLINE ICDB/92682426 Schatzl H; Yakovleva L; Rose D; Inzhiia L; Lapin B; Deinhardt F; von der Helm K; Max von Pettenkofer Inst., Univ. of Munich, Munich, Germany
Abstract:
The 'high lymphoma-prone' baboon stock (Papio hamadryas) of the Sukhumi Primate Center colony (USSR) is characterized by a high seroprevalence of antibodies to the STLV-I/HTLV-I type retrovirus. It is further characterized by a manifestation of nearly 80-100% of T-cell malignancies. In contrast, in other primate colonies and in wild living monkeys, at most 20% of the animals had antibodies, and only a very low incidence of T-cell lymphomas was found. One explanation for this particularity could be that, originally, the Sukhumi stock derived from a baboon which was inoculated with human lymphomatous material. To elucidate this particularity, DNA analysis of peripheral blood lymphocytes and various tissues of healthy, risked and ill Papio hamadryas baboons was performed by polymerase chain reaction (PCR). A fair correlation between serological and PCR data and symptoms of T-cell malignancy was obtained. Furthermore, the distribution of proviral DNA was investigated in different tissues. For precise identification and characterization of the Sukhumi T-cell lymphoma virus, parts of the virus genome were mapped by restriction enzyme sites and PCR-derived fragments. A 350-nucleotide fragment of the env gene revealed 52 base exchanges to HTLV-I (84% homology) and 48 to STLV-I (86% homology), including one deletion of a triplet. On the level of aa sequence, this indicated an exchange of 4 aa to STLV-I (3.4%), but only of 2 aa to HTLV-I (1.7%). Various isolates of HTLV-I are highly conserved (97-99%; also in the env region) and HTLV-I and STLV-I are homologous more than 90%; these results may indicate that the Sukhumi T-cell lymphoma virus might be a new representative of the T-cell leukemia/lymphoma virus type I family. Furthermore, the infected monkeys from Sukhumi, which develop T-cell malignancy at a high rate, might serve as a 'model' for the highly latent and prolonged pathogenesis of HTLV-I-associated T-cell leukemia/lymphoma in humans.
Keywords: Animal Chromosome Deletion Chromosome Mapping Gene Amplification Gene Products, env/GENETICS Genes, Viral/GENETICS Genome, Viral HTLV-I/*GENETICS Leukemia, T-Cell/GENETICS Lymphoma, T-Cell/*VETERINARY Monkey Diseases/*GENETICS Papio Polymerase Chain Reaction Proviruses/*GENETICS Retroviridae Infections/*GENETICS Sequence Homology, Nucleic Acid STLV/*GENETICS ABSTRACT 920930
M9290914
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
